Garuda - Garba Rujukan Digital

p-Index From 2019 - 2024

5.684

P-Index

This Author published in this journals

All Journal Medicina E-Jurnal Medika Udayana Jurnal Veteriner e-CliniC JBN (Jurnal Bedah Nasional) ISM (Intisari Sains Medis) : Jurnal Kedokteran Majalah Patologi Indonesia Jurnal Respirasi (JR) Neurologico Spinale Medico Chirurgico Jurnal Kedokteran Universitas Palangka Raya Indonesian Journal of Cancer Journal of Global Pharma Technology

Ni Putu Sriwidyani

Departemen Patologi Anatomi Fakultas Kedokteran Universitas Udayana

Author-ID : 386139

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Veterinary

Published : 49 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3 4 5

Loss of Phosphatase and Tensin Homologue (PTEN) Expression Associated with Higher Risk Grade Group Gleason Prostate Adenocarcinoma in Sanglah Hospital Denpasar Tjandra Kristiana; I Gusti Ayu Sri Mahendra Dewi; Luh Putu Iin Indrayani Maker; Herman Saputra; Ni Putu Sriwidyani; I Made Muliarta
Indonesian Journal of Cancer Vol 13, No 4 (2019): December
Publisher : National Cancer Center - Dharmais Cancer Hospital

Background: Prostate cancer is the second most common malignancy in men and the fifth most common cause of death worldwide. In Asia, 59.3% of patients come for the advanced stage treatment. PTEN inactivation is identified in about 20% of primary prostate tumors in radical prostatectomy and the loss of PTEN is associated with poor clinical and pathological outcomes. The purpose of this study is to prove that there is an association between PTEN expression and risk grade group Gleason prostate adenocarcinoma in Sanglah Public Hospital, Denpasar.Methods: This is a cross-sectional study. The sample size of this study was 35 paraffin blocks. These samples were selected by proportional stratified random sampling from hematoxylin-eosin preparation. Prostate adenocarcinoma was regrouped into 3 categories based on NCCN risk stratification: low risk grade group Gleason (Gleason score ≤ 6), intermediate risk grade group Gleason (Gleason score 7), and high-risk grade group Gleason (Gleason score 8–10). Immunohistochemistry examination of PTEN was performed and the expression was evaluated by scoring method. The data were analyzed by Chi-square and logistic regression.Results: The analysis result showed that there is an association between PTEN expression and risk grade group Gleason that is statistically significant. The loss of PTEN expression associated with higher risk grade group Gleason is of the higher proportion with p=0.001; PR 3.339; 95% CI: 1.296–8.599, but there is no association between the proportion loss of PTEN expression heterogeneously or homogeneously and the risk grade group Gleason with p=0.742; PR 0.663; 95% CI: 0.179–2.457.Conclusions: This study has proved that PTEN expression is associated with higher risk grade group Gleason prostate adenocarcinoma.

Tethered Cord Syndrome in Patient with Lipomyelomeningocele: A Case Study I Gusti Ayu Sri Mahendra Dewi; Ni Putu Sriwidyani; Desak Made Cittarasmi Saraswati Seputra
Indonesian Journal of Cancer Vol 16, No 2 (2022): June
Publisher : National Cancer Center - Dharmais Cancer Hospital

Introduction: Tethered Cord Syndrome (TCS) is a condition that occurs due to spinal cord traction caused by the attachment of the spinal cord to the surrounding tissues. The incidence of TCS is quite rare, approximately 0.25 per 1,000 live births Case Presentation: We reported a 4-year-old female patient with a lump on her sacral region since birth which enlarged over time. The patient experienced intermittent fever for 1 month before hospital admission and constipation since infancy. Physical examination revealed a lump with a diameter of 6 cm on her sacral region with no neurological abnormality. Ultrasonography examination showed an anechoic lesion with multiple linear structures on supragluteal sinistra with a size of 2.2 x 2.5 x 2.1 cm. MSCT findings showed spina bifida at L5 until S2 level with features of lipomyelomeningocele and tethered cord started from L2 level. Bilateral hydronephrosis, hydroureter, and distended bladder (pine tree appearance) with the possibility of the neurogenic bladder were also found on MSCT examination. The patient underwent surgery to excise the lump and to release the tethered cord. Microscopic findings from excised mass showed a group of neuronal and glial cells, the syncytial sheet pattern of meningothelial cells, and foci of calcification which supported lipomyelomeningocele diagnosis. Conclusions: : Intensive follow-up care and observation must be carried out in children with spinal dysraphism because it may be associated with TCS which may be asymptomatic at an early stage.

Benign Inflammatory Lesion Mimicking Malignancy “Kimura’s Disease”: A Case Report Putu Erika Paskarani; Luh Ayu Widayanti; Anak Agung Ayu Ngurah Susraini; Herman Saputra; Ni Putu Sriwidyani
Indonesian Journal of Cancer Vol 14, No 4 (2020): December
Publisher : National Cancer Center - Dharmais Cancer Hospital

Introduction: Kimura’s disease (KD) in Indonesia is quite rare. It is a distinct benign reactive process; however, it is usually mimicking malignancy. It usually affects young adults with a peak incidence in three decades, and men are mostly affected compared to women with a 3:1 ratio. KD is a chronic inflammatory disorder characterized by lymphoid hyperplasia, eosinophilia, and associated with soft tissue swelling that usually arises in head and neck regions with regional lymphadenopathy.Case Presentation: Herein, we report a case in a 55-year-old man from East Indonesia who presented a huge lump on his left head and neck regions. The lump started about 8 years ago. Physical examination found a tumor mass of 20 x 10 cm with indistinct border and multiple lymphadenopathies left coli with the biggest size of 1.3 x 0.7 x 0.5 cm. Peripheral blood examination shows marked eosinophilia. Nasopharyngeal CT scan revealed soft tissue masses and multiple lymphadenopathies suspected of malignancy; however, thorax plain radiology did not show any sign of metastatic process. Histopathological examination shows reactive follicular proliferation, extensive eosinophilia, focal area Folliculolysis, and polykaryocytes of the WarthinFinkeldey type.Conclusion: Based on a complete history, laboratory findings, and histopathological examination, this case is concluded as Kimura’s disease. Although clinical findings and radiological examination suspected it as malignancy, those discrepancies were confirmed as a benign lesion by specific histopathological and laboratory findings. Commonly, most patients have a favorable prognosis and good response to therapy.

Karakteristik klinikopatologi osteokondroma di Rumah Sakit Umum Pusat Sanglah Denpasar periode tahun 2013 - 2018 I Gusti Ngurah Pratama Yuda Atmaja; I Wayan Juli Sumadi; Ni Putu Sriwidyani
Intisari Sains Medis Vol. 11 No. 3 (2020): (Available online: 1 December 2020)
Publisher : DiscoverSys Inc.

Background: Osteochondroma is a benign cartilage tumor which is quite a number of events with a clinical picture and quite varied pathology. The cause of osteochondroma is still not known with certainty. Data from osteocondroma cases in Indonesia especially in Bali are still very rarely found, even histopathological data registered at hospitals in Bali are still very rare. Aim: The study aims to determine the clinicopathological characteristics of osteochondroma patients in the Sanglah Hospital Denpasar for 5 years.Method: This research is a cross-sectional descriptive study conducted at the Sanglah Hospital using the histological data archive for the period 2013 - 2018. The sampling technique used total sampling, with a total sample of 32 people.Results: Most of the osteochondroma sufferers in this study were 59.4% male, while female sufferers were around 40.6%, with the most age range being 10 - 20 years (62.5%) and at least those aged > 20 years (15.6%). The most tumor location is the distal femur bone (37.5%). Most tumor size is at the size of 1-5 cm (78.1%). Most cases of osteochondroma based on the thickness of the hyaline cartilage cap are 1-3 mm (59.4%).Conclusion: The clinicopathological characteristics of osteochondroma patients by using histopathological data in the Sanglah Hospital shows that osteochondroma is more common in men. Osteokondroma merupakan salah satu tumor tulang rawan jinak yang angka kejadiannya cukup banyak dengan gambaran klinis dan gambaran patologinya cukup bervariasi. Penyebab osteokondroma masih belum dapat diketahui dengan pasti. Data dari kasus osteokondroma di Indonesia khususnya di Bali masih sangat jarang ditemukan, bahkan data histopatologi yang teregristasi di rumah sakit di Bali masih sangat jarang terjadi. Tujuan: Untuk mengetahui karakteristik klinikopatologi pasien osteokondroma di Rumah Sakit Umum Pusat (RSUP) Sanglah Denpasar selama 5 tahun.Metode: Penelitian ini merupakan penelitian deskriptif cross-sectional yang dilaksanakan di RSUP Sanglah menggunakan arsip data histologi periode tahun 2013 – 2018. Teknik pengambilan sampel menggunakan total sampling, dengan total jumlah sampel sebanyak 32 orang.Hasil: Sebagian besar penderita osteokondroma pada penelitian ini adalah laki – laki selitar 59,4%, sedangkan penderita perempuan sekitar 40,6%, dengan rentang usia terbanyak adalah 10 – 20 tahun (62,5%) dan paling sedikit pada usia > 20 tahun (15,6%). Lokasi tumor paling banyak adalah tulang distal femur (37,5%). Ukuran tumor paling banyak adalah pada ukuran 1 – 5 cm (78,1%). Kasus osteokondroma berdasarkan ketebalan tutup tulang rawan hialin terbanyak adalah pada ukuran 1 – 3 mm (59,4%).Simpulan: Karakteristik klinikopatologi pasien osteokondroma dengan menggunakan data histopatologi di RSUP Sanglah menunjukkan osteokondroma lebih banyak diderita oleh laki – laki.

Hubungan antara Tumor Infiltrating Lymphocytes (TIL), ekspresi Programmed Death-Ligan 1 (PD-L1) pada sel tumor dan TIL dengan kedalaman invasi pada karsinoma urotelial kandung kemih tipe tidak spesifik di RSUP Sanglah, Bali, Indonesia Luh Ayu Widayanti; I Gusti Ayu Sri Mahendra Dewi; Herman Saputra; Anak Agung Ayu Ngurah Susraini; Ni Putu Sriwidyani; I Made Muliarta
Intisari Sains Medis Vol. 11 No. 3 (2020): (Available online: 1 December 2020)
Publisher : DiscoverSys Inc.

Background: Urotelial carcinoma is one of carcinoma with increasing morbidity and mortality. The depth of invasion is one of risk factors that influence recurrence and survival rate in urotelial carcinoma. Patient with deeper invasion has poorer prognosis in recurrence and survival rate. Generally, carcinoma have microenvironment such as tumor infiltrating lymphocytes (TIL) to eliminate the tumor cells, in proliferative area. Cancer cells can avoid host immunity through PD-1/PD-L1 pathway. This study aimed to determine the association between TIL and PD-L1 expression with the depth of invasion of urotelial carcinoma.Methods: This study was a cross sectional analytic study with 37 samples size. The samples were taken from paraffin block of patients with urotelial bladder carcinoma non-specific type, that has performed resection and histopathological examination at Anatomical Pathology Laboratory Sanglah Hospital Denpasar, from January 1st, 2015 to December 31th, 2019. Rediagnosis was carried out to assess the histopathological diagnosis, TIL and depth of invasion. Then PD-L1 immunohistochemistry was performed. Data were analyzed by Chi-Square test with p<0.05. Data were analyzed using SPSS version 20 for Windows.Results: In this study on bladder urothelial carcinoma non-specific type at Sanglah Hospital Denpasar, we found there is no association between TIL and the depth of invasion (p=0.471). There is association between PD-L1 expression in tumor cells with the depth of invasion (p=0.006), OR 7.04 (CI 95% =1.64-30.20). There is association between PD-L1 expression in TIL with the depth of invasion (p=0.001), OR 13.2 (CI 95% = 2.62-66.43).Conclusion: There is association between PD-L1 expression in tumor cells an PD-L1 expresion in TIL with the depth of invasion. Positive expression of PD-L1 in tumor cells and TIL are more likely to become advance invasion. Tumor Infiltrating Lymphocytes (TIL) and PD-L1 expression on TIL can be used as predictive factors for the depth of invasion in urotelial carcinoma. Latar Belakang: Karsinoma urotelial merupakan salah satu karsinoma dengan morbiditas dan mortalitas yang terus meningkat. Salah satu faktor risiko yang mempengaruhi rekurensi dan survival rate pada karsinoma urotelial adalah kedalaman invasi. Invasi yang lebih dalam memiliki prognosis yang lebih buruk. Karsinoma umumnya disertai lingkungan mikro antara lain tumor infiltrating lymphocytes (TIL) yang berfungsi untuk mengeliminasi tumor. Sel kanker memiliki kemampuan untuk menghindari imun host antara lain melalui ikatan PD-1 dengan PD-L1. Tujuan dari penelitian ini untuk mengetahui hubungan antara TIL dan ekspresi PD-L1 dengan kedalaman invasi pada karsinoma urotelial.Metode: Penelitian ini merupakan studi analitik potong lintang dengan besar sampel adalah 37, yang berasal dari blok parafin penderita karsinoma urotelial kandung kemih tipe tidak spesifik yang diperiksa histopatologi di Laboratorium Patologi Anatomi RSUP Sanglah Denpasar dari 1 Januari 2015 sampai 31 Desember 2019. Rediagnosis preparat dilakukan untuk menilai diagnosis histopatologi, TIL serta kedalaman invasi. Kemudian dilakukan pulasan imunohistokimia PD-L1. Data dianalisis dengan uji Chi-Square dengan nilai p<0,05. Data dianalisis dengan SPSS versi 20 untuk Windows.Hasil: Hasil penelitian ini menunjukkan bahwa pada karsinoma urotelial kandung kemih tipe tidak spesifik di RSUP Sanglah Denpasar, tidak terdapat hubungan yang signifikan antara TIL dengan kedalaman invasi (p=0,471). Terdapat hubungan yang signifikan antara ekspresi PD-L1 pada sel tumor dengan kedalaman invasi (p=0,006) dengan OR 7,04 (IK 95% =1,64-30,20), antara ekspresi PD-L1 pada TIL dengan kedalaman invasi (p=0,001) dengan OR 13,20 (IK 95% = 2,62-66,43).Kesimpulan: Terdapat hubungan antara ekspresi PD-L1 pada sel tumor dan pada TIL dengan kedalaman invasi pada karsinoma urotelial kandung kemih tipe tidak spesifik di RSUP Sanglah Denpasar. Ekspresi positif PD-L1 pada sel tumor dan pada TIL memiliki kemungkinan lebih besar untuk menjadi invasi lanjut.

Hubungan antara Stromal Tumor Infiltrating Lymphocytes (TIL) dengan ekspresi Programmed Death-Ligand 1 (PD-L1) pada karsinoma payudara invasif tipe tidak spesifik subtipe Human Epidermal Growth Factor Receptor-2 (HER-2) enriched di RSUP Sanglah, Bali, Ind Popi Imelda Margareth Sitompul; Herman Saputra; Luh Putu Iin Indrayani Maker; I Gusti Ayu Sri Mahendra Dewi; Ni Putu Sriwidyani; I Made Muliarta
Intisari Sains Medis Vol. 12 No. 2 (2021): (Available Online: 1 August 2021)
Publisher : DiscoverSys Inc.

Background: Breast cancer is the most common malignancy cancer in women. This malignancy is molecularly heterogeneous, where overexpression of the HER-2 growth factor receptor generally has a more aggressive nature. The role of immune system interaction, namely Tumor Infiltrating Lymphocytes (TIL) and Programmed Death-Ligand 1 (PD-L1) expression, is associated with prognostic and predictive factors. Therefore, this study aims to investigate the association between stromal TIL with PD-L1 expression in invasive breast carcinoma non-specific type HER-2 enriched subtype.Methods: This study was an observational analytical study with cross-sectional design from January 1st 2015 to December 31st 2019. The samples were taken from a paraffin block of patients with invasive breast carcinoma non-specific type, that has performed histopathological examination, ER, PR, HER-2 and FISH/CISH immunohistochemistry examination at the Anatomical Pathology Laboratory of Sanglah Hospital Denpasar. The samples obtained were stained with PD-L1 immunohistochemistry. The preparation was evaluated to assess TIL. Data were analyzed using SPSS version 20 for Windows.Results: The study found a statistically significant association between stromal TIL with PD-L1 expression in tumor cells (p = 0.018). There is statistically significant association between stromal TIL with PD-L1 expression in TIL (p = 0.001). There is statistically significant association between stromal TIL with PD-L1 expression in total tumor cells and TIL (p = 0.007) in invasive breast carcinoma non-specific type HER-2 enriched subtype at Sanglah Hospital, Denpasar.Conclusion: In conclusion, there is an association between stromal TIL with the expression of PD-L1 in tumor cells and TIL in invasive breast carcinoma non-specific type HER-2 enriched subtype. Latar Belakang: Kanker payudara merupakan keganasan tersering pada wanita. Keganasan ini bersifat heterogen ditinjau dari aspek molekuler, dimana overekspresi HER-2 umumnya memiliki sifat yang lebih agresif. Peranan sistem imun, yakni ekspresi Tumor Infiltrating Lymphocytes (TIL) dan Programmed Death-Ligand 1 (PDL-1) dihubungkan dengan faktor prognostik. Oleh karena itu, penelitian ini bertujuan untuk mengevaluasi hubungan antara stromal TIL dengan ekspresi PD-L1 pada karsinoma payudara invasif tipe tidak spesifik subtipe HER-2 enriched.Metode: Penelitian ini merupakan penelitian observasional analitik dengan rancangan studi potong lintang mulai dari 1 Januari 2015 sampai 31 Desember 2019. Sampel adalah blok parafin penderita karsinoma payudara invasif tipe tidak spesifik yang telah dilakukan pemeriksaan histopatologi, imunohistokimia ER, PR, HER-2 dan FISH/CISH di Laboratorium Patologi Anatomi RSUP Sanglah Denpasar. Sampel yang diperoleh dilakukan pulasan imunohistokimia PD-L1 Preparat sampel digunakan untuk menilai TIL. Data dianalisis dengan SPSS versi 20 untuk Windows.Hasil: Pada penelitian ditemukan hubungan yang signifikan secara statistik antara stromal TIL dengan ekspresi PD-L1 pada sel tumor (p=0,018). Terdapat hubungan yang signifikan secara statistik antara stromal TIL dengan ekspresi PD-L1 pada TIL (p=0,001). Terdapat hubungan yang signifikan secara statistik antara stromal TIL dengan ekspresi PD-L1 pada total sel tumor dan TIL (p=0,007) pada karsinoma payudara invasif tipe tidak spesifik subtipe HER-2 enriched di RSUP Sanglah Denpasar.Kesimpulan: Hasil penelitian ini menunjukkan terdapat hubungan bermakna antara stromal TIL dengan ekspresi PD-L1 sel tumor dan TIL pada karsinoma payudara invasif tipe tidak spesifik subtipe HER-2 enriched.

Perbedaan ekspresi Vascular Endothelial Growth Factor (VEGF) pada meningioma risiko rendah dan risiko tinggi di RSUP Sanglah, Bali, Indonesia Ivana Juliarty Sitanggang; Ni Putu Sriwidyani; I Wayan Juli Sumadi; I Gusti Ayu Sri Mahendra Dewi; Luh Putu Iin Indrayani Maker; I Made Muliarta
Intisari Sains Medis Vol. 12 No. 2 (2021): (Available Online: 1 August 2021)
Publisher : DiscoverSys Inc.

Background: Vascular Endothelial Growth Factor (VEGF) is an angiogenic factor that plays an important role in tumor angiogenesis. VEGF in meningioma is up-regulated and indicates its role as a proangiogenic factor. It has an association with tumor recurrence and progression. This study aims to determine the differences in VEGF expression in low-risk and high-risk meningiomas at Sanglah General Hospital, Bali, Indonesia.Methods: This study was a cross-sectional analytical study with a sample size of 52, which came from meningioma patients examined histopathologically at the Anatomical Pathology Laboratory of Sanglah General Hospital from January to December 2019. The VEGF immunohistochemical staining was performed and interpreted using Histo score (H-score). VEGF expression was categorized into high and low expression, with the cut-off value determined based on the median value. Data were analyzed using SPSS version 20 for Windows.Methods: The results showed that out of 52 meningioma samples, 37 (71.1%) cases of low-risk meningiomas with low VEGF expression, 6 cases (11.5%) of low-risk meningiomas with high VEGF expression, and 9 cases (17.3%) of high-risk meningiomas with high VEGF expression. There was no high-risk meningioma with low VEGF expression. There was a significant difference in VEGF expression between the low-risk and high-risk meningioma groups (p = 0.00), and high VEGF expression had a prevalence risk for the incidence of high-risk meningioma by 2.5 times (95% CI=1.3-4.6).Conclusion: Based on the results of this study, it was concluded that there was a VEGF expression difference between low-risk and high-risk meningiomas in Sanglah General Hospital Denpasar and high VEGF had a prevalence risk for the occurrence of high-risk meningiomas by 2.5 times. Latar Belakang: Vascular Endothelial Growth Factor (VEGF) merupakan faktor angiogenik yang berperan penting dalam angiogenesis tumor. VEGF pada meningioma mengalami up-regulation yang menunjukkan perannya sebagai faktor proangiogenik yang berkaitan dengan rekurensi dan perkembangan tumor. Penelitian ini bertujuan untuk menentukan perbedaan ekspresi VEGF pada meningioma risiko rendah dan risiko tinggi di RSUP Sanglah, Bali, Indonesia.Metode: Penelitian ini merupakan studi analitik potong lintang dengan besar sampel adalah 52, yang berasal dari blok parafin penderita meningioma yang diperiksa histopatologi di Laboratorium Patologi Anatomi RSUP Sanglah Denpasar dari Januari - Desember 2019. Dilakukan pulasan imunohistokimia VEGF dan penilaiannya menggunakan Histo score (H-score). Ekspresi VEGF dikategorikan menjadi tinggi dan rendah dengan nilai cut-off yang ditentukan berdasarkan nilai median. Data dianalisis dengan SPSS versi 20 untuk Windows.Hasil: Hasil penelitian ini menunjukkan bahwa dari 52 sampel meningioma, terdapat 37 kasus (71,1%) meningioma risiko rendah dengan ekspresi VEGF rendah, 6 kasus (11,5%) meningioma risiko rendah dengan ekspresi VEGF yang tinggi, dan 9 kasus (17,3%) meningioma risiko tinggi dengan ekspresi VEGF tinggi. Tidak didapatkan meningioma risiko rendah dengan ekspresi VEGF rendah. Didapatkan perbedaan ekspresi VEGF yang bermakna antara kelompok meningioma risiko rendah dan risiko tinggi (p=0,00) dan ekspresi VEGF tinggi mempunyai risiko prevalensi untuk terjadinya meningioma risiko tinggi sebesar 2,5 kali (95% IK=1,3-4,6).Kesimpulan: Berdasarkan hasil penelitian ini didapatkan perbedaan ekspresi VEGF antara meningioma risiko rendah dan risiko tinggi di RSUP Sanglah Denpasar dan pada VEGF tinggi mempunyai risiko prevalensi untuk terjadinya meningioma risiko tinggi sebesar 2,5 kali.

Hubungan Lymph Vessel Density (LVD) dengan invasi limfatik dan metastasis kelenjar getah bening regional pada karsinoma payudara invasif tipe tidak spesifik di RSUP Sanglah, Bali, Indonesia Katrin Rotua Simbolon; I Gusti Ayu Sri Mahendra Dewi; Ni Putu Sriwidyani; Luh Putu Iin Indrayani Maker; Herman Saputra; I Made Muliarta
Intisari Sains Medis Vol. 12 No. 2 (2021): (Available Online: 1 August 2021)
Publisher : DiscoverSys Inc.

Background: Lymph Node Metastasis (LNM) is considered the most important prognostic factor in breast cancer. The progress of lymphatic metastasis is thought to involve the proliferation of lymphatic vessels (lymphangiogenesis), Lymph Vessel Invasion (LVI), and lymph node metastasis step by step. This study aims to evaluate the association between LVD with LVI and regional LNM in invasive breast carcinoma of no special type.Methods: This study used a cross-sectional analytical study, using a sample of 38 paraffin-embedded tissue from the patient with invasive breast carcinoma of no special type in Sanglah General Hospital, Bali, Indonesia, in 2018. The sample will be used to assess LVD and LVI by D2-40 immunohistochemistry. Expression of D2-40 with vigorous-intensity was evaluated on the membrane and cytoplasm of lymphatic endothelial cells to determine the definition of lymphatic vessels. Lymph vessel density was determined by counting the number of lumens of peritumoral lymphatic vessels in five fields of view with the densest lymphatic vessel lumen using a Leica microscope (DM750, 400x, area 0.225mm2). Data were analyzed using SPSS version 24 for Windows.Results: The LVD cut-off value is 16.5 lymphatic vessel lumens/0.225 mm2 (65.4% for sensitivity and area Under the Curve (AUC)=70.2%). Then cases were classified into the category of high LVD (?16,5 lymphatic vessel lumen/0,225 mm2) and low LVD (<16.5 lymphatic vessels lumen/0,225 mm2). The study found a statistically significant association between LVD and lymph vessel invasion (PR=3.6; p=0.043; 95%CI=0.88-14.78) and regional lymph node metastases (PR =1.7; p=0.02; 95%CI=1.03-2.79).Conclusion: This study proved an association between LVD with lymph vessel invasion and regional lymph node metastases. Latar Belakang: Metastasis Kelenjar Getah Bening (KGB) dianggap sebagai faktor prognostik yang paling penting pada kanker payudara. Perkembangan metastasis limfatik diduga melibatkan proliferasi pembuluh limfatik (limfangiogenesis), Lymph Vessel Invasion (LVI) atau invasi limfatik, dan metastasis kelenjar getah bening secara bertahap. Penelitian ini bertujuan untuk mengevaluasi hubungan antara Lymph Vessel Density (LVD) dengan invasi limfatik dan metastasis kelenjar getah bening regional pada karsinoma payudara invasif tipe tidak spesifik.Metode: Penelitian ini menggunakan metode analitik potong lintang dengan sampel 38 blok parafin dari pasien karsinoma payudara invasif tipe tidak spesifik di RSUP Sanglah, Bali, Indonesia tahun 2018. Penilaian LVD dan LVI dengan pulasan imunohistokimia D2-40. Ekspresi D2-40 dengan intensitas yang kuat dievaluasi pada membran dan sitoplasma sel endotel limfatik untuk menetapkan definisi pembuluh limfatik. Lymph vessel density ditentukan dengan menghitung jumlah lumen pembuluh limfatik peritumoral pada lima lapangan pandang yang memiliki lumen pembuluh limfatik terpadat, menggunakan mikroskop Leica (DM750, 400x, luas area 0,225mm2). Data dianalisis dengan SPSS versi 24 untuk Windows.Hasil: Nilai cut-off LVD adalah 16,5 lumen pembuluh limfatik/0,225 mm2 (sensitivitas 65,4% dan Area Under the Curve (AUC)=70,2%). Kemudian kasus dikategorikan menjadi LVD tinggi (?16,5 lumen pembuluh limfatik / 0,225 mm2) dan LVD rendah (<16,5 lumen pembuluh limfatik / 0,225 mm2). Hasil analisis statistik menunjukkan bahwa terdapat hubungan antara LVD dengan invasi limfatik (PR=3,6; p=0,043; IK 95%=0,88-14,78) dan metastasis KGB regional (PR=1,7; p=0,02; IK 95%=1,03-2,79).Kesimpulan: Penelitian ini membuktikan bahwa terdapat hubungan antara LVD dengan invasi limfatik dan metastasis KGB regional.

Hubungan antara ekspresi BRAF V600E dengan metastasis dan derajat diferensiasi pada adenokarsinoma kolorektal Ni Kadek Ayu Maya Damayanti; Ni Wayan Winarti; Ni Putu Sriwidyani; Luh Putu Iin Indrayani Maker; Herman Saputra; I Made Muliarta
Intisari Sains Medis Vol. 12 No. 2 (2021): (Available Online: 1 August 2021)
Publisher : DiscoverSys Inc.

Background: Colorectal carcinoma is a malignant epithelial tumor of the large bowel that occurs due to extrinsic and intrinsic factors. BRAF V600E gene mutation was found in about 10-15% of colorectal carcinoma. This mutation was associated with aggressive biologic behaviors, metastasis and lesser responsiveness to EGFR inhibitors therapy. This study aims to determine the association between BRAF V600E expression with metastasis and the degree of differentiation of colorectal adenocarcinoma.Methods: This cross sectional study involved 43 samples of adenocarcinoma colorectal patients who had histopathological examinations in the period 2018-2019. Immunohistochemical were performed to assess the expression of BRAF V600E. This expression was concluded positively if 75% or more tumor cells showed intense cytoplasmic staining. Data were analyzed using SPSS version 20 for Windows.Results: Within 43 colorectal adenocarcinoma cases, it was found 7 cases (16.3%) had a positive expression of BRAF V600E. This expression was found in metastasis cases (7/25), not found in cases without metastasis (0/18). There was an association between BRAF V600E expression and metastasis (p=0.014). The positive expressions of BRAF V600E were only found in low-grade differentiation (7/35 cases), were not found in high-grade differentiation (0/8 cases), and no association between BRAF V600E mutation and the degree of differentiation (p=0.167).Conclusion: There was an association between BRAF V600E expression and metastasis of colorectal adenocarcinoma. There was no significant association between BRAF V600E expression and the degree of differentiation of colorectal adenocarcinoma. Latar Belakang: Karsinoma kolorektal merupakan suatu tumor ganas epitelial usus besar yang terjadi akibat pengaruh dari faktor ekstrinsik dan intrinsik. Mutasi gen BRAF V600E ditemukan pada sekitar 10-15% kasus karsinoma kolorektal. Mutasi ini berkaitan dengan perilaku biologik agresif, metastasis, serta kurangnya respon terhadap terapi dengan inhibitor EGFR. Penelitian ini bertujuan untuk mengetahui adanya hubungan antara ekspresi BRAF V600E dengan metastasis dan derajat diferensiasi adenokarsinoma kolorektal.Metode: Penelitian menggunakan rancangan analitik potong lintang dengan 43 sampel penderita adenokarsinoma kolorektal yang telah dilakukan pemeriksaan histopatologi pada periode tahun 2018-2019. Pulasan imunohistokimia dikerjakan untuk menilai ekspresi BRAF V600E. Ekspresi BRAF V600E dinyatakan positif jika 75% atau lebih sel tumor mununjukkan pulasan sitoplasma dengan intensitas kuat. Data dianalisis dengan SPSS versi 20 untuk Windows.Hasil: Dari 43 kasus, ditemukan 7 kasus (16,3%) dengan ekspresi BRAF V600E positif. Ekspresi ini hanya dijumpai pada kasus dengan metastasis (7 dari 25 kasus), tidak dijumpai pada kasus tanpa metastasis (0 dari 18 kasus). Didapatkan hubungan bermakna antara ekspresi BRAF V600E dengan metastasis (p=0,014). Ekspresi BRAF V600E positif hanya ditemukan pada kasus derajat diferensiasi rendah (7 dari 35 kasus), tidak ditemukan pada derajat tinggi (0 dari 8 kasus), dan hubungan tersebut tidak bermakna secara statistik (p=0,167).Kesimpulan: Terdapat hubungan antara ekspresi BRAF V600E dengan metastasis pada adenokarsinoma kolorektal. Tidak terdapat hubungan antara ekspresi BRAF V600E dengan derajat diferensiasi pada adenokarsinoma kolorektal.

Hubungan antara status tumor budding dengan berbagai parameter klinikopatologi pada adenokarsinoma kolorektal di RSUP Sanglah, Bali, Indonesia Luh Putu Iin Indrayani Maker; Ni Putu Sriwidyani
Intisari Sains Medis Vol. 12 No. 3 (2021): (Available online: 1 December 2021)
Publisher : DiscoverSys Inc.

Background: Colorectal malignancies are common, with adenocarcinoma as the most prevalent type. Recently, studies of specific morphology like tumor budding have already started and related with tumor’s aggressivity as well as several parameters. This study aims to evaluate the association between tumor budding grade with patient age, sex, tumor location, histological grade, depth of invasion, and nodal status in colorectal adenocarcinoma at Sanglah General Hospital.Methods: This cross-sectional descriptive study used samples of intestinal resection specimens accompanied by Regional Lymph Node (RLN) removal of colorectal adenocarcinoma patients at Sanglah Hospital in 2018-2020. The sampling technique was carried out consecutively, which met the inclusion and exclusion criteria. Data on age, sex, tumor location, histologic grade, depth of invasion, and nodal status were obtained from the archives of examination results. At the same time, tumor budding data were obtained from observations of histopathological preparations with Hematoxylin-Eosin (H&E) staining. The results of the observations were tested statistically and presented descriptively. Data were analyzed with Chi-Square using SPSS version 22 for Windows.Results: Most of the study samples were 50 years old (84.0%), followed by male gender (62.0%), tumor location in the left colon (67.0%), low-grade (90.0%), T3-T4 invasion depth (77.0%), negative nodal status (60.0%), and high-tumor budding grade (51.0%). The Chi-Square test showed a significant relationship between the depth of invasion (p=0.001) and nodal status (p=0.009) variables on tumor budding grade.Conclusion: There were significant correlations between tumor budding grade and depth on invasion and nodal status. Latar Belakang: Kanker kolorektal merupakan keganasan yang sering ditemukan dengan adenokarsinoma merupakan tipe histopatologi terbanyak. Dewasa ini telah mulai diteliti morfologi spesifik yaitu tumor budding yang dikaitkan dengan agresivitas tumor maupun parameter lainnya. Penelitian ini bertujuan untuk mengetahui hubungan antara tumor budding grade dengan umur pasien, jenis kelamin, lokasi tumor, grade histopatologi, kedalaman invasi, dan status nodal pada adenokarsinoma kolorektal di RSUP Sanglah Denpasar.Metode: Penelitian deskriptif potong lintang ini menggunakan sampel spesimen reseksi usus disertai pengangkatan Kelenjar Getah Bening (KGB) regional pasien adenokarsinoma kolorektal di RSUP Sanglah tahun 2018-2020. Teknik pengambilan sampel dilakukan secara konsekutif, yang memenuhi kriteria inklusi dan eksklusi. Data umur, jenis kelamin, lokasi tumor, grade histopatologi, kedalaman invasi dan status nodal diperoleh dari arsip hasil pemeriksaan. Sedangkan data tumor budding diperoleh dari pengamatan sediaan histopatologi pewarnaan Hematoksilin-Eosin (H&E). Hasil pengamatan diuji secara statistik dan ditampilkan secara deskriptif. Data dianalisis dengan Chi-Square menggunakan SPSS versi 22 untuk Windows.Hasil: Sebagian besar sampel penelitian berusia ? 50 tahun (84,0%), diikuti dengan jenis kelamin laki-laki (62,0%), lokasi tumor pada kolon kiri (67,0%), low-grade (90,0%), kedalaman invasi T3-T4 (77,0%), status nodal negatif (60,0%), dan high-tumor budding grade (51,0%). Uji Chi-Square menunjukkan bahwa terdapat hubungan yang bermakna antara variabel kedalaman invasi (p=0,001) dan status nodal (p=0,009) terhadap tumor budding grade.Kesimpulan: Terdapat hubungan yang bermakna antara tumor budding grade dengan kedalaman invasi dan status nodal adenokarsinoma kolorektal.

Co-Authors Anak Agung Ayu Ngurah Susraini Angelia Ongko Prabowo annie minerva datui Bintang Dwi Oktaviani Desak Made Cittarasmi Saraswati Seputra Herlina Eka Shinta Herman Saputra Herman Saputra Herman Saputra Herman Saputra Herman Saputra Herman Saputra I Dewa Ayu Inten Primayanti I Gde Raka Widiana I Gede Budhi Setiawan I Gede Indradika Pratama Putra I Gusti Ayu Dewi Ratnayanti I Gusti Ayu Sri Mahendra Dewi I Gusti Kamasan I Gusti Kamasan Arijana I Gusti Ngurah Made Cesar Vajrashrava Sunantara I Gusti Ngurah Mayun I Gusti Ngurah Pratama Yuda Atmaja I Gusti Nyoman Sri Wiryawan I Kadek Yana Parastuta I Ketut Bawantika Adi Putra I Made Gotra I Made Muliarta I MADE MULIARTA . I Wayan Juli Sumadi I Wayan Sugiritama Ida Ayu Ista Nariswari Ida Ayu Jasminarti Dwi Kusumawardani Ida Ayu Jelantik Astuti Ida Ayu Krisna Cantika Dewi Ida Ayu Meilasari Dewi Ida Bagus Ardya Kurnia Wilananda Ivana Juliarty Sitanggang Jovi Carina Handoko Kadek Cindy Dwi Cahyani Kadek Denik Suastini Kadek Dwi Pradnyawati Kandy Rosa Ismalia Katrin Rotua Simbolon Ketut Mulyadi Komang Chandra Surya Dika Luh Ayu Widayanti Luh Ayu Widayanti Luh Komang Ayu Widhiaty Karang Luh Putu Iin Indrayani Maker Luh Yeni Laksmini M. Arif Perdana Ariyansyah Made Agus Cahya Nugraha Koriawan Moestikaningsih . Ni Gusti Ayu Agung Manik Yuniawaty Wetan Ni Kadek Ayu Maya Damayanti Ni Kadek Ayu Maya Damayanti Ni Made Mahastuti Ni Putu Ekawati Ni Wayan Candrawati Ni Wayan Winari Ni Wayan Winarti Nornazirah Binti Azizan Popi Imelda Margareth Sitompul Putra, Putu Krishna B. S. Putu Ayu Widya Pramesti Putu Erika Paskarani Putu Febry Krisna Pertiwi Putu Julia Chandra Devi Sang Ayu Putu Yuliantini Silvia Nuhyil Indriani Sumadi, I Wayan J. Sylvi Diahningrum Tjandra Kristiana Volman Tampubolon Wimpie I Pangkahila

Title

Found 49 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 49 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 49 Documents
Search