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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia AGRIVITA, Journal of Agricultural Science Jurnal Agroteknos Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat PROSIDING SEMINAR NASIONAL AGRIBISNIS CROP AGRO, Scientific Journal of Agronomy Jurnal Agercolere Berkala Penelitian Agronomi Jurnal Agrium Anoa : Jurnal Pengabdian Masyarakat Sosial, Politik, Budaya, Hukum. Ekonomi Jurnal Pengabdian Masyarakat Ilmu Terapan (JPMIT) Jurnal Pengabdian Nusantara (JPN)
Teguh Wijayanto
Universitas Halu Oleo
Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Electrical & Electronics Engineering Environmental Science Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Social Sciences Other

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RESPON HASIL DAN JUMLAH BIJI BUAH SEMANGKA (Citrullus vulgaris) DENGAN APLIKASI HORMON GIBERELIN (GA3) Teguh Wijayanto; Wa Ode Rahzia Yani; Made Widana Arsana
Jurnal Agroteknos Vol 2, No 1 (2012)
Publisher : Fakultas Pertanian Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56189/ja.v2i1.2262

Abstract

Hormone gibberellin (GA3) is known to be able to increase yield and plays a role in the production of seedless fruits. This research was conducted to determine the effects of GA3 application on yield and quality (seed number) of watermelon. The research was performed at the experimental farm of Agriculture Faculty, University of Halu Oleo.  The research was arranged in a Complete Randomized Design (CRD) with a single factor (dose of GA3), consisted of 6 (six) levels, namely:  without GA3 application (G0), GA3 100 mg L-1 (G1),  GA3 150 mg L-1 (G2),  GA3 200 mg L-1 (G3),  GA3 250 mg L-1 (G4), and GA3 300 mg L-1  (G5).  Each treatment consisted of 4 (four) plants, with 3 replicates, to make an averall 72 experimental units (plants).   Research results showed that the application of GA3 significantly influenced the fresh fruit weight, fruit diameter, fuit content diameter, and seed number.   Treatment of gibberellin 300 mg L-1 (G5) tended to produce higher yield (fresh fruit weight, fruit diameter, fuit content diameter), while G4 treatment (250 mg L-1) resulted in the lowest number of seeds in the fruit.   Despite further research is still needed, this research indicates that the application of gibberellins (GA3) has the potency in increasing the yield of watermelon and improving the quality, in term of the possibility to produce seedless watermelon. Keywords: gibberellins (GA3), fruit yield and quality, seedless fruit, watermelon.
HUBUNGAN KEKERABATAN AKSESI PISANG KEPOK (Musa paradisiaca Formatypica) DI KABUPATEN MUNA BERDASARKAN KARAKTER MORFOLOGI DAN PENANDA RAPD Teguh Wijayanto; Dirvamena Boer; La Ente
Jurnal Agroteknos Vol 3, No 3 (2013)
Publisher : Fakultas Pertanian Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56189/ja.v3i3.2325

Abstract

Twenty-four accessions that belong to four groups of kepok banana in Muna Regency have   been   analyzed   for   their   genetic   diversity   based   on   morphological   characters (qualitative and quantitative characters), and a few accessions based on RAPD markers. This study aimed to determine the genetic diversity and phylogenetic relationship of accessions of kepok bananas based on 52 qualitative and 12 quantitative morphological characteristics and DNA characteristics.   Results of clustering analysis showed the euclidian values ranged between 0.50 to 1.00 for the qualitative data, 0.01 to 0.50 for quantitative data, and 0.83 to 0.88  for  DNA  profile  data.  Combined  qualitative  and  quantitative  data  had  similarity coefficient ranged from 0.00 to 2.50.  Dendogram of each character produced 2 main groups. The main group 1  formed subgroups. Although the qualitative and quantitative characters resulted in different accession   groupings, the combined data analysis of quantitative and qualitative  data  showed  that kepok  banana  in  Muna  regency  was  classified into    4  sub groups namely banana Manuru, Bugisi, Jiwaka and Manuru Lakabu. Keywords:   cluster   analysis,   kepok   banana,   qualitative   and   quantitative   characters, morphology, RAPD markers.
PROSPEK PENERAPAN BIOTEKNOLOGI DALAM PEMANFAATAN DAN PENGEMBANGAN BIODIVERSITAS PADI LOKAL SULAWESI TENGGARA Teguh Wijayanto
Jurnal Agroteknos Vol 3, No 1 (2013)
Publisher : Fakultas Pertanian Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56189/ja.v3i1.2295

Abstract

Biodiversity is high value genetic resources required for the good of human being. Globally, biodiversity has been degraded into a danger level.   Identification of problems, alternative solutions to protect, to conserve, and to utilize the biodiversity is a primary priority. The utilization of local rice cultivars, as a source of germplasm for rice breeding programs must get special attentions to protect the germplasm from vanishing. Biotechnology or genetic engineering technology provides an alternative way and a short cut in manipulating and producing improved plant varieties.  Different kinds of methods can be used to genetically engineer plant species. Since 1980s and ever since, many achievements and improvements have been made by scientists around the world in the area of genetic engineering, including genetic improvement of rice crops.   As an example, scientists have engineered transgenic rice crops that contain pro-vitamin A in the endosperm.  Pro-vitamin A is a substance that does not naturally occur in rice, but is urgently required to prefend human’s eye blindness. Another example, recently a group of scientist has developed super- rice strain that defies triple whammy of stresses (drought, salty-soils, and lack of fertilizer) simultaneously. Keywords: Biodiversity, biotechnology, genetic improvement, and local rice cultivars
EXAMINATION OF CHIMERIC GENETIC STRUCTURE OF T0 TRANSFORMED LUPIN SHOOTS Teguh Wijayanto
Jurnal Agroteknos Vol 1, No 1 (2011)
Publisher : Fakultas Pertanian Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56189/ja.v1i1.2331

Abstract

Kurang  responsifnya eksplan  terhadap  medium  invitro  dan  efisiensi  transformasi yang relatif rendah merupakan kendala umum yang dihadapi pada transformasi genetik tanaman legum.   Pada tanaman lupin, satu spesies legum, sebagian besar klon yang diperbanyak dari satu tunas transgenik (single transformation event) sering tidak mengandung gen yang ditransfer (transgene), dan menghasilkan keturunan yang tidak transgenik, walaupun awalnya plantlet tersebut terbukti transgenik. Kimera (chimerism) diduga sebagai penyebab dari penyimpangan segregasi gen (reduced transgene segregation) ini.  Penelitian ini mencoba untuk membuktikan isu kimera tersebut. Uji in-situ PCR dan uji histokemikal GUS & lokalisasi selular dari transgen (dalam hal ini gen gus) dilakukan untuk mengetahui apakah dan dimana transgen tersebut terekspresi pada tunas lupin trasngenik generasi T0  tersebut.   Uji in-situ PCR dan histokemikal GUS menunjukkan bahwa GUS umumnya terekspresi pada beberapa bagian dalam batang tunas (plantlet T0) dan tidak tersebar merata. Hasil ini memberikan bukti struktural yang penting bahwa transgen terekspresi  secara  sektorial  pada  tunas  lupin  generasi  T0.    Ini  sekaligus  membuktikan bahwa tunas lupin generasi T0  benar-benar kimera, dan menjadi penyebab penyimpangan segregasi transgen pada generasi T1.   Upaya-upaya perlu dilakukan untuk mengurangi kejadian kimera ini, sekaligus mendapatkan ekspresi gen yang solid pada tunas (plantlet) lupin (solid transformant). Key words:   Chimera, transgene segregation, in-situ PCR, GUS histochemical assay
PENGELOMPOKAN PATOTIPE Xanthomonas oryzae pv. oryzae ASAL SULAWESI TENGGARA MENGGUNAKAN PADI GALUR ISOGENIK IRRI H Syair; Samirin Samirin; Teguh Wijayanto; Andi Khaeruni
Jurnal Agroteknos Vol 2, No 1 (2012)
Publisher : Fakultas Pertanian Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56189/ja.v2i1.2260

Abstract

This Research aimed at grouping of Xanthomonas oryzae pv. oryzae pathotypes in rice cropping center of Southeast Sulawesi using isogenic lines of IRRI, as well as to know the Xanthomonas oryzae pv. oryzae pathotypes dominating and spreading in rice cropping center of Southeast Sulawesi. The pathotype grouping was determined by leaf innoculated cutting method using 30 isolates of Xanthomonas oryzae pv. oryzae from Southeast Sulawesi in 10 isogenic lines, namely IRBB1, IRBB2, IRBB3, IRBB4, IRBB5, IRBB7, IRBB10, IRBB11, IRBB13, and IRBB21. Each isolate was inoculated on the 10 isogenic lines and repeated twice. Observation  of disease intensity was conducted randomly on five leaves at four weeks after inoculation. The disease intensity was measured by the ratio of the length (cm) of leaf blight symptom to total the length of leaf sample (cm). The research results indicated there were seven pathotypes of Xanthomonas oryzae pv. oryzae in Southeast Sulawesi, i.e : pathotype X, XI, XII and four new pathotypes. Pathotype XII was the dominant pathotype, abort 79.31% of the total isolates and spreading in three regencies, Kolaka, Konawe, and South Konawe. Keywords: bacterial leaf blight, Xanthomonas oryzae pv. oryzae, pathotype
PERBANYAKAN BIBIT JERUK SECARA IN-VITRO DAN BEBAS PENYAKIT CVPD Teguh Wijayanto; Muhammad Taufik; Dirvamena Boer; Ni Wayan Sri Suliartini
Jurnal Agroteknos Vol 1, No 3 (2011)
Publisher : Fakultas Pertanian Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56189/ja.v1i3.2249

Abstract

Abstract Citrus Vein  Phloem Degeneration (CVPD) is  one  of  the  most important diseases of citrus. Worldwide and national yield losses of citrus production due to this disease infection have been very significant.   This research ultimately aimed at producing citrus stocks through in-vitro culture, and free of CVPD.  Citrus seeds were used as explant and cultured on MS and WPM in-vitro media, supplemented with malt extract. Citrus plantlets were tested for the presence of  CVPD DNA using CVPD specific primers in PCR reactions.   Research results showed that MS basal medium supplemented with malt extract was quite good for in- vitro production of citrus plantlets. Plantlets were negative for CVPD infection based on PCR tests.  CVPD-free seedlings (Citrus reticulata) have been grafted with citrus rootstock (Citrus sinensis).  Grafted citrus seedlings were also proven to be negative for CVPD infection based on similar PCR tests.  Citrus seedlings/stocks produced by in-vitro culture, and free of CVPD, are now available for further growth. Keywords: Citrus Vein Phloem Degeneration (CVPD), in-vitro culture, PCR
PENINGKATAN PENGETAHUAN PETANI MELALUI INOVASI TEKNOLOGI PEMANFAATAN VEGETASI SEKUNDER SEBAGAI BAHAN PUPUK ORGANIK UNTUK MENINGKATKAN KESUBURAN TANAH PADA LAHAN JAGUNG DI DESA WARAMBE KECAMATAN PARIGI KABUPATEN MUNA Nini Mila Rahni; Gusti Ayu Kadek Sutariati; Teguh Wijayanto; Zulfikar Zulfikar; Rachmi Hariaty Hasan
Jurnal Pengabdian NUSANTARA Vol 3, No 2 (2023): Juli - Desember
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jpnus.v3i2.40471

Abstract

The Internal Partnership Program (PKMI) for faculty service is conducted in Warambe Village, Parigi District, Muna Regency, Southeast Sulawesi, with the target audience being the Labotoro Farmers Group. The issues encountered by the farmers' group include poor soil fertility, specifically low-quality land, and a lack of knowledge regarding the production of organic fertilizers based on secondary vegetation. Additionally, there is a significant amount of agricultural waste and secondary vegetation available, but they have not been maximally utilized as raw materials for enhancing soil fertility and as a nutrient source for corn plants due to a lack of knowledge in this area. The proposed solution and targets are to provide technical guidance and demonstrate the production of organic fertilizers for soil fertility enhancement, as well as to transfer this technological innovation to the farmers. The dissemination and adoption process of this technology is expected to increase farmers' income through increased production of yellow corn crops. The results obtained from the provided solution include an 85% increase in the understanding and knowledge of the members of the farmers' group regarding the utilization of secondary vegetation for organic fertilizer and soil fertility enhancement in their corn fields. The technology of utilizing secondary vegetation has not been previously implemented by the partner group, so this activity has contributed to the farmers' knowledge and skills in improving land productivity, especially within the Labotoro Farmers Group
PENGARUH PEMBERIAN BERBAGAI JENIS PUPUK KANDANG TERHADAP PRODUKSI TANAMAN KACANG HIJAU (Vigna radiata L. MUHAMAD NUR ALAMSYAH; LAODE SABARUDDIN; ANDI NURMAS; TEGUH WIJAYANTO; MIRZA ARSIATY ARSYAD; LA ODE SAFUAN
Jurnal Agroteknos Vol 13, No 1 (2023)
Publisher : Fakultas Pertanian Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56189/ja.v13i1.43284

Abstract

This study aims to determine the effect of the application of various types of manure on the production of mung bean. The research was carried out at the Field Laboratory of Experimental Gardens II, Faculty of Agriculture, Halu Oleo University, Kendari, from October to December 2021. The study used a randomized block design consisting of four treatments, namely control (P0), cow manure 10 t ha-1 (P1), goat manure 10 t ha-1 (P2) and chicken manure 10 t ha-1 (P3). Each treatment was repeated 4 times so that there were 16 experimental units. Variables observed were number of pods, pod weight, seed weight, weight of 100 seeds, seed production ton ha-1, analyzed by variance. If the variance shows a significant effect, then proceed with the Least Significant Difference Test (BNT) at the 95% confidence level. The results showed that the application of manure can increase the production of mung bean plants. Treatment of chicken manure 10 t ha-1 (P3) gave a better effect on the number of pods, pod weight, seed weight and seed production t ha-1.
PEMANFAATAN LIMBAH PERTANIAN & TERNAK SEBAGAI BAHAN PUPUK ORGANIK UNTUK USAHA PERTANIAN Teguh Wijayanto; La Ode Arfan Dedu; Anima Hisein
Anoa : Jurnal Pengabdian Masyarakat Sosial, Politik, Budaya, Hukum, Ekonomi Vol 4, No 2 (2023): Oktober
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52423/anoa.v4i2.45921

Abstract

The manufacture and application of compost fertilizer and POP technology (organic fertilizer plus) was carried out in the community in Alebo Village and/or Masagena, Konda District, South Konawe Regency, both for non-productive communities and owners of livestock waste processing. Alebo and Masagena villages are vegetable and annual crop farming areas, which are cultivated throughout the year and generally depend heavily on chemical fertilizers. Therefore, POP and/or compost technology is needed to improve the quality of soil nutrients and increase the production of healthy plants. In the villages of Alebo and Masagena there are abundant reservoirs for livestock waste and rice husks but they have not been processed optimally, but are only sold in the form of rice husks and raw cow husks at relatively cheap prices so that they do not provide added value for the village community, so this community service activity was done to improve environmental conditions which had been polluted due to the use of chemical fertilizers, and can also increase the income of local communities by utilizing existing waste to make organic fertilizer (compost and POP). Organic fertilizer has a higher price than livestock waste and unprocessed rice husks. The solution implemented in this service activity was the application of technology to change the use of chemical fertilizers by using POP technology and compost fertilizer so that farming land is even more productive.
Stabilitas Bakteri Endofit Pseudomonas aeruginosa 2RWB2 Dan Pseudomonas aeruginosa 5BRB3 Dalam Formulasi Tepung Dengan Berbagai Bahan Tambahan Novita Pramahsari Putri; Andi Khaeruni; Marnia Ningsi Umar; La Ode Santiaji; Muhammad Taufik; Vit Neru Satrah; Gusti Ayu Kade Sutariati; Teguh Wijayanto
Agrium Vol 21, No 2 (2024)
Publisher : Faculty of Agriculture, Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/agrium.v21i2.16782

Abstract

Bakteri endofit Pseudomonas aeruginosa 2RWB2 dan P. aeruginosa 5BRB3 terbukti efektif mengendalikan Phytophthora palmivora pada persemaian. Agar aplikasi agens hayati  dapat bertahan lama diperlukan suatu formula yang mampu mempertahankan viabilitas dan daya hambat serta kemudahkan penggunaannya. Penelitian bertujuan untuk mengetahui jenis formulasi tepung dan bahan tambahan yang memberikan pengaruh terbaik terhadap viabilitas dan daya hambat bakteri endofit Pseudomonas aeruginosa 2RWB2 dan P. aeruginosa 5BRB3 terhadap P. palmivora. Penelitian dilaksanakan di Laboratorium Proteksi Tanaman Unit Fitopatologi, Fakultas Pertanian Universitas Halu Oleo Kendari. Penelitian disusun menggunakan Rancangan acak lengkap dengan 6 perlakuan formula tepung dan bahan tambahan yang berbeda. Setiap perlakuan diulang sebanyak 3 kali dan dilaksanakan secara paralel dengan dua jenis bakteri endofit yaitu: P. aeruginosa 2RWB2 dan P. aeruginosa 5BRB3. Data dianalisis dengan sidik ragam dan apabila terdapat pengaruh nyata, maka dilanjutkan dengan Uji Jarak Berganda Duncan. Hasil penelitian menunjukkan bahwa tepung talk dan tepung tapioka dapat berfungsi sebagai bahan dasar dalam formulasi agens hayati bakteri endofit P. aeruginosa 2RWB2 dan P. aeruginosa 5BRB3. Formulasi tepung tapioka P6 dengan penambahan pepton memiliki kemampuan terbaik dalam meningkatkan viabilitas bakteri endofit P. aeruginosa 2RWB2 yaitu sebesar 8,34 x 108 CFU/g pada 8 MSI, formulasi P3 memiliki kemampuan daya hambat terbaik yaitu sebesar 87,78% dengan masa simpan formulasi 10 MSI. Sedangkan tepung tapioka P5 memiliki kemampuan terbaik dalam meningkatkan viabilitas bakteri endofit P. aeruginosa 5BRB3 yaitu sebesar 8,14 x 108 CFU/g pada 8 MSI, formulasi P6 memiliki kemampuan daya hambat terbaik yaitu sebesar 88,89% dengan masa simpan formulasi 12 MSI.
Co-Authors Aminuddin Mane Kandari, Aminuddin Mane Andi Awaluddin Andi Khaeruni Andi Khaeruni Andi Khaeruni ANDI KHAERUNI Andi Khaeruni Andi Khaeruni Andi Khaeruni R, Andi Andi Khaeruni R. ANDI NURMAS Anima Hisein Anwar Anwar Asniah Asniah Awaluddin, Andi AZHAR ANSI Azhar Bafadal Azhar Bafadal Bambang Supriadi Candra Ginting Darmawansyah Darmawansyah Dedu, La Ode Arfan Dirvamena Boer DIRVAMENA BOER Dirvamena Boer Eko Aprianto Johan Erwin Najamuddin Gusnawaty Gusnawaty Gusti Ayu Kade Sutariati Gusti Ayu Kadek Sutariati Gusti R. Sadimantara Gusti Ray Sadimantara H Syair Hadi Sudarmo Hariyani La Ente La Ode Safuan La Ode Safuan La Ode Santiaji Lailatul Nuraini Laode Sabaruddin Made Etikawati Made Widana Arsana Mangalla, Lukas K. MARIADI MARIADI Marnia Ningsi Umar Mirza Arsiaty Arsyad MUHAMAD NUR ALAMSYAH Muhammad Taufik Muhammad Taufik Muhammad Taufik Muhammad Taufik Musa, Musril Hakim Nasution, Annio Indah Lestari Neli Susanti Ni Wayan Sri Suliartini Ni Wayan Sri Suliartini Nini Mila Rahni NORMA ARIF, NORMA Novita Pramahsari Putri Nur Aida, Nur Nuraida, Waode Rachmi Hariaty Hasan Samirin Samirin Satrah, Vit Neru Sitti Aida A. Taridala Surni Surni Syair Syair Tanza Nirmala Vit Neru Satrah Vit Neru Satrah Vit Neru Satrah WA ODE AFU Wa Ode Rahzia Yani Waode Siti Anima Hisein Waode Siti Anima Hisein Zohorman Zohorman Zohorman Zohorman Zulfikar Zulfikar