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All Journal Buletin Veteriner Udayana Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Advances in Tropical Biodiversity and Environmental Sciences Jurnal Kedokteran Hewan Journal of Veterinary and Animal Sciences
I Wayan Wirata
Fakultas Kedokteran Hewan Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Kajian Toksisitas Sub Akut Ekstrak Etanol Anggur Bali pada Tikus Wistar Luh Made Sudimartini; I Wayan Nico Fajar Gunawan; I Wayan Wirata; I Made Kardena; Anak Agung Gde Oka Darmayudha; Amelia Avianti Saritjang
Buletin Veteriner Udayana Vol. 13 No. 1 Pebruari 2021
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.498 KB) | DOI: 10.24843/bulvet.2021.v13.i01.p02

Abstract

Bali grapes are a source of antioxidants with high flavonoids and anthocyanins, which have many health benefits such as lowering cholesterol and anti-cancer, inhibiting aging, and protecting the heart. Many people consume Balinese grapes by eating directly, drink in the form of juice or extract form because of its benefits. The purpose of this study was to determine the sub-acute toxicity of the ethanol extract of bali grapes (Vitis vinera cv. Alphonso lavelle) to changes in SGOT and SGPT of male Wistar rat liver. This test includes a sub-acute toxicity test and a pharmacological effect test. This research is an experimental study with a completely randomized design using a post-test-only control group design. The number of white rats used in this study were 24 rats, which were divided into four groups of control groups, groups of mice given ethanol extract of bali grapes 0.3 g / ml/kg BW (P1), groups of mice given ethanol extract of grapes bali 3 g / ml/kg BW (P2), and the group of rats that were given 30 g / ml kg BW (P3) of ethanol extract of bali grapes. Each group consisted of 6 white male rats. In this study, the grape ethanol extract was presented orally once per day for 28 days. After 28 days of treatment, Wistar rats were euthanized, and blood samples were taken through Chantus Medial Orbitalis and then put into non-EDTA tubes. The blood sample that has been centrifuged and becomes serum is then inserted into the Eppendorf tube, which is used to test the levels of SGOT and SGPT. The results of the One-Way ANOVA analysis on SGPT levels in the liver of rats showed a significant difference (P <0.05), so that further analysis was necessary using the Duncan-test. Based on the results of the analysis using the Duncan Test, changes occurred in the P3 treatment group of rats given the ethanol extract of Bali grapes at a dose of 30 g / ml/kg BW, which was significantly different from the control treatment group (P0) so that the mixture of these materials was included in the class of materials that were practically non-toxic.
Prevalensi Toxocara vitulorum Pada Induk Dan Anak Sapi Bali Di Wilayah Bali Timur Kadek Karang Agustina; Anak Agung Gde Oka Dharmayudha; I Wayan Wirata
Buletin Veteriner Udayana Vol. 5 No.1 Pebruari 2013
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Penelitian ini bertujuan untuk mengetahui prevalensi infeksi cacing Toxocara vitulorum pada induk dan anak sapi bali di wilayah Bali Timur. Sebanyak 720 sampel feses sapi bali yang terdiri dari 360 sampel feses induk sapi bali dan 360 sampel feses anakan (pedet) sapi bali telah diperiksa menggunakan metode pengapungan. Hasil pemeriksaan secara keseluruhan menunjukkan prevalensi T. vitulorum pada sapi bali di wilayah Bali timur sebesar 39,4 %. Dari 360 induk sapi bali terdapat 153 (42,5%) sampel yang positif terinfeksi T. vitulorum. Sedangkan dari pemeriksaan 360 sampel feses pedet sapi bali, terdeteksi sebanyak 131 (36,4%) sampel pedet yang terinfeksi T.vitulorum.
PELAYANAN KESEHATAN HEWAN PADA SAPI BALI DI DESA SUSUT, KECAMATAN SUSUT, KABUPATEN BANGLI. Luh Made Sudimantini; I Wayan Nico Fajar Gunawan; i Wayan Wirata; Luh Gde Sri Surya Heryani
Buletin Udayana Mengabdi Vol 19 No 2 (2020): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Pengabdian ini bertujuan untuk memberikan informasi tentang kesehatan hewan ternak, manajemen pemeliharaan ternak serta penanggulangan penyakit pada hewan ternak sehingga dapat meningkatkan dan menekan angka kerugian ekonomi peternak sapi Bali dan dapat meningkatkan swasembada daging sapi di Desa Susut, Kecamatan Susut, Kabupaten Bangli. Kegiatan pengabdian ini dilakukan dalam bentuk pelayanan kesehatan dengan pengumpulan ternak sapi pada suatu tempat berupa pemberian vitamin, obat cacing dan spraying terhadap ternak yang sehat dan melakukan pengobatan terhadap ternak yang sakit, serta diskusi dengan peternak tentang arti penting memelihara kesehatan ternak. Sasaran kegiatan pengabdian berupa diskusi dengan peternak sapi dan pelayanan kesehatan ternak sapi bali di Simantri 268, Gapoktan Merta Shanti Desa Susut, Kecamatan Susut, Kabupaten Bangli. Jumlah hewan sapi yang dilayani pada pelaksanaan ini sebanyak 24 ekor sapi. Pelayanan kesehatan hewan ternak berupa pemberian vitamin (24 ekor), obat cacing (20 ekor), spraying butox (24 ekor) serta pemberian injeksi ivomec (2 ekor) bagi hewan yang mengalami gatal-gatal pada kulit.
Pola Distribusi Unggas dari Pasar Tradisonal Berperan dalam Penyebaran Virus Flu Burung I MADE SUMA ANTARA; I NYOMAN SUARTHA; I KADEK SAKA WIRYANA; I MADE SUKADA; I WAYAN WIRATA; I GUSTI NGURAH DIBYA PRASETYA; NI MADE RITHA KRISNA DEWI; TRI KOMALA SARI; I GUSTI NGURAH KADE MAHARDIKA
Jurnal Veteriner Vol 10 No 2 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study has been carried out to map the distribution pattern of poultry from traditional market toreduce the transmission risk of avian influenza virus. The data were collected from threes markets wherepoultry are sold, namely in Bringkit of Badung Regency, Kumbasari of Denpasar City, and Kediri ofTabanan Regency. Data collections was based on interviews using questionnaire. Poultry from all marketsare distributed throughout Bali. Poultry are traded mainly for religious ceremony and immediatelyslaughtered as it arrives at the consumer’s house. The distribution pattern of poultry seems to play asignificant role in the disseminations of avian influenza virus. The right implementation of biosecurity intraditional markets is highly recommended to curb the risk.
Laporan Kasus: Obstruksi Rektum dan Uretra Akibat Fibroma Serviks pada Anjing Golden Retriever (RECTAL AND URETRA OBSTRUCTION DUE TO FIBROMA CERVICES IN GOLDEN RETRIEVER DOG: CASE REPORT ) I Nyoman Suartha; I Wayan Wirata; I Wayan Gorda; Agatha Serena Lumban Tobing; I Ketut Eli Supartika
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Rectal obstruction in dogs is generally caused by foreign objects such as bone or hard feces, whereas urethral obstruction is generally caused by calculi. Cases of rectal and urethral obstruction due to tumors or neoplasia in the female reproductive tract of dogs are rare. Fibroma is one type of benign tumor that attacks fibroblast tissue. The formation of the tumor results in obstruction of the gastrointestinal tract and urine. This paper aims to raise cases of rectum obstruction and vesica urinaria in dogs. Fourth yearold female golden retriever dogs was admitted to Veterinary Hospital Faculty of Veterinary Medicine Udayana University. According to the case history of owner, the dog was suffered weakness, anoreksia, bally and tend. The dog was difficult to urinate and poo. The stool was suppressed like “ornament cake birth day”. All of health condition were normal. Clinical examination revealed presence mass like tumor attached to the cervix with consistence hard and irregular form. Ultrasonography examination was distended bladder without calculi form. Hematological routine have leucophilia, anemia and thrombositopenia. After general anesthesia with ketamine HCl and xylazine, the mass with irregular form and hard consistence was surgical removed. Histophatological section were revealed presence of fibroma which represented by arrangement of cell that have feature tapering cell as fibroblast without mitosis. The mass was diagnosed as fibroma. It concluded that golden retriever dogs had obstruction of rectum and vesica urinaria as result of cervical fibroma tumors.
Peranan Pedagang Unggas dalam Penyebaran Virus Avian Influenza I Nyoman Suartha; I Made Suma Antara; I Kadek Saka Wiryana; I Made Sukada; I Wayan Wirata; Ni Made Ritha Krisna Dewi; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A questionnaire surveillence have been carried out in three different traditional markets (ie. Beringkitin badung district, Kumbasari in Denpasar, Kediri in Tabanan district) in order to understand the role ofpaultry traders behavior in transmitting of avian influenza virus. Of 150 quationares collected most oftraders (66.7%) kept the animals for 1-3 days before it was marketed. Traders bin Beringkit and Kediri(76.3%) used to mix different species of birds in their cages, whereas none of the traders from Kumbasaridoing that. When hygienec and sanitation aspects were considered (ie. Washing and desinfectan sprayingfor cages) it was found that the behavior of traders varied markedly between the 3 different market. Inconclusion the traders awareness to especially bird flue infection and implementation of biosecurity isvery low.
Deteksi Virus Classical Swine Fever di Bali dengan RT-PCR I Wayan Wirata; Ida Ayu Sri Chandra Dewi; I Gusti Ngurah Narendra Putra1,; Ida Bagus Oka Winaya; Ida Bagus Kade Suardana3,; Tri Komala Sari; I Nyoman Suartha; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Classical Swine Fever (CSF) virus has been confirmed for the first time in pig in Bali. The object of thisstudy was suspected CSF cases diagnosed at the diagnostic laboratory assistantship of the Faculty ofVeterinary Medicine, Udayana University, in 2007-2008. Total number of cases was 12. Case recordsincluded the signalment of case (breed, age, body weight, and the origin of respective case), clinical signs,post-mortem lesions, and histological pictures. CSF virus was confirmed using the standardized reversetranscriptase-polymerase chain reaction (RT-PCR) for CSF from European Union. One RT-PCR productwas sequenced. CSF virus was confirmed in seven out of 12 cases (58%). The cDNA sequence wasconfirmed to be specific of CSF E2 protein coding region with 98% homology to one isolate from China thatwas available in GeneBank. Further works are recommended to elucidate the sensitivity of RT-PCR, toclarify some differential diagnose, and to find out the genetic variation of CSF virus in Bali.Key words: classical swine fever virus, Bali, RT-PCR
Vaksin Polivalen Untuk Mencegah Penyakit Flu Burung (POLIVALEN VACCINE TO PREVENT BIRD FLU DISEASES) I Nyoman Suartha; I Wayan Wirata; I Gusti Ngurah Narendra Putra; Ni Made Ritha Krisna Dewi; I Made Suma Anthara; I Wayan Teguh Wibawan; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was carried out to determine the use of bird flu polyvalent vaccines containing two or threeor more virus isolates representating of circulating viruses in the region. Three seed isolates of avianinfluenza H5N1 virus were used in this experiment. The isolates were Chicken/Denpasar/Unud-01/2004,Chicken/Klungkung/Unud-12/2006, and Chicken/Jembrana/Unud-17/2006. The seeds were inactivatedusing 0.01% formaldehide than mixed (AI3G) alumunium hidroxide adjuvant and then injectedintramuscularly to Isa Brown layer chicken at 3 weeks of age and repeated at the age of 5 weeks. The doseof each seed virus was 27 HA units. Sera were collected at one and two weeks after the second vaccination.The result showed that the arithmetic meant titer (AMT) of sera that tested with homologous isolate washigher than the test using a heterologous isolates, in the standard haemaglutination inhibition (HI) assay.The mixed AI3G vaccine produced a uniform AMT against the constituent isolates, while vaccines withindividual isolate yielded a lower and more variation in AMT. Further experiments using a commercialhomologous H5N1 and heterologous H5N2 commercial vaccines has resulted AMT that 1-4 log lower thanAI3G vaccine. It is concluded that polyvalent vaccine with field seed isolates is recommended to be appliedin the poultry farm in Indonesia.
Survei Penyakit Porcine Reproductive and Respiratory Syndrome pada Peternakan Babi di Bali (SEROLOGICAL SURVEILLANCE OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME IN PIGGERIES IN BALI) I Nyoman Suartha; I Made Suma Anthara; I Wayan Wirata; Tri Komala Sari; Ni Made Ritha Krisna Dewi; I Gusti Ngurah Narendra; I Gusti Ngurah Mahardika
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study aimed to determine the presence and burden of Porcine Respiration and ReproductiveSyndrome (PRRS) virus in pig farms in Bali. A total of 305 sera samples were collected from 10 intensivepig farms and backyard piggeries located in eight districts, in Bali. The PRRS antibody and the virus wasdetected using enzyme linked immunosorbent assay (ELISA) and transverse reverse polymerase chainreaction (RT-PCR), respectively. The results showed that, generally the average percentage of positiveswine anti-PRRS antibody was 13.4%, 14.3%,  and 11.7% in the backyard farms and commercial farms,respectively. Whereas, the detection rate of PRRS virus was 8.9% (15.3% and 5.6% in the backyard farmand commercial farms, respectively). It was concluded that PRRS virus is endemic in pigs, in Bali.Vaccination, management, biosafety, and quarantine  should be implemented to prevent the economicloss due to PRRS.
Radiographic Evaluation of Rabbit Femur Implanted Bali Cattle Bone Graft I Wayan Wirata; Steven Dwi Purbantoro; Luh Made Sudimartini; IWN Fajar Gunawan; AA Oka Dharmayudha; IGAG Putra Pemayun
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bone xenograft from cattle bone is commonly used to treat a comminuted fracture case. This study aims to know the process of fractured-femur bone healing in rabbit post-implantation powder bone graft from cortical femur bone of Bali cattle evaluated by radiographs. Ten male local rabbits were used in this study, which were divided into 2 groups randomly. Group I (KI) as control, the diaphysis of femur bone was drilled with a diameter of 5 mm without implanting the bone graft, while Group II (KII), the diaphysis of femur bone was drilled 2 holes with a diameter of 5 mm each and with distance 20 mm, substituted with mineralized powder bone graft for the proximal hole (KIIa) and demineralized powder bone graft for the distal hole (KIIb). Fracture healing evaluation was done at week 0 (24 hours), 2, 4, and 6 postoperative by monitoring the growth of callus, fracture line, and union process with radiograph based classification according to Hammer et al., tabulated statistically, and presented descriptively. The results showed that KI and KII were in the sequel of fracture healing but had not reached remodeling phase perfectly. In conclusion, mineralized and demineralized powder bone graft used in this study was as osteoconductive and the use of bone graft shows no different significance and time shows different significance to fracture healing.
Co-Authors Abd. Rasyid Syamsuri Adigunawan, I Wayan Widya Agatha Serena Lumban Tobing Amelia Avianti Saritjang Anak Agung Gde Jaya Wardhita, Anak Agung Gde Jaya Anak Agung Gde Oka Darmayudha Anak Agung Gde Oka Dharmayudha Anak Agung Jaya Wardhita Anastasia Bhala Andini, Ni Made Ria Antari, Gusti Ayu Made Sri Ayu Ratnasari Deo Lauda Putra Dewi, Kadek Evi Dian Puspita Gunawan, I Wayan Nico Fajar Gunawan, Stefanus Andre I Gusti Agung Gede Putra Pemayun I GUSTI NGURAH DIBYA PRASETYA I Gusti Ngurah Kade Mahardika I Gusti Ngurah Mahardika I Gusti Ngurah Narendra I Gusti Ngurah Narendra Putra I Gusti Ngurah Narendra Putra1, I Gusti Ngurah Sudisma I KADEK SAKA WIRYANA I Ketut Anom Dada I Ketut Berata I Ketut Eli Supartika I Made Kardena I Made Merdana I Made Sukada I MADE SUMA ANTARA I Made Suma Anthara i Nengah Wandia I Nyoman Suartha I Wayan Gorda I Wayan Juli Sumadi I wayan Teguh Wibawan Ida Ayu Sri Chandra Dewi Ida Bagus Kade Suardana Ida Bagus Oka Winaya Iwan Harjono Utama Kadek Karang Agustina Komang Darma Yudha Putra Luh Gde Sri Surya Heryani Luh Made Sudimantini Luh Made Sudimantini Luh Made Sudimartini Made Suma Anthara Maria Clafita Witoko Mildawati Mildawati Ni Kadek Eka Widiadnyani Ni Luh Eka Setiasih Ni Made Rita Krisna Dewi Ni Made Ritha Krisna Dewi Ni Putu Trisna Asih Purbantoro, Steven Dwi Putra, Komang Darma Yudha Putu Henrywaesa Sudipa Rohmandhani, Roby Sasmita, Debbie Aprillia Yona Steven Dwi Purbantoro Steven Dwi Purbantoro Surbakti, Yeyen Fami Gressia Br Tahalli, Tahalli Tessa Saputri Marmanto TRI KOMALA SARI Warditha, Anak Agung Gde Jaya Wayan Herry Gumawan Wulandari Wulandari