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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
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Articles 11 Documents
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Search results for , issue "Vol 12, No 2 (2011)" : 11 Documents clear
Respons Peristalsis dan Neuron Mienterik Nitrergik Usus Halus Kelinci yang Diinfeksi Eimeria magna Amelia Hana; Soesanto Mangkoewidjojo; Siti Isrina Oktavia Salasia2,; Dwi Liliek Kusindarta3
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Penelitian ini bertujuan untuk mengetahui respon usus kelinci yang diinfeksi Eimeria magna denganmengamati frekuensi dan amplitudo kontraksi peristalsis, dan jumlah neuron mienterik nitrergik usushalus. Hewan percobaan yang digunakan adalah 60 ekor kelinci jantan lokal sehat umur 5 bulan dengankisaran berat badan 1,5-1,8 kg, dan bebas koksidiosis. Seluruh kelinci percobaan diadaptasikan dengankondisi lingkungan penelitian selama 7 hari dengan pakan pellet dan air minum ad libitum. Kelincidipelihara dalam kandang individual. Enam puluh ekor kelinci tersebut dibagi secara acak menjadi 3kelompok masing-masing 20 ekor. Kelompok I sebagai kontrol (K-0) diberi 1,0 ml akuades/ekor per oral,kelompok II (K-10) diinfeksi 10x106 ookista E. magna/ekor per oral dosis tunggal, dan kelompok III (K-20)diinfeksi 20x106 ookista E. magna /ekor per oral dosis tunggal. Pascainfeksi setiap hari 4 ekor per kelompokdianestesi dengan uretan (1,55 g/kg BB dalam larutan 25%, secara intraperitoneal), kemudian dibedah,diambil segmen usus halus (duodenum, jejunum, dan ileum). Segera setelah itu kelinci dibunuh dengancara dislokasi cervikal. Segmen usus halus diukur gerak peristalsis secara elektromiografik. Selanjutnyasampel tersebut dibuat preparat histokimia dengan teknik pewarnaan Nicotinamide Adenine DincleotidePhosphate-diaphorase (NADPH-d) untuk mengetahui jumlah neuron mienterik nitrergiknya. Data frekuensidan amplitudo kontraksi peristalsis, dan jumlah neuron mienterik nitrergik usus halus kelinci dianalisissecara statistika dengan sidik ragam dan uji-t (LSD). Hasil penelitian menunjukkan bahwa infeksi10x106 dan 20x106 ookista E. magna dapat menyebabkan timbulnya peningkatan frekuensi peristalsisusus halus (p<0,01), penurunan amplitudo kontraksi usus halus (P<0,01), dan penurunan jumlah neuronmienterik nitrergik usus halus (P<0,01) dibandingkan kelompok kontrol (K-0). Dari hasil disimpulkanbahwa infeksi ookista E. magna dapat menyebabkan peningkatan frekuensi peristalsis, penurunanamplitudo kontraksi, dan penurunan jumlah neuron mienterik nitrergik usus halus kelinci.
Karakteristik dan Patogenisitas Streptococcus Agalactiae Tipe ?-hemolitik dan Non-hemolitik pada Ikan Nila Esti Handayani Hardi; Sukenda -; Enang Harris; Angela Mariana Lusiastuti
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Streptococcus agalactiae was isolated from cultured Nile tilapia (Oreochromis niloticus) in Cirata gulfand Klaten. The isolates were Gram positive cocci, oxidative fermentative positive, motility, and catalasenegative, grown on media containing NaCl 6.5%, ?-haemolytic and non-haemolytic. Two types of S. agalactiae(?-haemolytic and non-haemolytic) are different from their variety of sugars fermentation. Strains ?-haemolytic can ferment more sugars, including arabinose, sorbitol, lactose, and trehalose. Experimentalinfectivity trials on Nile tilapia (size 15 g), non-haemolytic type showed more virulent. This type causedfaster mortality, more severe behavior changes, and pathology changes than â-haemolytic type. NonhemoliticS. agalactiae caused 48% mortality 6-24 hours after injection, whereas â-haemolitic type caused17% mortality which it occured in 48 hours after injection (mortality of fish control 2,22%). Behaviordisease signs caused by non-haemolitic S. agalactiae started to happen 6 hours after injection whereas 12hours in ?-haemolytic type infection. Histopatological changes were observed on fish eye, spleen, andbrain. Hyperaemia, hyperthrophi, degeneration, and necrosis were also found on infected fish. Thisresearch was concluded that non-haemolytic of S. agalactiae was more virulent than ?-haemolytic.
Ekstrak Air Umbi Ubijalar Ungu Menurunkan Total Kolesterol serta Meningkatkan Total Antioksidan Darah Kelinci I Made Jawi; Ketut Budiasa
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

High cholesterol diet can increase blood total cholesterol and the incidence of cardiovascular disease.Flavonoid from fruits and vegetables can prevent those conditions due to it’s antioxidant effect. The aimof this study was to examine hypolipidemic and antioxidant possible effects of water extract of purplesweet potato, on blood of local rabbit with high cholesterol diet. This studies used 20 female adult localrabbit which were divided into 2 groups by using randomized pre-test and post-test control group design.Before treatment blood samples were collected from auricula venous to examine the total cholesterol, LowDensity Lipoprotein(LDL), High Density Lipoprotein (HDL), triglyseride, malondialdehide (MDA), totalantioxidant, Serum Glutamic Oxaloacetic Transaminase (SGOT), Serum Glutamic Piruvic Transaminase(SGPT), and haemoglobin concentration, as pretest data. After pre-tests, group 1 was given high cholesteroldiet without water extract of purple sweet potato. In contrast group 2 was given high cholesterol diet withwater extract of purple sweet potato. After 3 months of the treatment the blood were collected for posttestexamination. The data was analysed by using t-test. The result showed a significant increase in totalcholesterol, LDL, SGOT,SGPT, and MDA in the blood of group 1( p<0,05), whereas there was a significantdecrease in HDL at the same group (p<0,05). However, after 3 months of the treatment, all of the parametersin group 2 (except the total cholesterol) were not significantly different with the pretest result (p>0.05),although there was a significant difference compared to the control group (p<0.05). Total cholesterol wassignificantly decreased after 3 months of treatment in group 2 (p<0,05). It can be concluded that waterextract of purple sweet potato can keep the lipid profiles in normal range values, prevent the increase ofMDA, increase blood total antioxidant, decrease blood total cholesterol, and safe for the rabbit liver thatgiven high cholesterol diet.
Efficacy of Piperazine Dihydrochlloride Against Toxocara Vitulorum in Buffalo Calves Fadjar Satrija; Yusuf Ridwan; Elok Budi Retnani
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was conducted to evaluate the efficacy of piperazine dihydrochloride against natural infectionof Toxocara vitulorum in buffalo calves. In the first trial 60 based on fecal ascarid egg count and bodyweight naturally infected calves were, allocated into four groups. Three groups (groups B, C and D) weregiven piperazine dihydrochloride per os at dose levels of 200, 300 and 500 mg per kg body weight,respectivelythe remain group is non-treated controls. Piperazine treatment reduced egg excretion levels at98 to 99 per cent within 7, 14 and 21 days after treatment. Depite no significant differences were foundamong the three doses, highest egg count reductions were observed in calves given the drug 300 mg per kgbodyweight. The second trial were conducted using 10 additional calves. Piperazine significantly reducedT vitulorum egg excretion by 93% within 7 days post the treatment.
Studi Epidemiologi Agen Zoonosis Escherichia coli O157:H7 melalui Analisis Random Amplification of Polymorphic DNA (RAPD) I Wayan Suardana; Wayan Tunas Artama; Widya Asmara; Budi Setiadi Daryono
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Epidemiological studies of zoonotic agent Escherichia coli O157:H7 have been analyzed pheneticallyand or phylogenetically. In a phenetic classification, micoorganisms are arranged into groups (phena) onthe basis of high overall similarity using both phenotypic and genotypic methods without judgementaspect of its ancestry or evolutionary. Due to its importance to epidemiological aspect, the study of geneticvariation of isolates origin from some sources need to be conducted in order to trace the routes of infection.A total of 20 samples obtained from some sources i.e clinically human feces, non-clinically human feces,cattle feces, chicken feces, and beef feces were used in this study. The study was started by confirming allof the isolates using O157 latex agglutination test and H7 antiserum test, followed by genomic DNAanalysis by random amplification of polymorphic DNA /RAPD methods. RAPD results were analyzed using a simple matching coeficient (Ssm) and alogorhythm unweighted pair group method using arithmeticaverages (UPGMA) programe. Results showed there were range of genetic DNA from local isolates (75.1–99,6%) which was almost similar to ATCC 43894 control isolate. The highest similarity (99.6%) to ATCC43894 control was showed by SM-7(1) isolate obtained from cattle fecal and KL-68(1), isolate obtainedfrom clinically human fecal. In addition, KL-52(7) obtained from clinically human fecal had high similarity(99.6%) to MK-35 isolate obtained from chicken fecal. On the other hand, DS-21(4) and DS-16(2) isolatesthat were obtained from beef had high similarity (84.9%) to other isolates including ATCC 43894 controlisolate. The highest similarity of E. coli O157:H7 isolates that were obtained from cattle feces, beef, andchicken feces to human feces isolate indicated that there were both cattle and chicken were potentialreservoirs of the zoonotic agen which can be transmitted to human.
Kualitas Tulang Tikus Betina Normal yang Diberi Ekstrak Sipatah-patah pada Masa Pertumbuhan Sabri Mustafa; Nurhidayat -; Koeswinarning Sigit; Bambang Pontjo Priosoeryanto; Wasmen Manalu
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The experiment was designed to study the effects of sipatah-patah extract (ESP) on bone growth ingrowing female rats in order to prevent osteoporosis during postmenopausal period. Twenty growing femaleSprague Dawley rats in similar body weight were used with the average of age was 20 days old. Theexperimental rats were randomly divided into 5 (five) groups: control group (NOV-0, administered carboxylmethyl cellulose 1%) and groups administered ESP at the age of 30 days (NOV-1), 60 days (NOV-2), 90days (NOV-3), and 120 days (NOV-4) with dosage of 750 mg/kg body weight daily. Blood samples werecollected every 30 days to analyze serum calcium and phosphate concentrations. At the end of the treatment(180 day old), all of the animals were sacrificed to measure and analyze bone growth histologically. Ostibia-fibula slice was stained by using Hematoxylin Eosin (HE) method to observe osteoblast and osteoclastdensities, while Masson trichrome was used to observe trabeculae structure. The results showedadministration of ESP in rat during growing period improved bone growth. NOV-1 with longer duration(150 days) showed better growth rate with longer femur, optimum serum calcium, and phosphateconcentrations, and higher number of osteoblast with lower osteoclast densities compared to control rats(NOV-0). It was concluded that giving ESP at earlier age with longer duration during growth period couldimprove bone growth. This condition was expected could also improve the bone conditions during growingperiod.
Urinalisis Menggunakan Dua Jenis Dipstick (Batang Celup) pada Sapi Bali Iwan Harjono Utama; Evi Marieti Hutagalung; I Wayan Puspa Ari Laxmi; I Gusti Made Krisna Erawan2,; Sri Kayati Widyastuti; Luh Eka Setiasih; Ketut Berata
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study aimed to compare two kind of urinalysis dipsticks normaly used for human, for used in balicattle. A total of 100 urine samples were collected from 78 bulls and 22 cows ages between 4-7 years old.Each sample was kept in a plastic bottle and labelled until urinalysis was performed. Both dipstickshowed different urin chemical analysis results, except for nitrite. However, in general both dipsticksshowed consistency urinalysis results. Therefore, the two dipsticks can be used for urinalysis in balicattle.
Preservasi Ovarium dan Pengaruhnya Terhadap Morfologi Folikel Domba Bayu Rosadi; Mohamad Agus Setiadi; Dondin Sajuthi; Arief Boediono
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The purpose of this study was to evaluate the effect of cooling and freezing of ewes ovarian tissue ontheir follicles morphology. The study was carried out in two experiments. Experiment I, ovaries weremaintained in Phosphate Buffered Saline (PBS) at -20oC and room temperature (RT) for 24 h, and at 5oCfor 24 h and 72 h, respectively. After storage, follicles were histologically evaluated. Experiment II, theovarian cortex was isolated and tissue slices (±1 mm3) were prepared. Following this tissues were loadedinto hemistraw then transferred to equilibration solution (PBS+20% FBS+7,5% EG+7,5 % DMSO) atroom temperature and held for 10, 20, 30 minutes, respectively. Afterward tissues were tranferred tovitrification solution (PBS+20%FBS +15%EG+15%DMSO ) for 3 minutes, then the hemistraw was placeddirectly into liquid nitrogen. After thawing, the tissues were prepared for histological examination. All ofthe follicles were deteriorated after 24 h storage at RT. The percentage of morphologically normal follicleswas significantly reduced when ovarian tissues were stored at -20oC for 24 h and at 5oC for 24 h and 72 h.However, it seemed to have a minor deterioration effect when the tissues were kept at 5oC for 24 h(P<0.05). Antral follicles were damaged in all of the treatments. Primordial follicles preserved theirmorphology intactness better than growing follicles. Exposing tissues to equilibration medium for 10minutes seemed to produce higher numbers of morphologically normal follicles (P<0.05), compared towhen tissues were exposed for 20 minutes and 30 minutes (P>0.05). It can be concluded that exposingtissues to equilibration solution for 10 minutes prior to freezing would kept the ovarian follicles morphologyin good condition.
Tepung Rumput Laut (Eucheuma Cottonii) Menaikkan Level Superoksida Dismutase (Sod) Ginjal Tikus Hiperkolesterolemia Tutik Wresdiyati; Ans Budi Hartanta; Made Astawan
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Hypercholesterolemia condition was reported can decrease activities of antioxidant superoxidedismutase, catalase, and glutathione peroxide. This study was conducted to observe the role of seaweed(Eucheuma cottonii) powder in increasing the level of antioxidant cooper, zinc-superoxide dismutase (Cu,Zn-SOD) in kidney tissues of hypercholesterolemic rats. Twenty male Wistar rats were used in this study.Those rats were divided into four groups ; (1) Negative control group (A), (2) and (3) werehypercholesterolemic groups that were treated with 5% (B), and 10% (C) of seaweed powder, and (4)Hypercholesterolemic group as positive control (D). The treatment was carried out for 35 days.Hypercholesterolemia condition (>130 mg/dl), except for group A, was achieved by feeding the rat withcommercial diet containing 1% cholesterol and drinking water ad libitum for 40 days. Serum total cholesterolwas analyzed both before and after treatment. Rat kidneys were taken at the end of treatment, andprocessed by using paraffin embedding standard method. The tissues were then stained usingimmunohistochemical technique to Cu,Zn-SOD. The results showed the seaweed powder decreased serumtotal cholesterol, increased caecum cholesterol, repaired alteration of kidney tissues, and increased thelevel of antioxidant Cu,Zn-SOD in the kidney of hypercholesterolemic rats. Dietary fibers and antioxidantactivity of 10% seaweed powder was better than the of 5%.
Penentuan Siklus Estrus Berdasarkan Gambaran Sitologi Vagina dan Profil Hormon pada Rusa Timor Wilmientje Marlene Mesang Nalley; Ristika Handarini; Muhammad Rizal; Raden Iis Arifiantini; Tuty Laswardi Yusuf; Bambang Purwantara
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Determination of estrous cycle especially the length of estrous is an important role in improvingthe fertility and reproductive performance of farm animals. This study was aimed to observe thelength of estrous cycle and estrous period based on cytologic and estrogen (E2) and progesterone (P4)hormone profiles. Samples of vaginal smears were collected daily and stained with Giemsa. Bloodsamples were collected every two days using jugular vein implant catheter. The hormone profileswere analized by radio immuno assay. All data were analyzed descriptively. Results of this studyshowed that there were four lowest points percentage of parabasal epithelial cells with the averageof 14.25%, while the superficial epithelial cells showed four highest points (85.75%). The intervalsbetween lowest point of parabasal epithelial cell and the highest point of superficial cells were at 20,18, and 16 with the average of 18 days. This phenomenon showed a cyclical processed with almostthe same length of time of the actual an estrous cycle pattern in timor deer. The range of E2 concentration was 7.06 to 18.14 pg/ml and P4 concentration was 2.58 to 7.48 ng/ml. The intervaltime between the peak of E2 was 17 days which represented the estrous cycle of timor deer. It isconcluded that analysis of estrous cycle in timor deer can be detected by vaginal cytological andhormone analysis profiles.

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