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All Journal International Journal of Evaluation and Research in Education (IJERE) Jurnal Teknologi Industri Pertanian MANAJEMEN IKM: Jurnal Manajemen Pengembangan Industri Kecil Menengah Jurnal Rekayasa Proses Microbiology Indonesia Jurnal Pendidikan Humaniora Jurnal Penelitian Pascapanen Pertanian Buletin Teknologi Pasca Panen Jurnal Riset Industri Jurnal Aplikasi Bisnis dan Manajemen (JABM) E-Journal Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan Syntax Literate : Jurnal Ilmiah Indonesia Jurnal Penjaminan Mutu Visipena Jurnal Organisasi Dan Manajemen Jurnal Pengabdian Masyarakat AbdiMas Jurnal Rekayasa Proses International Journal of Supply Chain Management JURNAL TEKNIK INDUSTRI Bulletin of Social Informatics Theory and Application
Illah Sailah
Departemen Teknologi Industri Pertanian, Fakultas Teknologi Pertanian, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Social Sciences Transportation

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Journal : Microbiology Indonesia

 1 
The Process of Xylanase Production from Bacillus pumilus RXAIII-5 NUR RICHANA; TUN TEDJA IRAWADI; M. ANWAR NUR; ILLAH SAILAH; KHASWAR SYAMSU
Microbiology Indonesia Vol. 1 No. 2 (2007): August 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.944 KB) | DOI: 10.5454/mi.1.2.6

Abstract

The optimum conditions for the growth of Bacillus pumilus RXAIII-5 (a potential xylanase producer) were sought, these included temperature, pH, aeration, and agitation of the culture batch. Afterwards a mathematical model based on the parameter of cultivation kinetics was formulated. At the same time, the rheology of the fluid used for bacterial cultivation in a bioreactor was studied. The data obtained was used for estimating the ‘scaling up’ of enzyme production. The results of the study indicate that the optimum condition for processing in 50 ml Erlenmeyer flask are used temperature of 35 oC (308oK), pH 7, and an agitation rate of 140 rpm. The highest xylanase activity and its specific activity are 297.132 U.ml-1 and 655.32 U.g-1protein, respectively. Subsequent experiments in a bioreactor using all of the experiment parameters mentioned above, except for the agitation rate, shows that the results are as follows. The highest specific growth was at 0.082 hour-1 at an aeration and agitation rate of 0.5 vvm and 150 rpm, respectively. Based on the data of the cultivation kinetics, the optimum conditions for the fermentation in Biostat 2L-bioreactor is 1 vvm and 200 rpm of aeration and agitation, respectively . The efficiency of substrate (Yp/s) and of cell biomass (Yp/x) to produce xylanase is 50.744 U.g-1 and 43.906 U.g-1, respectively. The efficiency of substrate to cell production (Yx/s) is 1.178g.g-1. The liquid cultivation-medium has non-Newtonian properties. Based on a mathematical model it is found that the consistency index (k constant) and index of liquid behavior (n value) are 0.179 g.cm-1.second-1 and 0.3212, respectively. Becouse the value of 0
Co-Authors - Aslichan - Farida Ahmad Fuad Akrom, Akrom Alex Denni Amril Aman Andes Ismayana dan Moh. Rizal Afriyanto Ani Suryani Anis Annisa Adnan Anwar Nur Arfian Muslim Ari Pambudi Arie Wibowo Khurniawan Asrul Siregar Bambang Indriyanto Bambang Indriyanto Bambang Indriyanto Bambang Sukaca Bayu Koen Anggoro Budy Wiryawan Chairunnisa Dedy Sugiarto Dian Pratomo E Gumbira Sa, E Gumbira Elisa Anggraeni Elisa Anggraeni Erry Yudhya Mulyani Fitri Mulyaningsih Fredinan Yulianda Habibullah Habibullah Hanoch Maurizio Hetharia Harsasi, Mairani Hasan Supriadi Hendri Utami Husni Y Rosadi, Husni Y Ichwanudin, Muhammad Ika Amalia Kartika Ikbal Rachmat Imam Yuwono Irman Hermadi Khaswar Syamsu Lukman M. Baga Luluk Sulistiyo Budi M Hasjim Bintoro M Syamsul Ma’arif M. ANWAR NUR M. Syamsul Maarif M. Syamsul Maarif M. Syamsul Maarif M. Syamsul Ma’arif Manuntun Parulian Hutagaol Marimin . Marimin Marimin Maulana Miladulhaq Meika S. Rusli Muhammad Romli dan Suprihatin Andes Ismayana Muhammad Syamsun Mursiti Mursiti Musa Hubeis Nanda Lega Jaya Putra Nastiti Siswi Indrasti Nasution, Efrida Yanti Nefianto, Tirton nFN Risfaheri Nugroho, Tezar Mega Nur Richana Okta Amelia Ono Suparno dan Eko Wahyudi Praja, Arief Kusuma Among Pudji Muljono Purwani Puji Utami, Purwani Rizal Syarief Safriyana Safriyana Sapta Raharja dan Maya Dwiyuni Saptadinata, Abi Sri Hartoyo Suharto Honggokusumo Sukardi Sukardi Syafa’at Ariful Huda, Tarigan, Mazmur Tatang Hidayat Titi C Sunarti Titi C. Sunarti Tun Tedja Irawadi Tyara Puspaningrum Ummanah Ummanah, Ummanah Vioreza, Niken Wasrin Syafii dan Kurnia Sofyan Gustan Pari Buchari Wijaya, Rahmat Yandra Arkenan Yazid Bindar Zainal Alim Mas'ud