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Jurnal Ilmu dan Teknologi Peternakan Tropis

JITPT
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Published by Universitas Halu Oleo

ISSN : 24067489 EISSN : 24069337 DOI : -

Core Subject : Health,

Veterinary

Jurnal Ilmu dan Teknologi Peternakan Tropis (JITRO) adalah jurnal ilmiah mempublikasikan hasil penelitian dan review bidang peternakan.

Arjuna Subject : -

Articles 44 Documents

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Search results for , issue "Vol 9, No 1 (2022): JITRO, Januari 2022" : 44 Documents clear

The Conservation on Indonesian Native Chicken Biodiversity Through Primordial Germ Cells Cryopreservation Yuli Arif Tribudi; Tatan Kostaman
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, Januari 2022
Publisher : Universitas Halu Oleo

ABSTRACT Primordial germ cells (PGCs) can be cryopreserved and used as a potential tool for the preservation of poultry biodiversity. By transplanting donor PGCs into recipient embryos to produce chimera germline, PGCs can migrate to developing gonads where germ cells are produced, thus enabling reproduction of offspring derived from donor PGCs via mating of chimera germline. Collection of PGCs from the circulation of chickens. Fertile eggs were incubated in a portable incubator for 2.5 days. Purification of circulating PGCs with nycodenz. Then the PGCs were frozen using the 5% DMSO cryoprotectant and to determine the success of cryopreservation, the donor PGCs were transplanted into the recipient embryos. The number of circulating PGCs was 50.58 ± 5.79 cells per embryo, the percentage of PGCs viability after thawing was 80.18 ± 1.25%. From the results of the PGCs transplant by looking at the migration of donor PGCs in recipient embryos with PKH-16 staining, it is clear. In conclusion, PGCs cryopreservation in this study can generally support the survival of good chickens and it is hoped that this PGCs conservation protocol can be used for other native chickens.Keywords: PGC, cryopreservation, chicken, conservation

Peran Kelompok Ternak Margo Makmur Mandiri Terhadap Pendapatan Peternak Sapi Perah di Dusun Brau, Kota Batu Anie Eka Kusumastuti; Ridho Bayu Nugroho; Umi Wisaptiningsih
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, Januari 2022
Publisher : Universitas Halu Oleo

Penelitian ini bertujuan untuk mengetahui tingkat pendapatan peternak sapi perah di Kelompok Ternak Margo Makmur Mandiri Dusun Brau, menganalisis peran kelompok ternak sebagai unit usaha, wahana kerjasama dan kelas belajar terhadap pendapatan peternak sapi perah, serta mengidentifikasi faktor-faktor yang mempengaruhi peran kelompok ternak terhadap tingkat pendapatan peternak sapi perah di Kelompok Ternak Margo Makmur Mandiri, Dusun Brau. Metode penelitian yang digunakan yaitu survey dengan observasi secara langsung dan wawancara menggunakan kuesioner terstruktur sebagai alat pengambilan data. Sampel responden yang digunakan peternak sapi perah yang tergabung sebagai anggota aktif dalam Kelompok Ternak Margo Makmur Mandiri sejumlah 50 orang. Data dianalisis dengan menggunakan analisis ekonomi, analisis regresi linier berganda, dan analisis descriptive. Hasil penelitian menunjukkan bahwa rata-rata tingkat pendapatan peternak yang tergabung dalam Kelompok Ternak Margo Makmur Mandiri sebesar Rp. 1.607.798/bulan. Kelompok Ternak Margo Makmur Mandiri dapat berperan dalam meningkatkan pendapatan peternak di Dusun Brau, Kota Batu, Jawa Timur khususnya dalam hal fasilitas kerjasama dengan pihak luar, pertemuaan berkala, dan berkelanjutan, serta fasilitas komunikasi dengan sumber informasi dan teknologi yang disediakan. Kata kunci: peran kelompok ternak, pendapatan peternak sapi perah, peternakan sapi perah The Role of Livestock Group Margo Makmur Mandiri Towards Dairy Farmer’s Income at Brau Village, Batu CityABSTRACTThis research purposes were to find out the income level of dairy farmer’s at Brau Village as well as to analyze the role of livestock group for business unit, cooperation facility, and study group towards dairy farmer’s income at Brau Village, Batu, and to identify factors influencing the role of livestock group to dairy farmers’s income. The research method used was survey method. Data were obtained from direct interview by using structured questionnaire. Sample used in this research determined by purposive sampling with a number of 50 respondents of dairy farmers. Data were analyzed by using economic analysis, multiple regression analysis, and descriptive analysis. The results revealed that the average income of dairy farmers member of Margo Makmur Mandiri livestock group was 1,607,798 IDR per month per farmer. In addition, Margo Makmur Mandiri livestock group can play a role in term of increasing the income of dairy farmer’s especially in cooperation facility with related outsider groups, periodic meetings, and also communication facilities with provided information and technology resources. Keywords: The role of livestock groups, dairy farmer’s income, dairy cattle

Motilitas dan Viabilitas Spermatozoa Sapi Bali Polled dan Bertanduk pada Setiap Tahapan Proses Pembekuan Sri Gustina; Hasbi Hasbi; Herry Sonjaya; Sudirman Baco; Abdul Latief Toleng; Mutmainna Mutmainna; Sitti Farida
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, Januari 2022
Publisher : Universitas Halu Oleo

ABSTRAKSapi bali merupakan sapi yang dikembangkan, dimanfaatkan dan dilestarikan sebagai sumberdaya ternak asli. Saat ini telah dikembangkan populasi sapi bali polled. Sapi bali polled merupakan sapi bali yang tanduknya tidak bertumbuh secara alami. Tujuan dari penelitian ini adalah untuk mengetahui persentase motilitas dan viabilitas spermatozoa sapi bali polled dan bertanduk pada setiap tahapan proses pembekuan. Koleksi semen dilakukan dengan metode vagina buatan. Semen hasil koleksi selanjutnya dievaluasi secara makroskopik dan mikroskopik. Semen yang memenuhi persyaratan diproses lebih lanjut untuk dibekukan. Pejantan yang digunakan sebanyak 3 ekor polled dan 3 ekor bertanduk dengan umur 3,5 sampai 6 tahun. Parameter yang diamati meliputi motilitas dan viabilitas selama proses pembekuan sampai post thawing. Data yang diperoleh diuji dengan uji-t (sampel T-Test independen). Hasil penelitian menunjukkan bahwa tidak ada perbedaan nyata (p>0,05) antara sapi bali polled dan bertanduk terhadap persentase motilitas setelah pengenceran (63,33±1,92 vs 65,00±0,00). Namun, terdapat perbedaan yang nyata (p<0,05) setelah equilibrasi yaitu 54,99±1,35 vs 60,00±0,00 dan post thawing 45,41±0,83 vs 49,58±0,83. Persentase viabilitas setelah pengenceran dan setelah equilibrasi tidak berbeda nyata (p>0.05) yaitu 81,94±4,05 vs 86,51±1,26 dan 72,80±6,80 vs 80,17±2,74. Namun, pada post thawing terdapat perbedaan yang nyata (p<0,05) yaitu 56,95±3,74 vs 72,55±2,00. Kesimpulannya bahwa persentase motilitas dan viabilitas spermatozoa sapi bali polled lebih rendah dibandingkan yang bertanduk.Kata Kunci: motilitas, sapi bali polled, sapi bali bertanduk, spermatozoa, viabilitasMotility and Viability of Spermatozoa of Polled and Horned Bali Bulls at Each Stage of the Freezing Process ABSTRACTBali cattle are cows and bulls that are developed, utilized and preserved as a native livestock resource. Currently, a population of polled bali cattle has been developed. Polled bali cattle are bali cattle whose have horns do not grow naturally. The purpose of this study was to determine the percentage of motility and viability of spermatozoa of polled and horned bali cattle at each stage of the freezing process. Semen collection was carried out by the artificial vaginal method. The collected semen was then evaluated macroscopically and microscopically. The semen that meets the requirements is further processed to be frozen. The bulls used were 3 polleds and 3 horneds with an age of 3.5 to 6 years. Parameters observed included motility and viability during the freezing process until post thawing. The data obtained were tested by t-test (independent sample T-Test). The results of this study indicated that there was no significant difference (p>0.05) between polled and horned bali cattle on the percentage of motility after dilution (63.33 ± 1.92 vs 65.00 ± 0.00). However, there was a significant difference (p<0.05) after equilibration (54.99±1.35 vs 60.00±0.00), and post thawing (45.41±0.83 vs 49.58±0.83). The percentage of viability after dilution and after equilibration was not significantly different (p>0.05), 81.94 ± 4.05 vs 86.51 ± 1.26 and 72.80 ± 6.80 vs 80.17 ± 2.74. However, there was a significant difference in post thawing (p<0.05), 56.95 ± 3.74 vs 72.55 ± 2.00. It can be concluded that the percentage of motility and viability of spermatozoa of polled bali cattle is lower than horned.Keywords: motility, polled bali bull, horned bali bull, spermatozoa, viability

Manajemen Strategis Usaha Peternakan Melalui Metode Force Field Analysis dan Rekomendasi Kebijakan Supardi Rusdiana; Umi Adiati; Anita Hafid; Chalid Talib; Amam Amam
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, Januari 2022
Publisher : Universitas Halu Oleo

ABSTRAK Manajemen strategis difokuskan pada proses penetapan tujuan organisasi, pengembangan kebijakan, perencanaan untuk mencapai sasaran, mengalokasikan sumber daya untuk menerapkan kebijakan, serta merencanakan pencapaian tujuan organisasi. Penelitian bertujuan untuk memproyeksikan manajemen strategis usaha peternakan melalui metoda force field analysis: melalui rekomendasi kebijakan. Penelitian dilakukan di Peternakan Al-Fatih yang merupakan peternakan semi modern dengan sistem syari’ah di Kabupaten Bondowoso Provinsi Jawa Timur. Data diperoleh dengan cara Focus Group Discussion (FGD) dan wawancara secara mendalam (in-depth interview) dengan narasumber utama (key informan). Analisis data menggunakan metode Force Field Analysis (FFA) berdasarkan faktor pendorong (D) dan faktor penghambat (H). Hasil penelitian didapatkan bahwa faktor pendorong pengembangan subsektor peternakan ialah bibit murah dan kualitas genetik bagus, adaptasi bibit terhadap kondisi lingkungan, transparan dan kepercayaan mitra, harga penjualan stabil, potensi eduwisata dan unit pengolahan limbah, serta rekrutmen tenaga kerja muda, sedangkan faktor penghambat pengembangan subsektor peternakan ialah Sumber Daya Manusia (SDM) peternak mitra, modal usaha, daging impor dan sapi bakalan impor, penyakit ternak, dan belum memiliki unit bisnis pengolahan. Kesimpulan penelitian menunjukkan bahwa faktor pendorong (D) memiliki nilai lebih kecil jika dibandingkan dengan faktor penghambat (H), maka rekomendasi kebijakan yang diusulkan ialah menguatkan sistem agribisnis peternakan menuju sistem agroindustri peternakan.Kata Kunci: faktor pendorong, faktor penghambat, agribisnis, agroindusri, peternakan sya’riahStrategic Management of Livestock Business Through Force Field Analysis Method and Policy RecommendationsABSTRACT Strategic management is focused on the process of setting organizational goals, developing policies, planning to achieve goals, allocating resources for implementing policies, and planning for achieving organizational goals. The research aims to project the strategic management of livestock business through force field analysis method: through policy recommendations. The research was conducted at Al-Fatih Farm, which is a semi-modern farm with the syari'ah system in Bondowoso Regency, East Java Province. Data obtained by means of Focus Group Discussion (FGD) and in-depth interviews (with key informants). Data analyzed by Force Field Analysis (FFA) method based on driving factors (D) and inhibiting factors (H). The results showed that the driving factors for the development of the livestock subsector were cheap breed and good genetic quality, adaptation of breed to environmental conditions, transparency and partner trust, stable selling prices, potential for education farm and waste treatment unit, and recruitment of young workers, while the inhibiting factor for the development of the livestock subsector was Human Resources (HR) of partner farmers, business capital, imported meat and imported beef cattle, livestock diseases, and do not yet have a processing business unit. The conclusion of the research shows that the driving factor (D) has a greater value when compared to the inhibiting factor (H). The proposed policy recommendation is to strengthen the livestock agribusiness system towards the livestock agro-industrial system.Keywords: driving factors, inhibiting factors, agribusiness, agroindustry, sya'riah livestock

Revealing the Genetic Mutation in a Diprosopus Monauchenos Calf Widya Pintaka Bayu Putra; Syahruddin Said; Meda Febyastri; Wisnu Krisna Nanditya; Risqa Novita
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, Januari 2022
Publisher : Universitas Halu Oleo

ABSTRACTDiprosopus Monauchenos (DM) is one of congenital defect that can be caused by a genetical factor. This study was conducted to reveal the genetic mutation of DM cattle. Research showed that along 354 bp of partial BTA13 sequence (69,985,393th - 69,985,745th nucleotide) in DM and normal samples were obtained by sequencing analysis. Research showed that one insertion/deletion (indel) mutation at 28th and two transition mutations at 241th and 269th positions were detected in both samples. The obtained sequence in the present study was located at the intronic region of LPIN3 gene (69,972,281th - 69,991,534th nucleotide). In the future, a depth research to investigate the genetic mutation in DM animal with whole genome analysis is important to get the results accurately.Keywords: cattle, DM, mutations, sequencing, BTA13

Profile of Total Leukocytes and Differential Leukocytes in Broilers Using Herbal Feed Additives Achmad Bagus Adhiluhung Mardhotillah; Nur Hidayah; Usman Ali; Osfar Sjofjan; Irfan Djunaidi
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, Januari 2022
Publisher : Universitas Halu Oleo

ABSTRACTThis study aims to determine the effect of herbal plant-based feed additives, namely Aloe vera and turmeric flour on the appearance of total leukocytes and leukocyte differential in broilers. The material used for this research is 64 Day old Chick (DOC). The method used a Completely Randomized Design (CRD) with 4 treatments and 4 replicates which contained 4 heads. Total leukocytes profile and leukocyte differential including eosinophils, basophils, heterophils, lymphocytes, and monocytes were observed in this study. The results showed that the combination of Aloe vera and turmeric flour had a significant effect (p<0.01) on total leukocytes (10.73±0.43). Another result in this study was that the combination of herbal feed additives had no effect (p>0.05) on eosinophils (0.27±0.25) and basophils (0.03±0.05), however heterophile (3.42±0.45), lymphocytes (6.48±0.16), and monocytes (0.54±0.16) showed a significant effect (p<0.05). The conclusion is that the addition of herbal feed additives from Aloe vera flour, turmeric flour, and their combination can improve and enhance immune conditions in terms of the total leukocytes and leukocyte differential profiles in broilers.Keywords: Aloe vera flour, turmeric flour, leukocyte profile, antimicrobial

Perbandingan Daya Tunas, Daya Tetas, dan Karakteristik Kuantitatif Ayam KUB dengan Ayam Kampung (G1) Millisani Utama; Depison Depison; Gushairiyanto Gushairiyanto; Helmi Ediyanto
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, Januari 2022
Publisher : Universitas Halu Oleo

ABSTRAKPenelitian ini bertujuan untuk mengetahui perbandingan daya tunas, daya tetas dan karakteristik kuantitatif ayam KUB dengan ayam kampung (G1) sampai umur 3 bulan. Materi yang digunakan 315 butir telur dan 174 ekor ayam per galur dengan Metode penelitian eksperimen. Data yang dikumpulkan meliputi daya tunas, daya tetas, bobot badan, dan pertambahan bobot badan serta ukuran-ukuran tubuh. Data bobot badan, pertambahan bobot badan, dan ukuran-ukuran tubuh dianalisis menggunakan uji-t, sedangkan vektor rataan ukuran-ukuran tubuh dianalisis menggunakan uji T2-Hotelling. Penciri bentuk dan ukuran tubuh dianalisis menggunakan Analisis Komponen Utama. Hasil penelitian ini menunjukkan bahwa daya tunas ayam KUB dan ayam kampung yaitu 82,86% dan 81,59%, sedangkan daya tetas yaitu 71,26% dan 69,65%. Karakterisitik kuantitatif ayam KUB berbeda nyata p<0,05) lebih tinggi dibandingkan dengan ayam kampung. Penciri ukuran tubuh ayam KUB dan ayam kampung adalah lingkar dada, sedangkan penciri bentuk tubuh adalah panjang tubuh atas dan panjang sayap. Respon seleksi Ayam KUB dan ayam kampung yaitu 7 gr dan 9,3 gr. Kesimpulan: Ayam KUB dan ayam kampung memiliki daya tunas dan daya tetas yang relatif sama. Karakteristik kuantitatif ayam KUB lebih baik dibandingkan dengan ayam kampung. Ayam KUB dan ayam kampung memiliki respon seleksi yang positif.Keywords: Ayam KUB, ayam kampung, G1, respon seleksiComparison of Fertility, Hatchability and Quantitative Characteristics between KUB Chicken and Kampung Chicken (G1)ABSTRACTThis study aims to determine the comparison of fertility, hatchability, and quantitative characteristics of KUB chickens with kampung chickens (G1) 3 months of age. The material used is 315 eggs and 174 chickens per strain and using experimental methods. The data collected fertility, hatchability, body weight, body weight gain, and body measurements. Data on body weight, weight gain, and body measurements were analyzed using a t-test, while the mean vector of body measurements was analyzed using test T2-Hotelling. Body shape and size characteristics were analyzed using Principal Component Analysis. The results of this study showed the fertility of KUB and kampung chicken is 82.86% and 81.59%, while the hatchability is 71.26% and 69.65%. The quantitative characteristics of KUB chickens are significantly (p<0,05) higher than those of kampung chickens. The body size characteristics of KUB and kampung chickens are chest circumference, while the shape characteristics are back length and wing length. The selection response of KUB and kampung chicken is 7 g and 9.3 g. Conclusion: KUB chickens and kampung chickens have fertility and hatchability relative same. The quantitative characteristics of KUB chickens are better than of kampung chickens. KUB and kampung chickens have a positive selection response.Keywords: KUB chickens, kampung chickens, G1, selection responses

Fungi Mikoriza Arbuskula pada Rhizosfer Rumput Mulato (Brachiaria hybrid cv. Mulato) La Malesi; Indrayani Indrayani; Husna Husna; Syamsuddin Syamsuddin; Rahman Rahman
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, Januari 2022
Publisher : Universitas Halu Oleo

ABSTRAKRumput mulato tumbuh subur pada lahan-lahan marginal, tanah yang unsur hara sangat rendah. Kemampuan rumput mulato tumbuh dengan subur pada lahan tersebut menandakan adanya unsur lain yang bersimbiosis dengannya. Kemungkinan yang menyebabkan rumput mulato tumbuh dengan baik adalah keberadaan mikoriza yang mengkoloni akar atau yang berada pada rhizosfer. Penelitian bertujuan untuk mengekspolorasi dan mengidentifikasi jenis fungi mikoriza arbuskula pada rhizosfer rumput mulato. Sampel tanah dan akar rumpu mulato diambil dilahan laboratorium lapangan Fakultas Peternakan Universitas Halu Oleo. Analisis sampel tanah untuk mengekspolorasi dan mengidentifikasi jenis fungi mikoriza arbuskula dilakukan di pusat penelitian Biologi LIPI Bogor. Hasil penelitian menunjukkan bahwa pada rhizozfer rumput mulato ditemukan 5 jenis FMA yang berasal dari 3 genus mikoriza misalnya glomus sp, gigaspora sp, dan acaulospora sp. Jenis glomus sp ditemukan sejumlah 7 spora, giga spora gregaria ditemukan sejumlah 1 spora. Sedangkan jenis gigaspora gregaria N.C. Schenck dan T.H. Nicolson ditemukan sejumlah 13 spora. Acaulospora tuberculata Janos dan Trappe diemukan sejumlah 4 spora, sedangkan acaulospora scrobiculata Trappe ditemukan sejumlah 1 spora. Pada rhizosfer rumput mulato terjadi kolonisasi mikoriza karena ditandai dengan adanya struktur FMA. Struktur FMA yang ditemukan adalah hifa eksternal, hifa internal, vesikula dan arbuskula. Persentase kolonisasi FMA pada akar rumput mulato yakni 49-88% atau rata-rata sekitar 72,87%.Kata Kunci: Mikoriza arbuskula, glomus, Brachiaria hybrid cv. MulatoExpoloration Arbuscular Mycorrhizal Fungi in Rhizosphere Mulato Grass (Brachiaria hybrid cv. Mulato)ABSTRACTMulato grass thrives on marginal lands, very low nutrients soils. The ability of mulato grass to thrive on the land indicates the presence of other elements that symbiosis with it. The possibility that causes mulato grass to grow will is the presence of mycorrhizal that colonizes the roots or that resides in the rhizosphere. The study aimed to explore and identify the type of arbuscular mycorrhizal fungi in the rhizosphere of mulato grass. Soils and roots samples mulato grass were taken at the field laboratory of the Faculty of Animal Science, Halu Oleo University. Analysis of soil samples to expolore and identify the type of arbuscular mycorrhiza fungi was conducted Biology research center LIPI, Bogor. The results showed that in rhizosphere mulato grass 5 types were found of AMF derived genera three of mycorrhizal such as glomus sp, gigaspora sp and acaulospora sp. The glomus sp types found 7 spores number. Gigaspores gregaria found 1 spores number. The gigaspores gregaria N.C Schenck and T.H. Nicolson found 13 spores number. The acaulospora tuberculata Janis and Trappe found 4 spores number. While acaulospora scrobiculata Trappe found 1 spores number. In the rhizosphere of mulato grass there are colonization at mulato grass roots 49-88% ranges with 72,87% average. Keywords: Mycorrhiza arbuskula, glomus, Brachiaria hybrid cv. Mulato

Prevalensi Penyakit Baliziekte pada Sapi Potong di Kabupaten Majene, Sulawesi Barat Deka Uli Fahrodi; Nur Saidah Said; Ferbian Milas Siswanto; Hendro Sukoco
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, Januari 2022
Publisher : Universitas Halu Oleo

ABSTRAKTujuan dari penelitian ini adalah untuk menggambarkan prevalensi penyakit Baliziekte pada sapi potong di Kabupaten Majene, Sulawesi Barat. Penelitian ini menggunakan metode penelitian kuantitatif dengan pendekatan analisis data sekunder. Data kasus kejadian Baliziekte pada sapi potong di Kabupaten Majene, Sulawesi Barat pada tahun 2018-2020 didapatkan dari Dinas Pertanian, Peternakan dan Perkebunan Kabupaten Majene. Hasil analisis menunjukkan bahwa prevalensi penyakit Baliziekte pada sapi potong di Kabupaten Majene mengalami peningkatan dalam rentang waktu dua tahun terakhir. Jumlah prevalensi penyakit Baliziekte pada tahun 2018 adalah 0,789 %, pada tahun 2019 terjadi penurunan menjadi 0,716 % dan meningkat pada tahun 2020 menjadi 1,257 %. Kecamatan Sendana merupakan satu-satunya daerah dengan peningkatan jumlah kasus Baliziekte pada sapi potong setiap tahunnya, sedangkan Kecamatan Banggae Timur mengalami penurunan jumlah kasus Baliziekte yaitu dari prevalensi 0,078% di tahun 2018 menjadi 0% di tahun 2019 dan 2020. Kecamatan Tubo Sendana merupakan daerah dengan jumlah kasus dan prevalensi tertinggi pada tahun 2020, sedangkan Kecamatan Banggae dan Pamboang merupakan daerah dengan data kasus dan prevalensi 0%. Kata kunci: sapi potong, prevalensi, baliziekte, Kabupaten MajeneThe Prevalence of Baliziekte Disease in Beef Cattle in Majene, West SulawesiABSTRACTThe purpose of this study was to illustrate the prevalence of Baliziekte disease in beef cattle in Majene, West Sulawesi. This study was quantitative research by analyzing the secondary data analysis approach. Data on the Baliziekte incidence in beef cattle in Majene, West Sulawesi in 2018-2020 were obtained from the Department of Agriculture, Livestock and Plantation of Majene Regency. The results of the analysis showed that the prevalence of Baliziekte disease in beef cattle in Majene Regency has increased in the last two years. The prevalence of Baliziekte disease in 2018 was 0.789%, decreased to 0.716% in 2019, and increased sharply to 1.257% in 2020. Sendana District was the only area with an increase in the number of Baliziekte cases in beef cattle every year, while the East Banggae District underwent depletion in the number of Baliziekte cases, from a prevalence of 0.078% in 2018 to 0% in both 2019 and 2020. Tubo Sendana District was the area with the highest number of cases and prevalence in 2020, while the Districts of Banggae and Pamboang were both maintained a 0% case and prevalence throughout the years.Keywords: beef cattle, prevalence, baliziekte, Majene

Deteksi Gen Penyandi Resistansi blaTEM, blaSHV, dan blaCTXM pada Pseudomonas aeruginosa Ayam Petelur di Kabupaten Cianjur, Jawa Barat Safika Safika; Fauzan Arisandi; Fachriyan Hasmi Pasaribu; Yamin Yaddi
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, Januari 2022
Publisher : Universitas Halu Oleo

ABSTRAKPseudomonas aeruginosa merupakan bakteri oportunistik patogen yang mampu meninfeksi bagi hewan dan manusia. Resistansi terhadap banyak antibiotik memberikan tantangan yang cukup besar dalam pengobatan infeksi Pseudomonas aeruginosa. Penelitian ini bertujuan mendeteksi adanya resistansi antibiotik dan gen penyandi resistansi pada isolat bakteri Pseudomonas aeruginosa yang diisolasi dari peternakan ayam petelur di Kabupaten Cianjur, Jawa Barat. Sampel diisolasi dan identifikasi sebanyak enam puluh enam melalui usap kloaka. Sampel yang dikoleksi dilakukan kultur pada media selektif (MacConkey agar), dilanjutkan dengan uji mikroskopik, uji biokimia, dan dikonfirmasi dengan secara molekuler dengan polymerase chain reaction (PCR). Sampel yang positif diuji kepekaan terhadap antibiotik menggunakan metode Kirby-Bauer disk diffusion dan mendeteksi gen penyandi resistansi. Hasil penelitian 8 sampel bakteri Pseudomonas aeruginosa dilakukan uji kepekaan antibiotik menunjukkan tingkat resistansi terhadap golongan antibiotik beta laktam (ampisilin 75%) dan aminoglikosida (gentamisin 0%). Dekteksi gen penyandi resistansi secara berturut-turut menunjukkan gen blaTEM (100%), blaCTXM (100%) terdeteksi, sedangkan gen blaSHV tidak terdeteksi pada isolat yang diuji. Perlunya dilakukan penelitian lanjutan untuk mendeteksi sampel dari lingkungan, tempat air minum, pakan maupun karyawan di peternakan tersebut. Sehingga memeberikan informasi dan kajian ilmiah untuk pengaturan regulasi penggunaan antibiotik di peternakan.Kata Kunci: antibiotik, ayam petelur, gen resisten, Pseudomonas aeruginosaDetection of blaTEM, blaSHV, and blaCTXM Resistance Coding Genes in Pseudomonas aeruginosa Layer Chickens in Cianjur Regency, West JavaABSTRACTPseudomonas aeruginosa is a pathogenic opportunistic bacteria capable of infecting animals and humans. Resistance to many antibiotics presents considerable challenges in the treatment of Pseudomonas aeruginosa infections. This study aims to detect the presence of antibiotic resistance and genes encoding resistance in isolates of Pseudomonas aeruginosa isolated from laying hens in Cianjur Regency, West Java. Sixty-six samples were isolated and identified through cloacal swab. The collected samples were cultured on selective media (MacConkey agar), followed by microscopic tests, biochemical tests, and confirmed molecularly by polymerase chain reaction (PCR). Positive samples were tested for susceptibility to antibiotics using the Kirby-Bauer disk diffusion method and detected genes encoding resistance by PCR. The results of the study of 8 samples of Pseudomonas aeruginosa bacteria were tested for antibiotic sensitivity showing the level of resistance to beta-lactam antibiotics (ampicillin 75%) and aminoglycosides (gentamicin 0%). The detection of resistance coding genes, respectively, showed that blaTEM (100%), blaCTXM (100%) genes were detected, while the blaSHV gene was not detected in the tested isolates. Further research is needed to detect samples from the environment, drinking water, feed and employees on the farm. So that it provides information and scientific studies to regulate the regulation of the use of antibiotics in livestock.Keywords: antibiotic, laying hens, Pseudomonas aeruginosa, resistant genes

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