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All Journal Jurnal Natur Indonesia Al-Manahij: Jurnal Kajian Hukum Islam Indonesian Journal of Educational Studies Jurnal Sains Dasar INSPIRASI Jurnal Akuntansi Multiparadigma Jurnal Ilmiah Setrum Jurnal Edukasi dan Penelitian Informatika (JEPIN) PROSIDING SEMINAR KIMIA SOCIETAL KAREBA : Jurnal Ilmu Komunikasi The Journal of Educational Development SemanTIK : Teknik Informasi Omni-Akuatika Insect (Informatics and Security): Jurnal Teknik Informatika Jurnal BALIRESO Jurnal Perikanan Universitas Gadjah Mada SENTRALISASI ACTIVE: Journal of Physical Education, Sport, Health and Recreation Prosiding Temu Profesi Tahunan PERHAPI JIIP (Jurnal Ilmiah Ilmu Pendidikan) Infotek : Jurnal Informatika dan Teknologi
Alimuddin Alimuddin
Jurusan Teknik Elektro, Universitas Sultan Ageng Tirtayasa Cilegon, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Mathematics Nursing Public Health Social Sciences Other

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Journal : Jurnal Perikanan Universitas Gadjah Mada

 1 
Penggunaan Gen GH sebagai Marka Molekuler DNA Gurami, Osphronemus goramy dalam Pengembangan Teknologi Surrogate Broodstock Achmad, Marlina; Alimuddin, Alimuddin; Carman, Odang; Arfah, Harton; Zairin, Muhammad
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (34.127 KB) | DOI: 10.22146/jfs.7

Abstract

The technology of fish germ cell transplantation had been established to create broodstock systems by which a target offspring can be produced from a surrogate parent. Donor cell for transplantation is derived from transgenic fish carrying green fluorescent protein gene functions as a marker to distinguish the donor from recipient cell. In this study, an alternative technique was developed for identifying gouramy-derived donor cell and Nile tilapia as recipient by PCR amplification method using growth hormone (GH) gene as a molecular marker.  Specific primer for GH gouramy was designed by using Genetyx version 7 software.  ß-actin gene was used as an internal control of DNA loading. The result showed that a specific PCR amplification product of 340 in length was obtained when the DNA template was from gouramy, while no PCR product from Nile tilapia. The minimum concentration of genomic DNA of gouramy mixed with a 700 ng/µl of Nile tilapia that could be detected by PCR was 1 ng/µl. Thus, PCR method with specific GH primer may be useful to detect the incorporation of donor cell in recipient gonad towards development of surrogate broodstock technology.
Co-Authors Adi Susanto Aman Sentosa Panggabean Aris Munandar Ayi Santika Bohari Yusuf Budiaji, Weksi Chairul Saleh Desy Ridho Rahayu Dewi Anggraini Diah Ayu Gustiningsih Fachriyan Hasmi Pasaribu Filus, Tio Firmansyah Dahlan Firzhal Arzhi Jiwantara Grahita Chandrarin Gugus Irianto Harjoni Desky Harton Arfah Hasanuddin, Muh Iqbal Hasnawati Hasnawati Herjayanto, Muh. Ibrahim Ibrahim Iwan Triyuwono Jasmanindar, Yudiana Jumaing, Muhammad Kahar, Irsan Kardi, Ipa Sari Komaini, Anton Komar Sumantadinata Kusumawati, Andi La Surimi, La Liana Sari, Ika Yekti M. Zairin Junior Marlina Achmad Mediaty Millenia, Tasya Muhammad Nadjib, Muhammad Muhammad Ruslan Muhammad Zairin N., R.R. Dirgarini Julia Nasution Nasution Natalis Ransi, Natalis Nur Bambang Priyo Utomo Nur Fitria, Nur Odang Carman Rahmat Ramadhan Rasyidah Jalil Retno Damayanti Soejoedono Sajiah, Adha Mashur Saputra, Anke Rienhar Satria, Teguh Septiana, Rina Soerja Koesnarpadi Sri Nuryati Sukenda Sukenda Sulsalman Moita, Sulsalman Tjetjep Rohendi Rohidi, Tjetjep Rohendi Trisnawati, Ani Wijaya, Lalu Kerta YUNI, YUNI Zulkarnain, Dimas Allan