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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) JSDH BERITA BIOLOGI Jurnal Biodjati BIOEDUSCIENCE Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Menara Perkebunan JPPI (Jurnal Penelitian Pendidikan Indonesia) MADURANCH: JURNAL ILMU PETERNAKAN Jurnal Ilmiah Sains
Hamim Hamim
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Mathematics Physics Veterinary

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Kandungan IAA, serapan hara, pertumbuhan dan produksi jagung dan kacang tanah sebagai respon terhadap aplikasi pupuk hayati Sigit Tri Wibowo; . Hamim; Aris Tri Wahyudi
Jurnal Ilmu Pertanian Indonesia Vol. 14 No. 3 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (895.569 KB)

Abstract

The aim of this research was to study IAA content, nutrient uptake, growth and productivity of maize and peanut in response to application of biofertilizer. The research was conducted in a green house of Cikabayan IPB Farm, Bogor Agriculture University, Darmaga, Bogor, West Java. A completely randomized design was applied in single factor experiment for maize and peanut with 3 replications. The treatments consisted of 4 factors: I. Without fertilizer, II. 100% biofertilizer (dosage 100g/pot), III. 100% inorganic fertilizer, and IV. Combination of biofertilizer and inorganic fertilizer with 50% dosage. Biofertilizer was applied using compost enriched by Pseudomonas sp., Bacillus sp., Azotobacter sp., Azospirillum sp., Rhizobium sp, and P-solubilising bacteria. The dosage of inorganic fertilizer was 0.5 gfpot of Urea; 0.5 g/pot of SP-36; 0.375 g/pot of KCI for maize, and 0.125 g/pot of Urea; 0.5 g/pot of SP-36; 0.375 g/pot of KCI for peanut. Application of biofertilizer enhanced auxin content of maize by 73-159°/o, but not in peanut. The treatment also increased the uptake of N, P, and K of both plants by 2 to 35 times as compared to control plant. The production increased by 270% on maize and 66% on peanut due to application of biofertilizer. The result showed that application of compost enriched by microbial activator was able to supplement inorganic fertilizer for growth and production of maize and peanut.
Analisis Morfofisiologi, Anatomi, dan Histokimia pada Lima Spesies Tanaman Gulma sebagai Respons terhadap Merkuri dan Timbal Rani Apriyani Raharja; Hamim Hamim; Yohana Caecilia Sulistyaningsih; Triadiati Triadiati
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 3 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.25.3.412

Abstract

Plants can be used as phytoremediation agents to improve critical land due to gold mining activities. This experiment aimed to analyze the morphophysiological, anatomical, and histochemical responses of Brachiaria mutica, Cyperus kyllingia, Ipomea aquatica, Mikania micrantha, and Paspalum conjugatum in response to the application of heavy metals mercury and lead in the forms of Hg(NO3)2 and Pb(NO3)2 in hydroponic experiments and to determine the ability of these plants to accumulate both metals. Morphological responses were observed by measuring number of leaves and plant dry weight, and physiological responses were observed by analyzing photosynthetic pigments, while anatomical and histochemical responses were analyzed by microscopic observation to tranversal slice of roots and leaves. The results showed that the applications of Hg(NO3)2 and Pb(NO3)2 treatments caused decreases in number of leaves, plant dry weights, and photosynthetic pigments (chlorophyll a, chlorophyll b, and carotenoid). The treatments also decreased leaf thickness due to the decrease in the epidermis, but they caused the increases in exodermis and endodermis of the roots. Mercury and lead were accumulated in large amounts in the roots, but accumulation in the shoot was less. Histochemical observation showed that lead was found in the roots of all the plants, especially in endodermic tissue and the vessel, whereas in the leaves the two metals were detected in the upper and lower epidermis, mesophyll, and vessel. Among the five species tested, C. kyllingia and P. conjugatum were the most tolerant to Pb and Hg.
Application of Biological Nitrogen Fixation Cyanobacteria To Paddy Plant Cultivated Under Deep-Water Culture System Dian Hendrayanti; Iman Rusmana; Dwi Andreas Santosa; Hamim Hamim
Jurnal Biodjati Vol 5, No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.8510

Abstract

 The Biological Nitrogen Fixing (BNF) cyanobacteria can reduce atmospheric nitrogen into ammonium. This ability makes BNF cyanobacteria a potential eco-friendly N-source for soil-planted pad-dy. Apart from a few success stories of BNF cyanobacteria applica-tion in the rice field, its role as an ammonium producer is still an open question. There is also a possibility that indeed cyanobacteria biomass which provides nitrogen through the biological decomposing process. This study aimed to analyze the influence of three strains BNF cyanobacteria on paddy grown in the Deep-Water Culture (DWC) hydroponic system. Yoshida Nutrient Solution was used as a growth medium with the application of 0, 45.7, and 91.4 gL-1 of ammonium nitrate (NH4NO3) with five replications. The result showed that inter-action between cyanobacteria and nitrogen significantly influenced the total nitrogen of paddy plant, but not the paddy plant height, the num-ber of tillers, and biomass. Cyanobacterial filaments were found most-ly attached to the paddy root rather than floated in the DWC system. Paddy treated with cyanobacteria had more lateral root than control. The result suggested that cyanobacteria support paddy growth indi-rectly through nitrogen deposition in plant tissue and root development. 
Enkapsulasi dan Regenerasi Kalus Embriogenik Mangga (Mangifera indica L.) Kultivar Bapang dan Gadung 21 Tri Muji Ermayanti; Robertus Nugroho; Hamim Hamim
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2598

Abstract

The mother plant and genetic variability of Indonesian mango need to be conserved. Encapsulation is one of in vitro conservation used for many plant species. The aim of this research was to study the regeneration of encapsulated mango cultivar Bapang and Gadung 21 embryogenic callus after storage at -14, 3−5, and 26−27oC for 0, 1, 2, 4, and 8 weeks. Embryogenenic callus was treated with 3% Na-alginate (in liquid 3M), then it was dropped into 100 mM CaCl2. Encapsulated callus beads were dehidrated and stored at -14, 3−5, and 26−27oC for 0, 1, 2, 4, and 8 weeks. After storage, the callus was cultured in 3M medium containing 2,4-D at 0, 1, and 2 mg/l for the regeneration. The results showed that after 8-week storage, callus of Bapang culticar was more viable (67.3%) and resulted more number of somatic embryos (191.6%) than Gadung 21 cultivar. The callus which was cultured in 3M medium without addition of 2,4-D was more viable (20.9%) and had more number of somatic embryos (1062.5%) than that which was cultured on medium containing 2,4-D. After 2-week storage, callus had viability of 7.6%. No storage callus formed more somatic embryos than storage callus. Storage at 26−27oC gave higher viability as well as higher number of somatic embryo than stored at -14 and 3−5 oC. The callus did not regenerate into shoots after storage at -14oC. Embryogenic callus could be stored at 3−5 and 26−27oC for 4 weeks.
Klorofil Floresen dari Spesies C3 dan C4 dalam Responnya terhadap Cekaman Kekeringan Hamim Hamim
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.2876

Abstract

Klorofil floresen fotosintesis dari dua spesies C3 (gandum dan kale) dan dua spesies C4 (Echinochloa cursgallii dan Amaranthus caudatus) dianalisis dalam responnya terhadap cekaman kekeringan di rumah kaca. Tumbuhan ditanam dalam pot berdiameter 15 cm selama sebulan kemudian diberi perlakuan kekeringan dengan penundaan penyiraman hingga tumbuhan layu. Kuantum efisiensi maksimum dari sistem cahaya II fotosintesis (Fv/Fm), quenching foto kimia (qP) dan non-fotokimia (qN) dianalisis untuk mengetahui keadaan fotosintesis tumbuhan selama cekaman kekeringan. Walaupun tidak ada pola yang jelas dalam hal status air dari spesies C3 dan C4, cekaman kekeringan yang diberikan menyebabkan penurunan kadar air medium (MWC), potensial air (WP) dan potensial osmotik (OP) semua spesies.  Gandum mamiliki nilai WP dan OP yang paling rendah sementara E. crusgallii memiliki nilai yang paling tinggi akibat cekaman kekeringan. Kekeringan menyebabkan penurunan laju fotosintesis pada semua spesies yang ditandai dengan penurunan qP, namun hanya kale dan A. caudatus yang mengalami peningkatan qN akibat cekaman kekeringan. Tetap stabilnya qP dari E. crusgallii pada awal cekaman mungkin berkaitan dengan mekanisme C4 yang dimiliki oleh spesies ini.  Penurunan Fv/Fm pada E. crusgallii pada periode akhir cekaman menunjukkan bahwa spesies ini mengalami fotoinhibisi disebabkan cekaman kekeringan.
Perkembangan Morfo-anatomi Bunga, Buah, dan Biji Nyamplung (Calophyllum inophyllum L), Sebagai Tanaman Penghasil Biodisel: Morpho-Anatomy Development of Flower, Fruit and Seed of Nyamplung ( Calophyllum inophyllum L) as Biodiesel-Producing Plant Hamim; Zahrul Romadlon; Dorly
Jurnal Sumberdaya Hayati Vol. 5 No. 1 (2019)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.5.1.1-10

Abstract

Nyamplung (Calophyllum inophyllum), a non-edible producing plant, is a tropical plant traditionally used by local residents as firewood, building material and medicinal plants. The research aimed to study the development of flower and fruit as well as oil content of nyamplung seeds. Two adult trees (P1 and P2) with different provenance were used in experiment. Morphology of flower and fruit, and seed germination were observed from the field, while the microscopic anatomical observations and oil content analysis were carried out in the laboratory. The result showed that a single generative bud generally had 8-15 flowers. The flower was arranged in bunches, with 4 petals, 4 sepals, single pistil and 4 files of anthers surrounded the pistil. In some cases, the flowers had 5 sepals, or 3 sepals and 3 anthers files. Fruits were round, green and turned to harden with brownish color during maturation. Flower bud grown from bud was ready to blossom 27 days after initiation (DAI). There were up to 48.52% flower buds drop at 24 DAI, and they were still increase up to 64.5% flower drop during the whole of flower development. The observation also showed that fruit drop rate of P2 plant was higher than P1. After flower bloom for 3 or 4 days after anthesis, the flowers started to lose their parts. The fruits started to ripe 8-10 week after anthesis (WAA). Seeds from P2 tree had oil content of 51.79%, which was higher than the P1 tree (42.57%).
JABON (Anthocephalus cadamba Roxb) POTENCY FOR REMEDIATING LEAD (Pb) TOXICITY UNDER NUTRIENT CULTURE CONDITION Luluk Setyaningsih; Yadi Setiadi; Sri Wilarso Budi; Hamim Hamim; Didy Sopandie
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 25 No. 1 (2018)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2018.25.1.712

Abstract

Information on metal adaptation of plants is necessary to understand the mechanism of heavy metal, including lead (Pb), remediation mediated by forest plants in contaminated land. This study aimed to find out the adaptation mechanism of jabon (Anthocephalus cadamba Roxb) seedlings to excessive lead level based on the tolerance index of growth performance and lead transport to plant tissue.  The seedlings were exposed to lead (Pb(NO3)2) with the concentrations of 0, 0.5, 1, 1.5, 5 and 10 mM in nutrient culture for 15 days. The result showed that the tolerance index (TI) of the seedlings was significantly decreased by Pb exposure up to 1.5 mM, but the TI values were more than 75%.  All seedlings died at Pb concentration of 5 mM and up.  Pb accumulated in all parts of the seedlings, with the highest concentration found in the leaves (735.9 ppm) under Pb concentration of 0.5 mM.   The Pb was found to be transported to the top portion of the seedlings indicated by Transport Factor (TF) that was more than 1. The results suggest that jabon can adapt to excessive Pb exposure up to 1.5 mM and has the potential as a remediator plant.
KARAKTERISTIK PERTUMBUHAN JARAK PAGAR (Jatropha curcas L.) YANG DITAMBAHKAN CENDAWAN ENDOFIT PADA LAHAN PASCA TAMBANG TIMAH Sukmarayu P. Gedoan; Alex Hartana; Hamim Hamim; Utut Widyastuti; Nampiah Sukarno
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol. 3 No. 1 (2013): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Graduate School Bogor Agricultural University (SPs IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.3.1.10

Abstract

Tin mining activity caused canging in physical and chemical characteristic of the soil that were not suitable for growth of plants. The objective of this experiment was to study accessions of Jatropha curcas planted on post tin mining land which were given endophyte. This research was conducted in a Sinar Baru village TS 133, district of Bangka, Bangka Belitung province for field research conducted in May 2007 to April 2008. The experimentas a factorial experiment in the design of the randomized complete block design with three replications. The main plot is 7 accessions consisting of: accession Madiun, Ponorogo, Jember, Dompu, Lampung, Bengkulu, andSukabumi, while the subplot of the land without giving endophyte fungal (control) and the provision of endophyte fungal in baglog 250 g. Each experimental unit contained four plants per plot.The result showed that vegetative growth the highest for the former tin mining land given endophyte fungal vary in some accessions. Accession to the highest Sukabumi: plant height, branch number, plant dry weight, dry weight of the shoot, and root dry weight, the largest diameter have Jember accession, accession Dompu had the highest canopy diameter, and the accession of Lampung has the lowest ratio of shoot roots.Keywords: Jatropha curcas, accession, fungal endophyte
APLIKASI PUPUK HAYATI (PLANT GROWTH PROMOTING RHIZOBACTERIA) YANG TELAH DISIMPAN TERHADAP PERTUMBUHAN TANAMAN JAGUNG Var. BISMA Nurul Hidayati; Hamim Hamim; Nisa Rachmania Mubarik
MaduRanch: Jurnal Ilmu-Ilmu Peternakan Vol 2, No 1 (2017): MaduRanch: Jurnal Ilmu-Ilmu Peternakan
Publisher : MaduRanch: Jurnal Ilmu-Ilmu Peternakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.309 KB)

Abstract

Aplikasi pupuk hayati yang mengandung mikrob hidup dapat memacu pertumbuhan tanaman. Percobaan dimaksudkan untuk menguji viabilitas Bacillus sp., Pseudomonas sp., Azospirillum sp., dan Azotobacter sp. yang terdapat pada pupuk hayati dikeringkan dengan metode kering beku pada beberapa bulan penyimpanan. Viabilitas sel bakteri diuji setelah disimpan 0, 1, 2, 4, dan 6 bulan. Pupuk hayati tersebut diaplikasi pada tanaman jagung var. Bisma untuk diamati efektivitasnya. Penelitian ini dilakukan di rumah kaca dengan menggunakan metode Rancangan Acak Lengkap (RAL) yang terdiri atas 8 perlakuan dengan 5 ulangan. Perlakuan tanah tanpa pemupukan (P1); tanah dengan pupuk NPK (P2); tanah dengan kompos (P3); tanah dengan kompos dan pupuk hayati penyimpanan 0, 1, 2, 4, dan 6 bulan (P4-P8). Hasil uji menunjukkan viabilitas bakteri sedikit menurun setelah proses kering beku (freeze drying) kemudian stabil sampai penyimpanan 2 bulan. Setelah 2 bulan, viabilitas masing-masing bakteri yang terkandung dalam pupuk hayati menurun. Aplikasi pupuk hayati penyimpanan 0, 1, 2, 4, dan 6 bulan meningkatkan pertumbuhan dan produksi tanaman jagung. Namun semakin lama penyimpanan menyebabkan efektivitas pupuk hayati untuk mendukung pertumbuhan dan produksi jagung menurun. Produksi jagung menurun dari 109.36 gram pada P5menjadi 70.46 gram pada P8.
Application of silica solubilizing bacteria increases water efficiency in maize Indah Puspita SARI; Yulin LESTARI; HAMIM HAMIM; Laksmita Prima SANTI
E-Journal Menara Perkebunan Vol 90, No 1 (2022): April, 2022
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v90i1.493

Abstract

Abstract Global climate change will result in decreased rainfall and increased evaporation. Thus, it is estimated that the frequency and severity of drought stress will get worse. Silica increases plant drought resistance by improving water use efficiency in plants. Despite its abundant availability in soil, most silica sources are not available to plants due to their low solubility. Silica solubilizing bacteria (SSB) have an important role in increasing the available silica. This study aims to observe the silica solubilizing activity of three SSB isolates collections of PPBBI on insoluble silica sources, including magnesium trisilicate, quartz, and feldspar, and see their effects on increasing water use efficiency in corn plants via drought experiments. SSB activity was measured using the modified standard method of 4500-SiO2 D Heteropoly blue. Drought control in the greenhouse follows the Snow and Tingey system. The experimental design used a completely randomized design factorial with irrigation conditions and SSB species as variables. Water use efficiency is measured in real-time with a sap flow meter. The results showed that SSB Pseudomonas fluorescens-B41 had the highest silica dissolving activity 81.93 ppm on Mg-trisilicate. The application of SSB can reduce maize transpiration rate and increase water use efficiency up to 84% under moderate drought stress and 46% under normal irrigation, but in severe drought stress, where the nutrient solution was maintained at 25 cm from plant root, water use efficiency was not significant. This is suspected due to the extreme drought conditions in the potting soil so that the applied SSB cannot maintain its activities.[Keywords: aquaporin, drought stress, Snow and Tingey system, SSB, quartz]AbstrakPerubahan iklim global mengakibatkan penurunan curah hujan dan peningkatan evaporasi, sehingga diperkirakan frekuensi dan tingkat keparahan cekaman kekeringan akan semakin tinggi. Silika (Si) diketahui dapat meningkatkan ketahanan tanaman terhadap kekeringan dengan cara memperbaiki efisiensi penggunaan air pada tanaman. Meskipun ketersediaanya berlimpah di tanah, sebagian besar Si dalam bentuk yang tidak tersedia bagi tanaman, karena sifat kelarutannya yang rendah. Untuk meningkatkan silika tersedia bagi tanaman, bakteri pelarut silika (BPS) memiliki peranan yang penting. Penelitian ini bertujuan menguji aktivitas pelarutan silika dari tiga koleksi isolat BPS Pusat Penelitian Bioteknologi dan Bioindustri Indonesia (PPBBI) pada sumber silika tidak larut berupa magnesium trisilikat, kuarsa, dan feldspar, serta melihat pengaruh aplikasi BPS terhadap efisiensi penggunaan air pada tanaman jagung yang diberi perlakuan cekaman kekeringan. Aktivitas pelarutan silika diukur menggunakan modifikasi metode standar 4500-SiO2 D Heteropoly blue. Pengendalian kekeringan di rumah kaca mengadaptasi sistem Snow dan Tingey. Rancangan percobaan menggunakan rancangan acak lengkap faktorial dengan kondisi kekeringan dan jenis bakteri BPS sebagai peubah bebas. Efisiensi penggunaan air diukur secara real time dengan sap flow meter. Hasil penelitian menunjukkan bahwa BPS dengan kode Pseudomonas fluorescens-B.41 memiliki aktivitas pelarutan silika tertinggi pada susbtrat Mg-trisilika yaitu 81,93 ppm. Aplikasi BPS menurunkan laju transpirasi jagung dan meningkatkan efisiensi penggunaan air hingga 84% pada cekaman kekeringan sedang dan 46% pada irigasi normal, namun pada cekaman kekeringan parah, dimana larutan hara dipertahankan pada jarak 25 cm dari sistem perakaran efisiensi penggunaan air tidak signifikan. Diduga hal ini disebabkan kondisi kekeringan pada media tanam terlalu ekstrim sehingga BPS yang diaplikasi tidak dapat mempertahankan aktivitasnya.[Kata kunci: aquaporin, cekaman kekeringan, sistem Snow dan Tingey, BPS, kuarsa]
Co-Authors , Triadiati ALEX HARTANA Alex Hartana Alex Hartana Aris Tri Wahyudi BEKTI KURNIAWATI Deden Saprudin Devi Media Siska Dian Hendrayanti Didy Sopandie Dorly Dorly DWI ANDREAS SANTOSA Hadisunarso Hadisunarso Hirmas Fuady Putra HIRMAS FUADY PUTRA, HIRMAS FUADY Iman Rusmana Inayatur Rosyidah Indah Puspita SARI Laksmita Prima SANTI Luluk Setyaningsih Lutfiani Lutfiani Miftahuddin Miftahudin . Mubasyiroh, Mubasyiroh Muhammad Jusuf MUNIF GHULAMAHDI Nampiah Sukarno Nani Heryani NISA RACHMANIA MUBARIK Nugroho, Robertus Nurul Hidayati Putri Nur Azizah Hutagalung Rani Apriyani Raharja Robertus Nugroho Sigit Tri Wibowo Smeer, Zeid B Sri Wilarso Budi R Suharsono Suharsono Sukmarayu P Gedoan Sukmarayu P. Gedoan Sulistyaningsih, Yohana C Tri Muji Ermayanti Tri Muji Ermayanti Turhadi Turhadi UTUT WIDYASTUTI Utut Widyastuti UTUT WIDYASTUTI Yadi Setiadi Yohana Caecilia Sulistyaningsih Yuli Siti Fatma YULIN LESTARI Zahrul Romadlon