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All Journal Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Jurnal Kedokteran Brawijaya JURNAL WIDYA MEDIKA Jurnal Ilmiah Kursor Indonesian Journal of Urology Berkala Ilmu Kesehatan Kulit dan Kelamin Folia Medica Indonesiana Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Cancer Majalah Obstetri dan Ginekologi Medical Laboratory Technology Journal Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Jurnal Manajemen Kesehatan Yayasan RS.Dr. Soetomo Majalah Patologi Indonesia Prosiding Seminar Nasional Multidisiplin Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Majalah Biomorfologi (Biomorphology Journal) Indian Journal of Forensic Medicine & Toxicology Indonesia Journal of Physical Medicine and Rehabilitation Indonesia Journal Physical Medicine and Rehabilitation Cermin Dunia Kedokteran Indonesian Journal of Cancer

Willy Sandhika

Departemen Patologi Anatomi, Fakultas Kedokteran Universitas Airlangga

Author-ID : 370004

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Other

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Gambaran Histopatologi Nekrobiosis Lipoidika Sari, Maylita; Setyaningrum, Trisniartami; Sandhika, Willy
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol 26, No 1 (2014): BIKKK APRIL 2014
Publisher : Faculty Of Medicine Airlangga University

Latar belakang: Nekrobiosis Lipoidika (NL) merupakan keadaan dermatologis idiopatik yang menjadi masalah secara kosmetik dan berhubungan erat dengan penyakit diabetes mellitus. Pemahaman tentang patogenesis dan diagnosis NL sangat penting karena adanya komplikasi yang bisa terjadi seperti ulserasi dan karsinoma sel skuamosa, disamping kesulitan penegakan diagnosis NL melalui histopatologi. Tujuan: Memahami patofisiologi, gambaran klinis, dan histopatologi NL serta diagnosis banding granuloma non infeksi. Telaah kepustakaan: Perubahan vaskular dan degenerasi kolagen serta faktor lain adanya Antibody Mediated Vasculitis diduga terjadi pada patogenesis NL. Prinsipnya didapatkan kerusakan mikrosirkulasi pada individu non diabetik dengan NL. Pemeriksaan histopatologi menunjukkan terdapat granuloma interstitial dan palisade yang melibatkan jaringan subkutan dan dermis. Granuloma tersusun berlapis-lapis (tierlike, layered) dan bercampur dengan area degenerasi kolagen, terdiri dari histiosit, beberapa diantaranya limfosit berinti banyak, sel plasma, dan eosinofil. Penebalan dinding pembuluh darah dan pembengkakan sel endotel pada dermis bagian tengah sampai dalam. Imunofluoresens direk didapatkan immunoglobulin M, Ig A, C3, dan fibrinogen pada pembuluh darah. Manifestasi klinis ditandai dengan plak berbatas jelas dengan area atrofi kekuningan pada tengahnya dan tepi menonjol berwarna merah sampai ungu, yang bisa mengalami ulserasi, bilateral, dan multipel. kesimpulan: NL merupakan suatu penyakit degeneratif kronis pada jaringan konektif dermis, dengan etiologi yang tidak diketahui dan terjadi hampir seluruhnya pada penderita diabetes mellitus. Diagnosis ditegakkan secara klinis dan histopatologi. Gambaran histologi NL hampir sama dengan granuloma annulare, namun manifestasi klinis berbeda.Kata kunci: nekrobiosis lipoidika, diabetes melitus, histopatologi, granuloma.

Perbedaan Dermatitis Seboroik dan Psoriasis Vulgaris Berdasarkan Manifestasi Klinis dan Histopatologi Astindari, Astindari; Sawitri, Sawitri; Sandhika, Willy
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol 26, No 1 (2014): BIKKK APRIL 2014
Publisher : Faculty Of Medicine Airlangga University

Latar belakang: Dermatitis seboroik (DS) dan psoriasis sering sulit dibedakan baik secara klinis maupun secara histopatologi. Anamnesis yang tepat dengan memperhatikan usia, riwayat keluarga, dan pemeriksaan klinis yang teliti serta ditunjang dengan pemeriksaan histopatologi, dapat menentukan diagnosis yang tepat. Tujuan: Mengevaluasi perbedaan DS dan psoriasis supaya klinisi dan patolog bisa membuat diagnosis yang benar.Telaah kepustakaan: Secara epidemiologi, terdapat berbagai perbedaan antara DS dan psoriasis. Hal itu bisa dilihat dari usia saat timbulnya lesi, jenis kelamin, ras, maupun genetik. Lokasi lesi dan manifestasi klinis juga mempunyai ciri yang berbeda. Biopsi kulit dibutuhkan untuk membantu menegakkan diagnosis yang tepat.Gambaran histopatologi DS bervariasi sesuai dengan perjalanan penyakitnya: akut, sub-akut, dan kronis, sedangkan psoriasis mempunyai ciri khas berupa pemanjangan rete ridges, abses Munro atau adanya abses Kojog. Simpulan: Terdapat beberapa perbedaan antara DS dan psoriasis dari usia pertama kali muncul lesi, lokasi lesi, manifestasi klinis dan gambaran histopatologi.Kata kunci: dermatitis seboroik, psoriasis, manifestasi klinis, gambaran histopatologi.

Comparison of TNF-α and TGF-β Expression in Breast Cancer Tissues-with and without Axillary Lymph Node Metastasis Tyasmara, Ayu; Sandhika, Willy
Indonesian Journal of Cancer Vol 12, No 1 (2018): Jan - Mar
Publisher : National Cancer Center - Dharmais Cancer Hospital

Background: Breast carcinoma dynamics can be seen from the inflammatory mediator produced and some of expressed cytokine mediators mostly found in breast cancer tissues are TNF-α and TGF-β. This research analyzed TNF-α and TGF-β influence in axillary lymph nodes metastasis to prove the role of cytokines in breast cancer dynamics and to be taken as prognosis. Methods: This research used paraffin block of breast carcinoma in various stages of axillary lymph nodes metastasis and TNF-α and TGF-β antibody to observe the expressions. Result: There were significant differences in TNF-α expressions improvement with p < 0.05 in lymph nodes metastasis N1 (13.18%) and TNF-α expressions reduction in lymph nodes metastasis N2 (52.27%) and N3 (27.27%) compared with lymph nodes without metastasis (52.73%). There were no significant differences in TGF-β expressions of all N groups. Conclusion: The result stated that TNF-α cytokines can be used as biomarker independently predicting breast carcinoma prognosis and progressivity. ABSTRAKLatar Belakang: Dinamika karsinoma payudara tercermin dari mediator inflamasi yang dihasilkannya dan salah satu mediator sitokin yang terekspresi banyak pada jaringan tumor payudara adalah TNF-α and TGF-β. Kami menganalisis pengaruh TNF-α dan TGF-β pada metastasis kelenjar getah bening aksila untuk membuktikan peran sitokin dalam dinamika kanker payudara dan untuk dimanfaatkan sebagai alat prognosis. Metode: Penelitian ini menggunakan blok parafin karsinoma payudara pada berbagai stadium metastasis kelenjar getah bening aksila dan menggunakan antibodi TNF-α dan TGF-β untuk mendeteksi ekspresinya. Hasil: Hasil penelitian ini didapatkan perbedaan peningkatan ekspresi TNF-α yang signifikan dengan p < 0,05 pada metastasis kelenjar getah bening N1 (73,18%) dan penurunan ekspresi TNF-α pada metastasis kelenjar N2 (52,27%) dan N3 (27,27%) dibandingkan dengan tanpa metastasis (52,73%). Tidak ada perbedaan signifika n pada ekspresi TGF-β antar semua kelompok N. Kesimpulan: Hasil ini menyatakan sitokin TNF-α dapat dipakai sebagai biomarker untuk memprediksi progresifitas dan prognosis karsinoma payudara secara independen

Tulsi (Ocimum sanctum) Leaf Ethanol Extract Reduces Inflammatory Cell Infiltration in Aspirin-Induced Gastritis Rats Sari, Festi Artika; Sandhika, Willy; Yuliawati, Tri Hartini
Jurnal Kedokteran Brawijaya Vol 31, No 1 (2020)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Gastritis is an inflammation of the gastric mucosa. Tulsi leaf extract has phenol, flavonoid and saponin compounds which are potential as antioxidant and increase defensive factors in the gastric. The purpose of this research was to find out the effect of tulsi (Ocimum sanctum) leaf extract in polymorphonuclear (PMN) inflammatory cell infiltration in gastric of aspirin-induced gastritis rat model. This study was laboratory experimental research using post-test only control group design. Randomly, 27 male rats were divided into 3 groups, the first group was not induced by aspirin and extract as negative control, the second group was induced by aspirin of 600 mg/kgBW as positive control, and the third group was induced by aspirin of 600 mg/kgBW and was given Ocimum sanctum extract at a dose of 400 mg/kgBW as treatment group. Gastric of the rats were taken on 16th day for histopathology evaluation using hematoxylin and eosin (HE) staining. Evaluation was done by calculating the PMN inflammatory cell infiltration in mucosal and submucosal layer. The results of the average number of PMN inflammatory cell in the gastric tissue of the treatment group showed a significant decrease compared to the positive and negative control groups with P-value <0.05. This study proved that Ocimum sanctum leaf extract administration with the dose of 400 mg/kgBW can decrease gastritis inflammation by reducing PMN inflammatory cell in gastric of aspirin-induced gastritis rat model.

Analysis of Cell Block and Cytology Specimen Preservation from Lung Aspiration Biopsy Poernomo, Adinda Sandya; Sandhika, Willy; Budipramana, Vicky Sumarki
Jurnal Kedokteran Brawijaya Vol 31, No 1 (2020)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Cytology smear technique is often used in Indonesia because the process is safe, simple, easy, fast, and cost effective. At present, several studies have found that smears with cell block techniques are of better quality than smears with cytology smear techniques. The aim of this study was to analyze whether the cytology smear technique can produce adequate specimens compared to cell block towards results of lung Fine Needle Aspiration Biopsy (FNAB). Lung FNAB specimens were divided into two parts: one part was processed with cytology and the other part with cell block technique. The specimens were observed under a microscope to count the number of inflammatory cells and the number of artifacts. The numbers of inflammatory cells and artifacts were scored 0-3. The inflammatory cells consisted of neutrophils, lymphocytes and plasma cells, also macrophages. The result showed no significant difference between the number of inflammatory cells in cytology and cell block (p neutrophils=0.543; p lymphocytes and plasma cells=0.192; p macrophages=0.487) in 38 samples. The artifact score comparison test result showed a significant difference between the number of artifacts in cytology and cell block (p=0.027) with more artifacts in cytology. The most common artifact in cytology was air bubble artifacts, while cell block was dominated by torn pieces artifacts. There was no significant difference between the number of inflammatory cells found in cytology and cell block techniques. Cell block technique has less artifacts than cytology, but artifacts found in cytology can be corrected so that the cytology smear technique is still an option.

Effect of Javanese Turmeric (Curcuma xanthorrhiza) Extract on Hepatitis Model of Alcohol-Induced Mice Puteri, Agnes Ilene Suprapto; Sandhika, Willy; Hasanatuludhhiyah, Nurina
Jurnal Kedokteran Brawijaya Vol 31, No 1 (2020)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Alcoholic hepatitis is a common cause of non-viral hepatitis. Javanese turmeric (Curcuma xanthorrhiza) contains xanthorrhizol and curcuminoid that have antioxidant and anti-inflammatory effects. The purpose of this research was to detect the effect of Curcuma xanthorrhiza in decreasing hydropic degeneration and lymphocytes in alcohol-induced mice. This experimental research used post-test only controlled design. Randomly, 35 mice were divided into five groups. Four groups were induced by alcohol 30% of 4ml/kgBW in 10 days, and one group was normal group (negative control). Three of the four alcohol-induced groups were given Curcuma xanthorrhiza extract at doses of 42, 84, and 168 mg/kgBW in 10 days, 1 hour after alcohol induction. Mice livers were taken for Hematoxylin Eosin (HE) staining.Â Hydropic degeneration and lymphocytes in the liver lobules were examined under a microscope. The statistical analysis used in this research was the Kruskal-Wallis, the Mann-Whitney test, and the Independent Sample T Test. P-value is significant if <0.05. The result of this research were all doses of Javanese turmeric extract significantly reduced hydropic degeneration. Doses of 84 and 168 mg/kgBW also reduced inflammatory lymphocytes (p<0.05). The dose of 168 mg/kgBW reduced the lymphocyte inflammation maximally. There was no significant difference between increasing Javanese turmeric dose and decreasing hydropic degeneration and lymphocytes (p>0.05). This research shows that Javanese turmeric (Curcuma xanthorrhiza) can attenuate hepatitis by decreasing hydropic degeneration and lymphocytes in alcohol-induced liver.

Hubungan antara Kadar Prostate Specific Antigen Serum dan Skor Gleason pada Adenokarsinoma Prostat Navisa, Claudia Clary; Sandhika, Willy; Arwati, Heny
Jurnal Kedokteran Brawijaya Vol 30, No 3 (2019)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2019.030.03.3

Kanker prostat adalah kanker terbanyak ke empat di dunia dan merupakan kanker terbanyak ke dua yang menyerang pria. Pada tahun 2013, angka kejadian kanker prostat di Indonesia adalah 25.012 dengan jumlah kasus terbesar yaitu di Jawa Timur sebanyak 5.668. Prostate Specific Antigen (PSA) sebagai penanda tumor, dikatakan berhubungan secara linier dengan skor Gleason. Tujuan dari penelitian ini adalah untuk mengetahui hubungan antara kadar PSA serum dengan skor Gleason pada pasien kanker prostat di RSUD Dr. Soetomo. Penelitian ini merupakan studi analitik dengan desain belah lintang. Data penelitian dikumpulkan dari data rekam medis pasien kanker prostat yang menjalani pemeriksaan kadar PSA serum dan histopatologi jaringan prostat di RSUD Dr. Soetomo Surabaya pada tahun 2015-2017. Pasien dengan Benign Prostate Hyperplasia, infeksi saluran kencing, dan prostatitis dalam 1 bulan terakhir dimasukkan ke dalam kriteria eksklusi. Pada penelitian ini didapatkan 62 sampel. Rentang usia sampel 28-85 tahun dengan proporsi terbanyak pada kelompok usia 58-72 tahun, yaitu sebesar 66,7%. Dari keseluruhan sampel, 41,3% memiliki nilai kadar PSA serum >99ng/dL dan 85,5% memiliki skor Gleason yang buruk. Hasil uji Spearman menunjukkan adanya hubungan yang bermakna (r=0,216; p=0,046) antara kadar PSA serum dan skor Gleason pada pasien kanker prostat di RSUD Dr. Soetomo Surabaya.Â

TRANSPLANTATION OF ALLOGENIC ADIPOSE-DERIVED STEM CELL IMPROVE URETHRAL MUSCLE-COLLAGEN RATIO AFTER VAGINAL DISTENSION Yudiana, I Wayan; Hardjowijoto, Sunaryo; Alif, Sabilal; Purwati, Purwati; Sandhika, Willy; Budiono, Budiono
Indonesian Journal of Urology Vol 21 No 2 (2014)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v21i2.30

Objective:To investigate the effects of allogenic adipose-derived stem cell (ADSC) transplantation on urethral tissue regeneration after vaginal distension in a rat model.Material & Method:32 female wistar rats underwent vaginal distension (VD) for 4 h. Subsequently, PKH-labeled ADSCs (2 x 105 in 200 µl PBS, ADSC group, n = 18) or PBS (200 µl, placebo group, n = 18) were injected into periurethral tissue. Two and four weeks following transplantation (ADSC or PBS), voiding behavior (frequency) and muscle-collagen ratio of urethral tissue were measured to evaluate urethral sphincter regeneration. Data were analysed by paired-t test, one-way ANOVA and post hoc LSD.Results:Mean voiding frequency was significantly different in the ADSC group at two weeks (p<0.0001), but not significantly different at four weeks (p=0.448) when compared with the placebo group.Masson’s trichrome staining revealed that the muscle-collagen ratio of urethral tissue was not significantly different between study groups at two or four weeks (p=0.053 or p=0.166 respectively). Muscle-urethral ratio was more specific showing a significant difference at two weeks (p=0.043). There were significant differences about muscle-collagen or muscle-urethral ratio between control and placebo groups (p<0.05), whereas between control and ADSC groups no significant difference was observed (p>0.05).Conclusion:ADSC transplantation promotes urethral muscle-collagen ratio with development of striated muscle after vaginal distension, so that can improve voiding behavior in a rat model.Keywords:Adipose-derived stem cell, vaginal distension, rat-SUI model, muscle-collagen ratio, voiding stain on paper.

ADIPOSE-DERIVED STEM CELL TRANSPLANTATION ON URETHRAL MICROVESSEL AND VOIDING FUNCTION Mahadi, Endi Prilansa; Hardjowijoto, Sunaryo; Alif, Sabilal; Purwati, Purwati; Sandhika, Willy; Budiono, Budiono
Indonesian Journal of Urology Vol 22 No 1 (2015)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v22i1.101

Objective: To examine the effects of adipose-derived stem cell (ADSC) transplantation on urethral tissue microvessel and voiding function on artificial stress urinary incontinence (SUI) in rats. Material & methods: 25 of 32 female wistar rats underwent vaginal distension as animal-SUI model. ADSCs were isolated from the peri-ovary fat, examined for stem cell properties, and labeled with PKH-2. Seven rats without vaginal distension and periurethral ADSCs injection as control. Twelve rats received periurethral injection of phosphate buffer saline as plasebo and 13 rats received periurethral injection of ADSCs. 14 and 28 days later, voided volume was meassured with voided stained on paper and microvessel density was meassured with immunohistochemical analysis (factor VIII R-Ag). Results: Voided volume analysis showed that rats in the plasebo group had abnormal voided volume compare to normal rats in day 14 (168.5 ± 119.05l vs 621.21 ± 283.85l; p < 0.05), and insignificant improvement of voided volume compare to normal rats in day 28 (852.09 ± 626.7l vs 868.49 ± 578.0l; p > 0.05). While in the ADSCs group only showed significant improvement of voided volume compare to abnormal rats in days 14 (379.35 ± 191.74l vs 228.18 ± 56.26l; p < 0.05). Immunohistochemical analysis showed that microvessel density higher in the ADSCs group compare to plasebo group in days 28 (12.86 ± 2.5 vs 9.50 ± 1.64; p < 0.05). Conclusion: ADSCs transplantation promotes improvements voided volume and urethral microvessel in the rat-SUI model. Keywords: Adipose-derived stem cell, vaginal distension, voided stained on paper, microvessel density.

EFFECT OF PERIURETHRAL INJECTION ADIPOSE-DERIVED STEM CELLS IN NERVE HEALING AND VOIDING BEHAVIOR FOR THE TREATMENT OF STRESS URINARY INCONTINENCE IN A RAT MODEL Rizaldi, Fikri; Purwati; Sandhika, Willy; Budiono; Hardjowijoto, Sunaryo
Indonesian Journal of Urology Vol 28 No 1 (2021)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v28i1.713

Objective: To investigate whether ADSCs are capable of nerve repair in stress urinary incontinence. Material & Methods: Thirty rats were allocated to five groups for receiving a periurethral injection with Phosphate Buffer Saline (PBS) (B1,B2) and Adipose-derived Stem Cells (C1, C2). Functional assessments with Voided Stain on Paper (VSOP) Method were reassessed at 2 and 4 weeks after injection. The rat SUI model was generated by Vaginal Distention (VD). The regeneration of peripheral nerve was evaluated by PGP 9.5 immunohistochemical staining. Results: Results revealed significant peripheral nerve differences between ADSC group and other groups in 28 days using PGP 9.5 staining. Our functional assessment, Voided Stain on Paper (VSOP), showed that ADSC group had significant improvement in 14 days; unfortunately, our 28 days improvement was not substantial. Conclusion: Periurethral injection of ADSCs improves urine pattern in SUI-induced rats and increase the amount of peripheral nerve. This method may be a potential strategy for SUI treatment.

Co-Authors A.A. Ketut Agung Cahyawan W Agung Dwi Wahyu Widodo Agustin Nurliani Alief Yudo Astuti Aries Sasongko Astindari Astindari Astindari Astindari, Astindari Ayu Tyasmara Azarya Ibrahim Baiq Ratna Kumaladewi betty Agustina Tambunan, betty Agustina Brama Rachmantyo Budiono Budipramana, Vicky Sumarki Cita Rosita Sigit Prakoeswa Densy Violina Harnanti Desak Gede Agung Suprabawati Dian Eskaningrum Djoni Susanto Dwi Murtiastutik Endi Prilansa Mahadi Erlina Erlina Faizah, Zakiyatul Fanny Gunawan Firda Azizah Grace Ariani Hartecia, Gilda Heny Arwati Heryawati Heryawati I Wayan Yudiana Ida Septika Wulansari INDROPO AGUSNI Irene Lingkan Parengkuan Iswinarno Doso Saputro iwan Syarif Jimmy Hadi Widjaja Jonan, Bernadetta Junita Jeanne Paliman Kartika Arum Wardani Kholida Nur Aini Kintan Putri LaleMaulin Prihatina Linda Astari M. Yulianto Listiawan Marina Rimadhani Marina Rimadhani Martha Kurnia Kusumawardani Maylita Sari Maylita Sari, Maylita Meita Ardini Pratamasari Muhammad Miftahussurur Nanda Amalia Ramadhanti Navisa, Claudia Clary Novia Indriyani Adisty Nurina Hasanatuludhhiyah Nurul Wiqoyah, Nurul Poernomo, Adinda Sandya Priangga Adi Wiratama PUNGKY MULAWARDHANA, PUNGKY Purwati Purwati Purwati Puteri, Agnes Ilene Suprapto R. Heru Prasetyo R.A. Astrid Putri Wandhita Rahel Yuana Sadikim Ratna Darjanti Haryadi Regina Martina Cilik Rizaldi, Fikri Rulisiana Widodo Rusdamayanti Rusdamayanti Rwahita Satyawati Dharmanta Ryski Meilia Novarina, Ryski Meilia Sabilal Alif Sari, Festi Artika savitri, Lisa Sawitri Sawitri Sawitri Sawitri Soilia Fertilita Sunarso Suyoso Sunaryo Hardjowijoto Tessy Badriyah, Tessy Theresia Fifi Judikristiani Tri Hartini Yuliawati Trisniartami Setyaningrum Tyasmara, Ayu Ummi Maimunah Vinna Chrisdianti Widati Fatmaningrum Wilda Fitria Rachmadina Windya Tri Hapsari Winona May Hendrata Wiratama, Priangga Adi Yusfita Evi Rosdiana

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