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Fermentasi Nira Nipah Menjadi Bioetanol Menggunakan Teknik Immobilisasi Sel Saccharomyces Cerevisiae Ella Awaltanova; Syaiful Bahri; Chairul Chairul
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 2, No 2 (2015): Wisuda Oktober Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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The high dependence of fuel resource such as oil, coal, and gas will influence depletion of fossil resources (oil, natural gas, and coal). Therefore, it is important to study conversion of biomass to bioethanol as renewable energy to overcome dependence on fossil fuels. Production of bioethanol using free cell in fermentation and it several disadvantages in separation of yeast cells and biethanol concentration. This research was conducted the fermentation of nypa sap with immobilization cell, using tween 80 and ergosterol as suplement to obtain high concentration of bioethanol as well as avoid osmosis in cells . This work is aimed to study of bioethanol production from nypa sap using immobilized cells, to determine effect of cell immobilization weight and fermentation time to bioethanol, and to obtain maximum conditions on the process. Fermentation was conducted in 2L fermentor having variations of fermentation time such as 24, 36, 48, 60, 72, 84, and 96 hours respectively and cell immobilization weight e.g. 5 %w/v, 10 %w/v, 15 %w/v, and 20 %w/v respectively, and also addition of tween 80 of 0,2% v/v and ergosterol of 0,5 g/l. The concentration of bioethanol was increased with increases of immobilized cells. Maximum conditions of bioethanol production from nypa sap using immobilized cells are shown having cell immobilization weight of 20% w/v and fermentation hours 96th about 17,574 % v/v equal with 138,708 mg/ml. Keywords : bioethanol, ergosterol, immobilized cells, nypa, sacharomyces cereviceae, tween 80
Pembuatan Bioetanol Dari Nira Aren Secara Fermentasi Menggunakan Yeast Saccharomyces Cerevisiae Dengan Variasi Konsentrasi Inokulum Dan Waktu Fermentasi Elia Simanjuntak; Chairul Chairul; Maria Peratenta Sembiring
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Bioethanol is one of the alternative energy source that can replace fossil energy sources. Bioethanol is biochemistry fluid from fermentation process of sugar by using microorganisms. One material which has potential as raw material for bioethanol production is a palm juice which is economically valuable products of the sugar plant. Palm juice is used as raw material for ethanol because it contains sugar which is large enough around 12.04%. This research aimed to produce bioethanol with various concentration of inoculum 10%, 12.5%, 15% and 17.5% and fermentation time 24, 48, 72 and 96 hours at pH optimum 5. The process of fermentation using Saccharomyces cerevisiae yeast. The sample analysed by using alcoholmeter. From the research results, obtained the highest ethanol concentration of 8% (v/v) at 17.5% inoculum concentration variation with fermentation time 72 hours.Keyword: Bioethanol, Fermentation, Palm Juice, Saccharomyces cerevisiae
Pengaruh Konsentrasi Tween 80 Pada Sakarifikasi Dan Fermentasi Serentak Pati Sorgum Menjadi Bioetanol Ajma Nouri; Chairul Chairul; Silvia Reni Yenti
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 2, No 2 (2015): Wisuda Oktober Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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The growing energy needs of Fuel (BBM) in the world are experiencing the limitations of the natural resources of raw materials in the form of fossil fuels are declining. To meet the needs of fuel need to be developed for non-fossil fuels. One type of vegetable fuel (BBN) is bioetanol. Raw materials that are potentially as bioetanol one is sweet sorghum (Sorghum bicolor l. Moench). Starch sorghum can be converted into glucose and then by using the help of microorganisms are converted into bioetanol. The methods used in this research is the process of sakarifikasi and the fermentation is done simultaneously (SSF) in 2-Litre fermenters. Sampling is conducted during the process of hidrolisa by using StargenTM enzyme and fermentation at specific time intervals to analyze levels of ethanol and sugar levels. Test your sugar levels reduction done by the method of Nelson-Somogy, alcohol concentration testing using the tool alkoholmeter. The purpose of this study was to determine the influence of the concentrations of Tween 80 and the fermentation time against levels of ethanol produced. Starch fermentation process conditions of sorghum with the variation of the concentrations of Tween 80 (0.5%; 1%; 0.9%; 2% and 2.5%) at each sampling time (36; 24; 48; 60, 72 and 96 h). The fermentation process takes place in the anaerobic conditions of operation pH (4.5) speed of 200 rpm and pengadukkan at room temperature. The best fermented indicated on condition of addition of enzyme concentration of 2.5%, the fermentation time 72 hours with the resulting ethanol concentration of 8% (v/v).Keywords: Bioetanol, Fermentation, starch sorghum, StargenTM 002, Tween 80
Variasi Pengadukan Dan Waktu Pada Pembuatan Bioetanol Dari Pati Sorgum Dengan Proses Sakarifikasi Dan Fermentasi Serentak (SSF) Maulia Rayana; Chairul Chairul; Hafidawati Hafidawati
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 1, No 1 (2014): Wisuda Februari Tahun 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Sorghum is one of the agricultural commodities used for biofuels. Sorghum starch characteristics of high carbohydrate and contains high levels of starch as raw material potential of bioethanol. Making bioethanol done with simultaneous saccharification and fermentation process using yeast enzymes StargenTM 002 and Saccharomycess Cereviciae with varying speed agitation of 200, 250, 300 and 350 rpm and fermentation time 12, 24, 48, and 72 hours. The results of the analysis using a spectrophotometer and Alkoholmeter with highest bioethanol yield at speed agitation 350 rpm at 42 hours of fermentation with ethanol content of 8% (v/v) Keyword: Sorghum, Bioethanol, Enzym StargenTM 002, SSF, Speed Agitation
Pengaruh Konsentrasi Gula Dan Waktu Fermentasi Terhadap Fermentasi Asam Asetat Menggunakan Kulit Nanas Dan Acetobacter Aceti Ahmad Dedi Fadillah; Chairul Chairul; Cory Dian Alfarisi
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Pineapple (Ananas comosus (L) Merr) is a fruit which has a golden yellow color. Pineapple can thrive in tropical climates such as in Indonesia. Pineapple production in Indonesia in 2009 was 1,558,196 tons. The sugar contained in pineapple is 2.32% glucose, 1.42% fructose, and 7.89% sucrose. So that it can be used as an alternative material for the production of acetic acid. Acetic acid can be produced from raw materials containing starch or sugar by fermentation using microorganisms. The purpose of this study was to determine the effect of fermentation time and sugar concentration on the yield of acetic acid produced and to find out the optimal time and concentration of sugar in pineapple rind fermentation into acetic acid using Saccharomyces cerevisiae and Acetobacter aceti bacteria. The initial sugar concentration variations were 10%, 20%, and 30%, while the fermentation time was 2, 3, 4, 5, 6 days. The acetic acid fermentation process was carried out at pH 6, stirring speed 200 rpm and 10% inoculum. The test results using the Nelson-Samogyi method showed that the highest sugar concentration was 30% (174,678 g/L) of sugar added to pineapple rind raw material. The highest concentration of acetic acid was obtained 35.581 g/L and the yield of 30.555%. The best fermentation time is the 6th day of fermentation.Keywords: Acetobacter aceti, acetic acid, fermentation, inoculum, pineapple
Pengolahan Limbah Tekstil Artifisial (Zat Warna Reaktif) Dengan Proses Oksidasi Katalitik Menggunakan Nano-Mn/Carbon Sphere Sri Meilani; Edy Saputra; Chairul Chairul
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 4, No 1 (2017): Wisuda Februari Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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The latest innovations in the processing of textile waste is using advanced oxidation processes (AOPs) by oxidant capable of generating a radical sulphate (SO4*). The purpose of this research is to determine catalyst activity of Nano-Mn/CS in oxidation process, reduce the dye on using peroxymonosulfate and Nano-Mn/CS, and determine the optimum conditions for reducing the dye in water. Catalytic synthesis process carried out by the hydrothermal process at temperature of 180o C for 18 hours in an autoclave to produce black carbon from D-glucose solution and then it will be impregnated with a variation of 3% and 5% Mn. Then it calcined with N2 at temperature of 500o C for 4 hours. The optimum condition for degradation of methylene blue (artificial wastes) 25 ppm carried out for 120 minutes with 0,2 gr/L catalysts Nano-Mn/CS and 4 gr/L oxone with efficiency of 91.07%.Keywords: AOPs, Methylene blue, Nano-Mn/CS, Peroxymonosulfate
Konversi Pati Sorgum Menjadi Bioetanol Menggunakan Variasi Konsentrasi Enzim Stargen tm 002 Dan Yeast Saccharomyces Cerevisiae Dengan Proses Sakarifikasi Dan Fermentasi Serentak Ilham Ilham; Chairul Chairul; Yelmida Azis
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 3, No 1 (2016): Wisuda Februari Tahun 2016
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Abstract

Sorghum is a resource of grain yield with 55-75% starch composition similar to corn, that was potential as raw material for production of bio ethanol. Research based sorghum bio ethanol manufacture carried out using enzymes stargenTM 002 and starch raw materials. The purpose of this study was to determine the concentration of substrate and the best time of fermentation to produce bio ethanol. The research method consists of hydrolysis and saccharification processes are aided by enzymes stargenTM 002 followed by a fermentation process using yeast Saccaromyces Cerevisiae, ferment at room temperature, pH 4.5, enzymes stargenTM 002 concentration 2,5% and evaporated at a temperature 1000C. Using enzyme concentration were 4%, 5%, 6% and 7%, particle size used was 60-80 mesh and time of fermentation 30, 36, 42, 48, 54, 60, 66 and 72 hours. At 6% concentration of enzyme and the optimum time is 60 hours can produce the highest bio ethanol concentration of 12.413% (v/v).Keyword: Bioethanol, Enzym StargenTM 002, Fermentation, Sorghum, Substract Concentrations.
Inaktivasi Termal Spora Bacillus Subtilis Dalam Jus Nanas Khaira Khaira; Evelyn Evelyn; Chairul Chairul
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Bacillus subtilis is a spore-forming bacterium and has been identified as spoilage in fruit-based medium such as pineapple. Food preservation by thermal processing is a conventional method and is still used today. The purpose of this study was to determine the effect of temperature (T: 90 °C, 95 °C, 100 °C) and pH(2 and 6) on the thermal inactivation of B. subtilis spores. It was shown that higher temperature resulted in the decreasing of the time needed to inactivate the spores. Higher resistance of the spores were observed at higher pH. The D-values obtained at pH 2 and temperatures of 90, 95, 100 °C were 11,24; 5,85, and 2.34 minutes, respectively. D-values at pH 6 were; 38,46, 13,70 and 3,55 minutes. The z-values obtained were in the range 9-14 °C. The results of this study indicate the importance of temperature and pH for heat resistance of B. subtilis spores in pineapple juice. Keywords: Bacillus subtilis, Inactivation, Pineapple juice, Resistance, Spores
Penentuan Massa dan Waktu Kontak Optimum Adsorpsi Karbon Aktif Dari Ampas Tebu sebagai Adsorben Logam Berat Pb Riki Irwandi; Silvia Reni Yenti; Chairul Chairul
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 2, No 2 (2015): Wisuda Oktober Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Bagasse is one of the agricultural or industrial waste that can pollute the environment. One effort to improve the economic value of the bagasse can be done with the process into activated carbon. This study aimed to examine the mass effect of activated carbone and the time contact of the bagasse activated carbon adsorption used for heavy metal Pb adsorbent, and to study the characteristics of the activated carbon produced, among others, ash content and moisture content. This research was conducted in three phases were the raw material preparation, the manufacture of activated carbon, and the adsorption of Pb in PbSO4 solution. Variations in the mass carbone used in this study were 1; 2; and 3 gram. As for the time variation adsorption used were 30; 60; 90; and 120 minutes. Percent adsorption is best obtained when the mass of activated carbone of 3 grams and the optimum time contact of 90 minutes is equal to 94,15 %.Keywords: adsorbent, bagasse, activated carbone, mass of activated carbone, adsorption time
Pemurnian Bioetanol Hasil Fermentasi Kulit Nanas Menggunakan Proses Distilasi - Adsorpsi Pada Variasi Jenis Perlakuan Dan Ukuran Pori Adsorben Diah Pramushinta; Said Zul Amraini; Chairul Chairul
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 1, No 1 (2014): Wisuda Februari Tahun 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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One of the alternatives is by utilizing bioethanol as a renewable alternative energy source. This study aims to obtain ethanol from pineapple feel using solid state fermentation process and the purification using distillation and adsorption process, obtain comparative data on the adsorption capacity of the natural zeolite without activation and the activated natural zeolite, to get data the optimum of adsorbent to produce bioethanol, and characterize the physical properties of bioethanol and qualitative test by GC-MS. The pore size of adsorbent variations are 60; 100; 200 mesh and variation of treatment of adsorbent the natural zeolite without activation and the activated natural. From the research is obtained the highest bioethanol concentration average is 10,44 % volume. The most effective process for ethanol purification is distillation and adsorption process by natural zeolite adsorbent without activation with pore size of 200 mesh. Ethanol content increased from 95 % to 99.8 % v. Bioethanol from distillation and adsorption process in accordance with ISO standards.  Keywords: Bioethanol; solid state fermentation; zeolite; distillation and adsorption process
Co-Authors Ade Ayu Oksari Ade Sri Umaiyah Adriani Lestari Adrianto Ahmad Ahmad Dedi Fadillah Ahmad Mursyid Ajma Nouri Alya Alissa Amun Amri ANDRIA AGUSTA Annisa Novianti Samin Anthoni Agustien Arfa Dinata Ageng Tirtayasya Cece R Cory Dian Alfarisi David Hamonangan Deasy Rahmayuni Desi Heltina Deswati Deswati Dewi Kusuma Nurmalasari Diah Pramushinta Dike Putra Ganda Dina Citra Naomi Dina Citra Naomi Harianja Dina Remina Drastinawati Drastinawati Edy Saputra Efrizal Efrizal Eka Trisnawati Elia Simanjuntak Ella Awaltanova Erizal Mukhtar Evelyn Evelyn Farhiz Lagan Fariez Fariez Ferry Lismanto Syaiful Futhanul Wewe Gilang Fathurrahman Gusna Merina Hafidawati, Hafidawati Hauriah Hauriah I Gusti Bagus Wiksuana Ihsanul Khairi Iislamiyah Iislamiyah Imron Sutiono Indra Junaidi Zakaria Intan Oktaviani Irdoni HS Irdoni Irdoni Irdoni S Irvan Fadli Wanda Karim Abdullah Khaira Khaira M Ridwan Afitra Maria Peratenta Sembiring Maulia Rayana Mery Sukmiwati Mohammad Rezky Muhammad Rivai Multi Handriyani Nadhira Iffah Lisandra Nurainas Nurainas Nurmiati Nurmiati Nuvicha Rizqi Yuniva Zikra Oci Khairani Rafly Rafly Reni Kuswanazia Reni Oktaviani Rian Efrizal Riki Ariyadi Riki Irwandi Rizka Sefmaliza Roni Roni Rusnam Rusnam Said Said Said Zul Amraini Shintia Oktaviani Silvia Reni Yenti Sisi Oktadira Kalpatari Sri Budi Sulianti Sri Meilani Sri Rezeki Muria Sunarno Sunarno Suwirmen, Suwirmen Syafira Junia Ardhani SYAIFUL BAHRI Syelvia Putri Utami Tafrikhatul Walidah Tantri Wilinda Julia Tiara Tiara Tri Murningsih Wahyu Mey Riswanto Winarto Tarigan Wisrayetti Wisrayetti Yelmida Azis Yeni Rizki Yolanda Amalia YULIASRI JAMAL Yuthia Aulia Riani ZA ZA Zozy Aneloi Noli Zuherti Zainul Zuhri Syam