Articles
<![CDATA[AN EFFECTIVE METHOD FOR DNA EXTRACTION OF MATURE LEAF OF SAPODILLA (Manilkara zapota (L.) van Royen) ]]>
<![CDATA[Sari, Vega Kartika]]>;
<![CDATA[Murti, Rudi Hari]]>
<![CDATA[ AGRIVITA, Journal of Agricultural Science Vol 37, No 1 (2015) ]]>
Publisher : <![CDATA[Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation ]]>
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<![CDATA[Perennial crop leaves contain polysaccharides, polyphenols, and other secondary metabolites in high concentration. The presence of those compounds inhibit enzymatic activities and amplification of DNA. The existing extraction methods were not able to dissociate the metabolites contaminants of sapodilla (Manilkara zapota (L.) van Royen) leaves and thus resulting in low quality of extracted DNA. The aim of this experiment was to develop an effective method to extract DNA from mature leaf samples of sapodilla (Manilkara zapota (L.) van Royen). Fifth modification of Doyle & Doyle DNA extraction protocol with modified concentration of buffer reagent (consisted of: CTAB 2.8%; NaCl 2.5M; mercaptoethanol 3%, and PVP 2.5%) and repetition of some phase purification (liquid nitrogen; three times CIAA; two times ethanol 70%, RNAse 1µl) generated high quality DNA and clear band of PCR amplification using RAPD primers.   Keywords: buffer modifications, DNA extraction, sapodilla]]>
<![CDATA[KOMBINASI PUPUK KANDANG SAPI, ASAM HUMAT DAN MIKORIZA TERHADAP INFEKSI AKAR BERMIKORIZA TANAMAN CABAI DAN KETERSEDIAAN UNSUR HARA TANAH UDIPSAMMENTS ]]>
<![CDATA[Sukri, M. Zayin]]>;
<![CDATA[Firgiyanto, Refa]]>;
<![CDATA[Sari, Vega Kartika]]>;
<![CDATA[Basuki, Basuki]]>
<![CDATA[ Jurnal Penelitian Pertanian Terapan Vol 19, No 2 (2019) ]]>
Publisher : <![CDATA[Politeknik Negeri Lampung. ]]>
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DOI: 10.25181/jppt.v19i2.1450
<![CDATA[This research is an experimental study that aims to examine the combination of cattle manure, humic acid, and mycorrhizae to increase mycorrhizal infections in the roots of chili plants in the Udipsamments soil in Puger District, Jember Regency, which was carried out in May-November 2018. The research design used was a factorial complete randomized block design with 3 factors that were repeated 3 times. The first factor is cattle manure (0 tons / ha, 10 tons / ha, 20 tons / ha), the second factor is humic acid (0 ppm / ha, 200 ppm / ha), and the third factor is mycorrhiza (0 g / plant , 30 g / plant). The results showed that the combination of cattle manure, humic acid, and mycorrhiza inoculants to chili plant roots was able to increase the root mycorrhizal infection of chili plants and increase the nutrients available in the soil by 191.84% for nitrogen and 392.93% for phosphorus.]]>
<![CDATA[Efektifitas Dolomit Dalam Mempertahankan pH Tanah Inceptisol Perkebunan Tebu Blimbing Djatiroto ]]>
<![CDATA[Basuki, Basuki]]>;
<![CDATA[Sari, Vega Kartika]]>
<![CDATA[ Buletin Tanaman Tembakau, Serat & Minyak Industri Vol 11, No 2 (2019): OKTOBER 2019 ]]>
Publisher : <![CDATA[Balai Penelitian Tanaman Pemanis dan Serat ]]>
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DOI: 10.21082/btsm.v11n2.2019.58-64
<![CDATA[Produktivitas tebu dipengaruhi oleh lingkungan biotik dan abiotik. Lingkungan abiotik salah satunya adalah pH tanah yang dipengaruhi oleh bahan induk tanah, dan pupuk. Penggunaan pupuk anorganik secara terusmenerus menurunkan pH tanah. Penggunaan amelioran seperti dolomit dan kapur pertanian (kaptan) dapat menjadi solusi untuk meningkatkan pH tanah. Penelitian ini bertujuan untuk mengetahui efektivitas dolomit terhadap pH tanah inceptisol di perkebunan tebu. Penelitian menggunakan metode observasi dan eksperimen. Eksperimen dengan perlakuan: A. 160 kg N/ha + 72 kg P2O5 /ha + 60 kg K2O/ha; B. 160 kg N/ha + 72 kg P2O5 /ha + 60 kg K2O/ha+ kaptan 2.000 kg/ha; dan C. 160 kg N/ha +72 kg P2O5 /ha + 60 kg K2O/ha+ dolomit 2.000 kg/ha. Hasil penelitian menunjukkan bahwa pengunaan dolomit dengan dosis 2.000 kg/ha lebih efektif mempertahankan pH tanah dibandingkan kaptan. Dolomit mempertahankan pH tanah hingga 17 bulan setelah aplikasi. Nilai pH 17 bulan setelah aplikasi dolomite adalah 6,64; sedangkan pada perlakuan kaptan memiliki pH tanah 5,56. Reaksi dolomit di tanah dalam mempertahankan pH tanah adalah 1, 26 kali lebih efektif dibandingkan kaptanThe Effectiveness of Dolomite in Maintaining Inceptisol Soil pH of Blimbing Sugarcane Plantation in DjatirotoSugarcane productivity is influenced by the biotic and abiotic environment. One of the abiotic environments is soil pH. Soil pH is influenced by soil parent material, and fertilizer. The use of inorganic fertilizers continuously reduces soil pH. The use of ameliorants such as the use of dolomite and agricultural lime can be a solution. This study aims to determine the effectiveness of dolomite on the soil pH of inceptisol soil Sugar Cane Plantation. The research method uses observation and experimental methods. Experiments with treatment included A. 160 kg nitrogen / hectare + 72 kg P2O5 / hectare + 60 kg K2O / hectare; B. 160 kg nitrogen / hectare + 72 kg P2O5/ hectare + 60 kg K2O / hectare + 2,000 kg agricultural lime / hectare; C. 160 kg nitrogen / hectare + 72 kg P2O5 / hectare + 60 kg K2O / hectare + dolomite 20 quintal / hectare. The results showed the use of dolomite at a dose of 2,000 kg / hectare was more effective in maintaining soil pH compared to agricultural lime. Dolomite maintains soil pH for up to 17 months after application. The pH value, the dolomite treatment was 6.64, while the agricultural lime soil pH treatment was 5.56. Dolomite reaction in the soil in maintaining soil pH of 1.26 times agricultural lime.]]>
<![CDATA[Keragaan Varietas Tebu Unggul Baru pada Fase Pembibitan dengan Pemberian Nano Silika ]]>
<![CDATA[Sari, Vega Kartika]]>;
<![CDATA[Hariyono, Kacung]]>
<![CDATA[ AGRITROP Vol 18, No 2 (2020): Agritrop: Jurnal Ilmu-Ilmu Pertanian ]]>
Publisher : <![CDATA[Universitas Muhammadiyah Jember ]]>
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DOI: 10.32528/agritrop.v18i2.3795
<![CDATA[Tebu merupakan akumulator silika yang berarti memerlukan banyak asupan silika. Pupuk silika (Si) dapat berasal dari berbagai sumber dan terobosan teknologi saat ini tersedianya Si dalam bentuk nano. Beberapa varietas tebu unggul baru belum diuji pada lahan marginal. Tujuan penelitian ini untuk mengetahui pengaruh pemberian nano silika terhadap pertumbuhan fase bibit beberapa varietas unggul baru pada tanah entisol. Rancangan penelitian ini menggunakan Rancangan Acak Kelompok Faktorial dengan 2 faktor. Faktor pertama aplikasi nano Si (S) terdiri atas aplikasi nano Si 0% (S1) dan aplikasi nano Si 30% (S2). Faktor kedua varietas unggul baru terdiri atas Bululawang (V1), NX-01 (V2), NX-02 (V3), dan VMC 86-550 (V4). Hasil penelitian menunjukkan pemberian nano Si tidak berpengaruh nyata pada pertumbuhan tebu fase pembibitan, sedangkan varietas dan interaksi nano Si x V berpengaruh nyata terhadap pertumbuhan tebu. Varietas VMC 86-550 menunjukkan rata-rata pertumbuhan yang lebih baik pada seluruh variabel pengamatan.]]>
<![CDATA[Pemanfaatan Bahan Organik Sebagai Solusi Solum Tanah Dangkal di Desa Slateng Kecamatan Ledokombo Kaki Gunung Raung ]]>
<![CDATA[Basuki Basuki]]>;
<![CDATA[Vega Kartika Sari]]>;
<![CDATA[Marga Mandala]]>
<![CDATA[ Jurnal Pengabdian Magister Pendidikan IPA Vol 5 No 1 (2022): Januari - Maret ]]>
Publisher : <![CDATA[Universitas Mataram ]]>
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DOI: 10.29303/jpmpi.v5i1.1407
<![CDATA[Slateng village is located at the western foot of Mount Raung in Ledokombo District, Jember Regency. Farming and cattle ranching provide the majority of the people's income. Agricultural land productivity is still low compared the production standard based on plant variety descriptions. The cultivated land has shallow solum. This activity aims to provide training for Slateng’s farmer to boost cultivated plant yield by enhancing the properties of shallow solum soil and producing organic fertilizer from agricultural waste. Socialization and practice are the methods employed for this activity. The increased of participants' knowledge grew from 30% to 100% as a result of the exercise. Participants were quite interested with the training on creating organic fertilizers, which required active participation from participants, particularly farmers, with a satisfaction rate of 90%. This community service activities can help increase the production of cultivated plants, particularly on marginal land with shallow solum soil.]]>
<![CDATA[Efektifitas Dolomit Dalam Mempertahankan pH Tanah Inceptisol Perkebunan Tebu Blimbing Djatiroto ]]>
<![CDATA[Basuki Basuki]]>;
<![CDATA[Vega Kartika Sari]]>
<![CDATA[ Buletin Tanaman Tembakau, Serat & Minyak Industri Vol 11, No 2 (2019): OKTOBER 2019 ]]>
Publisher : <![CDATA[Balai Penelitian Tanaman Pemanis dan Serat ]]>
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DOI: 10.21082/btsm.v11n2.2019.58-64
<![CDATA[Produktivitas tebu dipengaruhi oleh lingkungan biotik dan abiotik. Lingkungan abiotik salah satunya adalah pH tanah yang dipengaruhi oleh bahan induk tanah, dan pupuk. Penggunaan pupuk anorganik secara terusmenerus menurunkan pH tanah. Penggunaan amelioran seperti dolomit dan kapur pertanian (kaptan) dapat menjadi solusi untuk meningkatkan pH tanah. Penelitian ini bertujuan untuk mengetahui efektivitas dolomit terhadap pH tanah inceptisol di perkebunan tebu. Penelitian menggunakan metode observasi dan eksperimen. Eksperimen dengan perlakuan: A. 160 kg N/ha + 72 kg P2O5 /ha + 60 kg K2O/ha; B. 160 kg N/ha + 72 kg P2O5 /ha + 60 kg K2O/ha+ kaptan 2.000 kg/ha; dan C. 160 kg N/ha +72 kg P2O5 /ha + 60 kg K2O/ha+ dolomit 2.000 kg/ha. Hasil penelitian menunjukkan bahwa pengunaan dolomit dengan dosis 2.000 kg/ha lebih efektif mempertahankan pH tanah dibandingkan kaptan. Dolomit mempertahankan pH tanah hingga 17 bulan setelah aplikasi. Nilai pH 17 bulan setelah aplikasi dolomite adalah 6,64; sedangkan pada perlakuan kaptan memiliki pH tanah 5,56. Reaksi dolomit di tanah dalam mempertahankan pH tanah adalah 1, 26 kali lebih efektif dibandingkan kaptanThe Effectiveness of Dolomite in Maintaining Inceptisol Soil pH of Blimbing Sugarcane Plantation in DjatirotoSugarcane productivity is influenced by the biotic and abiotic environment. One of the abiotic environments is soil pH. Soil pH is influenced by soil parent material, and fertilizer. The use of inorganic fertilizers continuously reduces soil pH. The use of ameliorants such as the use of dolomite and agricultural lime can be a solution. This study aims to determine the effectiveness of dolomite on the soil pH of inceptisol soil Sugar Cane Plantation. The research method uses observation and experimental methods. Experiments with treatment included A. 160 kg nitrogen / hectare + 72 kg P2O5 / hectare + 60 kg K2O / hectare; B. 160 kg nitrogen / hectare + 72 kg P2O5/ hectare + 60 kg K2O / hectare + 2,000 kg agricultural lime / hectare; C. 160 kg nitrogen / hectare + 72 kg P2O5 / hectare + 60 kg K2O / hectare + dolomite 20 quintal / hectare. The results showed the use of dolomite at a dose of 2,000 kg / hectare was more effective in maintaining soil pH compared to agricultural lime. Dolomite maintains soil pH for up to 17 months after application. The pH value, the dolomite treatment was 6.64, while the agricultural lime soil pH treatment was 5.56. Dolomite reaction in the soil in maintaining soil pH of 1.26 times agricultural lime.]]>
<![CDATA[ANALISIS KLASTER DURIAN (Durio zibethinus Murr.) UNGGUL LOKAL DI KABUPATEN JEMBER DAN BANYUWANGI ]]>
<![CDATA[Vega Kartika Sari]]>;
<![CDATA[Eva Rosdiana]]>;
<![CDATA[Sepdian Luri Asmono]]>
<![CDATA[ Jurnal Agroteknologi Vol 11, No 2 (2021): Februari 2021 ]]>
Publisher : <![CDATA[Universitas Islam Negeri Sultan Syarif Kasim Riau ]]>
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DOI: 10.24014/ja.v11i2.9056
<![CDATA[Indonesia is one of the centers of durian diversity in the world and one of the regions that makes durian a superior local fruit crop is Jember and Banyuwangi. Clustering is useful to facilitate the search for certain features of the type of durian. This study aims to determine the durian grouping in two districts namely Jember and Banyuwangi. The materials used were local superior durian fruit and characterization questionnaire. Research using descriptive methods. Characterization data will be analyzed using NTSYS software.The results showed that local superior durian fruit were grouped into 2 groups based on morphological characters. Cluster 1 consists of 4 durians namely Klemben, Hijau, Bajul, and Lambau. Cluster 2 only consists of durian Musang King. Based on the chemical content of durian fruit, durian Lambau from Jember has the highest water content of 75.52%, Musang King from Banyuwangi has a fat content (2.29%), Bajul from Jember has the highest fiber content (11.89%), and Hijau from Banyuwangi has the highest protein (3.14%) and sugar (26.08%) content. Based on PCA showed that Musang King and Hijau have similirity on some morphological characters and chemical fruit contents. The results of correlation analysis of morphological characters and chemical content of durian fruit showed that leaf length was positively correlated with leaf width and protein content. Leaf width is positively correlated with sugar content. Fruit weight is positively correlated with water content.]]>
<![CDATA[Pelatihan Enkapsulasi Pupuk Rhizobium spp pada Media Cair dan Granular untuk Tanaman Kedelai di Desa Sukorejo Kecamatan Bangsalsari Kabupaten Jember ]]>
<![CDATA[Vega Kartika Sari]]>;
<![CDATA[Damanhuri Damanhuri]]>;
<![CDATA[Iqbal Erdiansyah]]>;
<![CDATA[Eliyatiningsih Eliyatiningsih]]>;
<![CDATA[Aditya Wahyu Pratama]]>
<![CDATA[ Journal of Innovation and Applied Technology Vol 6, No 2 (2020) ]]>
Publisher : <![CDATA[Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Brawijaya ]]>
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DOI: 10.21776/ub.jiat.2020.006.02.3
<![CDATA[Desa Sukorejo di Kecamatan Bangsalsari merupakan salah satu sentra kedelai di Kabupaten Jember namun 3 tahun terakhir mengalami penurunan produksi yang disebabkan biaya produksi tidak sebanding dengan pendapatan yang diperoleh. Salah satu upaya untuk menekan biaya produksi adalah dengan menekan penggunaan pupuk kimia. Pupuk N atau pupuk urea pada pertanaman kedelai dapat diganti dengan penggunaan pupuk hayati yang dibuat dari bakteri Rhizobium spp. yang memiliki kemampuan memfiksasi Nitrogen dari udara. Program pengabdian masyarakat ini bertujuan memberikan penyuluhan dan pelatihan pembuatan pupuk N dari Rhizobium menjadi bentuk granular dan cair. Pupuk dalam bentuk granul efisien dalam aplikasinya karena tidak mudah tercuci. Pupuk dalam bentuk cair memiliki keuntungan dapat langsung diserap di perakaran dan dapat membantu merevitalisasi daya olah tanah dan mengemburkan media tanah dengan optimal. Berdasarkan hasil panen demplot yang dilakukan menunjukkan bahwa tanaman kedelai yang diberi perlakuan Rhizobium memberikan hasil yang lebih tinggi dibandingkan tanaman kedelai kontrol.]]>
<![CDATA[Kombinasi Pupuk Kandang Sapi, Asam Humat Dan Mikoriza Terhadap Infeksi Akar Bermikoriza Tanaman Cabai Dan Ketersediaan Unsur Hara Tanah Udipsamments ]]>
<![CDATA[M. Zayin Sukri]]>;
<![CDATA[Refa Firgiyanto]]>;
<![CDATA[Vega Kartika Sari]]>;
<![CDATA[Basuki Basuki]]>
<![CDATA[ Jurnal Penelitian Pertanian Terapan Vol 19 No 2 (2019) ]]>
Publisher : <![CDATA[Politeknik Negeri Lampung. ]]>
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DOI: 10.25181/jppt.v19i2.1450
<![CDATA[This research is an experimental study that aims to examine the combination of cattle manure, humic acid, and mycorrhizae to increase mycorrhizal infections in the roots of chili plants in the Udipsamments soil in Puger District, Jember Regency, which was carried out in May-November 2018. The research design used was a factorial complete randomized block design with 3 factors that were repeated 3 times. The first factor is cattle manure (0 tons / ha, 10 tons / ha, 20 tons / ha), the second factor is humic acid (0 ppm / ha, 200 ppm / ha), and the third factor is mycorrhiza (0 g / plant , 30 g / plant). The results showed that the combination of cattle manure, humic acid, and mycorrhiza inoculants to chili plant roots was able to increase the root mycorrhizal infection of chili plants and increase the nutrients available in the soil by 191.84% for nitrogen and 392.93% for phosphorus.]]>
<![CDATA[Pemanfaatan Vinasse sebagai Pupuk Organik Cair untuk Meningkatkan Pertumbuhan dan Hasil Bunga Kol (Brassica oleracea var. Botrytis L.) ]]>
<![CDATA[Siti Ma'rufah]]>;
<![CDATA[Riza Yuli Rusdiana]]>;
<![CDATA[Vega kartika sari]]>
<![CDATA[ Jurnal Penelitian Pertanian Terapan Vol 20 No 1 (2020) ]]>
Publisher : <![CDATA[Politeknik Negeri Lampung. ]]>
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DOI: 10.25181/jppt.v20i1.1552
<![CDATA[Cauliflower is a annual vegetable crop that is rich in vitamins and minerals.  This is one of the commodities exported by Indonesia. Fertilization is a determinant of the success of vegetable farming. The use of chemical fertilizers that are not balanced with organic fertilizers can have a negative impact on the environment. Vinasse is an industrial waste that is rich in organic matter, potassium, calcium and micro nutrients and can be used as liquid organic fertilizer. This study aims to determine the effect of giving several doses of vinasse on the growth and production of cauliflower. The experimental design used is a single factor Randomized Block Design (RCBD). The treatment dose used consisted of four levels namely, 0 l / ha (V0), 15000 l / ha (V1), 20000 l / ha (V2), 25000 l / ha (V3). The results showed that there was a very significant effect on plant height at 14 DAP and 21 DAP, and on the variabels of flower weight per sample, flower weight per bed, and fresh stover weight per sample, and showed a significant effect on the number of leaf variabel on age 14 DAP and 21 DAP. The best treatment of vinasse fertilizer is 25000 l / ha (V3).]]>
