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All Journal HAYATI Journal of Biosciences Jurnal Hortikultura Indonesia Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Bumi Lestari Zuriat Jurnal Ilmiah Aplikasi Isotop Radiasi BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Pelita Perkebunan (Coffee and Cocoa Research Journal) AGRIVITA, Journal of Agricultural Science Jurnal Agroekoteknologi Indonesian Journal of Biotechnology Biology, Medicine, & Natural Product Chemistry Bioeksperimen: Jurnal Penelitian Biologi Journal of Tropical Biodiversity and Biotechnology ANNALES BOGORIENSES Jurnal Pertanian Agros Biosaintifika: Journal of Biology & Biology Education Scripta Biologica JURNAL ILMIAH AGRINECA Agrosaintifika: Jurnal Ilmu-Ilmu Pertanian

Endang Semiarti

Fakultas Biologi, Universitas Gadjah Mada, Indonesia

Author-ID : 375592

Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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HOMOLOGI FUNGSI GEN KNAT1 ( Knotted 1– like Arabidopsis thaliana) PADA ANGGREK BULAN Phalaenopsis amabilis (L.) Bl. DENGAN MEDIATOR Agrobacterium tumefaciens Sulastri Isminingsih; Endang Semiarti; Aziz Purwantoro
Jurnal Agroekoteknologi Vol 1, No 1 (2009)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

ABSTRACTTo understand the function of KNAT1 gene Arabidopsis in the forming and developing of Indonesian origin orchid shoot Phalaenopsis amabilis through transform p35S::KNAT1 and pGreen to protocorm like bodies (plb) of the orchid mediated by Agrobacterium tumafaciens LBA4404. The plb transformants were grown on New Phalaenopsis selection medium containing 5 mg/l BAP, 0.15 mg/l NAA, 15 mg.l Kanamycin and addition of 300 mg/l Cefotaxim to eliminate the overgrowth of Agrobacterium. The analysis of positive transformant use Polimerase Chain Reaction (PCR) with the specific oligonucleotide primers for KNAT1 gene: KNAT1F1R1 and universal primers for pGreen: 35SO and Tnos. The result shows that 3 shoots of 1850 transformants positively carry out the 35S::KNAT1 construct (frequency of transformation was 0.16 %) while 5 shoot of 1850 transformants also positively carry out the pGreen vector, with the frequency of transformation was 0.27 %. The phenotype analysis of 35S::KNAT1 transformants show multiplication on forming of the leaf from a plb to + 10 shoots and forming of the leaf shape which has terompet like shapes and rectangular shape.Key words: Arabidopsis, KNAT1 gene, Phalaenopsis amabilis, shoot

PROPAGASI Dendrobium stratiotes Rchb.f. DENGAN BENZILADENIN SECARA IN VITRO Umi Kulsum Nur Qomariah; Endang Semiarti
AGROSAINTIFIKA Vol 1 No 1 (2018): November
Publisher : LPPM Universitas KH. A. Wahab Hasbullah

Tanaman Anggrek Dendrobium stratiotes Rchb.f. merupakan salah satu jenis angggrek alam indonesia. Populasi Dendrobium stratiotes Rchb.f. menurun bahkan terancam punah jika perdagangan terus berlanjut tanpa ada regulasi yang baik. Perbanyakan Dendrobium stratiotes Rchb.f. sangat diperlukan untuk konservasi dan menjaga plasma nutfah.Penelitian ini bertujuan untuk melakukan propagasi Dendrobium stratiotes Rchb.f. dengan BA secara in vitro. Metode penelitian diawali dengan polinasi secara selfing pada bunga Dendrobium stratiotes Rchb.f. Biji Dendrobium stratiotes Rchb.f. umur 4 bulan hasil polinasi ditumbuhkan secara in vitro selama 6 bulan sampai menjadi tuas yang diap diinduksi dengan kriteria memiliki 2-3 daun dan 1-2 akar. Induksi dilakukan pada media ½ KC dan ½ MS yang masing-masing diberi perlakuan BA 1 PPM, 5 PPM dan 9 PPM. Kultur diinkubasi pada suhu 25±20C dengan fotoperiodisasi 16 jam terang dan 8 jam gelap. Parameter yang diamati meliputi warna daun, jumlah tunas, jumlah daun, panjang daun, jumlah akar dan panjang akar. Hasil penelitian menunjukkan bahwa Perlakuan BA 9 PPM pada media ½ MS menghasilkan jumlah tunas adventif terbanyak dibandingkan semua perlakuan dan berbeda nyata. Analisis Uji F pada α 0,05 menunjukkan pengaruh BA terhadap jumlah tunas adventif, berbanding lurus terhadap taraf konsentrasi BA. Kombinasi perlakuan BA pada media ½ MSmenghasilkan multiplikasi jumlah tunas adventif plantlet Dendrobium stratiotes Rchb.f. terbanyak dibanding kombinasi perlakuan lainnya. Dengan demikian maka propagasi dalam rangka konservasi Dendrobium stratiotes Rchb.f. dapat dilakukan melalui aplikasi BA pada media ½ MS secara in vitro yang ditunjukkan dengan terbentuknya tunas adventif.

INDUKSI PEMBUNGAAN IN VITRO PADA ANGGREK BULAN Phalaenopsis amabilis (L.) Blume INDONESIA IN VITRO FLOWERING OF INDONESIAN Phalaenopsis amabilis (L.) Blume Ixora Sartika Mercuriani; Agus Slamet; Bekti Sulistya Utami; Aries Bagus Sasongko; Aziz Purwantoro; Sukarti Moeljopawiro; Endang Semiarti
Agros Journal of Agriculture Science Vol 16, No 2: Edisi Juli 2014
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.529 KB)

Bunga adalah faktor yang sangat penting dalam budidaya anggrek. Salah satu kendala yang sering dijumpai dalam budidaya anggrek adalah lama fase vegetatif yang dibutuhkan tanaman tersebut untuk dapat berbunga. Pada penelitian ini induksi pembungaan dilakukan pada tahap pertumbuhan awal tanaman secara in vitro. Tanaman umur enam bulan setelah tanam (bst) disubkultur pada medium New Phalaenopsis (NP) dengan konsentrasi Nitrogen (N) setengah dari NP (1/2NP), dengan atau tanpa pemberian Benzyl Adenine (BA), variasi konsentrasi KH2PO4 (1,5 mM dan 3 mM), serta dengan atau tanpa pemotongan akar. Kombinasi perlakuan dengan pemberian BA 22.2 µM, kandungan P tinggi (3 mM), dan pemotongan akar mampu mempercepat pembungaan in vitro anggrek Phalaenopsis amabilis (P. amabilis) asli Indonesia pada umur 18 bst.Flowering is very important in orchid cultivation. However, the long vegetative phase to be able to bloom of the plant becomes an important problem. The orchid needs three up to five years after sowing to bloom. In this study, flowering induction is done in the early growth stages of plants. At six months after sowing (mas), plants were sub-cultured on New Phalaenopsis (NP) medium witha half Nitrogen(N) concentration of NP (1/2NP), with or without Benzyl Adenine (BA), concentration variations of Phosphor/P (1,5 mM and 3 mM), and with or without roots cutting. In vitro flowering of Indonesian Phalaenopsisamabilis (P. amabilis) can induced on medium that contain 22.2 µM BA and 3 mM P with roots cutting at 18 mas.

KARAKTERISTIK ISOLAT Rhizoctonia sp. PATOGENIK DAN Rhizoctonia MIKORIZA PADA TANAMAN ANGGREK TANAH (Spathoglottis plicata) S. Soelistijono; Christanti Sumardiyono; Achmadi Priyatmojo; Endang Semiarti
JURNAL ILMIAH AGRINECA Vol. 12 No. 1 (2012): JURNAL AGRINECA
Publisher : Fakultas Pertanian, Universitas Tunas Pembangunan Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36728/afp.v12i1.497

Mycorrhizal Rhizoctonia is a fungus that capable to associate with terrestrial orchids. Apart from being mycorrhizal, there are isolates of Rhizoctonia sp. that are pathogenic and caused root rot disease on Spathoglottis plicata. This study aimed to know the differences between pathogenic Rhizoctonia sp. and mycorrhizal Rhizoctonia in morphology and molecular structure using RAPD technique. The results showed that colony colour, Â cell lenght and nucleus number a several isolates of pathogenic Rhizoctonia sp. and of mycorrhizal Rhizoctonia on S. plicata had no differences, but had differences on cell thickness and isolate grouping based on hyphal anastomosis test. RAPD molecular technique showed that each isolate of pathogenic Rhizoctonia sp. and mycorrhizal Rhizoctonia had differences on DNA structure.

PENGARUH PEPTON TERHADAP PERTUMBUHAN EMBRIO ANGGREK Vanda tricolor Lindley var. Suasiv ASAL MERAPI SECARA IN VITRO Rizka Riliant Puspasari; Ikhsanudin Nur Rosyidi; Eka Fitriana Candra Ningrum; Endang Semiarti
Scripta Biologica Vol 5, No 1 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Orchidaceae is a valuable ornamental plant in Indonesia’s export commodities. The growth of orchid in nature is prolonged due to its microscopic size of the seed and has no endosperm. In the mass-production, an inducer is required to accelerate the growth in an in-vitro culture, one of which is peptone. The embryos used in this study is Merapi endemic orchid, the Vanda tricolor Lindley var. Suavis. This study aimed to determine the effect of peptone on the growth of eight weeks old of Vanda tricolor Lindley var. Suavis embryos and to determine the optimal peptone concentration to induce the growth of the orchid embryo. This experiment was conducted in the Biotechnology Laboratory of the Faculty of Biology Universitas Gadjah Mada from September to October 2016. The treatments applied in this study was a variety of peptone concentrations (0–3 g/L) in growth media to induce eight weeks old protocorm subcultures. The study measured the morphological development of protocorm to determine the effect. The results showed a positive effect of peptone on the growth of orchid embryos at the NP1 treatment concentration of 1 g/L. The growth reached the phase 6 with the rate measured about 6.85±0.001% in the fourth week. This value was higher than the protocorm growth rate when it reached the phase 6 in the fourth week on the NP0 medium measured at 5.22±0.003%, on the medium NP2 at 1.15±0.002%, and on the medium NP3 at 1.02±0.001%. This result showed the effect of peptone concentration to induce the growth of Vanda tricolor Lindley var. Suavis embryo, but the excess concentration of peptone inhibited the growth of protocorm.

Morphological And Biochemical Responses Of Saccharum Spontaneum L. Accessions To Drought Stress Aminatun Munawarti; Taryono Taryono; Endang Semiarti; Sismindari Sismindari
Journal of Tropical Life Science Vol. 4 No. 1 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Glagah (Saccharum spontaneum L.) has potential value as a crop species and may also be used in sugarcane breeding programs; however, this germplasm has not been extensively used in breeding programs, primarily in relation to improve drought tolerance. The objectives of this experiment were to evaluate the effect of drought stress initiated at vegetative growth stage on growth, leaf proline content and protein pattern of seven glagah accessions (BOT-53, BOT-54, BOT-60, BOT-77, BOT-78, BOT-84, and BOT-88). The plants were propagated from single node stalk segments in polybag in the field under non-stress condition for two months. The two month-old plants were then subjected to drought stress by withholding watering for eight weeks. Untreated control plants were watered every two days. Results indicated that drought stress reduced plant height, stalk diameter and green leaf number. On the other hand, there was a little difference between drought-stressed and control plants in terms of proline content. The protein pattern showed that drought stress caused a change in gene expression in the form of induction or repression of protein expression. A specific protein with a low range of molecular weight (Rf value about 0.647) showed constitutively expressed in accession BOT-53 but drought-inducible expressed in BOT-54. Keywords: Drought stress, glagah, proline, protein pattern, Saccharum spontaneum

Biodiversitas dan Kekerabatan Fenetik Spesies Anggrek Alam di Kawasan Ekowisata Ayunan Langit, Kulonprogo Evilili Usmanti; Febri Yuda Kurniawan; Maura Indria Meidianing; Amru Rizal Basri; Endang Semiarti
Al-Kauniyah: Jurnal Biologi Vol 15, No 2 (2022): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v15i2.19801

AbstrakEkowisata Ayunan Langit memiliki kondisi geografis yang mendukung bagi pertumbuhan anggrek, sehingga daerah tersebut memiliki diversitas anggrek alam yang tinggi sebagai salah satu daya tarik wisata. Beberapa spesies anggrek dapat memiliki kemiripan secara morfologi meskipun tidak berkerabat dekat. Dalam rangka pengembangan Kawasan Wisata Terpadu Ayunan Langit diperlukan data keragaman spesies-spesies anggrek di kawasan ini. Penelitian ini bertujuan untuk melakukan inventarisasi, identifikasi, dan melihat hubungan kekerabatan antar spesies anggrek alam di Kawasan Ayunan Langit. Metode yang digunakan dalam penelitian ini adalah koleksi spesimen, identifikasi, dan karakterisasi morfologi organ vegetatif spesimen, serta analisis hubungan kekerabatan. Analisis kekerabatan menggunakan software Ms Excel dan MVSP. Terdapat 14 spesies anggrek alam yang ditemukan di Kawasan Ayunan Langit. Anggrek alam di kawasan tersebut didominasi dari anggota Subfamili Epidendroideae dan anggrek epifit. Semua spesies masuk dalam kategori Appendix II CITES. Berdasarkan analisis kekerabatan fenetik, dendogram terbagi dalam dua kluster berdasarkan karakter morfologi organ vegetatifnya. Spesies anggrek yang berasal dari genus yang sama belum tentu dapat mengelompok pada klaster yang sama, misalnya pada Dendrobium. Karakter morfologi organ vegetatif belum dapat digunakan untuk melihat hubungan kekerabatan fenetik secara akurat pada anggrek. Karakter organ generatif berperan penting dalam memberikan hasil visualisasi kekerabatan antar spesies anggrek yang lebih akurat.AbstractAyunan Langit ecotourism has favorable geographical conditions for orchid growth, hence the area has a high diversity of wild orchids as a tourist attraction. Several orchid species can be morphologically similar even though they are not closely related. For Ayunan Langit integrated tourism area development, orchid diversity data are required. This study aimed to conduct an inventory, identification, and relationship analysis between wild orchid species in Ayunan Langit ecotourism. Specimen collection, identification, and morphological characterization were conducted on specimen's vegetative organs. Relationship analysis was conducted with Ms Excel and MVSP. There are 14 wild orchid species found in the Ayunan Langit, dominated by Epidendroideae members and epiphytic orchids. All species are included in the CITES Appendix II category. Based on the phenetic relationship analysis, the dendrogram was divided into two clusters based on the morphological characters of the vegetative organs. Orchid species belongs to the same genus may not necessarily be grouped in the same cluster, for example in Dendrobium. Morphological characters of vegetative organs cannot be used to visualize the phenetic relationship accurately in orchids. Generative organ characters play an important role in providing a more accurate visualization of the relationship between orchid species.

Establishment of an efficient primary callus induction for embryogenic potential of Coffea arabica Rina Arimarsetiowati; Budi Setiadi Daryono; Yohana Theresia Maria Astuti; Endang Semiarti
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 38 No 2 (2022)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v38i2.510

Coffea arabica L. is a species of coffee that contribute for more than seventy percent of world coffee production. Various attempts have been made to obtain large quantities of planting material through propagation in vitro somatic embryogenesis technology. The objective of this experiment was to evaluate the effect of different plant growth regulators (PGRs) on callus induction (indirect somatic embryogenesis) in AS2K clone of Arabica coffee. Mother plants of Arabica coffee were established in coffee experimental field of Indonesian Coffee and Cocoa Research Institute at Andung Sari, Bondowoso, East Java, Indonesia (-7˚55'' ' S, 113˚41'' ' E) at an altitude of 1380,1 m dpl. Leaf explants were cultured on a half-strength Murashige and Skoog (MS) medium supplemented with various concentration (1.0, 2.0, 3.0 mg/L) of 2,4-D and (1.0, 2.0, 3.0 mg/L) thidiazuron in combination with 1.0 mg/L BAP. All the experiments were organized in completely random design (CDR) and repeated three times, each using minimum seven replicates (a total of 21 explants per treatment). The morphologycal and histological analysis of the different types of callus were observed. The percentage of callus formation was recorded every two weeks until eight weeks. The highest percentage of callus formation (almost 60%) was in medium containing 1 mg/L 2,4-D dan 1 mg/L BAP. Morphological and histological studies prove that the callus has a friable and embryogenic texture and begins to develop various stages of somatic embryo formation, starting with the globular, heart, torpedo and cotyledonary phases.

Co-Authors , Surifah A.A. Ketut Agung Cahyawan W Achmadi Priyatmojo Aditya Nur Subchan Agus Slamet Ahmad Suyoko Alisa Julia Nurulita Amarilis Andani Kesuma Aminatun Munawarti Aminatun Munawarti, Aminatun Amru Rizal Basri Anami Riastri Annisa, RR Rifka Ari Indrianto Ari Indrianto Ari Indrianto Ari Indrianto Ari Indrianto Ari Indrianto Ari Indrianto Aries Bagus Sasongko Asri Fajar Milasari Azis Purwantoro Azis Purwantoro Aziz Purwantoro Bekti Sulistya Utami Binti Tsulsiyah Brilliant Kharisma Apritadila Budi Setiadi Daryono BUDI SETIADI DARYONO Cahya Lembayung Sutra Catharina Tri Widyastuti Christanti Sumardiyono Christanti Sumardiyono Della Rosiana Ningtias Devi Bunga Pagalla Dewi Tika Sari Dewi Yuliana Rizqi Dika Sundari Eka Fitriana Candra Ningrum Evilili Usmanti Exsyupransia Mursyanti Exsyupransia Mursyanti Exsyupransia Mursyanti Faiza Senja Widya Perdana Falah Nur Alifianto Febri Yuda Kurniawan Fhea Putri Cristy Fitriana Puspitasari Frisca Damayanti Gde Cahyadi Wirajagat Ikhsanudin Nur Rosyidi Ixora Sartika Mercuriani JAKA WIDADA Jose Gutierrez-Marcos Jose Gutierrez-Marcos Kana Ninomiya Maryani Maryani Maura Indria Meidianing Muhammad Dylan Lawrie Muhammad Dylan Lawrie Muhammad Dylan Lawrie Muhammad Dylan Lawrie Naufal Ghozi Aditya Perdana Ni Luh Putu Kayika Febryanti Nintya Setiari Nintya Setiari Oedjijono Oedjijono Oktaviana Herawati Putra, Rinaldi Rizal R Soelistijono Rahayu Sulistianingsih Rina Arimarsetiowati Rinaldi Rizal Putra, Rinaldi Rizal Rindang Dwiyani Rizka Riliant Puspasari Rumiyati Rumiyati Rumiyati Rumiyati Seonghoe Jang Setiaji, Arkan Shogo Matsumoto Shogo Matsumoto Sismindari . Sismindari Sismindari Sismindari Sismindari Sitarina Widyarani Soenghoe Jang Sri Nopitasari Sri Nopitasari Sri Wahyuningsih Stalis Norma Ethica Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sukarti Moeljopawiro Sulastri Isminingsih Taryono, Taryono Thoyibatul Farida Tri Joko Raharjo Triono Bagus Saputro, Triono Bagus Umi Kulsum Nur Qomariah Windi Mose Windi Mose Woerjono Mangoendidjojo Woro Anindito Sri Tunjung Yasushi Yoshioka Yasushi Yoshioka Yohana Theresia Maria Astuti Yuli Setiawati Yuuki Asano Zulfa Layina

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