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All Journal Microbiology Indonesia Jurnal Ilmu dan Teknologi Peternakan Tropis Majalah Ilmiah MOMENTUM Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy) Unnes Science Education Journal JURNAL EDUCATION AND DEVELOPMENT Inovasi: Jurnal Ilmiah Ilmu Manajemen Jurnal Riset Entrepreneurship Health Care : Jurnal Kesehatan Jurnal Penegakan Hukum Indonesia (JPHI) Jurnal Ilmiah Wahana Pendidikan Fidelity : Jurnal Teknik Elektro AGRONISMA Juru Rawat. Jurnal Update Keperawatan Homeostasis: Jurnal Mahasiswa Pendidikan Dokter JURNAL MUARA PENDIDIKAN Jurnal Sains dan Teknologi Farmasi Indonesia Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan EduChem Jurnal Informatika Polinema (JIP) Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
MARIA ULFAH
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Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Journal : Microbiology Indonesia

 1 
Cloning, Sequencing, and Expression of the Gene Encoding a Family 9 Cellulase from Bacillus licheniformis F11 in Escherichia coli and Bacillus megaterium, and Characterization of the Recombinant Enzymes IS HELIANTI; MARIA ULFAH; NIKNIK NURHAYATI; LLINA MULYAWATI
Microbiology Indonesia Vol. 8 No. 4 (2014): December 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (913.463 KB) | DOI: 10.5454/mi.8.4.2

Abstract

 A gene encoding cellulase belonging to the glycosyl hydrolase family 9 along with its native promoter was isolated from Bacillus licheniformis F11, cloned in Escherichia coli DH5 α and subcloned by transconjugation to Bacillus megaterium MS941. Functionality of the encoded protein was proven both in heterologous hosts, E. coli and B. megaterium. In the latter, the gene product was found in the extracellular fraction expressing a high specific activity; whereas in E. coli the protein was not secreted into the medium, and rather, showed a lower specific activity. The optimum temperature of the recombinant enzyme expressed in the hosts range from 65-75 ºC; whereas the optimum pH is 6. The recombinant enzyme was stable between 50-60 ºC and in a broad pH range (pH 5 - 9). Addition of Ca2+ and Fe3+ enhanced the enzyme activity, whereas EDTA and Cu2+  had the opposite effect. Lichenin, rather than carboxyl methyl cellulose, is the preferred substrate.
Polyoxygenated Diterpene Produced by The Indonesian Marine Sponge Callyspongiasp. as an Inhibitor of the Human Pancreatic Cancer Cells VIQQI KURNIANDA; SUCI FARADILLA; SOFYATUDDIN KARINA; SRI AGUSTINA; MARIA ULFAH; CHITRA OCTAVINA; FARAH SYAHLIZA; MUHAMMAD RIZKI RAMADHAN; SYAHRUL PURNAWAN; MUSRI MUSMAN
Microbiology Indonesia Vol. 13 No. 2 (2019): June 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (986.522 KB) | DOI: 10.5454/mi.13.2.5

Abstract

The bioactive compound from the Indonesian Marine SpongeCallyspongia sp.isolated based on bioassay-guided separation with several steps of chromatography has been done. There are 4 major compound known as isocopalane diterpene derivatives together with a plausible new compound, isocopalanol, a polyoxygenated isocopalane diterpene. Isocopalanol has molecular weight [M+H]+ 412.576 m/z indicate that the compound has molecular formula C24H44O5 determined by LCMS-ESI. This compound has bioactivity IC50 = 0.1 μg / mL against human pancreatic cancer cell. 
The utilization of auto-inducible Plyb promoter and media optimation for cell density-dependent expression of recombinant xylanase in Bacillus subtilis DB104 Haniyya Haniyya; Dini Achnafani; Maria Ulfah; Niknik Nurhayati; Is Helianti
Microbiology Indonesia Vol. 14 No. 1 (2020): March 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1582.039 KB) | DOI: 10.5454/mi.14.1.2

Abstract

Strong promoters are one of the fundamental aspects to increase the level of gene expression, and one of approach to improve the recombinant enzyme productivity so that the efficiency of production cost for enzyme production in industrial scale can be reached. Here we assessed the application of a cell density-dependent promoter and media optimation to promote cell growth and protein expression of Bacillus subtilis without excess usage of inducers. An auto-inducible Pylb promoter that is potential to provide inducer-free enzyme production was cloned and introduced into xylanase recombinant system in B. subtilis DB104 by PCR cloning and protoplast transformation. A 200 bp target gene was successfully inserted in between xynCM1 ORF -coding for B. halodurans CM1 xylanase- and its native promoter sequence at the upstream region. The disruption of the native promoter was intended to replace the native promoter with Pylb. Recombinant xylanase gene under Pylb was successfully expressed in B. subtilis DB104 and the enzyme was produced at stationary phase. Different media with various concentrations of glucose and nitrogen were used to optimize recombinant xylanase expression. It achieved a higher level of xylanase expression compared to wild-type and recombinant xylanase with native promoter B. subtilis in media containing a 2-fold recipe of LB media thus leads to increase cell density and xylanase expression (81.461 U mL-1).
Co-Authors Aang Hanafiah Wangsaatmadja Abdul Basit achmadi achmadi Ady Laksono Putro Agung Ary Wibowo Ali Harokan Amalia Nurul ‘Ulum Andi Sri Irtwaty Annis Mu’awwanah Aristha Puji Wahyuningrum Asnath Maria Fuah, Asnath Maria Atip Nurwahyunani Budi Widiyanto Chitra Octavina Devi . Dhia Salsabilla Dinar Putri Octaviani Dini Achnafani Dwi Kartini Edi Hartoyo EDIATI SASMITO Edwin Mirzachaerulsyah Efa Erfiana Safitri Evi Sukawati Fajar Karenia Sawindu FARAH SYAHLIZA Farida Heriyani Firmansyah, Haris Fitri Andhriani fitriandhriani Friska Abadi Gilang Prastyca Hairida Haniyya Haniyya herni widyowati Husni Syahrudin Ida Yuliana Ika Kustiyah Oktaviyanti Imran Imran Indira Kinasih Indranila Indranila Ira Lestari IS HELIANTI Ismiyyatun Khasanah Iwan Ramadhan Khairini Putri Kiki Rizqi Amalia Lena Rosida Lilis Suryani LLINA MULYAWATI Luhur Pujo Santoso M. Khairun Iffat Masriani . Maulina Ayu Meidiastuti Metalia Erny Muhammad Agus Hardiansyah Muhammad Itqan Mazdadi Muhammad Rizki Ramadhan Musri Musman Nahdhah Nahdhah Neisya Benhadj NIKNIK NURHAYATI Nirmalasari Nirmalasari Norisnaniah Norisnaniah Nur Riska Safitri nurul fakhriyah Oktarina Heni Puspitowati Penni Dwi Royani Perdana, Adli Waliul Rachmat Sahputra Rahma Laila Qodriyan Sofiakmi Rahmat Rasmawan Riama Al Hidayah Ricky Chandra Kurniawan Risya Sasri Rita Febrianti_Lidya Putra Saragih, Triando Hamonangan Sofyatuddin Karina Sri Agustina SUCI FARADILLA Sumantri ) Sumantri Sumantri Suriyanisa Suriyanisa Syahrul Purnawan Viqqi Kurnianda Vitri Sari Nur Cahyani Widya Sari Winarno Winarno Wiwiet Susan Amelia Yulianti Rospina Yuni Cahya Endrawati