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Joko Prastowo
Departemen Parasitologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary Other

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Diagnosis Toksoplasmosis Kongenital Berdasarkan Gen Surface Antigen-1 Toxoplama gondii Isolat Lokal Menggunakan Polymerase Chain Reaction (DIAGNOSIS OF CONGENITAL TOXOPLASMOSIS BASED ON SURFACE ANTIGEN -1 GENE OF LOCAL ISOLATE TOXOPLASMA GONDII USING POLY Dwi Priyowidodo; Sri Hartati; Asmarani Kusumawati; Joko Prastowo
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Congenital toxoplasmosis has an important role in the transmission of toxoplasmosis in animals andhumans. Thus, a rapid and an accurate diagnostic method is needed. The aim of this study was to conductthe diagnosis technique of congenital toxoplasmosis in mice based on surface antigen-1 (SAG-1) gene oflocal isolates (IS-1) T. gondii using Polymerase Chain Reaction (PCR). A total of 15 pregnant mice Balb/C strain with the aged of eight weeks were used as experimental animal. Mice were intraperitoneallyinfected with 103tachizoit of T. gondii RH strain at day 9th of gestation. Amniotic fluids, blood, fetus, andplacenta then were collected at day 1, 2 , 3, 4 and 5 post infection. DNA was extracted from the abovesamples using PureLinkTM Genomic DNA Kit (Invitrogen, Life Technologies, US), and then amplified byusing specific primer based on SAG-1 gene of the local isolate T. gondii. This study shows that positivePCR result were seen in all samples of amniotic fluids at day 2 up to day 5 post infection. Fetus andplacenta samples also show positive PCR result at 3 up to day 5 post infection. Negative PCR result showsin blood samples, however. To conclude, PCR technique using SAG-1 gene of local isolates T. gondii as atarget gene, could be used to detect congenital toxoplasmosis from infected mouse samples such as, amnionfluids, fetus, and placenta. Further research was needed to apply the PCR method with SAG-1 gene of localisolate T. gondiion the human samples of congenital toxoplasmosis.
Identifikasi Cacing Trematoda dan Gambaran Patologi Ginjal Burung Merpati yang Terinfeksi (IDENTIFICATION AND PATHOLOGICAL FEATURES OF TREMATODE IN PIGEON’S KIDNEY) Ana Sahara; Joko Prastowo; Dwi PriyoWidodo; Eryl Sri Rohayati; Sitarina Widyarini
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study is conducted to identify species of trematode and its pathological features in pigeons’kidney. Twenty five of Yogyakarta pigeons were examined for trematode infection in kidneys. One ofkidneys was mashed in mortar with a little water, the other was examined for histopathological featuresstained with hematoxyline-eosin.Trematodes found were stained with Schmison ´s Carmine. Seven pigeons(28%) were infected by trematode with non significant clinical features. Identification trematodescharacterized by oral sucker, pharynx, testes are slighty diagonal in position, irregular in shape and intracaecal. Ovary pretesticular and vitellaria widespread from anterior ovarium to the posterior body.Histopathological examination showed segment of trematode in the medullary collecting ducts lumen,dilatation, flattening and emptyness of ducts epithelial cells, emptyness, giant cells and dominationmononuclear cell in interstitial tissue, characterizing a granulomatous nephritis. Trematodes foundidentified as Paratanaisia bragai. The trematodes were found in very dilated medullary collecting ductsand caused inflammation in adjacent tissues. Further studies are needed to find out vector of trematodein pigeons .
Kekerabatan Genetik Caplak Rhiphicephalus (Boophilus) microplus Asal IndonesiaBerdasarkan Sekuen Internal Transcribed Spacer-2 (GENETIC RELATIONSHIP INDONESIAN RHIPHICEPHALUS (BOOPHILUS) MICROPLUS TICK BASED ON INTERNAL TRANSCRIBED SPACER-2 SEQUENSE ) Ana Sahara; Joko Prastowo; Rini Widayanti; Kurniasih .; Wisnu Nurcahyo
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Rhiphicephalus (Boophilus) microplus is important obligatory blood feeding ectoparasites transmittingmany different viral, bacterial and protozoan and plays a role as a vector of Babesia sp., The leria sp. andAnaplasma sp. in cattle. The accuracy in identifying and distinguishing interspecies and intraspeciesdiversity among parasites is needed to understand the epidemiology, biology and capacity as a vector.Variations in the DNA base sequence of the internal transcribed spacer region2 (ITS 2) has been used asa molecular marker for identification in an effort to determine phylogenetic relationships. The aim of thisstudy was to determine the ITS 2 gene nucleotide sequence of R. microplus, which was expected to beuseful for accurate identification the parasite diversity and phylogenetic relationship among many differentspecies. DNA amplification was conducted using BOO2 forward dan BOO2 reverse primers. The DNAsamples containing ITS2 region fragment of 1099 nt were derived from the nucleotide sequence multiplealignments of R.microplus and other ticks genes obtained from Gene bank using Clustal W software, andthen analyzed using the MEGA program version 6. Genetic distances based on nucleotide sequence weredetermined with Kimura 2-parameter method producing the smallest genetic distance of 0 % and 1.2 %.Construction of phylogenetic trees using the Neighbor joining method showed that ticks from variousregions in Indonesia was species complex which have a closer with R.microplus isolates from India, Laos,South Africa, China and Australia R.australis origin.
Profil Protein Stadium Sporozoit Eimeria tenella Isolat Yogyakarta Melalui Analisis Protein SDS-PAGE (PROTEIN PROFILE OF THE SPOROZOITE OF Eimeria tenella ISOLATES FROM YOGYAKARTA USING SDS-PAGE PROTEIN ANALYSIS) Galuh Tresnani; Joko Prastowo; Wisnu Nurcahyo; Budi Setiadi Daryono
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Coccidiosis is one of the important diseases in poultry industry. In Indonesia the morbidity of thedisease is between 80 to 90%. A rapid and prompt diagnosis would be one of the essential steps ineradication and control of the disease. The objective of this study is to determine the protein profile ofsporozoite of Eimeria tenella isolated in Yogyakarta using sodium dodecyl sulphate polyacrilamide gelelectrophoresis (SDS-PAGE) protein analysis. Protein analysis was performed in 12% polyacrilamide geland further electrophoresis at 100 volts and over-staining with Coomasie brilliant blue. The resultsshowed that the sporozoite of E. tenella isolated in Yogyakarta contained five proteins with molecularweights of 15, 26, 32, 80, and 91 kDa, respectively.
Identifikasi Clinostomum complanatum Secara Molekuler pada Ikan Air Tawar di Yogyakarta dan Riau (IDENTIFICATION OF Clinostomum Complanatum FROM FRESHWATER FISH IN YOGYAKARTA AND RIAU BASED ON MOLECULAR STUDY) Morina Riauwaty; Kurniasih .; Joko Prastowo; Windarti .
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of study was, to identify Clinostomum complanatum (Digenea: Clinostomidae) infectingfreshwater fish in Yogyakarta and Riau on the bases of their molecular profiles in the internal transcribedspacer region (ITS1). Samples of climbing gouramy (Anabas testudineus) infected by Clinostomum sp. wereobtained from Kali Progo River, Yogyakarta. Whereas the climbing perch (Trichogaster trichopterus) wereobtained from the Sail River, Riau. Metacercariae of Clinostomum sp. found in the gills and visceralorgans were aseptically removed using needle, preserved in absolute ethanol. Molecular examination wasperformed by Polymerase Chain Reaction method consisted of extraction, amplification, electrophoresisand sequencing of DNA sample. The DNA sewuences of the samples were analysed by maximum parsimonyand neighbour-joining method. Phylogenetic analysis showed that Clinostomum sp. from Yogyakarta wasgenetically idential to Clinostomum complanatum, whereas Clinostomum sp. from Riau was geneticallysuspected as a new species (difference > 2%) which is included in one cluster to Clinostomum phalacrocorasis.
PREVALENSI DAN INSIDENSI PARASIT PADA IKAN MAS (CYPRINUS CARPIO) . Dwilantiani; Yudhi Ratna Nugraheni; Joko Prastowo; Dwi Priyowidodo; Ana Sahara; Wisnu Nurcahyo
Partner Vol 24, No 2 (2019): Edisi November 2019
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v24i2.285

Abstract

Infestasi parasit pada ikan dalam jumlah besar dapat menyebabkan kerugian ekonomi yang cukup signifikan. Parasit dari jenis Trematoda monogenean dan protozoa mendominasi infestasi tersebut.  Penelitian ini dilakukan untuk mendapatkan status kejadian infestasi parasit pada ikan mas biasa (Cyprinus carpio). Sebanyak 200 ikan mas (Cyprinus carpio) dikumpulkan dari kolam-kolam di Sleman dan Magelang, Jawa Tengah Indonesia. Metode pemeriksaan menggunakan metode wet mount. Parasit yang ditemukan sebagai berikut: Ichthyopthirius multifilis 41% Dactylogyrus  spp. 44,5% dan Gyrodactylus spp. 25%. Kejadian insidensi parasit sebagai berikut: Ichthyopthirius multifilis 1496/82, Dactylogyrus  spp. 119/37 dan Gyrodactylus spp. 2/2. Kondisi kolam yang buruk dan pergantian musim di Indonesia merupakan faktor yang sangat berpengaruh terhadap kejadian infestasi parasit pada ikan mas (Cyprinus carpio).
Gambaran Histopatologi Toksoplasmosis pada Kucing Peliharaan (HISTOPATHOLOGICAL FEATURES OF TOXOPLASMOSIS IN DOMESTIC CAT) Muhammad Hanafiah; Wisnu Nurcahyo; Joko Prastowo; Sri Hartati
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.81 KB) | DOI: 10.19087/jveteriner.2017.18.1.11

Abstract

Study of histopathological changes of domestic cat organs which were serologically positive toxoplasmosis and laboratory infected which Toxoplasma have been undertaken. Histological section is prepared from organs including brain, liver, lung, kidney, duodenum, jejunum, ileum, and spleen then stained using hematoxylin and eosin (HE) and observed under microscope for histopathological changes. The results showed that in the serologically positive animals cell proliferation, infiltration of leucocyte and macrophage cells were observed in the ileum, whilst infiltration of eosinophil and leucocyte was seen in the kidney and liver. However, in other organ such as duodenum, jejunum, and spleen there were no changes observed. In cat experimentally infected with Toxoplasma, the infiltration of eosinophil cells were observed in the ileum and lung, while other organs such as kidney, liver, brain, jejunum, duodenum, and spleen showed no infiltration of inflammation cells. In conclusion, based on the results seropositive cat, showed proliferation of epithelial cells, leucocyte cells, and macrophage cells in the ileum, while in the lung, kidney, and liver showed infiltration of eosinophil and leucocyte. No infiltration of inflammation cells were observed in the brain, jejunum, duodenum, and spleen. ABSTRAK Penelitian mengenai histopatologi beberapa organ kucing peliharaan yang positif Toxoplasma baik secara serologi maupun yang diinfeksikan telah dilakukan. Tujuan penelitian adalah untuk mengetahui perubahan histopatologi pada organ kucing yang positif Toxoplasma. Data hasil pemeriksaan histopatologi yang terdapat pada preparat jaringan masing-masing organ dianalisis secara deskriptif dengan melihat gambaran perubahan histopatologi pada organ otak, hati, paru, ginjal, duodenum, jejenum, ileum, dan limpa. Hasil penelitian menunjukkan bahwa dengan menggunakan metode histopatologi organ kucing yang positif toksoplasmosis secara serologi teramati adanya proliferasi sel epitel, infiltrasi sel-sel leukosit dan makrofag pada ileum, ginjal, dan hati terlihat adanya infiltrasi eosinofil dan juga infiltrasi leukosit, sedangkan organ yang lain seperti jejenum, duodenum, dan limpa tidak teramati perubahan pada jaringan yang diperiksa. Sementara pada kucing yang dinfeksikan Toxoplasma, ileum dan paru teramati adanya infiltrasi sel-sel eosinofil, sedangkan organ lainnya seperti ginjal, hati, otak, jejenum, duodenum, dan limpa tidak teramati adanya infiltrasi sel-sel radang. Simpulan yang dapat ditarik adalah pada organ kucing yang positif toksoplasmosis teramati adanya proliferasi sel epitel, infiltrasi sel-sel leukosit, dan makrofag pada ileum, paru, ginjal dan hati teramati adanya infiltrasi eosinofil dan juga infiltrasi leukosit, sedangkan organ-organ lainnya seperti otak, jejenum, duodenum dan limpa tidak terlihat adanya infiltrasi sel-sel radang.
SCANNING ELECTRON MICROSCOPY DARI Clinostomum complanatum (DIGENEA: CLINOSTOMIDAE) PADA IKAN BETOK ( Anabas testudineus ) DI YOGYAKARTA, INDONESIA Morina Riauwaty; Kurniasih Kurniasih; Joko Prastowo; Windarti Windarti
Jurnal Riset Akuakultur Vol 6, No 2 (2011): (Agustus 2011)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.476 KB) | DOI: 10.15578/jra.6.2.2011.303-309

Abstract

Penelitian ini bertujuan untuk mengetahui topografi permukaan Clinostomum complanatum (Digenea: Clinostomidae) yang menginfeksi ikan air tawar di Yogyakarta. Ikan betok (Anabas testudineus) diperoleh dari Kali Progo, Yogyakarta. Metaserkaria Clinostomum complanatum yang ditemukan di insang, dikeluarkan dengan menggunakan jarum dan diawetkan dalam etanol absolut. Pengamatan topografi permukaan tegument dengan Scanning Electron Microscopy (SEM). Data morfologi dianalisis secara deskriptif. Topografi tegument dari metaserkaria Clinostomum complanatum pada ikan betok menunjukkan adanya perbedaan struktur. Oral sucker terletak di ujung terminal, berbentuk elips dengan permukaan halus. Ventral sucker terletak dekat dengan oral sucker, berada di anterior dan memiliki papila sensoris, tetapi tidak memiliki spina. Permukaan tubuh cekung dan memiliki tonjolan yang tidak beraturan. Lubang ekskretori terletak di ujung posterior tubuh cacing.
AKTIVITAS LARVISIDAL EKSTRAK ETHANOL RUMPUT KEBAR (Biophytum petersianum Klotzsch) TERHADAP Aedes aegypti: LARVICIDAL ACTIVITY OF THE RUMPUT KEBAR (Biophytum petersianum Klotzsch) ETHANOL EXTRACT AGAINTS Aedes aegypti Priyo Sambodo; Joko Prastowo; Soedarmanto Indarjulianto
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 7 No. 1 (2012): JURNAL ILMU PETERNAKAN
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/jipvet.v7i1.37

Abstract

The aim of this research is to find out the larvicidal effect of rumput Kebar ethanol extract againts A. aegypti larvae. Seven hundred and twenty larvae of third instars were divided into 3 series and consist of 4 concentrations, (0 ppm, 2000 ppm, 2500 ppm and 3000 ppm) with 3 replications. Each group using 20 larvae of third instars were placed in 200 ml of treatment solutions. The effect of the treatments were monitored by counting the number of dead larvae after 24 h, 48 h and 72 h of exposure. Data was analyzed by ANOVA and Probit analysis was used to find out Lethal Concentration50 and LC90. The results showed that the average mortality is highest on the concentration of 3000 ppm after 72 h of exposure were 27 (36,15%). LC50 and LC90 values of 4770.626 ppm and 8264.651 ppm after 24 h of exposure.
Parasit Gastrointestinal pada Sapi di Daerah Aliran Sungai Progo Yogyakarta Yudhi Ratna Nugraheni; Dwi Priyowidodo; Joko Prastowo; Eryl Sri Rohayati; Ana Sahara; Aan Awaludin
Jurnal Ilmu Peternakan Terapan Vol 1 No 2 (2018): Jurnal Ilmu Peternakan Terapan
Publisher : Politeknik Negeri Jember

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Abstract

The aim of study was investigate gastrointestinal disease on cow fed forage from Progo watershed. Sixty three feses samples were taken from cows on Progo watershed. The method of feses analysis used was sentrifuge and Parfitt Bank. The results showed that 63 samples of cows feces were examined, among 25 were infected by Fasciola sp., 7 Paramphistomum sp., 14 Strongyle, and 9 Coccidiosis. The conclusion was the prevalence of gastrointestinal disease in cows was dominant incidence of Fasciolosis by 40%, Strongyle 22%, 14% Coccidiosis, and Paramphistomum sp.11%.
Co-Authors . Dwilantiani . Rianawati Alek Arisona Alfarisa Nururrozi Ambar Pertiwiningrum Ana Sahara Ana Sahara April Hari Wardhana Asmarani Kusumawati Awaludin, Aan Bambang Ariyadi Bambang Sutrisno Budi Purwo Widiarso BUDI SETIADI DARYONO Catur Sugiyanto Dwi Priyowidodo Dyah Nur Hidayati Eryl Sri Rohayati Galuh Tresnani I Gusti Komang Oka Wirawan I Gusti Komang Oka Wirawan, I Gusti Komang Kurniasih . Kurniasih . Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Kurniasih Margaretha Arnita Wuri Mufasirin Muhammad Hanafiah Nugraheni, Yudhi Ratna Okti Herawati Priyo Sambodo Priyowidodo P R. Wasito . Raden Wisnu Nurcahyo Rini Widayanti Sahara, Ana Sitarina Widyarini Soedarmanto Indarjulianto Soedarmanto Indarjulianto Sri Hartati Sudarmanto Indarjulianto Sumartono Sumartono Sumartono Sumartono Sunandjak . Surya Widyarsi Teguh Ari Prabowo Wida Wahidah Mubarokah Wida Wahidah Mubarokah Widiarso, Widiarso, B. P. Windarti . Windarti Windarti Wisnu Nurcahyo Wisnu Nurcahyo Wisnu Nurcahyo Wisnu Nurcahyo Yanuartono . Yosua Lede Pono