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All Journal International Journal of Public Health Science (IJPHS) ACTA VETERINARIA INDONESIANA Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga. SOCA: Jurnal Sosial Ekonomi Pertanian Buletin Veteriner Udayana Jurnal AGROTEKNOLOGI Jurnal Ilmu Dasar Jurnal Natur Indonesia Jurnal Kedokteran Syiah Kuala Media Penelitian dan Pengembangan Kesehatan e-Journal Pustaka Kesehatan Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Indonesian Journal of Urology TEMA (Jurnal Tera Ilmu Akuntansi) JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Journal of Agromedicine and Medical Sciences Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Ners dan Kebidanan (Journal of Ners and Midwifery) Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan NurseLine Journal SRIWIJAYA JOURNAL OF MEDICINE Journal of Health Sciences JHECDs: Journal of Health Epidemiology and Communicable Diseases
ENNY SUSWATI
Department Of Microbiology, Faculty Of Medicine, University Of Jember, Jember, 68121 Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Health Professions Immunology & microbiology Library & Information Science Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Journal : Journal of Agromedicine and Medical Sciences

 1 
The Effect of Mirabilis jalapa Leaf Ethanolic Extract against Streptococcus pyogenes Pambudi, Bagus Satrio; Suswati, Enny; Firdaus, Jauhar
Journal of Agromedicine and Medical Sciences Vol 2, No 1 (2016)
Publisher : Medical Faculty of Jember University (Fakultas Kedokteran Universitas Jember)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Streptococcus pyogenes is a Gram positive bacteria that commonly cause disease in human. If not treated immediately, this bacteria can cause serious complication such as reumatic fever that causing heart valve tissue damage. Penicilin, drug of choice to eradicate S. pyogenes, oftenly cause various side effects such as anaphylaxis. This study aimed to investigate the effect of Mirabilis jalapa leaf ethanolic extract against S. pyogenes growth using in vitro techniques. The study design was a quasi experimental design. S. pyogenes culture as the study subject were divided into positive control group (penicilin V 100 IU), negative control group (NaCMC 0,5%), and eight treatment groups that were given with M. jalapa leaf ethanolic extract as much as 0,1 mg/ml, 1 mg/ml, 5 mg/ml, 10 mg/ml, 20 mg/ml, 30 mg/ml, 40mg/ml, and 50 mg/ml. After 24 hours incubation periods, the inhibition zone were found in all treatment group except in concentration 0,1 mg/ml. This study showed that M. jalapa leaf ethanolic extract could inhibit the growth of S. pyogenes. There was significant correlation between the concentration of M. jalapa leaf ethanolic extract and the diameter of inhibition zone (p=0,00), the higher concentration of M. jalapa leaf extract, the larger diameter of inhibition zone of S. pyogenes. Keywords: Mirabilis jalapa, leaf extract, antimicrobial activity
The Role of Pili Protein 38,6 kDa Klebsiella pneumoniae as a Hemagglutinin and Adhesin Protein which Serves as a Virulence Factor Regina Finka; Dini Agustina; Dwita Aryadina Rachmawati; Enny Suswati; Diana Chusna Mufida; Ali Shodikin
Journal of Agromedicine and Medical Sciences Vol 5 No 2 (2019)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v5i2.9558

Abstract

Klebsiella pneumoniae is an infectious bacteria in various parts of the body. The presence of proteins in pili that will bind to cell surface receptors (adhesin proteins) and cell membrane sugar molecules (hemagglutinin proteins) is a very influential factor in the ability of K. pneumoniae to enter the human body. The purpose of this study was to determine the role of pili 38.6 kDa K. pneumoniae protein as a hemagglutinin and adhesin protein which functions as a virulence factor. This type of research is pure experimental research using the electrophoresis method (SDS-PAGE) to obtain protein. The protein obtained was then tested for hemagglutination and adhesion test using erythrocyte cells and enterocytes of BALB / C mice to determine their role as hemagglutinin and adhesin proteins. Conclusion of this study pili protein 38.6 kDa Klebsiella pneumoniae acts as a hemagglutinin and adhesion protein which functions as a virulence factor. Keywords: Klebsiella pneumonia, 38,6 kDa Protein, Hemagglutinin, Adhesin
Tetraciclyne Resistance Eschericia coli Isolated From Broiler Chicken Meat Anisa Rizca Putri; Enny Suswati; Laksmi Indreswari
Journal of Agromedicine and Medical Sciences Vol 4 No 1 (2018)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v4i1.6402

Abstract

Antibiotic resistance is a serious health problem that have ben uneffective therapy. The cause of antibiotic resistance 80% comes from food of animal origin such as Broiler Chicken. E. coli contamination in antibiotic resistant chicken has been shown to transfer genetic factors between bacteria in the human intestinal system. Almost all broiler breeders use commercial feed containing tetracycline antibiotics. The use of antibiotics in the feed mixture is one of the risk factors for resistance. The aim of this study was to get an information of antibiotic resistance E. coli which isolated from broiler meat. Identification test of E. coli used two phase, presumtive test and confirmed test. Sensitivity test for E. coli to antibiotic by disc diffusion Kirby Bauer method. This study used 6 sample of upper thigh broiler meat, the antibiotic use tetracycline. The data were analyzed descriptively. The results of this study show that from 6 isolate sample, there are 4 isolate identificated as E. coli and 2 isolate show resistance to the tetracycline antibiotic. The conclusion of this study showed resistance has occurred on 50% sample.
Difference of Right Ventricular Systolic Function Before and After Hemodialysis in Chronic Kidney Disease Stage V In RSD dr. Soebandi Jember Saskia Mediawati; Suryono Suryono; Enny Suswati
Journal of Agromedicine and Medical Sciences Vol 4 No 2 (2018)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v4i2.7873

Abstract

End stage renal disease is a clinical state with an irreversible chronic decreasing function of renal that need a hemodialysis or renal transplant as theraphy. One of the complication of End stage renal disease is cardiovascular. Cardiovascular complication is a major cause of mortality and mobility in End stage renal disease by 44% in Indonesia. Systolic function of right ventricle can be used to assess the heart function. The purpose of this study is to differentiate the systolic function of right ventricle in patient with end stage renal disease in RSD dr. Soebandi Jember. We used analytical observational approach with cross sectional method held at Dialysis and Echocardiography Unit of RSD dr. Soebandi Jember from September to November 2017. Thirty patients with end stage renal disease who routinely undergoes dialysis were observed. Shapiro-Wilk test results 0,000 significance shows that the data is not normally distribute. Wilcoxon test results significance level 0,005 which shows there is a differentiation between before and after hemodialysis of systolic function in end stage renal disease patients of RSD dr. Soebandi Jember. Keywords : End stage renal disease, systolic function on right ventricle, hemodialysis
The Effectiveness of Edamame Seed (Glycine max L. Merril) Ethanolic Extract to Fibroblast Count on Second Degree Burn Wound Healing Arifah Nur Hasanah; Ika Rahmawati Sutejo; Enny Suswati
Journal of Agromedicine and Medical Sciences Vol 5 No 3 (2019)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v5i3.6831

Abstract

Burn wound healing process consists of three phases of inflammation, proliferation and remodelling. The number of fibroblasts has increased during the proliferation phase. Oxidative stress in burn may disrupt proliferation through TGF-β inhibition, result in decreased number of fibroblasts and poor burn wound healing. Genistein in edamame seeds has anti-inflammatory, antibacterial and antioxidant properties. Edamame contains vitamins A, C, and E which is also have antioxidant properties. Edamame has been suggested to protect burn wound healing from oxidative stress. This study aimed to understand the effectiveness of edamame seed (Glycine max L. Merril) ethanolic extract (ED) to fibroblast count in second degree burn wound healing. This is true experimental research with post test only control group design. The study was conducted at University of Jember during November until December 2017. The sample of this research was 24 rat models of second degree burn which was made by attaching hot metal (60 ° C) for 5 seconds on the shaved back of the rats. The sample was divided into 6 groups with topical treatment, among others: positive control group (silver sulfadiazine), negative control group (Na CMC 0.5%), group with ED 20%, ED 40%, ED 60%, and ED 80%. Treatment was given for 15 days and skin sample was taken on the 16th day. Observation of the number of fibroblasts was done by taking pictures of histhologic preparations in Hematoxylin and Eosin staining under 400x magnification of light microscope. The number of fibroblast of each picture was processed using Image-J software. In one way ANOVA test, significance value of 0.011 (p≤0,05) indicated that there was at least a significant difference between 2 groups. LSD’s post hoc analysis showed that there was a significant difference in the group ED 20, 40, and 60% compared with negative control. Keyword: burn wound healing, vegetable soybean, genistein, fibroblast count, silver sulfadiazine
The Effect of Mirabilis jalapa Leaf Ethanolic Extract against Streptococcus pyogenes Bagus Satrio Pambudi; Enny Suswati; Jauhar Firdaus
Journal of Agromedicine and Medical Sciences Vol 2 No 1 (2016)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Streptococcus pyogenes is a Gram positive bacteria that commonly cause disease in human. If not treated immediately, this bacteria can cause serious complication such as reumatic fever that causing heart valve tissue damage. Penicilin, drug of choice to eradicate S. pyogenes, oftenly cause various side effects such as anaphylaxis. This study aimed to investigate the effect of Mirabilis jalapa leaf ethanolic extract against S. pyogenes growth using in vitro techniques. The study design was a quasi experimental design. S. pyogenes culture as the study subject were divided into positive control group (penicilin V 100 IU), negative control group (NaCMC 0,5%), and eight treatment groups that were given with M. jalapa leaf ethanolic extract as much as 0,1 mg/ml, 1 mg/ml, 5 mg/ml, 10 mg/ml, 20 mg/ml, 30 mg/ml, 40mg/ml, and 50 mg/ml. After 24 hours incubation periods, the inhibition zone were found in all treatment group except in concentration 0,1 mg/ml. This study showed that M. jalapa leaf ethanolic extract could inhibit the growth of S. pyogenes. There was significant correlation between the concentration of M. jalapa leaf ethanolic extract and the diameter of inhibition zone (p=0,00), the higher concentration of M. jalapa leaf extract, the larger diameter of inhibition zone of S. pyogenes. Keywords: Mirabilis jalapa, leaf extract, antimicrobial activity
Co-Authors Abu Khoiri Aditya Oktavianto Ahmad Hasbi Al-Muzaky Ahmad Hasbi Al-Muzaky Alvien Zahrotun Nadhiva Angga M Raharjo Angga Mardro Raharjo Anisa Rizca Putri APRI KUNTARININGSIH Arifah Nur Hasanah Ariska Nur Aida asihanti rosita ferdiana Azham Purwandhono Bagus Hermansyah Bagus Hermansyah Bagus Hermansyah Bagus Satrio Pambudi Bagus Satrio Pambudi, Bagus Satrio Bima Setia Sandya Nugraha Cholis Abrori, Cholis Desie D. Wisudanti Desie Dwi Wisudanti, Desie Dwi Devi Chintya Kumalasari Dian H. Purnamasari Diana C Diana C Mufida Diana C. Mufida Diana Chusna Mufida Dini Agustina Dwita Aryadina Dwita Aryadina Rachmawati Elly N Sakinah Elly Nurus Sakinah, Elly Nurus Elvia Rahmi Marga Putri Erfan Efendi Habib Mustofa Hanifah S Haq I Ketut Sudiana Ika Rahmawati Sutejo Indra Samudra Rahmat Indra Samudra Rahmat Jauhar Firdaus Joko Mariyono Kirana Nadyatara Kristianningrum Dian Sofiana Lailatis Shofia Laksmi Indreswari Laylatul Fitriyah Mukarromah M. Ali Shodikin Misnawi Misnawi Misnawi Misnawi Mufida Mufida Muhammad A. Shodikin Muhammad Alif Taryafi Nexia Nevarachell Onny Amirsyah Nindya Shinta Rumastika Nisrina Salsabila Firmansyah Nita Alfianti NUR K. AGUSTIN Nurhayati Nurhayati Prayoga Adinawer Sirait Probosutiksna, Nuno Febrian Probosutiksna, Nuno Febrian Putu Ayu Laksmi Lestari Regina Finka Risty Pradana Linggan Wangi Salsabila, Yuna Annisa Saskia Mediawati septa surya wahyudi, septa surya Shofia, Lailatis Siti Ananda Hardita Syahputri Supangat Supangat Supangat Supangat Suryono Suryono Ulfa Elfiah Wahyuning Ati Ashari Wiwien Sugih Utami Wiwin Sugih Utami Yudha Nurdian Yudha Nurdian Yuna Annisa Salsabila Yunita Armiyanti Yunita Armiyanti Yunita Wulansari