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Pemanfaatan Fungi Mikoriza Arbuskula dan Pot Organik untuk Meningkatkan Pertumbuhan Suren (Toona sinensis Roem.) di Persemaian Permanen IPB Dramaga Sri Wilarso Budi; Dwi Puspasari Arum Ramadhani
Jurnal Silvikultur Tropika Vol. 11 No. 2 (2020): Jurnal Silvikultur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.11.2.102-108

Abstract

The cultivation of seedling which uses polybag caused the increase of polybag demand. Due to the rising demand of polybag, the rate of pollution caused by polybag waste is increasing as well, so that it requires an alternative to solve the problem by using organic based pot which is environmentally friendly and easily degraded. This study aimed to analyze the effect of Arbuscular Mycorrhizal Fungi (AMF) adding and the utilization of organic based pot to the growth of Suren (T. sinensis) seedling. This study was conducted using completely randomized design with two factor method, as well as observation for 16 weeks. The results showed that the interaction of organic pot composition treatment and AMF addition were significantly affected to the both parameters of height and diameter, while the treatment of organic pot composition was significantly affected to the parameter of total dry weight, moreover the treatment of AMF addition was significantly affected to the parameter of colonization of AMF. The results of this study indicated that the treatment of newspaper: rice husk gave better response of growth than other treatments. Keywords: Arbuscular Mycorrhizal Fungi, suren (T. sinensis), organic pot
Pemanfaatan Fungi Mikoriza Arbuskula (Fma) Pada Pot Organik Untuk Meningkatkan Pertumbuhan Kayu Afrika (Maesopsis eminii Engl) di Persemaian Permanen Dramaga Sri Wilarso Budi; Maryani Fatimah Putri Hardhani
Jurnal Silvikultur Tropika Vol 11 No 3 (2020): Jurnal Silvikutur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.11.3.126-131

Abstract

The increase cultivation of tree causes the great number of polybags used. To reduce the polybag, the organic pot based on the organic materials that is environmentally friendly is able to be implemented. This study aimed to analyze the effect of organic pot with the addition of Arbuscular Mycorrhizal Fungi (AMF) to develop the growth quality of Maesopsis eminii seedling. This study used the completely randomized design with two factors method. The results of this study showed that the interaction of organic pot treatment and the addition of Arbuscular Mycorrhizal Fungi were significantly affected on the height of Maesopsis eminii seedling. A single treatment of Arbuscular Mycorrhizal Fungi (AMF) addition was significantly affected on the root dry weight as well as the percentage of Arbuscular Mycorrhizal Fungi (AMF) colonization on the root of Maesopsis eminii seedling. The composition of organic pot of newspaper:rice husk with the addition of Arbuscular Mycorrhizal Fungi was the best treatment to enhance the growth of Maesopsis eminii seedling. Keywords: Arbuscular Mycorrhizal Fungi (AMF), kayu afrika (Maesopsis eminii Engl), Organic Pot
Pengaruh Wadah Semai Berbahan Dasar Organik dan Fungi Mikoriza Arbuskula (FMA) Terhadap Pertumbuhan Semai Balsa (Ochroma bicolor Rowlee) Sri Wilarso Budi; Rahmawati Rahmawati
Jurnal Silvikultur Tropika Vol. 11 No. 3 (2020): Jurnal Silvikutur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.11.3.148-153

Abstract

Balsa (Ochroma bicolor Rowlee) are generally sown using a polybag. Polybag made from plastic that is not easily destroyed or degraded so that it can cause problems for the environment. As an alternative, polybag can be replaced by using organic pot. This research aims to analyze the effect of the treatment of raw materials of organic pot and inoculation Arbuscular Mycorrhizal Fungi (AMF) towards growth of balsa (O. bicolor). This study used Completely Randomized Design (CRD) with two factors. The results showed that treatment of organic pot composition and interaction of addition of AMF has significant effect of height of balsa. A single treatment of organic pot composition has significant effect of total dry weight and diameter increment of balsa. A single treatment by adding AMF has significant effectfor increment of diameter, total dry weight, and percentage of mycorrhiza colonizationat the root ofbalsa. Keyword: Arbuscular Mycorrhizal Fungi (AMF), Balsa (O. bicolor), organic pot
Effect of Iron Toxicity on the Growth of Calliandra calothyrsus and Leucaena leucocephala Seedlings Mohammad Agus Salim; Luluk Setyaningsih; Imam Wahyudi; Sri Wilarso Budi R
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 3 (2021): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.65654

Abstract

Iron (Fe) is a micro essential needed by plants in small amounts and can be toxic when available in large quantities. This study aimed to evaluate how Fe exposure affects the growth of C. callothyrsus and L. leucocephala seedlings. This study used a completely randomized design with factorial, where the first factor consisted of two levels of seedlings (C. calothyrsus and L. leucocephala), and the second factor consisted of Fe concentration which consisted of 8 levels (0, 0.25, 0.5, 0.75, 1, 1.25, 1.5, and 1.75 mM). The results showed that treatment of seedlings species and concentration of Fe was able to significantly affect the growth parameters (height, root length, root dry weight, shoots, and plant dry weight) of seedlings. The control treatment (without Fe) showed the highest growth response compared to those treated with Fe exposure and an increase in Fe concentration was able to reduce all growth parameters in both seedlings. The 0.5 mM Fe concentration reduced all growth parameters of C. calothyrsus drastically, while in L. leucocephala, the Fe 0.75 concentration was able to decrease all growth parameters drastically. The tolerance index of both seedlings decreased with increasing Fe concentration. The rate of photosynthesis did not show a significant difference between treatments, meanwhile, it had a significant effect on chlorophyll affect chlorophyll (a, b, and total chlorophyll) and carotenoid content. The highest Fe content in C. calothyrsus seedlings was at a concentration of 1.5 mM (4.40%), while in L. leucocephala seedlings, the highest Fe content was at 1.7 mM (2.87%). 
Isolation and Identification of Mycorrhizosphere Bacteria and Their Antagonistic Effects Towards Ganoderma boninense in vitro YENNI BAKHTIAR; SUDIRMAN YAHYA; WAHONO SUMARYONO; MEITY SURADJI SINAGA; SRI WILARSO BUDI; TEUKU TAJUDDIN
Microbiology Indonesia Vol. 4 No. 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8578.755 KB) | DOI: 10.5454/mi.4.2.9

Abstract

Basal stem rot caused by Ganoderma boninense is the most serious disease of oil palm (Elaeis guineensis) in Indonesia and it has caused major loss in palm oil production. Mycorrhizosphere bacteria offer possible advantages as biocontrol agents as they live and proliferate together with arbuscular mycorrhizal fungi, which have an ability to increase plant resistance against pathogens. A study was conducted to isolate mycorrhizosphere bacteria from spores of arbuscular mycorrhizal fungi and test their antagonistic effects against G. boninense in vitro. All bacterial isolates were identified based on 16S rDNA analysis and it revealed that eleven out of twenty mycorrhizosphere bacteria isolated were related to Bacillus with similarity ranging from 97 to 100%, whereas other isolates were identified as Pseudomonas, Streptomyces, Kocuria, Enterobacter, Brevundimonas, and Alcaligenes with similarities ranging from 96 to 100%. Fourteen out of twenty mycorrhizosphere bacteria showed a varying degree of inhibition towards the growth of G. boninense in vitro. Of these, isolate B10 (closely related to Bacillus subtilis ZJ06) showed the highest inhibitory effect followed by B17 (closely related to Bacillus subtilis N43). Therefore, these bacteria have a potential to be used as biocontrol agents to control basal stem rot disease caused by G. boninense in oil palm.
Bacteria from Arbuscular Mycorrhizal Fungi Spores Gigaspora sp. and Glomus sp. : Their Antagonistic Effects towards Soilborne Fungal Pathogens and Growth Stimulation of Gigaspora sp. in vitro Sri Wilarso Budi
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 20 No. 1 (2013)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2013.20.1.252

Abstract

Eight isolates bacteria were isolated from surface sterilized endomycorrhizal spores of Gigaspora sp and six isolates bacteria isolated from Glomus sp. The tests to  the antagonists against fungal pathogens and stimulation of endomycorrhizae hyphal development of Gigaspora sp  in vitro  showed that there were three.isolates (GG1, GG5 and GL3) that have the ability to inhibit the growth of pathogens and enhance the development of hyphae in vitro. Enzymatic activity test showed that there were seven isolates that produce cellulase and protease activity that is GG1, GG3, GG6, GG7, GL2, GL4 and GL6 Key words : Bacteria, Endomycorrhizal Spores, Antagonistic effects, Stimulation effects, Fungal pathogens
DNA BARCODE CHARACTERIZATION OF MISTLETOE INFESTATION IN TEAK CLONAL SEED ORCHARD (CSO) IN PADANGAN, EAST JAVA PROVINCE, INDONESIA Zainal Muttaqin; Sri Wilarso Budi; Basuki Wasis; Corryanti Corryanti; Iskandar Zulkarnaen Siregar
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 24 No. 2 (2017)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2755.603 KB) | DOI: 10.11598/btb.2017.24.2.651

Abstract

For effective teak plantation management, early detection system in controlling teak mistletoe requires various basic information, including degree of infestation and accuracy of the species names. Mistletoe infestations in teak and mistletoe species name have been reported, but there are still problems in identifying the correct species or subspecies due to morphological similarity.  The objective of this study was to clarify the species identity of hemi-parasitic mistletoe plants, which were found in teak Clonal Seed Orchard (CSO) in Padangan, East Java Province, Indonesia using DNA barcodes. Species identification of teak mistletoe based on leaf morphological characteristics and universal DNA barcode regions (i.e. matK and rbcL) were carried out. The results showed that the Canonical Discriminant Analysis (CDA) could differentiate Dendrophthoe pentandra and Macrosolen tetragonus based on leaf morphological characteristics. Variables having high correlation to distinguish both species were length of petiole,  width of the widest leaf, number of secondary leaf veins, leaf base shape, aspect ratio, form factor and perimeter ratio of diameter.  The results of  DNA barcoding showed that the two DNA barcode regions presented good  amplification and sequence results. Both DNA barcode regions successfully differentiated two species i.e. D. pentandra and M. tetragonus which belong to Loranthaceae family and have similar leaf morphological characteristics. Those regions were also able to identify Viscum articulatum and other species belonging to Santalaceae family. These results suggested that the two DNA regions could become recommended universal DNA barcode for identifying teak mistletoe.
JABON (Anthocephalus cadamba Roxb) POTENCY FOR REMEDIATING LEAD (Pb) TOXICITY UNDER NUTRIENT CULTURE CONDITION Luluk Setyaningsih; Yadi Setiadi; Sri Wilarso Budi; Hamim Hamim; Didy Sopandie
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 25 No. 1 (2018)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2018.25.1.712

Abstract

Information on metal adaptation of plants is necessary to understand the mechanism of heavy metal, including lead (Pb), remediation mediated by forest plants in contaminated land. This study aimed to find out the adaptation mechanism of jabon (Anthocephalus cadamba Roxb) seedlings to excessive lead level based on the tolerance index of growth performance and lead transport to plant tissue.  The seedlings were exposed to lead (Pb(NO3)2) with the concentrations of 0, 0.5, 1, 1.5, 5 and 10 mM in nutrient culture for 15 days. The result showed that the tolerance index (TI) of the seedlings was significantly decreased by Pb exposure up to 1.5 mM, but the TI values were more than 75%.  All seedlings died at Pb concentration of 5 mM and up.  Pb accumulated in all parts of the seedlings, with the highest concentration found in the leaves (735.9 ppm) under Pb concentration of 0.5 mM.   The Pb was found to be transported to the top portion of the seedlings indicated by Transport Factor (TF) that was more than 1. The results suggest that jabon can adapt to excessive Pb exposure up to 1.5 mM and has the potential as a remediator plant.
GENETIC VARIATION OF TEAK MISTLETOE (Dendrophthoe pentandra (L.) MIQ.) BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS Zainal Muttaqin; Sri Wilarso Budi; Basuki Wasis; Iskandar Zulkarnaen Siregar; Corryanti Corryanti
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 27 No. 2 (2020)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.519 KB) | DOI: 10.11598/btb.2020.27.2.1211

Abstract

Mistletoes are hemiparasitic macroparasite plants which interfere with trees and other wild plants in nutrient acquisition. As the plant has low leaf water potential, it draws water from teak wood tissues during the deciduous stage of the teak host, thereby killing the twigs and eventually, the teak tree. Mistletoes are also a key player in plant diversity. Therefore, the mistletoe population needs to be regulated not only as a parasite but also as a keystone species affecting biodiversity. Knowledge scarcity on the status of mistletoes includes its genetic variation. Hence, the purpose of this study is to analyze the level of genetic variation of teak mistletoe (Dendrophthoe pentandra) using RAPD marker. At Padangan teak Clonal Seed Orchard (CSO), it was randomly collected leaf samples from three layers of the mistletoe’s crown (upper, middle, and below) were taken from five host teak trees randomly selected from each of the sub-observation measure plots (OMP). Four OMP units inside the observation sample plots (OSP) (n = 3, 50 x 50 m) at different levels of infestation (light, moderate and heavy) were established. Analysis of the genetic variation and genetic distance of mistletoes hanging on the different crown layers were conducted using RAPD markers. The leaf samples from the crown layers, UU (upper crown and sub-section upper), UM (upper crown and sub-section middle), and UB (upper crown and sub-section below), which include U (upper crown) had significantly greater genetic variation (He = 0.181 – 0.255) than those from M layer (middle crown, He = 0.227) and the B layer (below crown, He = 0.114). Furthermore, the widest genetic distance significantly occurred between the mistletoes of the UB and B crown layers (0.310), whereas the nearest genetic distance significantly occurred between mistletoes of UU and UM layers in the upper crown (0.038). Practical implications of the low genetic variation in this study include the control of mistletoe D. pentandra infestation by means of restricting its population so that Perhutani State Owned Forestry Enterprise can maintain the level of damage below the economic threshold.
Coal Waste Powder Amendment and Arbuscular Mycorrhizal Fungi Enhance the Growth of Jabon (Anthocephalus cadamba Miq) Seedling in Ultisol Soil Medium Sri Wilarso Budi; Fiona Christina
JOURNAL OF TROPICAL SOILS Vol 18, No 1: January 2013
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2013.v18i1.59-66

Abstract

Coal powder waste application on low nutrient media is expected to be able to increase plant growth and to improve Arbuscular mycorrhizal fungi (AMF) development. The objective of this research was to determine the effect of coal waste powder on the growth of Anthocephallus cadamba Jack and AMF development grown on ultisol soil. Two factors in a completely randomised experimental design was conducted under greenhouse conditions and Duncan Multiple Range Test was used to analyse of the effect the treatment. The first factor was ultisol soil ammended with coal waste powder (control, soil amanded with coal waste 5%, soil amanded with coal waste 10% and soil amanded with coal waste 15%) and the second factor was AMF inoculation (uninoculated control, inoculated with Gigaspora margarita). Plant height, diameter, shoot dry weight, percentage of AMF colonization and nutrient uptake were measured in this experiment. Results of this study showed that coal amendment and AMF when applied separately significantly increased height, diameter, shoot dry weight, root dry weight and nutrient uptake of 12 weeks A. cadamba seedling, but when the coal waste powder and AMF were combined the plant growth parameters were lower than those applied separately but significantly higher than control. The application of coal waste powder or AMF in ultisol soil could increase A. cadamba growth and development.[How to Cite : Budi SW and F Christina. 2013. Coal Waste Powder Amendment and Arbuscular Mycorrhizal Fungi Enhance the Growth of Jabon (Anthocephalus cadamba Miq) Seedling in Ultisol Soil Medium. J Trop Soils, 18 (1): 59-66. doi: 10.5400/jts.2013.18.1.59][Permalink/DOI: www.dx.doi.org/10.5400/jts.2013.18.1.59]
Co-Authors , Hariyadi AAboe B. Saidi Abdurrani Muin Adisti Permatasari Putri Hartoyo Agustina Puspita Dewi Aji Nuralam Dwisutono Akhmad Wahyudi Alfi Laila Zuhriansah Allaily Allaily Andi Sukendro Ani Kurniawati Arif Budi Setiawan Arum Sekar Wulandari Atang Sutandi Bambang S. Purwoko Baskoro Rochaddi Basuki Wasis Bedah Rupaedah Berry Oktavianto Betty Purwati Cahyo Wibowo Ceng Asmarahman Corryanti . Corryanti Corryanti Corryanti Corryanti Dadan Mulyana Dida Syamsuwida Didah Nur Faridah Didy Sopandie DWI ANDREAS SANTOSA Dwi Puspasari Arum Ramadhani Endah Retno Palupi Endang Suhendang Eny Widajati Erdy Santoso Erdy Santoso Faisal Al Asad Fatimah Nur Istiqomah Fiona Christina Fransisca Yosina Regina Luturmas Gunawan Gunawan Hamim Hamim Husna Husna Husna Husna Hutami Indah Pertiwi Ikbal . Ikbal Ikbal Imam Wahyudi Indrawan, Imam Wahyudi Intan Fajar Kemala Irdika Mansur Iskandar . Iskandar Iskandar Iskandar M. Lapanjang Iskandar Z Siregar ISKANDAR ZULKARNAEN SIREGAR Istomo . Iswandi Anas JAYANI, FARADILA MEI Jumadil Akhir Kartika Megawati Kartini Kramadibrata Kartini Kramadibrata Khoryfatul Munawaroh Lina Karlinasari Luluk Setyaningsih Maman Turjaman Maman Turjaman Maman Turjaman Maman Turjaman Maman Turjaman Maryani Fatimah Putri Hardhani Maya Melati MEITY SURADJI SINAGA MEITY SURADJI SINAGA Mohammad Agus Salim Mohammad Agus Salim Salim Muhammad Helmi Muttaqin, Zainal Nelly Fridayanti Noor F Mardatin NUNANG LAMAEK MAY Nurheni Wijayanto Panca Dewi MH Karti Prijanto Pamoengkas Putri Aurum Putri Aurum Rahmawati Rahmawati Sabti Indah Purwanti Safinah S. Hakim S Hakim Safinah Surya Hakim Satriyas Ilyas Siti Hanna Ghaida Sri Muryati Sri Muryati Sri Muryati Sudirman Yahya SUDIRMAN YAHYA Tedi Yunanto Teuku Tajuddin Tirsa Eka Saputri Ulfah Juniarti Siregar Wahono Sumaryono Yadi Setiadi Yadi Setiadi Yan Eka Prasetyawati YENNI BAKHTIAR YENNI BAKHTIAR YENNI BAKHTIAR Zainal Muttaqin