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All Journal Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Jurnal Medika Veterinaria Partner Jurnal Riset Akuakultur Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Jurnal Pengembangan Penyuluhan Pertanian Jurnal Ilmu Peternakan Terapan Jurnal Kedokteran Hewan

Joko Prastowo

Departemen Parasitologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta

Author-ID : 1470402

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary Other

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Molecular Detection of Toxoplasmosis Using Specific Primers P30, B1, and rDNA Wisnu Nurcahyo; Joko Prastowo; Ana Sahara
Jurnal Veteriner Vol 13 No 1 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Study in order to develop molecular techniques using specific primers for the early diagnosis oftoxoplasmosis have been conducted. Detection of Toxoplasma gondii genome was performed usingpolymerase chain reaction (PCR) technique. The primers used in this study were rDNA, P30, and B1. ThePCR products were further run using gel electrophoresis (gel 1.5% – 2.0%) and the band was documented.Toxoplasma was detected at 500 bp and 600 bp using primer P30 and B1, respectively. Whereas usingprimer rDNA no band was observed. It was assumed that primer rDNA was not sensitive since the targetamplification was 88 bp.

Strongyloides spp Distribution on Orangutans in Tanjung Putting National Park, Care Center in Pangkalanbun, and Sebangau National Park Wisnu Nurcahyo; Joko Prastowo
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Strongyloides spp is a parasitic nematode in livestock, primate and human which is considered asa danger zoonotic disease. Therefore, study about parasite distribution is very important in order to find outgenetic diversity among orangutan in quarantine, zoo and nature, as an effort to explore infection patternand life cycle of Strongyloides spp on orangutan. Amount of 326 orangutan feces were taken from threedifferent habitat of orangutan in Central Borneo, Tanjung Puting National Park, Orangutan Care Centerand Sebangau National Park. Samples which were collected from Tanjung Puting, Care Center and Sebangauwere 75, 80 and 171 respectively. Those samples were transported to the Parasitology laboratory in Facultyof Veterinary Medicine, Gadjah Mada University, Yogyakarta for examination and detection. Prevalence ofstrongyloides in Tanjung Putting, Sebangau and Orangutan Care Center were 24%, 14,6% and 13,3%respectively. Among positive samples of Strongyloides, 62,5% were from male orangutans, while 37,5% werefrom female orangutans. Strongyloides in pre adult and baby orangutan were 91,6% and 4,2% respectively.Meanwhile, Strongyloides in adult orangutan were very rare. Orangutan habitat in Sebangau National Parkis an ideal habitat for orangutan, supported by the watery condition of peat land, so that Strongyloides re-infection become difficult. Some factors may have important role in Strongyloidoses, such as behavior,physical condition, nutrition, age, body weight, sex, immunity and social status of orangutan.

Daya Larvasida Ekstrak Daun Muda Kedondong Hutan Terhadap Haemonchus contortus Secara In-vitro (LARVICIDAL EFFECT OF SPONDIAS PINNATA LEAF EXTRACT AGAINST HAEMONCHUS CONTORTUS IN VITRO) I Gusti Komang Oka Wirawan; Wisnu Nurcahyo; Joko Prastowo; Kurniasih Kurniasih
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study aimed to asses effective concentration of condensed tannins in the extract of Lannea grandis leaves (EDMKH) as larvacide anti-helminths on Haemonchus contortus. Experimental design used in this study was completely randomised design. The treatments group were administrated with 2.5%, 3.5% , and 4.5% EDMKH in the 0.2 g/mL solution, respectively. Moreover aqueduct was used for the negative control group, while 0.055% albendazole was applied to the positive control group. Variables measured were percentage of developed and undeveloped L1 in the incubation media. The data were the statistically analysed following General Linear Model format for completely randomised design. The software used in the analysis was SPSS v. 23.0 (IBM-SPSS 2015). Results showed that among the EDMKH treatments, the lowest larvacidal effect was recorded for 2.5% EDMKH treatment compared with 3.5% and 4.5% EDMKH. However, the value achieved by 2.5% EDMKH treatment was significantly higher (P<0.05) than that of negative control treatment. The highest larvacidal effect (100%) was shown by 4.5% EDMKH treatment which was not significantly different (P>0.05) from the positive control treatment. ABSTRAK Penelitian ini bertujuan untuk mengetahui konsentrasi efektif tanin kondensasi di dalam Ekstrak Daun Muda Kedondong Hutan (EDMKH) sebagai antelmintik yang bersifat larvasida terhadap Haemonchus contortus (H. contortus) secara in-vitro. Rancangan penelitian yang digunakan dalam penelitiam ini adalah Rancangan Acak Lengkap (RAL), dengan lima perlakuan yaitu tiga perlakuan EDMKH konsentrasi: 2,5%, 3,5%, 4,5% dari 0,2 g/mL larutan tersedia, satu kelompok kontrol (-) menggunakan air suling, satu kelompok kontrol (+) menggunakan albendazole konsentrasi 0,055%. Variabel yang diukur dan dianalisis: jumlah persentase L1 yang berkembang dan tidak berkembang dalam kelompok rendaman. Data persentase daya larvasida H. contortus dari konsentrasi tanin kondensasi EDMKH secara in-vitro dianalisis menggunakan analisis varian mengikuti prosedur Rancangan Acak Lengkap (RAL) menggunakan SPSS Versi 23(IBM-SPSS 2015). Hasil analisis statistika menunjukkan bahwa persentase larvasida EDMKH 2,5% nyata lebih rendah dibandingkan 3,5%, 4,5% dan albendazole 0,055% (P<0,05) tetapi lebih tinggi dari kontrol negatif. Konsentrasi efektif EDMKH terhadap larva H. contortus adalah konsentrasi 4,5%, memberikan efektivitas larvasida 100%, dan tidak berbeda nyata dengan kontrol positif (P>0,05).

Pengembangan Antibodi Poliklonal dari Stadium Oosista, Sporosista, dan Sporozoit Eimeria tenella (THE DEVELOPMENT OF POLYCLONAL ANTOBODY FROM EIMERIA TENELLA OOCYST, SPOROCYST, AND SPOROZOITE STADIUM) Galuh Tresnani; Joko Prastowo; Wisnu Nurcahyo; Budi Setiadi Daryono
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The research on developing diagnostic method, vaccine, and drugs for coccidiosis has been focused onthe finding of the immunogenic molecule in Eimeria. The identification of this agent will need the antibodywhich can recognize the biomolecule in the antigen. Antibody that has been developed for this purposeshould be analyzed first, and one of the simple methods for analyzing this antibody is through dot blotanalysis. The objective of this research was to analyze the polyclonal antibody which developed from theoocyst, sporocyst, and sporozoite of E. tenella using dot blot analysis. The antigen for this polyclonalantibody was made from each of the E. tenella stadium by sonication. Fifteen mice, divided into 3 groups,were then injected subcutaneously with each antigen. The sera from these mice were then collected, analyzedby using ELISA and then it will be used for the dot blot analysis. The research result showed that thepolyclonal antibody which has been developed in mice from each antigen can react with the antigen itself.From this result it can be concluded that the developing of this antibody is successful and it can be used forfurther research in immunoproteomic.

Diagnosis Toksoplasmosis Kongenital Berdasarkan Gen Surface Antigen-1 Toxoplama gondii Isolat Lokal Menggunakan Polymerase Chain Reaction (DIAGNOSIS OF CONGENITAL TOXOPLASMOSIS BASED ON SURFACE ANTIGEN -1 GENE OF LOCAL ISOLATE TOXOPLASMA GONDII USING POLY Dwi Priyowidodo; Sri Hartati; Asmarani Kusumawati; Joko Prastowo
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Congenital toxoplasmosis has an important role in the transmission of toxoplasmosis in animals andhumans. Thus, a rapid and an accurate diagnostic method is needed. The aim of this study was to conductthe diagnosis technique of congenital toxoplasmosis in mice based on surface antigen-1 (SAG-1) gene oflocal isolates (IS-1) T. gondii using Polymerase Chain Reaction (PCR). A total of 15 pregnant mice Balb/C strain with the aged of eight weeks were used as experimental animal. Mice were intraperitoneallyinfected with 103tachizoit of T. gondii RH strain at day 9th of gestation. Amniotic fluids, blood, fetus, andplacenta then were collected at day 1, 2 , 3, 4 and 5 post infection. DNA was extracted from the abovesamples using PureLinkTM Genomic DNA Kit (Invitrogen, Life Technologies, US), and then amplified byusing specific primer based on SAG-1 gene of the local isolate T. gondii. This study shows that positivePCR result were seen in all samples of amniotic fluids at day 2 up to day 5 post infection. Fetus andplacenta samples also show positive PCR result at 3 up to day 5 post infection. Negative PCR result showsin blood samples, however. To conclude, PCR technique using SAG-1 gene of local isolates T. gondii as atarget gene, could be used to detect congenital toxoplasmosis from infected mouse samples such as, amnionfluids, fetus, and placenta. Further research was needed to apply the PCR method with SAG-1 gene of localisolate T. gondiion the human samples of congenital toxoplasmosis.

Identifikasi Cacing Trematoda dan Gambaran Patologi Ginjal Burung Merpati yang Terinfeksi (IDENTIFICATION AND PATHOLOGICAL FEATURES OF TREMATODE IN PIGEON’S KIDNEY) Ana Sahara; Joko Prastowo; Dwi PriyoWidodo; Eryl Sri Rohayati; Sitarina Widyarini
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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This study is conducted to identify species of trematode and its pathological features in pigeons’kidney. Twenty five of Yogyakarta pigeons were examined for trematode infection in kidneys. One ofkidneys was mashed in mortar with a little water, the other was examined for histopathological featuresstained with hematoxyline-eosin.Trematodes found were stained with Schmison ´s Carmine. Seven pigeons(28%) were infected by trematode with non significant clinical features. Identification trematodescharacterized by oral sucker, pharynx, testes are slighty diagonal in position, irregular in shape and intracaecal. Ovary pretesticular and vitellaria widespread from anterior ovarium to the posterior body.Histopathological examination showed segment of trematode in the medullary collecting ducts lumen,dilatation, flattening and emptyness of ducts epithelial cells, emptyness, giant cells and dominationmononuclear cell in interstitial tissue, characterizing a granulomatous nephritis. Trematodes foundidentified as Paratanaisia bragai. The trematodes were found in very dilated medullary collecting ductsand caused inflammation in adjacent tissues. Further studies are needed to find out vector of trematodein pigeons .

Kekerabatan Genetik Caplak Rhiphicephalus (Boophilus) microplus Asal IndonesiaBerdasarkan Sekuen Internal Transcribed Spacer-2 (GENETIC RELATIONSHIP INDONESIAN RHIPHICEPHALUS (BOOPHILUS) MICROPLUS TICK BASED ON INTERNAL TRANSCRIBED SPACER-2 SEQUENSE ) Ana Sahara; Joko Prastowo; Rini Widayanti; Kurniasih .; Wisnu Nurcahyo
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Rhiphicephalus (Boophilus) microplus is important obligatory blood feeding ectoparasites transmittingmany different viral, bacterial and protozoan and plays a role as a vector of Babesia sp., The leria sp. andAnaplasma sp. in cattle. The accuracy in identifying and distinguishing interspecies and intraspeciesdiversity among parasites is needed to understand the epidemiology, biology and capacity as a vector.Variations in the DNA base sequence of the internal transcribed spacer region2 (ITS 2) has been used asa molecular marker for identification in an effort to determine phylogenetic relationships. The aim of thisstudy was to determine the ITS 2 gene nucleotide sequence of R. microplus, which was expected to beuseful for accurate identification the parasite diversity and phylogenetic relationship among many differentspecies. DNA amplification was conducted using BOO2 forward dan BOO2 reverse primers. The DNAsamples containing ITS2 region fragment of 1099 nt were derived from the nucleotide sequence multiplealignments of R.microplus and other ticks genes obtained from Gene bank using Clustal W software, andthen analyzed using the MEGA program version 6. Genetic distances based on nucleotide sequence weredetermined with Kimura 2-parameter method producing the smallest genetic distance of 0 % and 1.2 %.Construction of phylogenetic trees using the Neighbor joining method showed that ticks from variousregions in Indonesia was species complex which have a closer with R.microplus isolates from India, Laos,South Africa, China and Australia R.australis origin.

Profil Protein Stadium Sporozoit Eimeria tenella Isolat Yogyakarta Melalui Analisis Protein SDS-PAGE (PROTEIN PROFILE OF THE SPOROZOITE OF Eimeria tenella ISOLATES FROM YOGYAKARTA USING SDS-PAGE PROTEIN ANALYSIS) Galuh Tresnani; Joko Prastowo; Wisnu Nurcahyo; Budi Setiadi Daryono
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Coccidiosis is one of the important diseases in poultry industry. In Indonesia the morbidity of thedisease is between 80 to 90%. A rapid and prompt diagnosis would be one of the essential steps ineradication and control of the disease. The objective of this study is to determine the protein profile ofsporozoite of Eimeria tenella isolated in Yogyakarta using sodium dodecyl sulphate polyacrilamide gelelectrophoresis (SDS-PAGE) protein analysis. Protein analysis was performed in 12% polyacrilamide geland further electrophoresis at 100 volts and over-staining with Coomasie brilliant blue. The resultsshowed that the sporozoite of E. tenella isolated in Yogyakarta contained five proteins with molecularweights of 15, 26, 32, 80, and 91 kDa, respectively.

Identifikasi Clinostomum complanatum Secara Molekuler pada Ikan Air Tawar di Yogyakarta dan Riau (IDENTIFICATION OF Clinostomum Complanatum FROM FRESHWATER FISH IN YOGYAKARTA AND RIAU BASED ON MOLECULAR STUDY) Morina Riauwaty; Kurniasih .; Joko Prastowo; Windarti .
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The aim of study was, to identify Clinostomum complanatum (Digenea: Clinostomidae) infectingfreshwater fish in Yogyakarta and Riau on the bases of their molecular profiles in the internal transcribedspacer region (ITS1). Samples of climbing gouramy (Anabas testudineus) infected by Clinostomum sp. wereobtained from Kali Progo River, Yogyakarta. Whereas the climbing perch (Trichogaster trichopterus) wereobtained from the Sail River, Riau. Metacercariae of Clinostomum sp. found in the gills and visceralorgans were aseptically removed using needle, preserved in absolute ethanol. Molecular examination wasperformed by Polymerase Chain Reaction method consisted of extraction, amplification, electrophoresisand sequencing of DNA sample. The DNA sewuences of the samples were analysed by maximum parsimonyand neighbour-joining method. Phylogenetic analysis showed that Clinostomum sp. from Yogyakarta wasgenetically idential to Clinostomum complanatum, whereas Clinostomum sp. from Riau was geneticallysuspected as a new species (difference > 2%) which is included in one cluster to Clinostomum phalacrocorasis.

Gambaran Histopatologi Toksoplasmosis pada Kucing Peliharaan (HISTOPATHOLOGICAL FEATURES OF TOXOPLASMOSIS IN DOMESTIC CAT) Muhammad Hanafiah; Wisnu Nurcahyo; Joko Prastowo; Sri Hartati
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Study of histopathological changes of domestic cat organs which were serologically positive toxoplasmosis and laboratory infected which Toxoplasma have been undertaken. Histological section is prepared from organs including brain, liver, lung, kidney, duodenum, jejunum, ileum, and spleen then stained using hematoxylin and eosin (HE) and observed under microscope for histopathological changes. The results showed that in the serologically positive animals cell proliferation, infiltration of leucocyte and macrophage cells were observed in the ileum, whilst infiltration of eosinophil and leucocyte was seen in the kidney and liver. However, in other organ such as duodenum, jejunum, and spleen there were no changes observed. In cat experimentally infected with Toxoplasma, the infiltration of eosinophil cells were observed in the ileum and lung, while other organs such as kidney, liver, brain, jejunum, duodenum, and spleen showed no infiltration of inflammation cells. In conclusion, based on the results seropositive cat, showed proliferation of epithelial cells, leucocyte cells, and macrophage cells in the ileum, while in the lung, kidney, and liver showed infiltration of eosinophil and leucocyte. No infiltration of inflammation cells were observed in the brain, jejunum, duodenum, and spleen. ABSTRAK Penelitian mengenai histopatologi beberapa organ kucing peliharaan yang positif Toxoplasma baik secara serologi maupun yang diinfeksikan telah dilakukan. Tujuan penelitian adalah untuk mengetahui perubahan histopatologi pada organ kucing yang positif Toxoplasma. Data hasil pemeriksaan histopatologi yang terdapat pada preparat jaringan masing-masing organ dianalisis secara deskriptif dengan melihat gambaran perubahan histopatologi pada organ otak, hati, paru, ginjal, duodenum, jejenum, ileum, dan limpa. Hasil penelitian menunjukkan bahwa dengan menggunakan metode histopatologi organ kucing yang positif toksoplasmosis secara serologi teramati adanya proliferasi sel epitel, infiltrasi sel-sel leukosit dan makrofag pada ileum, ginjal, dan hati terlihat adanya infiltrasi eosinofil dan juga infiltrasi leukosit, sedangkan organ yang lain seperti jejenum, duodenum, dan limpa tidak teramati perubahan pada jaringan yang diperiksa. Sementara pada kucing yang dinfeksikan Toxoplasma, ileum dan paru teramati adanya infiltrasi sel-sel eosinofil, sedangkan organ lainnya seperti ginjal, hati, otak, jejenum, duodenum, dan limpa tidak teramati adanya infiltrasi sel-sel radang. Simpulan yang dapat ditarik adalah pada organ kucing yang positif toksoplasmosis teramati adanya proliferasi sel epitel, infiltrasi sel-sel leukosit, dan makrofag pada ileum, paru, ginjal dan hati teramati adanya infiltrasi eosinofil dan juga infiltrasi leukosit, sedangkan organ-organ lainnya seperti otak, jejenum, duodenum dan limpa tidak terlihat adanya infiltrasi sel-sel radang.

Co-Authors . Dwilantiani . Rianawati Alek Arisona Alfarisa Nururrozi Ambar Pertiwiningrum Ana Sahara Ana Sahara April Hari Wardhana Asmarani Kusumawati Awaludin, Aan Bambang Ariyadi Bambang Sutrisno Budi Purwo Widiarso BUDI SETIADI DARYONO Catur Sugiyanto Dwi Priyowidodo Dyah Nur Hidayati Eryl Sri Rohayati Galuh Tresnani I Gusti Komang Oka Wirawan I Gusti Komang Oka Wirawan, I Gusti Komang Kurniasih . Kurniasih . Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Margaretha Arnita Wuri Mufasirin Muhammad Hanafiah Nugraheni, Yudhi Ratna Okti Herawati Priyo Sambodo Priyowidodo P R. Wasito . Raden Wisnu Nurcahyo Rini Widayanti Sahara, Ana Sitarina Widyarini Soedarmanto Indarjulianto Soedarmanto Indarjulianto Sri Hartati Sudarmanto Indarjulianto Sumartono Sumartono Sumartono Sumartono Sunandjak . Surya Widyarsi Teguh Ari Prabowo Wida Wahidah Mubarokah Wida Wahidah Mubarokah Widiarso, Widiarso, B. P. Windarti . Windarti Windarti Wisnu Nurcahyo Wisnu Nurcahyo Wisnu Nurcahyo Wisnu Nurcahyo Yanuartono . Yosua Lede Pono

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