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EVALUASI KEAMANAN SUMBER AIR MINUM DESA MOJO KECAMATAN PADANG KABUPATEN LUMAJANG Aditya Oktavianto; Nurhayati Nurhayati; Enny Suswati
JURNAL AGROTEKNOLOGI Vol 8 No 02 (2014)
Publisher : Faculty of Agricultural Technology, University of Jember

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Abstract

Little known about the safety of natural water on enteric mikroflora population at Mojo Village Padang Distric Lumajang Regency. We assessed the population of enteric microflora i.e Salmonella and Eschericia coli in the wellspring. We performed a randomized sampling to evaluate the population of Salmonella and Eschericia coli of three natural water resource at Mojo Village. There were Jirun, Kali Tengah and Sumber Suko water resource. Medium to colony identification were used Salmonella Chromogenic Agar (SCA) and Enteric Hektoin Agar (HEA). SCA resulted blue colony for Eschericia coli and magentha or violet colony for Salmonella. While HEA resulted orange colony for Eschericia coli and green for Salmonella. 250 mL of natural water was used to growth the enteric microflora. We observed three water resource of Mojo Village contain enteric microflora. The enteric microflora population was up to 2 log10 CFU/mL (more than 10 CFU/mL). These was a fantastic population, because enteric population must no growth on drink water. Assuming the enteric microflora come from fish faecal, because at the surrounding of water resource growth the fish. Three water resource at Mojo Village were no safety to used as drink water if without water treatment. The enteric microflora can dextructed by using filtration and UV treatment or thermal process example water boiling.Keywords: Jirun, Kali Tengah, Sumber Suko, Salmonella, Eschericia coli, water resource
The 35,2 kda Hemaglutinin Protein of Pili’s Proteus mirabilis P355 as Adhesin on Rabbit’s Blader Epitelial Diana Chusna Mufida; Enny Suswati
Jurnal ILMU DASAR Vol 8 No 1 (2007)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

Urinary tract infection represent one of nosocomial infection in hospital. One of agent nosocomial urinary tract infection is Proteus mirabilis bacteria and common occur on patient with urinary catheter. Urinary tract infection with caused by P. mirabilis was persistent, very difficult to eradicated. Further more is caused some complication such as cystitis, acut and cronic pyelonephritis, kidney bladder stone, bacterimiae and sepsis. This bacteriae has same virulence factors. Fimbriae is one of it. In the adhesion test utilization of protein hemaglutinin pili 35.2 kDa resulted in the electroelusion which salluted in vesica urinaria epitelial by dose 400μl , 200μl, 100μl, 50μl, 25μl, 12,5μl and 0μl as control. Form adhesion test on vesica urinary epitelial that salluted with 35.2kDa protein pili we faund that the higher dose of protein can decrease bacteriae consentration at vesica urinary epitelial. This result was significant with r= 0.93 and p value = 0.005. This conclusion of this study is 35.2 kDa molecular weight protein pili of P. mirabilis P355 were adhesion protein.Keywords : hemaglutinin, pili, Proteus mirabilis, adhesin
The Role of Pili Protein 38,6 kDa Klebsiella pneumoniae as a Hemagglutinin and Adhesin Protein which Serves as a Virulence Factor Regina Finka; Dini Agustina; Dwita Aryadina Rachmawati; Enny Suswati; Diana Chusna Mufida; Ali Shodikin
Journal of Agromedicine and Medical Sciences Vol 5 No 2 (2019)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v5i2.9558

Abstract

Klebsiella pneumoniae is an infectious bacteria in various parts of the body. The presence of proteins in pili that will bind to cell surface receptors (adhesin proteins) and cell membrane sugar molecules (hemagglutinin proteins) is a very influential factor in the ability of K. pneumoniae to enter the human body. The purpose of this study was to determine the role of pili 38.6 kDa K. pneumoniae protein as a hemagglutinin and adhesin protein which functions as a virulence factor. This type of research is pure experimental research using the electrophoresis method (SDS-PAGE) to obtain protein. The protein obtained was then tested for hemagglutination and adhesion test using erythrocyte cells and enterocytes of BALB / C mice to determine their role as hemagglutinin and adhesin proteins. Conclusion of this study pili protein 38.6 kDa Klebsiella pneumoniae acts as a hemagglutinin and adhesion protein which functions as a virulence factor. Keywords: Klebsiella pneumonia, 38,6 kDa Protein, Hemagglutinin, Adhesin
Tetraciclyne Resistance Eschericia coli Isolated From Broiler Chicken Meat Anisa Rizca Putri; Enny Suswati; Laksmi Indreswari
Journal of Agromedicine and Medical Sciences Vol 4 No 1 (2018)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v4i1.6402

Abstract

Antibiotic resistance is a serious health problem that have ben uneffective therapy. The cause of antibiotic resistance 80% comes from food of animal origin such as Broiler Chicken. E. coli contamination in antibiotic resistant chicken has been shown to transfer genetic factors between bacteria in the human intestinal system. Almost all broiler breeders use commercial feed containing tetracycline antibiotics. The use of antibiotics in the feed mixture is one of the risk factors for resistance. The aim of this study was to get an information of antibiotic resistance E. coli which isolated from broiler meat. Identification test of E. coli used two phase, presumtive test and confirmed test. Sensitivity test for E. coli to antibiotic by disc diffusion Kirby Bauer method. This study used 6 sample of upper thigh broiler meat, the antibiotic use tetracycline. The data were analyzed descriptively. The results of this study show that from 6 isolate sample, there are 4 isolate identificated as E. coli and 2 isolate show resistance to the tetracycline antibiotic. The conclusion of this study showed resistance has occurred on 50% sample.
Difference of Right Ventricular Systolic Function Before and After Hemodialysis in Chronic Kidney Disease Stage V In RSD dr. Soebandi Jember Saskia Mediawati; Suryono Suryono; Enny Suswati
Journal of Agromedicine and Medical Sciences Vol 4 No 2 (2018)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v4i2.7873

Abstract

End stage renal disease is a clinical state with an irreversible chronic decreasing function of renal that need a hemodialysis or renal transplant as theraphy. One of the complication of End stage renal disease is cardiovascular. Cardiovascular complication is a major cause of mortality and mobility in End stage renal disease by 44% in Indonesia. Systolic function of right ventricle can be used to assess the heart function. The purpose of this study is to differentiate the systolic function of right ventricle in patient with end stage renal disease in RSD dr. Soebandi Jember. We used analytical observational approach with cross sectional method held at Dialysis and Echocardiography Unit of RSD dr. Soebandi Jember from September to November 2017. Thirty patients with end stage renal disease who routinely undergoes dialysis were observed. Shapiro-Wilk test results 0,000 significance shows that the data is not normally distribute. Wilcoxon test results significance level 0,005 which shows there is a differentiation between before and after hemodialysis of systolic function in end stage renal disease patients of RSD dr. Soebandi Jember. Keywords : End stage renal disease, systolic function on right ventricle, hemodialysis
The Effectiveness of Edamame Seed (Glycine max L. Merril) Ethanolic Extract to Fibroblast Count on Second Degree Burn Wound Healing Arifah Nur Hasanah; Ika Rahmawati Sutejo; Enny Suswati
Journal of Agromedicine and Medical Sciences Vol 5 No 3 (2019)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v5i3.6831

Abstract

Burn wound healing process consists of three phases of inflammation, proliferation and remodelling. The number of fibroblasts has increased during the proliferation phase. Oxidative stress in burn may disrupt proliferation through TGF-β inhibition, result in decreased number of fibroblasts and poor burn wound healing. Genistein in edamame seeds has anti-inflammatory, antibacterial and antioxidant properties. Edamame contains vitamins A, C, and E which is also have antioxidant properties. Edamame has been suggested to protect burn wound healing from oxidative stress. This study aimed to understand the effectiveness of edamame seed (Glycine max L. Merril) ethanolic extract (ED) to fibroblast count in second degree burn wound healing. This is true experimental research with post test only control group design. The study was conducted at University of Jember during November until December 2017. The sample of this research was 24 rat models of second degree burn which was made by attaching hot metal (60 ° C) for 5 seconds on the shaved back of the rats. The sample was divided into 6 groups with topical treatment, among others: positive control group (silver sulfadiazine), negative control group (Na CMC 0.5%), group with ED 20%, ED 40%, ED 60%, and ED 80%. Treatment was given for 15 days and skin sample was taken on the 16th day. Observation of the number of fibroblasts was done by taking pictures of histhologic preparations in Hematoxylin and Eosin staining under 400x magnification of light microscope. The number of fibroblast of each picture was processed using Image-J software. In one way ANOVA test, significance value of 0.011 (p≤0,05) indicated that there was at least a significant difference between 2 groups. LSD’s post hoc analysis showed that there was a significant difference in the group ED 20, 40, and 60% compared with negative control. Keyword: burn wound healing, vegetable soybean, genistein, fibroblast count, silver sulfadiazine
The Effect of Mirabilis jalapa Leaf Ethanolic Extract against Streptococcus pyogenes Bagus Satrio Pambudi; Enny Suswati; Jauhar Firdaus
Journal of Agromedicine and Medical Sciences Vol 2 No 1 (2016)
Publisher : Faculty of Medicine, University of Jember

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Abstract

Streptococcus pyogenes is a Gram positive bacteria that commonly cause disease in human. If not treated immediately, this bacteria can cause serious complication such as reumatic fever that causing heart valve tissue damage. Penicilin, drug of choice to eradicate S. pyogenes, oftenly cause various side effects such as anaphylaxis. This study aimed to investigate the effect of Mirabilis jalapa leaf ethanolic extract against S. pyogenes growth using in vitro techniques. The study design was a quasi experimental design. S. pyogenes culture as the study subject were divided into positive control group (penicilin V 100 IU), negative control group (NaCMC 0,5%), and eight treatment groups that were given with M. jalapa leaf ethanolic extract as much as 0,1 mg/ml, 1 mg/ml, 5 mg/ml, 10 mg/ml, 20 mg/ml, 30 mg/ml, 40mg/ml, and 50 mg/ml. After 24 hours incubation periods, the inhibition zone were found in all treatment group except in concentration 0,1 mg/ml. This study showed that M. jalapa leaf ethanolic extract could inhibit the growth of S. pyogenes. There was significant correlation between the concentration of M. jalapa leaf ethanolic extract and the diameter of inhibition zone (p=0,00), the higher concentration of M. jalapa leaf extract, the larger diameter of inhibition zone of S. pyogenes. Keywords: Mirabilis jalapa, leaf extract, antimicrobial activity
The Association between Environmental Sanitation and Soil Transmitted Helminths (STH) Coinfection of Tuberculosis Patients in Panti District, Jember Regency Nita Alfianti; Yunita Armiyanti; Bagus Hermansyah; Enny Suswati; Dini Agustina; Diana Chusna Mufida
Journal of Ners and Midwifery Vol 7, No 3 (2020)
Publisher : STIKes Patria Husada Blitar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26699/jnk.v7i3.ART.p354-361

Abstract

Tuberculosis (TB) is a contagious infectious disease caused by the bacteria Mycobacterium tuberculosis. More than 80% of TB cases attack the pulmonary organs and the rest are extra-pulmonary TB. Indonesia is one of the countries with the high number of tuberculosis cases besides India, China, the Philippines, and Pakistan. Aside from being a country with a high TB incidence, Indonesia is also an endemic country for helminth infections, especially Soil-Transmitted Helminths (STH). The high prevalence of STH infections in Indonesia allows STH coinfection in TB patients to cause decreasing immunity, thus affecting the outcome of TB infection. STH infection is very closely related to environmental sanitation. The purpose of this study was to identify and determine the association of environmental sanitation risk factors to the coinfection of Soil-Transmitted Helminths (STH) in tuberculosis patients in Panti District, Jember Regency. This type of research was observational research with cross-sectional analytic design. Of the 49 TB patients who were undergoing treatment at the Panti Health Center, 32 people were willing to be respondents, but only 25 people collected stool samples. Fisher's test results showed that the incidence of intestinal co-infection in TB patients in Panti District, Jember Regency was 12% and was caused by two STH species, namely A. lumbricoides and Hookworm. Environmental sanitation in TB patients was mostly good, e.g 54.5% of respondents had good environmental sanitation, but there was no significant association between environmental sanitation and the incidence of intestinal co-infection in TB patients in Panti District, Jember Regency (p> 0.05).
The Association Between Patient Characteristics and Disease Outcomes of COVID-19 in Jember, Indonesia Diana Chusna Mufida; Putu Ayu Laksmi Lestari; Azham Purwandhono; Enny Suswati; Angga Mardro Raharjo
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 15 (2021): Special Category COVID-19
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33533/jpm.v15i0.3441

Abstract

COVID-19 can cause serious conditions in both comorbid and elderly patients; hence, they need to be given proper follow-up treatment. This study aimed to determine the association between patient characteristics and disease outcomes of COVID-19 in Jember, Indonesia. This research is an analytic observational study with cross-sectional approach. The sample was 304 patients who were confirmed positive for COVID-19 at the COVID-19 referral hospitals in Jember. This study used medical record data analysed by using both univariate and bivariate tests. Patient characteristics related to the disease outcomes in patients who were positive for COVID-19 in Jember, are as follows: age (p = 0.000), clinical symptoms (p = 0.017), comorbidities (p = 0.000), and complications (p = 0.000). The unrelated variables were sex (p = 0.455), education level (p = 1.000), domicile (p = 1.000), occupation (p = 0.322), and smoking history (p = 0.147). Patients most at risk of developing fatal outcomes are those with complications. Patient characteristics associated with the disease outcomes in patients positive for COVID-19 were age, clinical symptoms, comorbidities, and complications. While, those that were not related to the disease outcomes in patients were sex, education level, occupation, domicile, and smoking history.
The Difference of Eosinophil Amount in Tuberculosis Patients with and without Soil-Transmitted Helminths Co-infection in Panti District, Jember Yunita Armiyanti; Nisrina Salsabila Firmansyah; Angga Mardro Raharjo; Muhammad Ali Shodikin; Enny Suswati; Dini Agustina
NurseLine Journal Vol 5 No 1 (2020): May 2020
Publisher : Faculty of Nursing, Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/nlj.v5i1.16729

Abstract

Tuberculosis is one of the world's health problems even in Indonesia. The immune response that needed to deal with TB can be influenced by other infections, such as helminth infection. The helminth infections induce Th2 immune responses and eosinophilia to eliminate these pathogen. Meanwhile, the Th2 response and eosinophilia can also suppress the Th1 immune response which is very important to eliminate the bacterium M. tuberculosis and make a negative impact on the success of TB therapy. Thus, the eosinophil profile can describe the immune response in TB patients with helminth co-infection. This study aims to determine the prevalence of helminthiasis in TB patients and determine the differences in eosinophil amount in tuberculosis patients with and without helminth co-infection. This study was an observational analytic study with a cross-sectional design conducted at Puskesmas Panti in August 2019-January 2020 using fecal and blood samples from 24 research subjects. Helminth co-infection status was obtained from fecal examination with sedimentation and flotation methods while the number of eosinophils was obtained from leukocyte counts. Data analysis used the Mann Whitney U test to determine differences in the number of eosinophils in the two groups. The results showed there was no difference in the number of eosinophils in TB patients with helminth co-infection and without helminth co-infection (p> 0.05). There is no typical picture of the number of eosinophils in both groups so that eosinophils cannot describe the immune response that arose in TB patients with helminth co-infections in Panti district, Jember.
Co-Authors Abu Khoiri Aditya Oktavianto Ahmad Hasbi Al-Muzaky Ahmad Hasbi Al-Muzaky Alvien Zahrotun Nadhiva Angga Mardro Raharjo Anisa Rizca Putri APRI KUNTARININGSIH Arifah Nur Hasanah Ariska Nur Aida asihanti rosita ferdiana Azham Purwandhono Bagus Hermansyah Bagus Hermansyah Bagus Hermansyah Bagus Satrio Pambudi Bagus Satrio Pambudi, Bagus Satrio Bima Setia Sandya Nugraha Cholis Abrori, Cholis Desie D. Wisudanti Desie Dwi Wisudanti, Desie Dwi Devi Chintya Kumalasari Dian H. Purnamasari Diana C Diana C. Mufida Diana Chusna Mufida Dini Agustina Dwita Aryadina Dwita Aryadina Rachmawati Elly N Sakinah Elly Nurus Sakinah, Elly Nurus Elvia Rahmi Marga Putri Erfan Efendi Habib Mustofa I Ketut Sudiana Ika Rahmawati Sutejo Indra Samudra Rahmat Indra Samudra Rahmat Jauhar Firdaus Joko Mariyono Kirana Nadyatara Kristianningrum Dian Sofiana Lailatis Shofia Laksmi Indreswari Laylatul Fitriyah Mukarromah M. Ali Shodikin Misnawi Misnawi Misnawi Misnawi Mufida Mufida Muhammad A. Shodikin Muhammad Alif Taryafi Nexia Nevarachell Onny Amirsyah Nindya Shinta Rumastika Nisrina Salsabila Firmansyah Nita Alfianti NUR K. AGUSTIN Nurhayati Nurhayati Prayoga Adinawer Sirait Probosutiksna, Nuno Febrian Probosutiksna, Nuno Febrian Putu Ayu Laksmi Lestari Regina Finka Risty Pradana Linggan Wangi Salsabila, Yuna Annisa Saskia Mediawati septa surya wahyudi, septa surya Shofia, Lailatis Siti Ananda Hardita Syahputri Supangat Supangat Supangat Supangat Suryono Suryono Ulfa Elfiah Wahyuning Ati Ashari Wiwien Sugih Utami Wiwin Sugih Utami Yudha Nurdian Yudha Nurdian Yuna Annisa Salsabila Yunita Armiyanti Yunita Armiyanti Yunita Wulansari