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All Journal International Journal of Public Health Science (IJPHS) ACTA VETERINARIA INDONESIANA Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga. SOCA: Jurnal Sosial Ekonomi Pertanian Buletin Veteriner Udayana Jurnal AGROTEKNOLOGI Jurnal Ilmu Dasar Jurnal Natur Indonesia Jurnal Kedokteran Syiah Kuala Media Penelitian dan Pengembangan Kesehatan e-Journal Pustaka Kesehatan Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Indonesian Journal of Urology TEMA (Jurnal Tera Ilmu Akuntansi) JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Journal of Agromedicine and Medical Sciences Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Ners dan Kebidanan (Journal of Ners and Midwifery) Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan NurseLine Journal SRIWIJAYA JOURNAL OF MEDICINE Journal of Health Sciences JHECDs: Journal of Health Epidemiology and Communicable Diseases
ENNY SUSWATI
Department Of Microbiology, Faculty Of Medicine, University Of Jember, Jember, 68121 Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Health Professions Immunology & microbiology Library & Information Science Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Journal : e-Journal Pustaka Kesehatan

 1 
Uji In Vitro Efek Ekstrak Etanol Biji Kakao (Theobroma cacao) sebagai Antibakteri terhadap Propionibacterium acnes (In Vitro Test of the Effect of Cocoa Beans (Theobroma cacao) Ethanolic Extract as an Antibacterial against Propionibacterium acnes) Ariska Nur Aida; Enny Suswati; Misnawi Misnawi
Pustaka Kesehatan Vol 4 No 1 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Acne vulgaris is a chronic inflammatory disease of the pilosebaceous follicle, characterized by comedones, papules, pustules, cysts and nodules in certain sites of predilection, such as face, neck and back. Propionibacterium acnes are the most recognized bacteria as a key factor for the development of acne. The purpose of this research was to identify the antibacterial effect and Minimum Inhibitory Concentration (MIC) of ethanolic extract of Cocoa beans (T. cacao) against the growth of P. acnes by in vitro method. Antibacterial activity was measured by disk diffusion (Kirby Bauer) method. This was a quasi-experimental with posttest only control group design. Samples used was colony of P. acnes. Concentration of test solution used was 7.81; 15.62; 31.25; 62.50; 125; 250; 500; and 1000 mg/ml. Positive control used clindamycin suspension and negative control used sterile distilled water. By direct measurement, MIC was obtained at the dose of 31.25 mg/ml, by using linier regretion statistical analysis, the quantitatif MIC showed at the dose of 16.93 mg/ml. Analysis using ANOVA showed significance value p=0.000 (p<0.05). In conclusion, ethanolic extract of Cocoa beans had an antibacterial effect on P. acnes by in vitro method.   Keywords: antibacterial effect, cocoa beans, Propionibacterium acnes
Efek Ekstrak Etanol Biji Kakao (Theobroma cacao) sebagai Antibakteri terhadap Pseudomonas aeruginosa secara In Vitro (The Effect of Ethanolic Extract of Cocoa Beans (Theobroma cacao) as an Antibacterial against Pseudomonas Devi Chintya Kumalasari; Enny Suswati; Misnawi Misnawi
Pustaka Kesehatan Vol 3 No 1 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Nosocomial infection is one of the most serious healthy problem in the world. It causes by Pseudomonas aeruginosa which has a high resistance to antibiotic. One alternative to overcome this problem is the utilization of chocolate (Theobroma cacao) because it contains polifenol that can be used as an antibacterial agent. The aim of this research was to determine antibacterial effect and Minimum Inhibitory Concentration (MIC) of ethanolic extract of cocoa beans to P. aeruginosa by in vitro method. Antibacterial activity test used disk diffusion (Kirby Bauer) method. This type of research was a quasi-experimental with posttest only control group design. Samples used was colony of P. aeruginosa. Concentrations of test solution used were 7,81; 15,62; 31,25; 62,50; 125; 250; 500; and 1000 mg / ml. Positive control used cefepime suspension and negative control used sterile distilled water. The result showed that inhibition zones formed at a concentration of 7,81 mg/ml to 1000 mg/ml. The higher the concentration of the cocoa beans extract, the wider the inhibition zones formed. By direct measurement, MIC was obtained at the dose of 7.81 mg / ml while statistical analysis yielded an MIC value of more than 6.95 mg / ml. In conclusion, the ethanolic extract of cocoa beans had antibacterial effect on P. aeruginosa in vitro.   Keywords: antibacterial effect, cocoa beans, Pseudomonas aeruginosa
Uji in vitro Aktivitas Antibakteri Minyak Atsiri Batang Sereh (Cymbopogon citratus) terhadap Shigella dysenteriae Yunita Wulansari; Enny Suswati; Septa Surya Wahyudi
Pustaka Kesehatan Vol 6 No 2 (2018)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v6i2.7665

Abstract

Shigella dysenteriae is bacteria that caused diarrhea with high morbidity and mortality. Resistance and toxicity has reported in some antibiotics therapy towards S. dysentriae. Therefore, alternative therapy based on herbal plants is needed. Chymbopogon citratus oil can be used as an antibacterial to Escherichia coli, Klebsiella pneumoniae, Streptococcus mutans, all strains Staphlylococcus sp, Bacillus cereus and Bacillus subtilis. This study aimed to investigate antibacterial activity of C. citratus oils on the growth of S. dysentriae and its minimum inhibitory concentration (MIC). This type of research was a quasi experimental. Antibacterial activity test performed by diffusion method. Subjects in this study were S. dysentriae. C. citratus oil concentration used were 15 μl/ml, 20 μl/ml, 25 μl/ml, 30 μl/ml, 35 μl/ml and 40 μl/ml. Positive control group was given ciprofloxacin 5 μl/ml and a negative control group was given tween-80. Inhibition zone was observed at a concentration of 25 µl/ml (P3). The MIC of C. citratus oils againts S. dysentriae was at a concentration of 25 µl/ml. Kruskal Wallis analysis showed that p=0.000 and α=0.05, it was revealed that there were differences between antibacterial activity amoung tested group with different concentration. Keywords: Shigella dysenteriae, Chymbopogon citratus oil, antibacterial
Pengaruh Pemberian Jus Semangka terhadap Kelelahan Otot dan Delayed Onset Muscle Soreness setelah Latihan Beban (The effect of Watermelon Juice on Muscle Fatigue and Delayed Onset Muscle Soreness after Weight Training) Prayoga Adinawer Sirait; Cholis Abrori; Enny Suswati
Pustaka Kesehatan Vol 4 No 1 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

The main problem that is often faced by athlete is fatigue. It can decrease maximum muscle strength. To overcome this problem, many athletes use ergogenic aids such as watermelon. The purpose of this study was to determine the effect of watermelon juice on reduction of muscle fatigue and delayed onset muscle soreness (DOMS) after weight training. Muscle fatigue was measured by maximum repetition done by the subjects in the last set and DOMS was measured by post-training muscle soreness intensity. By using a randomized crossover design, 20 subjects were recruited then divided into 2 groups, a treatment group and a control group. The subjects were asked to do a training protocol to induce muscle fatigue and DOMS. As much as 500 ml watermelon juice was consumed in 1 of 2 training sessions and a placebo was consumed in the other. The number of repetitions showed a significant increase on treatment group than control group (p<0,05). Passive muscle soreness intensity and active muscle soreness intensity showed a significant decrease on 24 hours and 48 hours post-training after administration of watermelon juice (p=0,000). We conclude that there was an effect of watermelon juice on muscle fatigue and DOMS after weight training.   Keywords: weight training, muscle fatigue, DOMS, watermelon, citrulline
Aktivitas Ekstrak Metanol Bawang Putih (Allium sativum) sebagai Penghambat Pembentukan Biofilm pada Pseudomonas aeruginosa (The Activity of Methanolic Extract of Garlic (Allium sativum) in Inhibiting Growth of Biofilm in Pseudomonas aeruginosa) Risty Pradana Linggan Wangi; Enny Suswati; Desie Dwi Wisudanti
Pustaka Kesehatan Vol 5 No 3 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Garlic (Allium sativum) is one of the most important agricultural product that has organosulphur compound, such as alliin, allicin, and ajoene. Those organosulphur compound used as antibiofilm agent by inhibiting adhesion and quorum sensing process in Pseudomonas aeruginosa. The purpose of study was to identify the antibiofilm effect and Minimum Biofilm Inhibitory Concentration (MBIC) of methanolic extract of garlic against the growth of P. aeruginosa biofilm. This was quasi experimental with posttest only control group design. The colony of P. aeruginosa were divided into 7 groups with 5 groups used methanolic extract of garlic as P1-P5 at a dose 0,3; 0,6; 0,9;1,2; and 1,5 mg/mL, positive control group (K+) used NAC 80 mg/mL, and negative control group (K-) used sterile water. This research using Microtitter Plate Biofilm Assay Method with 4 replication. The absorbance value was analized by using One Way Anova statistical analysis, it showed the significance value p=0,000 (p<0,05). By using linier regression, the MBIC was obtained at the dose of 0,3468 mg/mL. In conclusion, methanolic extract of garlic had ability to inhibit the growth biofilm of P. aeruginosa. Keywords: biofilm, garlic, Minimum Biofilm Inhibitory Concentration, P. aeruginosa
Respon Imunogenitas Antibodi Poliklonal IgY terhadap Protein Adhesi Pili 95 kDa Shigella dysenteriae asihanti rosita ferdiana; Enny Suswati; Kristianningrum Dian Sofiana
Pustaka Kesehatan Vol 2 No 2 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Shigella dysenteriae is the most frequently cause of deaths of dysentery cases in infants and toddlers. Shigella dysenteriae has a pili that act as an adhesin molecule and will detect in human body as antigens which is involved in producing antibodies. This study was conducted to prove that Shigella dysenteriae pili adhesion protein 95 kDa is immunogenic. This was an experimental laboratory study with quasy experimental design in vitro. The study began with mice erythrocyte cell isolation, IgY polyclonal antibodies isolation, mice intestinal enterocytes cell isolation, hemagglutination inhibition test, and adhesion inhibition test. The haemagglutination inhibition test showed that the pili adhesion protein 95 kDa of S. dysenteriae able to inhibit the haemagglutination of erythrocytes mice Balb/C up to ½ dilution. While, the adhesion inhibition test revealed that the fewer of antibody concentration on enterocytes, the greater bacteria attached. According to the linear regression test results, the index adhesion value increased along with the decreased of antibody concentration. In conclusion, pili adhesion protein 95 kDa from S. dysenteriae was immunogenic and could inhibit the hemagglutination and adhesion between S. dysenteriae and mice enterocytes cells.   Keywords: immunogenicity, IgY polyclonal antibody, Shigella dysenteriae, pili adhesion protein.
Co-Authors Abu Khoiri Aditya Oktavianto Ahmad Hasbi Al-Muzaky Ahmad Hasbi Al-Muzaky Alvien Zahrotun Nadhiva Angga M Raharjo Angga Mardro Raharjo Anisa Rizca Putri APRI KUNTARININGSIH Arifah Nur Hasanah Ariska Nur Aida asihanti rosita ferdiana Azham Purwandhono Bagus Hermansyah Bagus Hermansyah Bagus Hermansyah Bagus Satrio Pambudi Bagus Satrio Pambudi, Bagus Satrio Bima Setia Sandya Nugraha Cholis Abrori, Cholis Desie D. Wisudanti Desie Dwi Wisudanti, Desie Dwi Devi Chintya Kumalasari Dian H. Purnamasari Diana C Diana C Mufida Diana C. Mufida Diana Chusna Mufida Dini Agustina Dwita Aryadina Dwita Aryadina Rachmawati Elly N Sakinah Elly Nurus Sakinah, Elly Nurus Elvia Rahmi Marga Putri Erfan Efendi Habib Mustofa Hanifah S Haq I Ketut Sudiana Ika Rahmawati Sutejo Indra Samudra Rahmat Indra Samudra Rahmat Jauhar Firdaus Joko Mariyono Kirana Nadyatara Kristianningrum Dian Sofiana Lailatis Shofia Laksmi Indreswari Laylatul Fitriyah Mukarromah M. Ali Shodikin Misnawi Misnawi Misnawi Misnawi Mufida Mufida Muhammad A. Shodikin Muhammad Alif Taryafi Nexia Nevarachell Onny Amirsyah Nindya Shinta Rumastika Nisrina Salsabila Firmansyah Nita Alfianti NUR K. AGUSTIN Nurhayati Nurhayati Prayoga Adinawer Sirait Probosutiksna, Nuno Febrian Probosutiksna, Nuno Febrian Putu Ayu Laksmi Lestari Regina Finka Risty Pradana Linggan Wangi Salsabila, Yuna Annisa Saskia Mediawati septa surya wahyudi, septa surya Shofia, Lailatis Siti Ananda Hardita Syahputri Supangat Supangat Supangat Supangat Suryono Suryono Ulfa Elfiah Wahyuning Ati Ashari Wiwien Sugih Utami Wiwin Sugih Utami Yudha Nurdian Yudha Nurdian Yuna Annisa Salsabila Yunita Armiyanti Yunita Armiyanti Yunita Wulansari