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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Teknologi Dan Industri Pangan Buletin Peternakan Jurnal Veteriner Jurnal Ilmu Ternak Jurnal Agripet Majalah Kedokteran Bandung Jurnal Ilmu dan Teknologi Hasil Ternak Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak dan Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Jurnal Ilmu dan Teknologi Peternakan Tropis Animal Production : Indonesian Journal of Animal Production Jurnal Aplikasi Teknologi Pangan Jurnal Aplikasi Teknologi Pangan Jurnal Ilmiah Ilmu-Ilmu Peternakan Jurnal Kedokteran Hewan
Irma Isnafia Arief
Departemen Ilmu Produksi Dan Teknologi Peternakan Fakultas Peternakan Institut Pertanian Bogor, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Veterinary Other

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Journal : Animal Production : Indonesian Journal of Animal Production

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The Ghrelin Receptor (Ghsr) Gene Polymorphism in Indonesian Local Chicken and Crossbreed Is Associated With Carcass Traits Khaerunnisa, Isyana; Jakaria, Jakaria; Arief, Irma Isnafia; Budiman, Cahyo; Sumantri, Cece
ANIMAL PRODUCTION Vol 19, No 2 (2017)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (943.521 KB) | DOI: 10.20884/1.jap.2017.19.2.596

Abstract

Ghrelin receptor (GHSR) gene is candidate gene for growth performance in chicken by modulating growth hormone release from the pituitary by binding to its ligand of ghrelin. Ghrelin gene, or growth hormone secretagogue (GHS) gene, is well known as feed intake and energy homeostasis regulator in mammals and birds. The objectives of this study were to identify the polymorphism of the T1857C GHSR locus in Indonesian local chicken and to evaluate its effects on carcass traits. The gene polymorphism was identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using Hin6I restriction enzyme. Effect of genotype on carcass composition was analyzed using SAS General Linear Model (GLM) procedure. The genotyping was performed on 343 individuals including Merawang, Pelung, Sentul, Kampung, broiler (strain Cobb, parent stock), F1 crossbred of Kampung x broiler (strain Cobb, parent stock), and F2 crossbred of Kampung x broiler (strain Cobb, parent stock). All individuals were successfully amplified and were resulted in a 470 bp PCR product. This locus was polymorphic with two alleles (T and C) and three genotypes (TT, CT, and CC). The T allele and TT genotype were predominant in all populations. Individuals with CT genotype were significantly had higher live weight at 26w, carcass weight, commercial cuts weights, and muscles weights than TT genotype in F2 crossbred of Kampung x broiler population. Association of the T1857C GHSR locus-polymorphism with chicken carcass composition has been described in Indonesian chicken, providing evidence that GHSR might be an important candidate gene for chicken carcass traits.
Co-Authors Amalina Nur Wahyuningtyas Anas Qurniawan Andi Febrisiantosa Andi Mutmainna Andi Nurul Mukhlisah Andi Tenri Bau Astuti Mahmud Apriadi Pasaribu Asti Yosela Oktaviana Ayu Lestari Bagus Priyo Purwanto Bahri Syamsuryadi Betty S. L. Jenie Bunga Putri Febrina Cahyo Budiman Cece Sumantri Damiana Rita Ekastuti Deni Fitra Deni Fitra Dondin Sajuthi Dwi Febiyanti - Edi Erwan El Latifa Sri Suharto Epi Taufik Faridah, Rajmi Fitri M Manihuruk Guslian Abdul Basir Hajrawati Hajrawati Hanifah N. Lioe Henny Nuraini Henny Nuraini Irmanida Batubara Isyana Khaerunnisa J. Hermanianto Jakaria Jakaria Jaya Putra Jahidin Lilis Nuraida Luci Cyrilla Luki Abdullah MADE ASTAWAN Mochammad Sriduresta Soenarno Muhamad Arifin Muhammad Fikri Al Habib Muhammad Nur Hidayat Muhammad Yusuf Murtini, Devi Ni Wayan Triwulandhari Niken Ulupi Noraimah Binti Sulaiman Noraimah Binti Sulaiman Noraimah binti Sulaiman Olfa Mega R A Maheswari R. R. A. Maheswari RIFKHAN RIFKHAN Rita Mutia Rudi Afnan Sadarman Sadarman Septi Dwi Utami Soenarno, M. Sriduresta sri murtini . Sri Rahmatul Laila Suharyanto Suharyanto Suharyanto Suharyanto Syah, Setiawan Putra Tuti Suryati Tuti Suryati Tuti Suryati Tuti Suryati Tutik Wresdiyati - Yantyati Widyastuti Yeni Setiorini Yeni Setiorini Zakiah Wulandari