Article Per Year (5 Year)
p-Index From 2019 - 2024
7.974
P-Index
This Author published in this journals
All Journal Jurnal Penelitian dan Pengembangan Pendidikan Bioscience Bioeducation Journal Jurnal Pendidikan Biologi Indonesia Bio Ilmi: Jurnal Pendidikan Jurnal Pendidikan Biologi Jurnal Penelitian Pendidikan IPA (JPPIPA) PENDIPA Journal of Science Education Diklabio: Jurnal Pendidikan dan Pembelajaran Biologi Biodik : Jurnal Ilmiah Pendidikan Biologi Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi Jurnal Tarbiyah : Jurnal Ilmiah Kependidikan Journal on Education Bioedusiana: Jurnal Pendidikan Biologi Atrium Pendidikan Biologi Jurnal Mangifera Edu Jurnal Pendidikan dan Konseling Penbios: Jurnal Pendidikan Biologi dan Sains Jurnal Ilmiah Profesi Pendidikan Jurnal METAEDUKASI AS-SABIQUN: Jurnal Pendidikan Islam Anak Usia Dini Journal On Teacher Education (Jote) Sustainable: Jurnal Kajian Mutu Pendidikan Bioeduca : Journal of Biology Education Journal of Digital Learning and Education Ta'dib Al-Alam : Islamic Natural Science Education Journal Ta'dib Jurnal Esabi (Jurnal Edukasi dan Sains Biologi) Symbiotic: Journal of Biological Education and Science Jurnal Serambi Biologi
Zulyusri, Zulyusri
Universitas Negeri Padang
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Physics Public Health Social Sciences Transportation Other

Published : 63 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : Jurnal Serambi Biologi

 1 
Specific Primer Design and Optimization of Monodehydroascorbate reductase (MDHAR) Gene Amplification in Rice (Oryza sativa L.) Jumatul Hafsah; Afifatul Achyar; Zulyusri; Yusni Atifah; Linda Advinda; Violita
Jurnal Serambi Biologi Vol. 8 No. 3 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Monodehydroascorbate reductase (MDHAR) is an enzyme responsible for growth and response to biotic and abiotic stress. MDHAR in rice shows a higher sensitivity to stress compared to other plants. This study aims to obtain specific primers for the MDHAR gene in rice to be used in PCR amplification so that it can amplify the MDHAR gene. Primers are designed using the Pickprimer and Geneious Primer tools. Optimization of annealing temperature was carried out using the gradient PCR method and then an in vitro primary specification test was carried out using the Touchdown PCR method. The results of the primary design obtained one candidate primer that met the ideal primer requirements, namely a pair of primers (5'-AAAAACACTGCATGGGTCGTC-3' and 5'-CGCCTACCGTTTCCCAAGTT-3') with an amplicon length of 160 bp. The visualization results of PCR products using 1.5% agarose showed that 6 samples were able to amplify the MDHAR gene at 160 bp in size. However, in each lane there is a non-specific DNA band (Primer dimer). In vitro primer specification testing with Touchdown succeeded in increasing product formation specifications and was able to reduce non-specific DNA bands (Primer dimers).
Co-Authors Abdul Rahman Abdul Razak Abi Suwars Afifatul Achyar Ahmad Subari Ahmad Syukri Al Fahrurz Ardi Ardi Asra Indah Asra Indah Atifah, Yusni Azwir Anhar Azwir Azwir Cindy Oktafina Nengsih Cindy Oktafina Nengsih Desi Wahyuni Elismawati Elismawati Elmayana Fradila Elsa Yuniarti Fadika Hayyuni Festiyed Fitri Arsih Fitri Arsih Hadeli Hadeli Heffi Alberida Helendra . Helka Yuliati Helsa Rahmatika Hidayat Al Azmi Ida Elfira Ilwandri Ilwandri Indra Hartanto Irdawati Irdawati Isnaini Nur Habibah Jumatul Hafsah Khairatunnisa Khairatunnisa Khodzijah Nur Amalia Kurnia Wulansari Laila Kurnia Sari Lenti Supriwardi Linda Advinda Lucy Petra Kamelsa Lufri Lufri Melandi Wimudi Melitha Aurora Hasanti Mico Arisanto Muhamad Sholichin Nafisha Vebiola Irani Nanta Mulia Nella Fauziah Nindya Ananda Latifa Nining Efriani Nisa Al Mukarromah Nisa Firdha Nisrina Zakia Khalel Noni Fatillah Noveridha Utama Novi Indah Lestari Novia Afriliska Novica Ashari Pingki Lestari Putri Sarinami Rahma Lestari Rahmadhani Fitri Rahmawati Darussyamsu Rahmawati Rahmawati Rahmi Aulya Rahmi Suci Nadira Rahmita Mawadah Ramadhan Sumarmin Relsas Yogica Restu Fitriani Retti Yuselmi Retti Yuselmi Revi Gina Gunawan Revi Gina Gunwan Rhavy Ferdyan Ristiono Ristiono Sa'diatul Fuadiyah Sa’diatul Fuadiyah Selaras, Ganda Hijrah Selvi Seftiani Septu Yoelianty Sukma Sintia Putri Suhaimi Suhaimi Tiffany Mantoviana Tomi Apra Santosa Tomi Apra Santosa Tomi Apra Santosa Santosa Upris Yastati Violita Violita Violita Weni Febrianti Wirda Taufik Wuri Julita Yayat Suharyat Yosi Laila Rahmi Yulia Adytia Putri