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All Journal Jurnal Sains dan Inovasi Perikanan/Journal of Fishery and Innovation (JSIPi) Jurnal Sosial Ekonomi Perikanan Panrita Abdi - Jurnal Pengabdian pada Masyarakat Jurnal Biologi Tropis Jurnal Pengabdian Magister Pendidikan IPA Jurnal Sains Teknologi Akuakultur Media Akuatika: Jurnal Ilmiah Jurusan Budidaya Perairan JURNAL SAINS dan INOVASI PERIKANAN Jurnal Sosial Ekonomi Perikanan
Muhammad Idris
Program Studi Ilmu Perikanan, Pasca Sarjana Universitas Halu Oleo, Kendari, Sulawesi Tenggara, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Other

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Journal : Jurnal Sains Teknologi Akuakultur

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Uji Daya Hambat Ekstrak Daun Mangrove (Sonneratia alba) pada Bakteri Vibrio harveyi secara In Vitro Kurniaji, Ardana; Idris, Muhammad; muliani, muliani
Jurnal Masyarakat Akuakultur Indonesia Vol 3, No 2 (2019): Volume 3 (2) 2020
Publisher : Masyarakat Akuakultur Indonesia

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Abstract

Abstrack In production effort of tiger shrimp (Pennaeus monodon) it had been found several problems that often faced of shrimp farmers in shrimp culture. One of them is the presence of vibriosis disease caused by Vibrio harveyi infection. This study aimed to examine the use of mangrove (S. alba) leaf extract by in vitro to prevent the population growth of bacteria V. harveyi. This research was conducted in the Laboratory of Pathology at Maros Research Institute for Brackish Water Aquaculture. Testing the inhibition of mangrove leaf extract to against bacterial has done in two phases namely qualitative test (inhibition zone) that used concentration were 2.000, 4.000, 6.000, 8.000, 10.000 ppm, control (without extract) and 25 ppm of antibiotic rifampicin as negative control. It the same time that quantitative test (bacterial population) that used concentration were 2.000, 4.000, 6.000, 8.000 ppm, control without extract and DMSO 10%. The results had showed mangrove leaf extract could inhibit the population growth of bacteria V. harveyi. In quality, extract categorized medium at concentration of 8,000-10,000 ppm with inhibition zone from 16.18 to 18.20 mm, and categorized low at 2000-6000 ppm concentration with inhibition zone 9.83 to 12.30 mm. While the quantity of best concentration of mangrove leaf extract in inhibiting the growth of bacteria were 8,000 ppm with total of bacteria was 0 CFU/mL after 8 hours, 6,000 ppm with total of bacteria was 0 CFU/mL after 12 hours, 4.000 ppm with total of bacteria was 4.80 x104 CFU/mL after 24 hours and 2.000 ppm with total of bacteria was 5.17 x107 CFU/mL after 24 hours.                                                                     Abstrak Dalam usaha produksi budidaya udang windu (Pennaeus monodon) ditemukan berbagai permasalahan yang sering menghambat petani dalam melaksanakan kegiatan budidaya di tambak. Salah satu diantaranya adalah adanya serangan penyakit vibriosis yang disebabkan oleh infeksi bakteri Vibrio harveyi. Penelitian ini bertujuan untuk menguji penggunaan ekstrak daun mangrove (S. alba) secara   In Vitro untuk mencegah pertumbuhan bakteri V. harveyi. Penelitian ini dilaksanakan di Laboratorium Patologi Balai Penelitian dan Pengembangan Budidaya Air Payau, Maros. Pengujian daya hambat ekstrak daun mangrove terhadap bakteri dilakukan dengan dua tahapan yakni secara kualitatif (zona hambatan) yang menggunakan konsentrasi 2.000, 4.000, 6.000, 8.000, 10.000 ppm, kontrol (tanpa pemberian ekstrak) dan antibiotik rifampisin 25 ppm serta pengujian secara kuantitatif (kepadatan bakteri) yang menggunakan konsentrasi 2.000, 4.000, 6.000, 8.000 ppm, kontrol dengan tanpa ekstrak dan DMSO 10%. Hasil penelitian menunjukkan ekstrak daun mangrove dapat menghambat pertumbuhan bakteri V. harveyi. Secara kualitas, ekstrak terkategori sedang pada konsentrasi 8.000-10.000 ppm dengan zona hambatan 16,18-18,20 mm,  dan terkategori lemah pada konsentrasi 2.000- 6.000 ppm  dengan zona hambatan 9,83-12,30 mm. Sedangkan secara kuantitas konsentrasi terbaik ekstrak daun mangrove berturut-turut dalam menghambat pertumbuhan bakteri adalah 8.000 ppm dengan total bakteri 0 CFU/mL setelah 8 jam, 6.000 ppm dengan total bakteri 0 CFU/mL setelah 12 jam, 4.000 ppm dengan total bakteri 4,80x104 CFU/mL setelah 24 jam dan 2.000 ppm dengan total bakteri 5,17x107 CFU/mL  setelah 24 jam. 
Co-Authors . Yusnaini, . ., Armin Abdul Majid Abdul Rahman Agus Kurnia Agus Kurnia Agus Kurnia Agus Kurnia AGUS KURNIA Agus Kurnia Agus Kurnia Agus Kurnia Ahmad Saltin Akbar, La Ode F. Akhril, Muhammad Amirullah Amirullah Amirullah Baharuddin Ardana Kurniaji Aslan, La Ode Muhammad Aslan, Laode M. Asnawai, . Baalu, Nurfati Balubi, Abdul M. Balubi, Abdul Muis Budiyanto Darman, Darman Effendy, Irwan J. Emiyarti Emiyarti Firman, . Fitriah, Asnin Friska Pattricya Noya Hamzan Wadi Hasni Hasni Husni Dilla Astari Iba, Wa Iba, Wa Indrawan, Muhammad A. Indriana, Nova Indriyani Nur Indriyani Nur Indriyani, Nur Ira Ira Irdam Riani Irdam Riani, Irdam Irwan Junaidi Effendy Jumaisa, . Jumarlin, Jumarlin Jumriati, Jumriati Kusnawan, Idham La Ode Baytul Abidin La Suriadi Lawelle, Sjamsu Alam Ma'ruf Kasim Mahmudin, Yuyun Masirudin, La Ode Muh. Nuryadin Muhaimin Hamzah Muhaimin Hamzah Muhaimin Hamzah Muhammad Ikhsan Muhammad Ramli Muhammad Ramli Muliani Muliani, Muliani Musair, Irdayanti Nahrullah, . Naimrudin, . Nining Putriyani Nur Asia, Nur Nur, Indriyani Oce Astuti Oce Astuti Pangerang, Utama K. Patadjai, Rahmad S. Piliana, Wa Ode Poluruy, Suparmin Rani Rani Rasak Mulki Rezki Amalyah Ruslaini Ruslaini, Ruslaini Safitri, Waode Saleh, Muhammad Y. Salim, Muhammad A. Sansibar, Sansibar Sari, Ade Irma Sarimudin, Rizal Sarini Yusuf Sitti, Sekar Aryati Sukriawan, Sukriawan Suparta, Syahruddin Utama Kurnia Pangerang Wa Ode Mutia Indah Wellem H. Muskita Yusnaini Yusnaini Yusnaini Yusnaini Yusnaini Yusriadi, Andi Zainuddin Saenong, Zainuddin