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EFEK PENGGUNAAN PROBIOTIK Lactobacillus casei DAN Bifidobacterium sp TERHADAP ANGKA KONVERSI PAKAN DAN KONSUMSI PAKAN PADA AYAM PETELUR YANG DIINFEKSI Escherichia coli Nabil Fariz Noorrahman; Widya Paramita Lokapirnasari; Mohammad Anam Al arif; Koesnoto Soepranianondo; Sri Hidanah; Sarmanu Sarmanu
BRILIANT: Jurnal Riset dan Konseptual Vol 4, No 2 (2019): Volume 4 Nomor 2, Mei 2019
Publisher : Universitas Nahdlatul Ulama Blitar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (256.997 KB) | DOI: 10.28926/briliant.v4i2.288

Abstract

Penelitian ini bertujuan mengetahui probiotik Lactobacillus casei dan Bifidobacterium sp terhadap rasio konversi pakan dan konsumsi pakan pada ayam petelur yang diinfeksi escherichia coli. Dalam penelitian ini menggunakan sebanyak 120 ekor ayam petelur umur 25 minggu dengan  menggunakan metode percobaan desain Rancangan Acak Lengkap (RAL) pola faktorial. Probiotik L.casei 108 CFU/ml dan Bifidobacterium sp 108 CFU/ml diberikan seacara oral melalui air minum sebanyak 250 ml air/ekor/hari. Hasil penelitian efek probiotik Lactobacillus casei dan Bifidobacterium (p <0,05) konsumsi pakan tidak menunjukkan perbedaan yang nyata (p>0,05). Konsumsi pakan tidak menunjukkan perbedaan antara perlakuan dan kontrol yaitu berkisar 113,72 sampai 114,22  g/ekor/hari. Konversi pakan menunjukkan perbedaan yang nyata p<0,05 terhadap FCR. Terdapat interaksi antara infeksi dengan feed additive p<0,05 terhadap FCR.
The Difference of Results in Abdomen and Anus Area Measurement With Non-Contact Infrared Thermometer Anna Surgean Veterini; Nancy Margarita Rehatta; Hamzah Hamzah; Widijiati Widijiati; Sarmanu Sarmanu; Subijanto Marto Soedarmo; Widodo Jatim Pudjirahardjo; Annis Catur Adi; Heni Rachmawati; I Ketut Sudiana
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (127.022 KB) | DOI: 10.19087/jveteriner.2021.22.2.183

Abstract

Study about sepsis is chalenging to get the right therapeutic method. One clinical signs of the sepsis mice model is changes in body temperature. An easy way to examine body temperature is using infrared non-contact thermometer. The aim of this study is to compare the body temperature obtained from infrared non-contact thermometer at the abdomen and anus area. We used male Mus musculus mice, body weight of 25–30 grams, were divided into 2 groups (control and treated groups). In the control group, mice were injected with NaCl 0.9% solution, with the amount of NaCl volume equal to LPS. In the treated group, mice were injected with 2.5 mg/kgBW of LPS intraperitoneally. Body temperature measurement was measured in abdomen (tabd) and anus (tan) area. Body temperature was measured at 8th and 24th hour after LPS or NaCl injection. Body temperature value result using tabd was higher than tan. LPS injection to mice produced an increase in body temperature but was not significant when compared to the control group (8th and 24th hour). The mean difference between tabd and tan in 8th hour control groups respectively were 2.12oC. The mean difference between tabd and tan in 24th hour control groups 4.6oC. The mean difference in treated groups (8th hour) was 4.66oC, while it was 4.77oC in the 24th hour treated groups. LPS could caused vasodilation of the vessels, the measurement area of non contact-infrared thermometer will be the most important factor to be considered; tan may closer to the physiology condition.
PENGARUH MEDROKSI PROGESTERON ASETAT TERHADAP ULTRASTRUKTUR SEL LEYDIG TIKUS PUTIH (Rattus norvegicus) Sarmanu
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 1 (1999): December 1999
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/527

Abstract

The objective of the research was to study the effect of medroxy progesterone acetate on the ultrastructure of Leydig cells of Rattus norvegicus. Randomized complete design was applied to study. Treatment consisted of 4 doses medroxy progesterone acetate were 0, 4, 8 and 12 mg per rat respectively and 8 replications. Injection were applied perform 4 times per rat within 4 weeks interval. The testicle were examined ultrastructure change on the nucleus, endoplasmic reticulum, mitochondria and golgy apparatus of the Leydig cells. Results indicated that injections of medroxy progesterone acetate of 8 and 12 mg per rat causes significant untrastructure change of nucleus, endoplasmic reticulum, Golgi apparatus and mitochondria of Leydig cells in comparison with doses 0 and 4 mg per rat. In additions, there were no significant different in ultrastructure of Leydig cells between doses 8 and 12 mg per rat as well as there were no significant different between doses of 4 and 0 mg per rat.
Prevalence of Nematodiasis in Omasum and Abomasum of Goats Slaughtered at Pegirian Surabaya Slaughter House Through Gatrointestinal Surgery Method Dewi Mariyam; Sri Mumpuni Sosiawati; Kuncoro Puguh Santoso; Setiawan Koesdarto; Sarmanu Sarmanu; Ira Sari Yudaniayanti
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (533.67 KB) | DOI: 10.20473/jops.v2i1.16384

Abstract

The purpose of this research is to determine the kind of nematodes and to determine the prevalence result. Thirtysix samples of omasum and abomasum were collected from RPH Pegirian from September-November 2017 then using gastrointestinal surgery to get fresh adult worm, afterwards using Semichen-Acetic Carmine stainning method to makes the object easy to microscopic identify. The result showed that species was found Haemonchus contortus, as an identify result show that adult worm has a character on a anterior part called cervical papilae, female  worm has a barber’s pole on body also posterior part has a vulva flap, posterior part of male worm has a bursa copulatrix, spicula, gubernaculum and dorsal rays looks like Y shape. Seventeen positive samples and the prevalence rate was 47,2%, 273 adult worms and female worms 215 (78,75%) and the male worms 58 (21,25%). Chi-Square statistical test (P <0.05) showed that 13 positive samples of kambing kacang (65%) were higher infected Haemonchosis infection than 4 potitive samples of PE (25%).
Prevalance of Gastrointestinal Protozoa of Cats in Animal Hospital and Animal Clinic in Surabaya Amara Lintang Pagati; Lucia Tri Suwanti; Chairul Anwar Nidom; Wiwik Misaco Yuniarti; Sarmanu Sarmanu; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 2 No. 2 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (389.385 KB) | DOI: 10.20473/jops.v2i2.16401

Abstract

Research of gastrointestinal protozoan in cats at Surabaya are still limited. Protozoa infection of the gastrointestinal tract can cause diarrhea and even zoonosis. This research aimed to identify and determine the prevalence of protozoan in cats in animal hostptal and animal clinic in Surabaya. Ninety  fecal samples were collected from 2 animal clinic and one animal hospital. Samples were  examined e wet mount (native, sedimentation, and floatation) and (Ziehl Nellsen) stain. Protozoa was identified by using a light microscope with 400x and 1000x magnification. The result showed 68,89% of samples were positively infected by gastrointestinal protozoa. The protozoa were Blastocystis sp, Cryptosporidium sp, Giardia sp, and Eimeria sp. By chi square test, there was  not significant differences the prevalence of gastrointestinal protozoan in cat between sex, age, breed, and diarrhea status
Produksi dan Kualitas Telur Itik Alabio di Daerah Sentra Peternakan Desa Sungai Pandan, Kabupaten Hulu Sungai Utara, Kalimantan Selatan Rini Fajarwati; Sarmanu Sarmanu; Chairul Anwar Nidom; Sri Pantja Madyawati; Imam Mustofa; Mirni Lamid; Sri Hidanah; Widya Paramita Lokapirnasari; Tarzan Purnomo; Mohammad Sukmanadi
Jurnal Medik Veteriner Vol. 3 No. 2 (2020): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol3.iss2.2020.246-250

Abstract

Penelitian ini merupakan penelitian deskriptif dengan menggunakan 20 itik Alabio betina siap bertelur dengan rentang usia 6 bulan dan untuk pemeriksaan kualitas telur untuk masing-masing kelompok 10 sampel. Pengamatan tingkat produksi telur dilakukan selama tiga bulan dengan 10 pengulangan, kualitas telur (ketebalan kulit telur, persentase kulit telur, persentase kulit telur, persentase albumin, persentase kuning telur, huagh unit, warna kuning telur, kadar protein dan lemak kandungan). Data produksi telur dan kualitas telur diuji menggunakan uji Anova. Hasil pengamatan rata-rata produksi harian telur 15.45 ± 0.12, 14.72 ± 0.10, 14.91 ± 0.09; produksi berat harian (kg/hari) 925.27 ± 7.50, 875.40 ± 6.32, 884 ± 5.03; hasil dari ketebalan kulit telur kualitas 0.33 ± 0.01, 0.33 ± 0.12, 0.33 ± 0.12; persentase kulit telur 11.32 ± 0.93, 11.28 ± 0.10, 11.36 ± 0.05; persentase albumin 55.70 ± 1.08, 56.66 ± 1.22, 56.12 ± 1.00; persen kuning telur 32.07 ± 1.24, 31.55 ± 1.48, 31.64 ± 1.20; Haugh unit 88.70 ± 6.21, 91.41 ± 6.70, 94.51 ± 5.06. Warna kuning masing-masing memiliki skor 15. Dapat disimpulkan bahwa produksi dan kualitas telur itik Alabio baik.
Respon Klien Terhadap Pelayanan Elektroakupunktur Hewan di Klinik La Femur Surabaya Tutik Rosita Idayati; Koesnoto Supranianondo; Sarmanu Sarmanu; Raden Tatang Santanu Adikara; Wiwik Misaco Yuniarti; Suharsono Suharsono
Jurnal Medik Veteriner Vol. 3 No. 2 (2020): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol3.iss2.2020.173-181

Abstract

Elektroakupunktur merupakan metode modern dalam bidang akupunktur veteriner. Penerapan pada hewan dinilai efektif, ekonomis, tidak menimbulkan efek samping sehingga klien merasa puas. Kepuasan klien berdasarkan persepsi terhadap kualitas pelayanan yaitu kesesuaian antara harapan dan kenyataan yang diterima klien. Tangibel, reliability, responsiveness, assurance, dan empathy merupakan lima unsur dimensi kualitas pelayanan. Jumlah responden yang diperoleh dalam penelitian ini yaitu sebanyak 30 responden menggunakan layanan elektroakupunktur hewan sebanyak dua kali di klinik La Femur. Data yang diperoleh kemudian dianalisis menggunakan indeks kepuasan klien dan importance performance analysis. Hasil analisis data lima dimensi kualitas pelayanan elektroakupunktur hewan klinik La Femur menunjukkan nilai tangibel 0.91%, reliability 0.97%, responsiveness 0.99%, assurance 0.99% dan empathy 0.98%. Lima dimensi pelayanan elektroakupunktur hewan klinik La Femur menunjukkan nilai diatas standart.
Analysis of Marketing Opportunity Increase based on Consumer Criteria for Hydroponic Vegetable Producers in Surabaya Titik Yuliarini; Soeharsono Soeharsono; Mirni Lamid; Mohammad Anam Al Arif; Sarmanu Sarmanu; Sri Hidanah
Caraka Tani: Journal of Sustainable Agriculture Vol 35, No 2 (2020): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v35i2.29622

Abstract

Consumer is one of the influential factors in marketing. The study was conducted to analyze the increase in marketing opportunities based on consumer criteria for hydroponic vegetable producers in Surabaya. The research was carried out in Surabaya from Mei to June 2018. This type of research is observational analysis with survey methods on three producers about the relationship between producers and consumers according to their respective producers. Data were analyzed with AHP (Analysis Hierarchy Process) and the process used Expert Choice 2000. Data were examined using descriptive or qualitative analysis and quantitative analysis. Quantitative analysis includes a scale of trust and commitment measurement. The distribution of hydroponics vegetables at “Kebunsayur Surabaya” prioritizes ‘restaurant’ and ‘hotel’, “JawaRa Farm” prioritizes ‘kale suppliers’ and “One STOP Hydroponics” prioritizes ‘individual customers’ (vegetable displays).
Pengaruh Probiotik Bakteri Asam Laktat dalam Air Minum Terhadap Konversi Pakan Ayam Broiler Nanda Kholifatul Azizah; Sarmanu Sarmanu; Budi Utomo; Emy Koestanti Sabdoningrum; Widya Paramitha Lokapirnasari; Koesnoto Supranianondo
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28587

Abstract

This study aimed to determine the effect of probiotics of lactic acid bacteria in drinking water on feed consumption, weight gain and feed conversion ratio of broiler chickens. This research is an experimental research. The experimental animals used in this study were 28 male broiler chickens strain Cobb 700 aged 20 days. P0 was not given the addition of probiotic lactic acid bacteria, P1 was given the addition of probiotic lactic acid bacteria Lactobacillus acidophilus in 1ml/liter drinking water, P2 was given the addition of probiotic lactic acid bacteria Lactobacillus casei in drinking water 1ml/liter, P3 was given the addition of probiotic lactic acid bacteria Lactobacillus fermentum in drinking water 1ml/liter, P4 was given the addition of probiotic lactic acid bacteria Lactobacillus plantarum in drinking water 1ml/liter, P5 was given the addition of probiotic lactic acid bacteria Pediococcus in drinking water 1ml/liter, P6 was given the addition of probiotic mix lactic acid bacteria in drinking water 1ml /liter. Collecting data for the last 1 week by weighing the remaining consumption of feed given and weight gain of broiler chickens. The data obtained were analyzed using the Analysis of Variant (ANOVA) statistical method and continued with Duncan. Based on the results of the calculation of the amount of feed consumption and weight gain in broiler chickens, it showed that the administration of probiotic lactic acid bacteria 1ml/liter of water in broiler chickens could increase body weight gain, reduce feed conversion rates but did not reduce broiler feed consumption (p<0.05).
Effect of Aflatoxin Contaminated Feed for 40 Days on Anatomy and Histopathology of the Thymus of Laying Hens in the Starter Phase Rachella Christy; Soeharsono Soeharsono; Ismudiono Ismudiono; Hana Eliyani; Sarmanu Sarmanu; Hani Plumeriastuti
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28679

Abstract

The purpose of this study was to determine the effect of aflatoxin contaminated feed given for 40 days on the anatomy and histopathology of the thymus of laying hens in the starter phase. A total of 36 DOC laying hens were divided into two groups (P0 and P1). P0 (control group) was given 100% basal starter feed CP 521 and P1 (treatment group) was given 80% basal feed CP 524 plus 20% aflatoxin contaminated feed. Feeding contaminated with aflatoxin was carried out for 40 days, starting on the 20th to the 60th day after the DOC of laying hens was adapted for 20 days. The time of surgery in this study was carried out on the 20th, 40th, and 60th days, where at each surgery time the thymus organ was taken to collect weight data and histopathological preparations were made. The thymus weights based on time showed a significant decrease, but the intergroup thymus weights did not show a significant decrease. The decrease in thymus weight is caused by the involution process. Microscopic observations based on increased cell debris and congestion showed that P0 did not show a significant difference, but P1 showed a significant difference (p>0.05) on the 40th and 60th days. The conclusion in this study was that as much as 20% of feed contaminated with aflatoxins had not affected changes in thymus weight but had shown damage to the histopathological structure of the thymus.