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Journal : Majalah Kedokteran Bandung

Pemberian Ekstrak Bayam Merah (Amarantus Tricolor linn) Memperbaiki Ekspresi BDNF dan GLUR1 pada Area Hipokampus Tikus Muda yang Terpapar Anestesi Inhalasi Halothane dan Sevoflurane Argarini, Raden; Wigati, Kristanti Wanito; Herawati, Lilik; Riawan, Wibi; Rehatta, Nancy M.
Majalah Kedokteran Bandung Vol 48, No 3 (2016)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1578.665 KB) | DOI: 10.15395/mkb.v48n3.844

Abstract

Otak dalam tahap perkembangan memiliki tingkat kerentanan lebih tinggi terhadap zat anestesi dibanding dengan otak orang dewasa. Penelitian ini bertujuan mengamati pengaruh pemberian ekstrak bayam merah terhadap penanda biologis fungsi kognitif dan neuroplastisitas, yaitu Brain Derived Neurotrophic Factor (BDNF) dan AMPA Glutamate Receptor subunit 1 (Glur1) pada area hipokampus tikus yang terpapar anestesi inhalasi halothane dan sevoflurane. Rancangan penelitian adalah randomized posttest only control group. Penelitian ini dilakukan di Departemen Biokimia Fakultas Kedokteran Universitas Airlangga dan prosedur anestesi dilakukan di Rumah Sakit Hewan Fakultas Kedokteran Hewan Universitas Airlangga pada bulan Mei 2014–Oktober 2014. Subjek penelitian adalah 23 ekor tikus putih jantan (Rattus norvegicus) galur Wistar, umur 1–1,5 bulan, bobot kurang lebih 60–100 gram dengan kondisi sehat fisik. Subjek dibagi menjadi 5 kelompok, yaitu K (kontrol); K1 (halothane 1 MAC); K2 (bayam merah 800 mg/kgBB+Halothane 1 MAC); K3 (sevoflurane 1MAC) dan K4 (bayam merah 800 mg/kgBB+ Sevoflurane 1 MAC). Hasil penelitian menunjukkan adanya penurunan ekspresi BDNF pada pemberian halothane dan sevoflurane serta peningkatan kembali ke normal pada kelompok yang sebelumnya telah diberikan bayam merah sebelum terpapar gas anastesi (p=0,000). Ekspresi GLUR1 meningkat pada pemberian halothane dan sevoflurane dan penurunan kembali ke normal pada kelompok yang sebelumnya telah diberikan bayam merah sebelum terpapar gas anestesi (p=0,000). Simpulan penelitian ini adalah pemberian ekstrak bayam merah memiliki potensi untuk mencegah neurotoksisitas pada fungsi kognitif tikus yang terpapar gas anastesi. [MKB. 2016;48(3):148–54]Kata kunci: AMPA Glutamate Receptor subunit 1 (GLUR1), Brain Derived Neurotrophic Factor (BDNF), ekstrak bayam merah, halothane, sevofluraneRed Spinach Extract Administration (Amarantus Tricolor linn) in Young Rats Exposed to Inhalation Anesthetic Halothane and Sevoflurane Improves BDNF and GLUR1 Expressions in Hippocampus AreaIn the development stage, brain has a higher level of vulnerability than the adult brain to anesthetic agents. The aim of this study was to investigate the effects of red amaranth extract to brain derived neurotrophic factor (BDNF) and AMPA glutamate receptor subunit 1 (Glur1) expressions as biomarkers of cognitive and neuroplasticity in rat hippocampus areas exposed to inhaled anesthetics halothane and sevoflurane. The design of this study was a randomized posttest only control group. This study was conducted at the Department of Biochemistry Faculty of Medicine, Universitas Airlangga, and the anesthetic procedures were performed at the Veterinary Hospital, Faculty of Veterinary Medicine Universitas Airlangga during the period of May–October 2014. The subjects of this study were 23 white male rats (Rattus norvegicus) wistar strain, aged 1–1.5 months, weighted approximately 60–100 grams in a healthy physical condition. The subjects were divided into 5 groups, K (control); K1 (1 MAC halothane); K2 (red amaranth 800 mg/kgBW + 1 MAC halothane); K3 (1 MAC sevoflurane); and K4 (red amaranth 800mg/kgBW+1 MAC sevoflurane). The results of this study showed a decrease in the expression of BDNF when halothane and sevoflurane were administered. Red amaranth treatment prior to anesthetic gases exposure preserved and increased BDNF expression (p=0.000) while GLUR1 expression increased in the group that received halothane and sevoflurane administration and decreased back to normal in the group that received red amaranth treatment prior to exposure to anesthetic gases (p=0.000). In conclusion, red amaranth extract has the potential effect to prevent cognitive neurotoxicity on the cognitive function in rats exposed to anesthetic gases. [MKB. 2016;48(3):148–54]Key words: AMPA Glutamate Receptor subunit 1 (GLUR1), Brain Derived Neurotrophic Factor(BDNF), halothane, sevoflurane, red amaranth extract 
Co-Authors Adek Ardiansyah Aditiawarman - Ahmad Rukhani Lutfi Ahmad Rukhani Lutfi Angga Miftakhul Nizar Angga Miftakhul Nizar Anggis Putri Wijayanti Anggraeni Dyah Kumalasari Anissa Maulina Rinjani Annas, Jimmy Yanuar Anny Setijo Rahaju, Anny Setijo Ario Imandiri Ashon Sa’adi Ashon Sa’adi Bambang Harijono Bambang Purwanto Cantika Putri Melyana Chiquita Febby Pragitara Choesnan Effendi Citrawati Dyah Kencono Wungu Damayanti Tinduh David Wicaksono Dede Gantini Desi Rianti Rahmadhani Dicha Niswansyah Auliyah Dina Delvin Anggriani Dita Mega Utami Dwi Putri Rahayu Tampubolon Dwikora Novembri Utomo Eddy Rahardjo Eka Arum Cahyaning Putri Ema Qurnianingsih Endyka Erye Frety Ernawati Ernawati Ernawati Ernawati Faizah, Zakiyatul Fajar Shamsudin Fajar Syamsudin Farah Yusrania Gadis Meinar Sari Gantini, Dede Gebyar Catur Wahyuning Rohmawati Giwang Yayi Retnosari Gosy Endra Vigriawan Hanik Badriyah Hidayati, Hanik Badriyah HariSetijono Hayuris Kinandita Setiawan Herdy Sulistyono Hermina Novida Hidayat Arifin I Ketut Sudiana I'tishom, Reny Indira Syahraya Indriani, Aliza Umar Innani Wildania Husna Iqbal Laksana Irwanto, Irwanto Jimmy Yanuar Annas Junian Cahyanto Wibawa Krismaningrum, Veronika Intan Kusnanto Kusnanto Kusnanto Kusnanto Kusnanto Kusnanto Laylatul Fitriah Mukarromah Lila Tri Harjana Lina Lukitasari, M.Si M. Ali Machfud Maharani Ayuputeri Wijaya Mahmudin Aritanoga Maulana Arif Murtadho Maya Septriana Misbah Abdul Haris Nasution Alhanif Mohammad Anam Al-Ari Mohammad Anam Al-Arif Muchammad Rif’at Fawaid As’ad Muchammad Rif’at Fawaid As’ad Mufarrihul Ihsan Mufidah Sheena Andani Prastini Muhammad Miftahussurur Muhammad Zainul Arifin Nancy M. Rehatta, Nancy M. Ningrum, Astika Gita Niwanda Yogiswara Nugroho, Ardi Setyo Nuniek Nugraheni Sulistiawaty Nurcholis Majid Paulus Liben Paulus Liben Pradika Gita Baskara Prananda Surya Airlangga Purwo Sri Rejeki Purwo Sri Rejeki Purwo Sri Rejeki Purwo Sri Rejeki Purwo Sri Rezeki Putri Lisdiana Rahmawati Putri Rizkiya Raden Argarini Rehatta, Nancy Margarita Rezy Ramawan Melbiarta Rias Gesang Kinanti Rifka Kurniati Risa Etika, Risa Rivarti, Arina Windri Senja Himaya Senja Himaya Silvia Maya Ananta Siti Khaerunnisa Siti Khaerunnisa Soetojo Soetojo Sony Wibisono Sri Ratna Dwiningsih Suhartati Suhartati Sundari Indah Wiyasihati Susi Wahyuning Asih Tato Nuryanto Viskasari P. Kalanjati Wardhani, Indrayuni Lukitra Wibi Riawan Widati Fatmaningrum Widayana, Maeril Kirana Widjiati Wigati, Kristanti Wanito Windhu Purnomo Winna Adelia Amru Yudi Her Oktaviano Yusuf Salim Zulhabri Othman Zulhabri Othman