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Sri Agus Sudjarwo
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Department of Basic Medical Veterinary of Faculty of Veterinary Medicine, Universitas Airlangga Kampus C, Mulyorejo, Kec. Mulyorejo, Kota SBY, Jawa Timur 60115 Phone: (031) 5992785 ext. 5993016
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Kota surabaya,
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INDONESIA
journal of Basic Medical Veterinary
Published by Universitas Airlangga
ISSN : 23026820     EISSN : 27749959     DOI : https://dx.doi.org/10.20473
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary
This journal published original articles, review articles, and case studies in Indonesian or English, in the scope of JBMV has a broad coverage of relevant topics across veterinary basic medical sciences which includes: preclinical and paraclinical disciplines like Anatomy, Physiology, Biochemistry, Pathology, Microbiology, Pharmacology and Medical Education.
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 57 Documents
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Effect of Aflatoxin Contaminated Feed for 40 Days on Anatomy and Histopathology of the Thymus of Laying Hens in the Starter Phase Rachella Christy; Soeharsono Soeharsono; Ismudiono Ismudiono; Hana Eliyani; Sarmanu Sarmanu; Hani Plumeriastuti
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28679

Abstract

The purpose of this study was to determine the effect of aflatoxin contaminated feed given for 40 days on the anatomy and histopathology of the thymus of laying hens in the starter phase. A total of 36 DOC laying hens were divided into two groups (P0 and P1). P0 (control group) was given 100% basal starter feed CP 521 and P1 (treatment group) was given 80% basal feed CP 524 plus 20% aflatoxin contaminated feed. Feeding contaminated with aflatoxin was carried out for 40 days, starting on the 20th to the 60th day after the DOC of laying hens was adapted for 20 days. The time of surgery in this study was carried out on the 20th, 40th, and 60th days, where at each surgery time the thymus organ was taken to collect weight data and histopathological preparations were made. The thymus weights based on time showed a significant decrease, but the intergroup thymus weights did not show a significant decrease. The decrease in thymus weight is caused by the involution process. Microscopic observations based on increased cell debris and congestion showed that P0 did not show a significant difference, but P1 showed a significant difference (p>0.05) on the 40th and 60th days. The conclusion in this study was that as much as 20% of feed contaminated with aflatoxins had not affected changes in thymus weight but had shown damage to the histopathological structure of the thymus.
Potensi Dedak Padi yang Difermentasi dengan Enzim Fitase dalam Pakan Basal Terhadap Kolesterol Total Darah Ayam Petelur Yulia Nurfitri; Mirni Lamid; Margareth Gandul Atik Yuliani; Mohamad Anam Al-Arief; Retno Sri Wahyuni; Eduardus Bimo Aksono
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28588

Abstract

This study aimed to evaluate the potential of rice bran fermented by the enzyme phytase in basal feed to the total blood cholesterol level of laying hen. This research use an experimental method in vivo with a completely randomized design consisting of six treatments and four replications in each treatment. The experimental animals used 24 48-week-old female laying hen strain Isa Brown. The treatment wa given basal feed substituted with different percentage of fermented rice bran by phytase enzyme and 2% lemuru fish oil supplementation. In this research the amount of fermented rice bran by phytase enzyme substituted on basal feed was: treatment P0 (-) (0%), P0 (+) (0%), P1 (5%), P2 (10%) P3 (15%) and P4 (20%). The results of the statistical analysis showed that there are no significant differences (p> 0.05) on total blood cholesterol levels in all treatments. The conclusion of the research is that rice bran fermented by phytase enzymes tends to decrease total blood cholesterol levels in laying hen.
The Prevalence of Domesticated Geriatri Cats in Surabaya City at April-May 2019 Rr Indah Nur Rahmawati; Nusdianto Triakoso; Anwar Ma’ruf
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28680

Abstract

This study is beneficial to give information about population of domesticated geriatric cats in Surabaya period from April to May 2019. This study is also beneficial to give information about likely risks of domesticated geriatric cats in Surabaya City based on gender, cat breed, and cat’s disease. The design of this study was Observational Retrospective using cross-sectional method. Sampling used stratified random sampling method. This study collected primary data and was analyzed descriptively using prevalence test and relative risks. That domesticated geriatric cats in Surabaya City period from April-May 2019, the oldest cats were written 17 years for male cats and 9 years for female cats. The prevalence of domesticated geriatric cats in Surabaya City period from April-May 2019 was 9 (7,5%). Geriatric cats distributed by the gender were 5 (4,2%) male and 4 (3,3%) female, where geriatric cats distributed by the cat breed were 5 (4,2%) pure breed, 4 (3,3%) mixed breed, and 0 (0%) domestic sample. The result of geriatric cats analysis based on gender showed that male cats had a change of 1,4 times higher compared to the female to reach geriatric, whereas based on cat breed it showed pure breed had a change 3,3 times compared to mixed breed to reach geriatric. On this study, the disease often appeared in domesticated geriatric cats in Surabaya City period from April -May 2019 were: on skin, respiration, digestion, and eyes. Based on relative risk analysis on geriatric cats, it had a change of gastrointetinal tract that was higher compared to other diseases.
Total Plate dan Total Staphylococcus aureus pada Daging Di Pasar Tradisional Kecamatan Mulyorejo Surabaya Nonie Olivia Adia Mutiarasari; Nenny Harijani; Fedik Abdul Rantam; Dadik Raharjo; Agnes Theresia Soelih Estoepangestie; Didik Handijatno
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28584

Abstract

This study aimed to evaluate the Total Plate Count and total Staphylococcus aureus count of beef sold in wet markets in Mulyorejo sub-district below the National Standard Indonesia (SNI 7388:2009) about maximum limit of microbial contamination in food or not. Total of twenty four samples of beef purchased from traditional markets of Tempurejo, Krempyeng Yamuri, Pacar Keling, and Menur in Mulyorejo sub-district Surabaya were examined by Total Plate Count using pour plate method. The sample was also cultured in Mannitol Salt Agar. The colony suspected to be S. aureus were taken for identification. The identification of S. aureus consists of isolation in Mannitol Salt Agar, Gram staining, catalase test, and coagulase test. Total plate count result showed that four samples were exceeding the National Standard of Indonesia SNI 7388:2009 or 1x106 CFU/g and the rest were below the maximum Total Plate Count in SNI. The highest Total Plate Count result was 1,9x106 CFU/g and the lowest was 7,8x104 CFU/g. The result of identification showed that 100% samples examined were contaminated by S. aureus with the highest result was 2,9x104 CFU/g and the lowest result was 4,3x103 CFU/g or exceeding the SNI 7388:2009.
Toxicity Testing Of White Pomegranate (Punica granatum L.) Fruit Extracts Using Brine Shrimp Lethality Test Method As A Candidate Of Anti-Cancer Drug Ferian Firnanda; Eduardus Bimo Aksono Herupradoto; Kadek Rahmawati; Rochmah Kurnijasanti; Mohammad Sukmanadi; Nove Hidajati
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31810

Abstract

This research aimed to determine the effects of toxic white pomegranate fruit extract (Punica granatum L) against larvae of brine shrimp (Artemia salina Leach) indicated LC50 values below 1000 µg/ml. This study is purely experimental by using Brine Shrimp Lethality Test (BSLT). The study was divided into seven groups, namely ethanol extract of white pomegranate fruit (Punica granatum L) with a concentration of 31,25; 62,5; 125; 250; 500; 1000 µg/ml and negative control (seawater). Mortality data percentage of Artemia salina Leach analyzed by probit analysis. Results showed that the extract of white pomegranate fruit extract (Punica Granatum L) has a toxic effect with LC50 values of 248,6 µg/ml calculate from probit analysis. From these results, it can conclude that white pomegranate extract is toxic to larval shrimp (Artemia salina Leach) with Brine Shrimp Lethality Test (BSLT) method which means white pomegranate extract has the potential to be an anticancer drug.
Protection Effectiveness Of Bit (Beta vulgaris) Fruit Extract In Maintaining Spermatogenesis Ability In White Rats (Rattus novergicus) Induced Carbon Tetracloride (CCl4) Alvyan Lantang Anugrah; Hana Eliyani; Budi Utomo; Suherni Susilowati; Maslichah Mafruchati; Rochmah Kurnijasanti
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31812

Abstract

The aim of this research was to know whether beetroot (Beta Vulgaris) extract could protect spermatogenesis by maintaining spermatogenic and sertoli cell count  in rats (Rattus novergicus) induced with CCl4. Rats were given beetroot extract daily, for 14 days and 3ml/kg BW CCl4 intraperitoneally one hour after last treatment. This study used twenty rats which were devided equally into 5 groups. K(-), the negative control group was not induced with CCL4 and only given 1% CMC-Na suspension. K(+), the positive control group was induced with CCl­4 and given 1% CMC-Na suspension. P1, P2 and P3 were given beet root extract with doses of 200, 400, and 800 mg/kg BW daily before feeding. All of the beetroot treatment were given orally (2 ml). After 24 hours CCl4 induction, rats were sacrificed and testis were collected to make histology slides. The observations showed significantly different (p<0,05) in all of variables. Result showed significant differences in spermatogenic and sertoli cells between K(-) and K(+) groups, K(+) with P2 and P3 group, and showed insignificant difference between P2 and P3 group in spermatogenic and sertoli cells. The result of this research showed that beetroot extract could protect the spermatogenic and sertoli cells in male rats induced with CCl4.
The Anthelmintic Activity Of Etanol Extract of Porang Tubers Against Fasciola gigantica In Vitro Firanda Agustina; Rochmah Kurnijasanti; Lucia Tri Suwanti; Nunuk Dyah Retno Lastuti; Kadek Rachmawati; Lilik Maslachah
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31235

Abstract

This study aims to determine the anthelmintic activity ethanol extract of porang tubers (A. oncophillus) against F. gigantica worms in vitro. There were five treatments and each treatment was done in five replications and used 10 F. gigantica. The treatments of this research were K- with CMC Na 1%, K+ with Albendazole 2.4 mg/ml, P1 with extract concentration 5%, P2 with extract concentration 10%, P3 with extract concentration 20%. The results showed that the extract of Porang tuber (A. oncophillus) had an anthelmintic effect against F. gigantica worms in vitro. In the extract with a concentration of 20%, there were anthelmintic properties that almost the same as Albendazole. The higher the extract concentration, the higher the anthelmintic properties. The longer the immersion time, the higher the number of dead worms. The morphological changes was evaluated by light microscopic examination and the results showed many histopathological changes on the morphology of F. gigantica. The results indicate that the possible use of the tubers as a potential anthelmintic against F. gigantica.
Acute Toxicity Test Of The Green Viper Snake (Trimeresurus albolabris), Macroscopic Description Of The Kidney And Liver Of Mice (Mus musculus) Moch. Rizky Darmawan; Dadik Rahardjo; Wiwiek Tyasningsih; Rochmah Kurnijasanti; Djoko Legowo; Boedi Setiawan
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31135

Abstract

According to WHO (2010), the Trimeresurus albolabris snake is a venomous snake with a high number of bite cases in Indonesia. Information on the character and level of toxicity of snake venom in Indonesia, including Trimeresurus spp. snakes, is still minimal. This study aims to determine the LD50 value (lethal dose 50), clinical symptoms, and to determine the macroscopic appearance of the liver and kidneys of mice (Mus musculus) after being injected by a the white-lipped pit viper (Trimeresurus albolabris) venom. The LD50 value of Trimeresurus albolabris venom injected subcutaneously into mice was 5,09 mg/kgBW. Clinical symptoms that appear increased movement activity, increased respiratory rate, lethargy, decreased activity, and reduced grooming. Macroscopic appearance of the liver that looks abnormal is found (1) color changes in the form of pale, pale yellowish, and red spots (petechiae). (2) changes in surface structure found only one liver that is not flat. (3) changes in consistency in the form of brittle easily. In the kidney that looks abnormal, found (1) changes in color in the form of pale. (2) changes in surface structure were only found in one kidney in the form of nodules. (3) changes in consistency in the form of brittle easily and soft.
Taurine Antioxidant Effect in Decreasing Kidney Damage in Male Mice (Mus Musculus) due to Oxidative Stress Induced by Paraquat Ricki Pratama; Eka Pramyrtha Hestianah; Thomas Valentinus Widiyatno; Dewa Ketut Meles; Rochmah Kurnijasanti
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31811

Abstract

Paraquat toxicity occurs through the high production of reactive oxygen species (ROS) which cause damage due to oxidative stress. Antioxidants can reduce damage through prevention of oxidative stress. Taurine has shown the ability to act as an antioxidant. The aim of this research is to find a effect of antioxidant taurine to decrease kidney damage caused by oxidative stress due to paraquat by looking at the histopathology changes. 25 male adult mices from strain DDY were used and divided into five treatment groups; C(-) (Aquadest IP), C(+) (Paraquat 30mg/kg), P1 (Paraquat 30mg/kg + Taurine 250mg/kg), P2 (Paraquat 30mg/kg + Taurine 500mg/kg) and P3 (Paraquat 30mg/kg + Taurine 1000mg/kg). All groups were given treatment intraperitoneal for twenty one days. The mices were sacrificed where kidney were collected for histopathology preparation. The parameters measured were renal histopathological changes in form of degeneration and necrosis. The results show that taurine administration had an effect on decreased degrees of damage to kidney tubular cells, with a decrease in the mean degree of renal tubular degeneration and necrosis. Degeneration of renal tubular cells in groups (P2) reduced compared to the group (C+) there were significant differences (P <0.05). Necrosis of renal tubular cells in groups (P1, P2) reduced compared to the group (C+) there were significant differences (P <0.05).  In conclusion, this research proves that administration of paraquat causes renal histopathological changes which are characterized by degeneration and necrosis. It also proves that taurine dose of 500 mg/kgBB could provided optimal effect.
Detection of eae Gene as A Marker Strain EPEC (Enteropathogenic Escherichia coli) in Chicken Meat Sold at Some Traditional Markets in Surabaya Amirul Muslim Amrullah; Fedik Abdul Rantam; Dadik Raharjo; Wiwiek Tyasningsih; Agnes Theresia Soelih Estoepangestie; Martia Rani Tacharina
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.31134

Abstract

This study aimed to detect the presence of eae gene in E. coli from chicken meat sold at the traditional market Surabaya. Identification of bacteria was carried out in several stages, inoculated on pre-enrichment media using Buffered Peptone Water (BPW), planting on Levine’s Eosin Methylene Blue Agar (L-EMB) media, suspected colonies on L-EMB were subjected to Triple Sugar Iron Agar (TSIA) and Indol test for the confirmation of E. coli. The confirmed E. coli isolates were tested by Polymerase chain reaction (PCR) to determine the presence of the eae gene. The results showed that E. coli could be identified in 28 out of 100 (28%) chicken meat samples, and eae gene was detected in three E. coli isolates (11%).

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