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All Journal BULETIN OSEANOGRAFI MARINA Bioma : Berkala Ilmiah Biologi Jurnal Ilmu Lingkungan Jurnal Natur Indonesia Jurnal Akademika Biologi Microbiology Indonesia Jurnal Bioteknologi & Biosains Indonesia (JBBI) Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Penbios: Jurnal Pendidikan Biologi dan Sains International Journal of Research in Community Services Prosiding Konferensi Nasional PKM-CSR Makara Journal of Science AGRIC Open Access DRIVERset
Rejeki Siti Ferniah
Biotechnology Study Program , Biology Department, Faculty Of Sains And Natural Sciences, Diponegoro University
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Cloning of a Gene Encoding Protease from Bacillus halodurans CM1 into Escherichia coli DH5α and Expression Analyses of the Gene Product Helianti, Is; Furgeva, Natasha; Mulyawati, Lina; Ferniah, Rejeki Siti; Kusumaningrum, Hermin Pancasakti
Makara Journal of Science Vol. 22, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Bacillus halodurans strain CM1 is an Indonesia alkalothermophilic bacterium isolated from Cimanggu Hot Spring, Bandung, West Java. This bacterial strain produces high levels of thermoalkalophilic xylanase. It has also been predicted to produce other potential industrial enzymes, including protease. For production and application of protease in the future, the protease gene from B. halodurans CM1 was cloned into Escherichia coli. The protease gene was isolated from B. halodurans CM1 by the PCR approach using primers designed based on the GenBank. The PCR product was then ligated into pGEM-T Easy vector, transformed into E. coli DH5α, verified, and analyzed based on DNA sequencing data using the BLAST search tool. A 1086-bp protease gene was obtained that exhibited a very high sequence similarity (99%) with that of alkaline protease gene from B. halodurans C-125. When the culture of this positive recombinant E. coli DH5α containing the protease gene was spotted onto calcium caseinate agar, a clear zone appeared after incubation at 50 °C. This result demonstrated that the protease gene was expressed in this recombinant E. coli DH5α.
Characterization and Pathogenicity of Fusarium oxysporum as the Causal Agent of Fusarium Wilt in Chili (Capsicum annuum L.) REJEKI SITI FERNIAH; BUDI SETIADI DARYONO; RINA SRI KASIAMDARI; ACHMADI PRIYATMOJO
Microbiology Indonesia Vol. 8 No. 3 (2014): September 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (258.32 KB) | DOI: 10.5454/mi.8.3.5

Abstract

Fusarium wilt is a serious disease attacking chili plants in Central Java which cause lost of chili productivity. Fusarium wilt is caused by pathogenic fungi Fusarium oxysporum, which is host specific. The objectives of this research were to characterize the pathogenic F. oxysporum as the causal agent of fusarium wilt in chili plants and to observe the virulence of the pathogen. Fungal pathogen was isolated from Tawangmangu as an endemic area of fusarium wilt in Central Java. The fungi was characterized morphologically and identified molecularly by its internal transcribed spacer regions (ITS regions). Pathogenicity test was done to observe the virulence of the pathogen. One pathogenic strain was isolated from Tawangmangu, Karanganyar and was identified  morphologically and molecularly as F. oxysporum.  
Phylogenetic of Ornamented Chilli Based on the Fruit Morphology and Molecular Marker using OPA-12 Primer Rejeki Siti Ferniah; Endang Kusdiyantini; Nurhayati Nurhayati
Agric Vol. 30 No. 1 (2018)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2018.v30.i1.p57-62

Abstract

There are many varieties of ornamental chilli that may different from their ancestor and cause genetic variability. This research aims to determine the relationship of ornamental chillies in Indonesia based on their fruit morphology and RAPD-PCR. Morphological was analysed by description method, while molecular was analysed by RAPD-PCR using OPA 12 primer. Result showed that Katty, Naga Morich, Big Black Mama, and Yellow Primo were Capsicum chinense based on their morphology and RAPD-PCR using OPA-12 primer.
PERTUMBUHAN VEGETATIF TANAMAN CABAI PELANGI (Capsicum annuum L.) PADA PERLAKUAN PGPR (Plant Growth Promoting Rhizobacteria), KOMBINASI PGPR-PUPUK NPK, DAN PGPR-KOMPOS Jennefer Constantia; Rejeki Siti Ferniah
Agric Vol. 32 No. 2 (2020)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2020.v32.i2.p95-104

Abstract

Cabai Pelangi Bolivian Rainbow merupakan salah satu varietas cabai hias yang memiliki keunikan berupa warna buahnya yang berwarna-warni. Pertumbuhan vegetatif cabai hias ini memerlukan beberapa tambahan nutrisi dan peranan mikroorganisme untuk memperoleh pertumbuhan yang optimal. Penelitian ini bertujuan untuk mengetahui pertumbuhan optimal cabai pelangi menggunakan pemberian pupuk hayati PGPR, kombinasi dari pupuk hayati PGPR dan pupuk sintetik NPK, serta kombinasi pupuk hayati PGPR dan pupuk organik kompos. Penelitian ini dilakukan dengan Rancangan Acak Lengkap (RAL) faktor tunggal dengan 4 perlakuan. Keempat perlakuan tersebut adalah tanah hitam sebagai kontrol (P0), tanah hitam ditambah pemberian PGPR(P1), tanah hitam ditambah pupuk kompos + PGPR(P2), dan tanah hitam ditambah NPK + PGPR (P3). Data yang diperoleh kemudian dianalisis dengan ANOVA dan dilanjutkan dengan Uji BNT pada tahap uji á = 5%. Hasil penelitian menunjukkan perlakuan kombinasi PGPR dan kompos atau PGPR dan NPK memberikan pertumbuhan vegetatif optimal dibandingkan perlakuan tunggal PGPR.
Phylogenetic of Ornamented Chilli Based on the Fruit Morphology and Molecular Marker using OPA-12 Primer Rejeki Siti Ferniah; Endang Kusdiyantini; Nurhayati Nurhayati
Agric Vol. 30 No. 1 (2018)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2018.v30.i1.p57-62

Abstract

There are many varieties of ornamental chilli that may different from their ancestor and cause genetic variability. This research aims to determine the relationship of ornamental chillies in Indonesia based on their fruit morphology and RAPD-PCR. Morphological was analysed by description method, while molecular was analysed by RAPD-PCR using OPA 12 primer. Result showed that Katty, Naga Morich, Big Black Mama, and Yellow Primo were Capsicum chinense based on their morphology and RAPD-PCR using OPA-12 primer.
PERTUMBUHAN VEGETATIF TANAMAN CABAI PELANGI (Capsicum annuum L.) PADA PERLAKUAN PGPR (Plant Growth Promoting Rhizobacteria), KOMBINASI PGPR-PUPUK NPK, DAN PGPR-KOMPOS Jennefer Constantia; Rejeki Siti Ferniah
Agric Vol. 32 No. 2 (2020)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2020.v32.i2.p95-104

Abstract

Cabai Pelangi Bolivian Rainbow merupakan salah satu varietas cabai hias yang memiliki keunikan berupa warna buahnya yang berwarna-warni. Pertumbuhan vegetatif cabai hias ini memerlukan beberapa tambahan nutrisi dan peranan mikroorganisme untuk memperoleh pertumbuhan yang optimal. Penelitian ini bertujuan untuk mengetahui pertumbuhan optimal cabai pelangi menggunakan pemberian pupuk hayati PGPR, kombinasi dari pupuk hayati PGPR dan pupuk sintetik NPK, serta kombinasi pupuk hayati PGPR dan pupuk organik kompos. Penelitian ini dilakukan dengan Rancangan Acak Lengkap (RAL) faktor tunggal dengan 4 perlakuan. Keempat perlakuan tersebut adalah tanah hitam sebagai kontrol (P0), tanah hitam ditambah pemberian PGPR(P1), tanah hitam ditambah pupuk kompos + PGPR(P2), dan tanah hitam ditambah NPK + PGPR (P3). Data yang diperoleh kemudian dianalisis dengan ANOVA dan dilanjutkan dengan Uji BNT pada tahap uji á = 5%. Hasil penelitian menunjukkan perlakuan kombinasi PGPR dan kompos atau PGPR dan NPK memberikan pertumbuhan vegetatif optimal dibandingkan perlakuan tunggal PGPR.
Co-Authors Achmadi Priyatmojo Ade Fajrian Adhitya Naufal Pribadhi Agung Suprihadi Anggraini, Ika Anggraini, Ika Anto Budiharjo Arina Tri Lunggani Arina Tri Lunggani Athried Elsima Budi - Raharjo BUDI SETIADI DARYONO Candra Wahyuningsih Chiesa Salsabila Diana Ayu Fitriana Dinda Khairunnisa Eka Oktaviani Eka Oktaviani eko purnomo Elke Gildantia Endang Kusdiyantini Endang Kusdiyantini Furgeva, Natasha Hermin Pancasakti Kusumaningrum Hesti Tri Rahayu Himmatul Ulya Himmatul Ulya HUSNUL KHOTIMAH Ika Anggraini Ika Anggraini Anggraini Is Helianti IS HELIANTI Istiana, Rohma Jennefer Constantia Jumari Jumari, Jumari Khoirin Nida Lina Mulyawati MG Isworo Rukmi Muhammad Zainuri Mulyawati, Lina Natasha Furgeva Nurhayati Nurhayati Nurmalisa Rizko Rina Sari Asih Rina Sri Kasiamdari Sabrini, Zalia salamah salamah Shelina Nurunnisa Mawarni Siti Nur Jannah Siti Nur Jannah Sri Darmanti Sri Pujiyanto Sri Pujiyanto Sri Pujiyanto Sri Pujiyanto Sri Pujiyanto Sunarno s Supriyadi Supriyadi Susiana - Purwantisari Susiana Purwantisari Tia Erfianti Vivi Suryaningsih Weni Lestari, Weni Wijanarka Wijanarka Yumna Rahmadias Hanifa Yunnia Rahmandanni