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Keragaman warna bunga m1 tanaman aster (Callistephus chinensis) Hasil induksi mutasi iradiasi sinar gamma Yanuar Rizqiani; Florentina Kusmiyati; Syaiful Anwar
Journal of Agro Complex Vol 2, No 1 (2018)
Publisher : Department of Agriculture, Animal and Agricultural Sciences, Diponegoro University, Semara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (579.07 KB) | DOI: 10.14710/joac.2.1.52-58

Abstract

The aims of research was to determine the effect of gamma ray on the flower colour of Daisies. The research design was completely randomized design with five replicates. The irradiation treatment of gamma ray were 0 Gy, 5 Gy, 10 Gy, 15 Gy, 20 Gy. Parameters observed were stalk length, time of flowering, number of flowers, flower diameter, and flower colour. The collected data were analyzed by analysis of variance (ANOVA) and continued by Least Significance Different (LSD) of 5% level. The result showed that irradiation of gamma ray did not affected stalk length, number of flowers, and time of flowering. The gamma ray irradiation had a significant effect on flower diameter. Gamma ray irradiation significantly decreased the diameter of flower. The flower colour at doses 0 Gy (control) was purple. Colour flower was varied from dark purple to pink at irradiation 10 Gy and 15 Gy. Keywords: Callistephus chinensis, mutation, irradiation, colour of flower.
Perkecambahan benih dan pertumbuhan bibit jarak pagar (Jatropha curcas Linn.) dengan invigorasi menggunakan zat pengatur tumbuh pada periode simpan yang berbeda Indah Puspitaningtyas; Syaiful Anwar; Karno Karno
Journal of Agro Complex Vol 2, No 2 (2018)
Publisher : Department of Agriculture, Animal and Agricultural Sciences, Diponegoro University, Semara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (296.542 KB) | DOI: 10.14710/joac.2.2.148-154

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Jatropha curcas is a plant that is used as an alternative energy for fossil fuel replacement because of it’s high oil content. Jatropha curcas seeds have 30-40% oil content. The purpose of the study was to improve the seed quality and to enhance seedling growth of Jatropha curcas after storage using plant growth regulator. This study used factorial design based on Completely Randomized Design with two factors and 3 replications. The first factor was seed storage which consist of 2 levels : A1 = seed storage in 2015, A2 = seed storage in 2016. The second factor was plant growth regulator level which consists of 6 levels : B1=GA+NAA 0 ppm, B2= GA+NAA 20 ppm, B3= GA+ NAA 40 ppm, B4= GA+NAA 60 ppm, B5 = GA+NAA 80 PPM, B6 = GA+NAA 100 ppm. Parameters measured were seed germination, vigor index, growth rate, plant height and the number of leaves. The data were analyzed statistically by analysis of variance and followed by Tukey test and Polynomial Orthogonal. The result showed that seed germination, vigor index and growth rate were significantly affected by storage treatment and invigoration using plant growth regulator treatment. Invigoration using auxin and gibberellic acid 40 ppm has the highest result of seed germination, vigor index and growth rate. There was no influence of seed storage and invigoration with plant growth regulator treatment on plant height and number of leaves. Keywords : Jatropha curcas, invigoration, plant growth regulator.
Aplikasi paclobutrazol dan pelapisan lilin lebah terhadap mutu rimpang benih jahe merah (Zingiber officinale var. Rubrum) selama penyimpanan Maulana Azhar Adipraja; Syaiful Anwar; Florentina Kusmiyati
Journal of Agro Complex Vol 3, No 3 (2019): Online
Publisher : Department of Agriculture, Animal and Agricultural Sciences, Diponegoro University, Semara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.938 KB) | DOI: 10.14710/joac.3.3.124-131

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Jahe merupakan tanaman yang bagian rimpangnya memiliki nilai ekonomi yang tinggi. Penelitian ini bertujuan untuk mengkaji pengaruh aplikasi paclobutrazol dan pelapisan lilin lebah dalam mempertahankan mutu rimpang benih jahe merah selama penyimpanan. Penelitian dilaksanakan pada bulan April – Juli 2018 di Laboratorium Fisiologi dan Pemuliaan Tanaman, Fakultas Peternakan dan Pertanian, Universitas Diponegoro, Semarang. Penelitian ini merupakan percobaan faktorial 4x4 dengan 3 kali ulangan dengan dasar Rancangan Acak Lengkap (RAL). Faktor pertama adalah konsentrasi paclobutrazol dengan 4 taraf, terdiri atas 0, 500, 1000, 1500 ppm. Faktor kedua adalah konsentrasi lilin lebah dengan 4 taraf, terdiri atas 0, 4, 6, dan 8 %. Penyimpanan dilakukan pada kondisi ruang simpan dengan suhu 28-30°C selama tiga bulan. Penyemaian dilakukan selama satu bulan setelah masa penyimpanan. Parameter yang diamati adalah jumlah rimpang bertunas, tinggi tunas, susut bobot rimpang dan kadar air rimpang. Data dianalisis menggunakan analisis ragam dan uji lanjut menggunakan Duncan Multiple Range Test (DMRT). Berdasarkan penelitian yang telah dilakukan diperoleh hasil bahwa perlakuan paclobutrazol dengan konsentrasi 500 ppm dan perlakuan lilin lebah konsenrasi 4% dapat menghambat tinggi tunas, mempertahankan jumlah rimpang bertunas, susut bobot rimpang dan kadar air rimpang hingga akhir masa simpan.Kata kunci: jahe merah, paclobutrazol, lilin lebah.
Pola segregasi karakter agronomi tanaman kacang panjang (Vigna unguiculate (l.) spp. sesquipedalus) generasi F2 hasil persilangan varietas Super Putih x Fagiola IPB Adinda Rachmawati; Syaiful Anwar; Karno Karno
Journal of Agro Complex Vol 4, No 2 (2020): JOAC Online
Publisher : Department of Agriculture, Animal and Agricultural Sciences, Diponegoro University, Semara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/joac.4.2.79-88

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  ABSTRACTThe purpose of this research was to analyze the segregation pattern of agronomic characters in the second generation of long beans produced by crossing Super Putih x Fagiola IPB varieties. The research was using a single plant design by planting 120 F2 generation plants and 10 old plants were plated without repetition. The parameters observed included plant height, leaf number, branch number, flowering age, long young pod, number of old pods, seed number, weight of 100 seeds, pods color, and seeds color. The data were analyzed by distribution suitability test and chi-square test. The result showed that the abnormal distributed characters were plant height and seed number with selection could be done in early generation because its have high inheritance. Plant height segregation patterns was referring to Mendelian ratio 9:7 with the action of two duplicate recessive epistatic genes, seed number patterns was referring to Mendelian ratio 12:3:1 with the action of the dominant epistatic gene, the color of pods and the color of seeds patterns was referring to Mendelian ratio 13:3 with the action of two recessive dominant epistatic genes. Key words: long beans, agronomic characters, segregation patternsABSTRAKPenelitian ini bertujuan untukmenganalisis pola segregasi karakter agronomi generasi F2 kacang panjang hasil persilangan varietas Super Putih x Fagiola IPB. Penelitian disusun dengan rancangan single plant dengan menanam sebanyak 120 tanaman generasi F2 dan tetua sebanyak 10 tanaman yang ditanam tanpa ulangan. Parameter yang diamati yaitu tinggi tanaman, jumlah daun, jumlah cabang, umur berbunga, panjang polong muda, jumlah polong tua, jumlah biji per tanaman, bobot 100 biji, warna polong, dan warna biji. Data dianalisis dengan uji kesesuaian distribusi dan uji khi-kuadrat. Hasil penelitian menunjukkan bahwa karakter yang tidak berdistribusi normal adalah tinggi tanaman dan jumlah biji, sehingga seleksi dapat dilakukan pada generasi awal, karena memiliki daya waris yang tinggi. Pola segregasi tinggi tanaman mengikuti nisbah Mendel 9:7 dengan aksi dua gen epistatis resesif duplikat, jumlah biji mengikuti nisbah Mendel 12:3:1 dengan aksi dua gen epistatis dominan, sedangkan warna polong dan biji mengikuti nisbah Mendel 13:3 dengan aksi dua gen epistatis dominan resesif.Kata kunci : kacang panjang, karakter agronomi, pola segregasi 
Karakter Fisiologi dan Pendugaan Heritabilitas Tanaman M1 Bawang Merah (Allium Ascalonicum L.) Hasil Induksi Iradiasi Sinar Gamma Lika Alfariatna; Florentina Kusmiyati; Syaiful Anwar
Journal of Agro Complex Vol 2, No 1 (2018)
Publisher : Department of Agriculture, Animal and Agricultural Sciences, Diponegoro University, Semara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.379 KB) | DOI: 10.14710/joac.2.1.19-28

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ABSTRACTThe aim of the research was to determine the physiological characters, heritability estimation, and information of lethal doses (LD50) of M1 onion plant induced by gamma ray irradiation. The research was arranged in monofactor experimental with Complete Randomized Design (CRD) with 6 treatments and 5 replication, each replication consisted of 5 onion bulbs was irradiated by gamma ray of 0, 3, 6, 9, 12, 15 Gy. Parameters observed: the percentage of germination, chlorophyl content, nitrate reductase activity, heritability estimation and LD50. The data were analyzed by anova and followed by BNT 5% level. The result showed that irradiation significant affected to chlorophyl content and nitrate reductase activity. LD50 of onion is 7,64 Gy. Heritability value range from 52,22 – 80,51%. Keywords : Allium ascalonicum L.,irradiation, LD50, heritability
Karakteristik morfologi dan sitologi tanaman Sutra Bombay (Portulaca grandiflora Hook) hasil poliploidisasi dengan kolkisin pada berbagai konsentrasi dan frekuensi aplikasi Betty Perdana Sari; Karno Karno; Syaiful Anwar
Journal of Agro Complex Vol 1, No 2 (2017)
Publisher : Department of Agriculture, Animal and Agricultural Sciences, Diponegoro University, Semara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2154.457 KB) | DOI: 10.14710/joac.1.2.39-48

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Moss Rose (Portulaca grandiflora Hook) is one of the ornamental plants and medicinal plants. The purpose of this research was to evaluate cytological and morphological responses of Moss Rose (Portulaca grandiflora) with colcichine treatment. Colcichine treatment was done with drop method technique in different concentrations and different frequency of application. This research used Completely Randomized Design in 4x3 factorial arrangement and used descriptive analysis to compare diploid plant and tetraploid plant characters. The first factor was variation of cholcichine concentration which were K0 (0%), K1 (0.1%), K2 (0.3%), and K3 (0.6%). The second factor was variation of application frequency which were A1 (2 drop per day for a day), A2 (2 drop per day for 2 days), and A3 (2 drop per day for 3 days). The observed parameters were shoot response by colcichine treatment, number of chromosomes, length and width of stomata, stomata density, stem diameter, flower diameter, plant high, fresh weight, number of branches, and number of leaves. The result showed that Moss Rose’s shoot was intolerant with colcichine treatment in high concentration and long time application. The tetraploid plants was randomly produced by a number of treatment which were 0.1% colcichine 2 drops for 3 days, 0.3% colcichine 2 drops for 2 days, 0.6% colcichine 2 drops for 2 days, and 0.6% colcichine 2 drops for 3 days. The shoot’s growth was inhibited. The induced of tetraploid plant in Moss Rose was accompanied by double chromosome number as 2n=4x=36, larger stomata (length and width), lower stomata density, and higher morphological characters. Moss Rose (Portulaca grandiflora Hook) is one of the ornamental plants and medicinal plants. The purpose of this research was to evaluate cytological and morphological responses of Moss Rose (Portulaca grandiflora) with colcichine treatment. Colcichine treatment was done with drop method technique in different concentrations and different frequency of application. This research used Completely Randomized Design in 4x3 factorial arrangement and used descriptive analysis to compare diploid plant and tetraploid plant characters. The first factor was variation of cholcichine concentration which were K0 (0%), K1 (0.1%), K2 (0.3%), and K3 (0.6%). The second factor was variation of application frequency which were A1 (2 drop per day for a day), A2 (2 drop per day for 2 days), and A3 (2 drop per day for 3 days). The observed parameters were shoot response by colcichine treatment, number of chromosomes, length and width of stomata, stomata density, stem diameter, flower diameter, plant high, fresh weight, number of branches, and number of leaves. The result showed that Moss Rose’s shoot was intolerant with colcichine treatment in high concentration and long time application. The tetraploid plants was randomly produced by a number of treatment which were 0.1% colcichine 2 drops for 3 days, 0.3% colcichine 2 drops for 2 days, 0.6% colcichine 2 drops for 2 days, and 0.6% colcichine 2 drops for 3 days. The shoot’s growth was inhibited. The induced of tetraploid plant in Moss Rose was accompanied by double chromosome number as 2n=4x=36, larger stomata (length and width), lower stomata density, and higher morphological characters. 
Pengaruh keragaman jamur Metarhizium anisopliae terhadap mortalitas larva hama Oryctes rhinoceros dan Lepidiota stigma Syafira Athifa; Syaiful Anwar; Budi Adi Kristanto
Journal of Agro Complex Vol 2, No 2 (2018)
Publisher : Department of Agriculture, Animal and Agricultural Sciences, Diponegoro University, Semara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.251 KB) | DOI: 10.14710/joac.2.2.120-127

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The research was conducted to evaluate the effect of variant of M. anisopliae on mortality of Oryctes rhinoceros and Lepidiota stigma. Variants ofM. anisopliae to be used were Karimun Jawa, Semarang and Magelang (host O. rhinoceros), and UGM (host L. stigma). The research was arranged in factorial experiment withcompletely randomized design, two factors and three replications.The first factor was type variant of M. anisopliae (V0= control, V1= variant UGM, V2= variant Karimun Jawa , V3= variant Semarang , V4 = variant Magelang), the second factor was type of pest (H1 = larva O. rhinoceros, H2 = larva L. stigma). The results showed that each variant of M. anisopliae had the same ability to control O. rhinoceros and L. stigma in both mortality, spore density, and LT50. O. rhinoceros has higher mortality and spore density than L. stigma, but LT50 both pests were the same. The interaction between O. rhinoceros and M. anisopliae gave the highest mortality, spore density, but the same LT50. Keywords : M. anisopliae, L. stigma, O. rhinoceros, mortality, spore density, LT50.
Keragaman M1 tanaman hias bunga matahari (Helianthus annuus l.) akibat iradiasi sinar gamma Intan Novela Setya Monikasari; Syaiful Anwar; Budi Adi Kristanto
Journal of Agro Complex Vol 2, No 1 (2018)
Publisher : Department of Agriculture, Animal and Agricultural Sciences, Diponegoro University, Semara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (835.85 KB) | DOI: 10.14710/joac.2.1.1-11

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ABSTRACT The purpose of the research was to obtain the morphological variability of M1 sunflower ornamental plant and information of lethal doses (LD50) effect of mutation by gamma ray irradiation. The research was arranged in monofactor experimental with Completely Randomized Design (RAL) with 5 treatments and 5 replication, each replication consisted of 5 sunflower seeds was irradiated by gamma ray of 0, 5, 25, 45, and 65 Gy. The data observed were analyzed by anova and followed with BNT of 5% level. Parameters observed included germination, plant height, stem diameter, tube flower diameter, tape flower diameter, initiation time, perfect bloom age, chlorophyll, ribbon flower number, seed number, total seed weight, weight of 100 seeds, LD50, Flower color and heritability. The result showed that irradiation significant effected to plant height, stem diameter, tube flower diameter, ribbon flower diameter, initiation time, seeds number, seed weight, weight of 100 seeds. Lethal doses of sunflower is 74,19 Gy. Heritability value range from 23,47 to 73,81%.Keywords : Helianthus annus L., irradiation, LD50, heritability
Aplikasi ekstrak daun tapak dara (Catharanthus roseus l.) terhadap pertumbuhan dan morfologi tanaman bawang daun (Allium fistulosum l.) A'yuni Fatkhi Fajriyati; Syaiful Anwar; Florentina Kusmiyati
Jurnal Ilmiah Pertanian Vol. 19 No. 1 (2022): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v19i1.9445

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Welsh onion (Allium fistulosum L.) is one of the horticultural commodity crops with high consumer value in Indonesia, but production remains low. Efforts to increase the production of Welsh onion can be performed by induction of anti-mitotic substances. Catharanthus roseus leaves contain anti-mitotic alkaloid compounds that promote growth and yield. This research aimed to analyze the effect of extract C. roseus leaf on the growth and morphology of Welsh onion. This research was conducted in Kejajar, Wonosobo, Central Java, followed by analysis in Plant Physiology and Breeding Laboratory, Diponegoro University. This research used a Randomized Block Design (RBD) factorial with three concentrations; 0.05%, 0.1%, and 0.2%, and three levels of soaking duration; 6, 9, and 12 hours. The data were analyzed statistically using ANOVA followed by Post-hoc Test using Honestly Significant Different (HSD) at 5%. The result showed that applying 0.05% C. roseus leaf extract significantly affected sprouting time, number of tillers per plant, number of leaves per plant, fresh weight, and stomatal density. In addition, the interaction between 0.1% C. roseus leaf extract treatment and 12 hours of soaking duration increased root length by 16.67 cm.
APLIKASI BERBAGAI KONSENTRASI GIBERELIN DAN KOMPOSISI MEDIA AKAR PAKIS PADA PERTUMBUHAN DAN HASIL PANEN TANAMAN KAILAN (Brassica oleracea L.) Nawang Nurfaizi Muzahid; Karno; Syaiful Anwar
Jurnal Agrotech Vol 11 No 2 (2021)
Publisher : FAKULTAS PERTANIAN UNIVERSITAS ALKHAIRAAT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31970/agrotech.v11i2.66

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Penelitian dilakukan di rumah kaca mini yang terletak di Babakan, Bogor, Jawa Barat dan Laboratorium Fisiologi dan Pemuliaan Tumbuhan Fakultas Peternakan dan Pertanian Universitas Diponegoro, dari bulan Desember 2021 sampai dengan Februari 2021. hormon giberelin dan penambahan akar pakis sebagai media tanam terhadap pertumbuhan dan produksi brokoli cina (Brassica oleracea L.). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial 4x3 dengan 5 ulangan. Faktor pertama adalah konsentrasi hormon giberelin (H): H0 (0 ppm), H1 (50 ppm), H2 (100 ppm), dan H3 (150 ppm). Faktor kedua adalah penambahan akar pakis sebagai media tanam (A) : A0 (tanpa akar pakis), A1 (1 lapis akar pakis), dan A2 (2 lapis akar pakis). Parameter yang diamati adalah kenampakan tunas pertama, tinggi tanaman, jumlah daun, diameter batang, luas daun, bobot segar total, bobot segar konsumsi, bobot segar akar, bobot kering total, bobot kering tajuk tanaman, bobot kering akar dan persentase massa kering. Hasil penelitian menunjukkan bahwa pemberian hormon giberelin konsentrasi 150 ppm dan tanpa penambahan akar pakis memberikan hasil terbaik pada bobot segar total dan bobot segar konsumsi. Hasil penelitian juga menunjukkan bahwa konsentrasi hormon giberelin 0 ppm dan 1 lapis akar tumbuhan paku memberikan hasil persentase massa kering yang paling baik.
Co-Authors - Universitas Bina Sarana Informatika, Frans Eduard Schaduw A'yuni Fatkhi Fajriyati Adinda Rachmawati Adriani Darmawati Afriani, Imro'atul Husna Agus Jatmiko Ahmad Ghanaim Fasya, Ahmad Ghanaim Ahmadi, Asep Aisyah Ufairoh Rahmah Amalia Wulannanda Ambarwati, Dessy Anak Agung Istri Sri Wiadnyani Anang M Legowo Asmoro, Novky Ayningtiyas, Allivia Ayutha Wijiindyah Badrud Tamam Begum Fauziyah Betty Perdana Sari Buchory, Gina Nuraini C.T, Nanda Ayu Caribu Hadi Prajitno, Caribu Hadi Dandy E Prasetiyo, Dandy E Devita Sari, Ni Nengah Diana Nur Afifah, Diana Nur Dwi Retno Lukiwati Eduard Schaduw, Frans Ekowati Chasanah Ekowati, Titik Endang Dwi Purbajanti Eny Fuskhah Eny Yulianti, Eny Erlianti, Eka Fajar Risqi Yuniarti Fatkhurrozi, Mu’amar Fiorentina Chelsea Firda Lailatus Sa’adah Florentina Kusmiyati Florentina Kusmiyati Galih, Mardella Gina Nuraini Buchory Gustaf, Muhammad Akbar Maulana H., Septiana Putri Hesti Reva Helva Ari Zahmi Indah Puspitaningtyas Indrawati, Rosita Indriani Indriani Intan Novela Setya Monikasari Irwan Hermanan K. Karno Kartiwi, Asti Putri Kartono, Rano Kaswari . Kirmi, Hifzil Kristanto, Budi Adi Kristanto, Budi Adi Langkah Sembiring Lika Alfariatna Luky Adrianto Lulu Fatikhatul Maryamah M. Syamsul Maarif Mastar Asran Maulana Azhar Adipraja Maya Oktavia, Maya Melia Sari, Melia Moch Arif Usman mubarok, tasdik Muhammad Masyhuri Mujito Mujito, Mujito Munasik Munasik Muti'ah, Roihatul Nafiatul Khoyriyah Nanik Hamidah Nanik Nurhana narmiana, narmiana Nawang Nurfaizi Muzahid Nelvia Iryani Ninik Rustanti Noor Fitriya Mirta Liana Novita Dwi Lisdyayanti Dwi Lisdyayanti Nugroho, Indra Nurhalimah, Kiki Nurhana, Nanik Nurhayati, Endah Nurmila Karimah Nurul Shintawati Pambudi, Enggar Dias Pujoyuwono Martosuyono Puspa, Novita Trie Ana R. Djoko Soetrisno Rahmadani, Melinia Refita Rizki Retno Murwani Riadhotus Saadati Rian Hilmawan Rizki Wahyu Kuncoro Roessali, Wiludjeng Rulia Ervina Dewi Sarah Zulfaniah Septaka T Riyadi Setyawan, Sendy Agus Siyamto, Andika Sri Darmawati Sri Hetty Susetyorini Sri Hetty Susetyorini Sumarsono Sumarsono Sutarno Sutarno Syafira Athifa Taufik Akbar Wayan T. Artama Widya Asmara Widyati Slamet Wijiindyah, Ayutha Yanu Andria Sucianto Yanuar Rizqiani Yosi Irene Putri Yuli Chomsatu Samrotun Yunita Hardi Setyanti Yusro Nuri Fawzya