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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Jurnal Ilmu Ternak dan Veteriner Indonesian Journal of Biotechnology Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Jurnal Kedokteran Hewan
Tri Untari
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary Other

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Journal : Jurnal Veteriner

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Isolasi, Identifikasi, Sifat Fisik, dan Biologi Virus Tetelo yang Diisolasi dari Kasus di Lapangan (ISOLATION, IDENTIFICATION, PHISICAL, AND BIOLOGICAL CHARACTER OF NEWCASTLE DISEASE VIRUS ISOLATED FROM FIELD CASES) Michael Haryadi Wibowo; Tri Untari; Anastasia Endang Tri Hastuti Wahyuni
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

native chicken farm suspected to Newcastle disease (ND) virus infection. Specimens were taken andcollected from the lung was further processed. Suspected materials were inoculated into allantoic sacc inspecific pathogenic free of 10 days embryonating egg chicken. The growth of the virus was determined withthe ability to agglutinate the chicken red blood cells or hemaglutination test. Positive hemaglutinationwas performed with hemaglutinatin inhibition test using specific antibody against ND virus. Method forND virus isolation, propagation and identification were based on the standard procedure of serologicalidentification for ND virus serological identification. 13 out of 34 samples were identified as ND viruses.Observation on the course and time of the virus to kill the chicken embryo could be differentiated intomoderate virus patho-type were 10 isolates and a virulent strains were 3 isolates. Further characterizationbased on the elution time observation indicated 11 isolates were not pathogenic strain and 2 isolates werenot virulent strain. Hemagglutinin stability study revealed that 11 isolates were sensitive being heated at560C for 30 minutes while 2 isolates were resistant. Biological characteristic of ND virus to hemagglutinateon various mammalian red blood cells indicating that most isolates were HA negative. Two isolates wereHA positive with cattle, horse and sheep red blood cell, and one isolate indicated positive HA test by usingsheep red blood cell. Control virus was lentogenic patho-type of La Sota strain showed HA and HI testpositive, elution time was 29 minutes, stability on the hemagglutinin after heating was 2 minutes and HApositive with cattle, horse and sheep red blood cell.
Deteksi Bovine Herpesvirus-1 Secara Immunohistokimia pada Membran Korioallantois Telur Ayam Berembrio (IMMUNOHISTOCHEMISTRY DETECTION OF BOVINE HERPESVIRUS-1 IN CORIOALLANTOIC MEMBRANE OF CHICKEN EMBRYONATED EGG) Yuli Purwandari Kristianingrum; Charles Rangga Tabbu; Bambang Sutrisno; Sitarina Widyarini; Kurniasih .; Tri Untari; Asmarani Kusumawati
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Infectious Bovine Rhinotracheitis (IBR) is caused by Bovine Herpes virus-1 in the cattle. The clinicalsigns demonstrate depression, anorexia, swelling of the vulva, redness of the vestibule, pustule and ulceron the vaginal mucosal. Based on previous research, IBR virus from the nasal swab could be grown inchorio-allantoic membrane of embryonated chicken eggs. This study aim was to confirm whether IBR virusin cattle could be grown in embryonated chicken eggs as a substitute for cell culture. A total of five nasalswab samples from the cows that were positive for IBR infection (diagnosed by Polymerase Chain Reactionand cell culture) were inoculated on the chorio-allantois membrane of embryonated chicken eggs.Observation of lesions performed at 3-5 days after inoculation. Re-inoculation (passage) was done threetimes. Pock characteristic lesions were observed on the corioallantoic membrane with the size of 5-7 mm,rounded shape, opaque edge, with necrosis in the central area. Furthermore, pock lesions were processedfor hematoxylin and eosin staining and immuno-histochemistry. The result of hematoxylin and eosinstaining showed that the formation of intranuclear inclusion bodies and vacuolization of the epithelial cellof membrane was observed. Immuno-histochemistry staining showed positive reaction for antibodiesagainst BHV-1 in the epithelial cells membrane. In conclusion, embryonated chicken eggs could be usedas a medium for detection of IBR.
Pelacakan Gen Env-TM Virus Penyakit Jembrana Galur Tabanan 1995 dengan Metode Nucleic Acid Sequence Based Amplificaton Asmarani Kusumawati; Atik Ratnawati; Ida Arlita Wulandari; Sri Hartati; Tri Untari
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Jembrana disease is an infectious disease in Bali cattle cause by a member of lentivirus calledjembrana disease virus (JDV). It causes an acute and severe disease syndrome with short incubationperiod. As the disease has spread to several areas in Indonesia, a simple and rapid detection method isrequired. The objective of this study to apply rapid diagnostic method for JDVTabanan 1995 strain basedon Nucleic Acid Sequence Based Amplification (NASBA) methods targeting env-tm gene. The steps of thisresearch consisted of viral RNA isolation from organ and blood of cattle experimentaly infected withJDVTabanan 1995 strain . RNA amplification was conducted by NASBA using waterbath. The NASBAproducts were then separated on 2 % agarose gel. Using this technique JDV positive result was obtainedfrom organ samples such as spleen, liver, lung, prefemoralis lymph node, prescapularis lymph node andblood generating a RNA fragment of 207 bp. In this study, diagnosis method for env tm of JDV Tabanan1995 strain can be conducted by isothermal amplification NASBA.
Resistansi Antibiotik Bakteri dari Ulas Kloaka Burung Puyuh Sehat Maria Anggita; Widya Asmara; Tri Untari; Michael Haryadi Wibowo; Sidna Artanto; Okti Herawati; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Veteriner Vol 22 No 4 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.172 KB) | DOI: 10.19087/jveteriner.2021.22.4.508

Abstract

Anti Mikrob Resistan (AMR) menjadi masalah utama baik pada manusia, hewan, dan lingkungan. Pada umumnya pemberian antibiotik dilakukan oleh peternak unggas di Indonesia melalui pakan sebagai antibiotik pemacu pertumbuhan/Antibiotic Growth Promoters (AGP). Penggunaan antibiotik yang berlebihan di industri peternakan dianggap berkontribusi terhadap meningkatnya resistansi obat pada manusia. Penelitian ini bertujuan untuk mengetahui kejadian resistansi bakteri dari ulas/swab kloaka burung puyuh sehat. Sebanyak sepuluh ekor puyuh sehat dari peternakan puyuh di daerah Kalasan, Klaten, Yogyakarta diswab kloaka dan dikultur pada media cair Brain Heart Infusion (BHI). Biakan ditanam pada media Mueller Hinton Agar (MHA) dan diletakkan Sembilan jenis cakram/disk antibiotik: streptomisin, doksisiklin, fosfomisin, kloramfenikol, kolistin, siprofloksasin, ampisilin, eritromisin, dan penisilin. Setelah inkubasi pada suhu 37°C selama 18-24 jam, zona hambat yang terbentuk kemudian diukur dan ditentukan sifat resistansi dibandingkan dengan standar. Hasil menunjukkan sebanyak 20%kultur bakteri resistan terhadap streptomisin, 40% resistan terhadap doksisiklin, 40% resistan terhadap kloramfenikol, 50% resistan terhadap kolistin, 20% resistan terhadap siprofloksasin, 20% resistan terhadap ampisilin, 90% resistan terhadap eritromisin, 50% resistan terhadap penisilin, dan tidak ada resistansi terhadap fosfomisin. Terdapat satu dari sepuluh puyuh (P10) yang memiliki resistansi terhadap tujuh dari sembilan jenis antibiotik (78%) yang diujikan, dan dua dari sepuluh puyuh (P2 dan P4) memiliki resistansi terhadap dua dari sembilan antibiotik (11%). Hasil penelitian menunjukkan bahwa bakteri dari swab kloaka pada burung puyuh sehat umur 21 hari dari satu peternakan yang sama memiliki tingkat resistansi yang berbeda-beda. Sifat resistansi terhadap antibiotik dari masing-masing puyuh juga berbeda-beda.
Co-Authors Adiana Mutamsari Witaningrum AETH. Wahyuni Agnesia Endang Tri Hastuti Wahyuni Agustina Dwi Wijayanti Agustina Dwi Wijayanti Ahmad Sofyan Anastasia Endang Tri Hastuti Wahyuni Andriani Dwi Hapsari Antasiswa W. Rosetyadewi Antasiswa Windraningtyas Rosetyadewi Arfian Rahma Shanti Asmarani Kusumawati Asmarani Kusumawati Atik Ratnawati Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bambang Sutrisno Bambang Sutrisno Charles Rangga Tabbu Dian Ayu Permatasari Dian Ayu Permatasari Ema Damayanti Guntari Titik Mulyani Hamdu Hamjaya Putra Hardi Julendra Hary Purnamaningsih Haryadi M. Wibowo Haryadi Wibowo Heru Susetya Ida Arlita Wulandari Ida Fitriana Khrisdiana Putri Krisdiana Putri Kurniasih . Kurniasih Kurniasih Laksono Trisnantoro Liza Angeliya Maria Anggita Marla Anggita Michael Haryadi Wibowo Mustofa Helmi Effendi Nyoman Reishita Andriyani Okti Herawati Sidna Artanto Sidna Artanto Sidna Artanto Sitarina Widyarini Slamet Rahardjo Soedarmanto Indarjulianto Sri Hartati Sri Hartati Surya Amanu Surya Amanu Wibisono, Freshinta Jellia Widya Asmara Widya Asmara Yanuartono . Yuli Purwandari Kristiangingrum