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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences METAMORFOSA Journal of Biological Sciences Jurnal Ilmu Perairan (Aquatic Science) Jurnal Farmasi Andalas Jurnal Sains dan Teknologi Farmasi Jurnal Riset Kimia Indonesian Journal of Pharmaceutical Science and Technology INDONESIAN JOURNAL OF PHARMACY Jurnal Penelitian Pendidikan IPA (JPPIPA) JURNAL EDUCATION AND DEVELOPMENT JSFK (Jurnal Sains Farmasi & Klinis) JFIOnline Majalah Kedokteran Andalas Jurnal Farmasi Higea JURNAL PENELITIAN FARMASI & HERBAL Journal of Pharmaceutical and Health Research REACTOR : Journal of Research On Chemistry And Engineering
Akmal Djamaan
Universitas Andalas
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health

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Journal : Indonesian Journal of Pharmaceutical Science and Technology

 1 
Isolation of Secondary Metabolite A. niger “In-Habiting” Queen M. gilvus Hagen.’s Nest Alen, Yohannes; Melati, Atika; Sarina, Gemmy; Djamaan, Akmal
Indonesian Journal of Pharmaceutical Science and Technology Vol 5, No 2 (2018)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (524.816 KB) | DOI: 10.15416/ijpst.v5i2.15364

Abstract

Aspergillus niger is pathogen fungi that can live in various locations and can live contiguous with many hosts, one of them is queen termite’s nest. The aims of the study were to isolated the secondary metabilite of A.niger. Extraction proccess of secondary metabolite compounds was carried out by maceration method using methanol solvent. Based on that proccess, methanol extract was be yield 4,32% sample weight. Fractination proccess was carried out in the separating funnel using ethyl acetate solvent, which ethyl acetate fraction was be yield 14.39% methanol extract. Separation of the compounds was carried out by column chromatography method using n-hexane and ethyl asetate eluents. Purification of the compounds were done by recrystallization method using n-hexane and ethyl asetate. Two secondary metabolite compounds were successfully isolated from ethyl acetate fraction of the methanolic extract of fungus A. niger “In-Habiting” queen termite’s nest M. gilvus Hagen. Based on organoleptic examination, the compound signed AM-12-22-01 is 35 mg, white needle crystals, melting point 151-153 oC. While, the AM-12-60-01* is 15 mg, white needle crystals, melting point 91-93 oC. Based on the chemical analysis, thin layer chromatography, ultraviolet and infrared spectra data it was identified that AM-12-22-01 and AM-12-60-01 were a phenolic compounds.Key words: isolation, A. niger, In-Habiting, M. gilvus Hagen.
TLC Profile and Activity Test of Secondary Metabolites Aspergillus flavus “In-Habiting” Queen Termite’s Nest Macrotermes gilvus on Enriched Media Alen, Yohannes; Amelia, Rezki; Djamaan, Akmal
Indonesian Journal of Pharmaceutical Science and Technology Vol 5, No 1 (2018)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (589.946 KB) | DOI: 10.24198/ijpst.v5i1.14644

Abstract

Antibiotic are secondary metabolites yielded by microbe especially fungus. Previous research succesfull screened four kinds of fungus that live in termites queen’s nest, one of them was Aspergillus flavus. Furthermore, Alen et al (2016g) states that this fungus last to produce metabolite compounds on SDA media which only exist in first and second  subculture extract, omit gradually for the next culture. It was presumed happen caused a different habitat to grow. So it is necessary to enrich the media with queen termite nest to get back the initial metabolites. The enrichment was done using four different media concentrations (0.25; 0,50; 0.75 and 1 grams of nest/mL media). The results show that enrichment of 1 gram of nest/mL media provides the most optimum fungus growht. The third subculture of Aspergillus flavus is cultured on enriched media which will become the fourth subculture, this fungus cultured until tenth subculture, do extraction and   fracination to each culture. Based on TLC profile analysis, the initial metabolite not yet formed until tenth subculture, but forms six new stain terpenoid compounds.  The result of  columns chromatography obtained 10 sub-faction. Activity test was done by diffusion method to 12  test bacteria and 3 test fungus. Spot 2,5,6 (Rf 0.84; 0,36; 0,26) inhibit the growth of Pseudomonas aeruginosa ATCC 27853, spot 3,4 (Rf 0.74; 0,52) inhibit the growth of Micrococcus luteus ATCC 10240. The use of enriched media affect formation of  secondary metabolites Aspergillus flavus.  Keyword : Secondary metabolites, Aspergillus flavus, Macrotermes gilvus Hagen., Enriched media, TLC Profiles, Activity Assay
TLC Profile and Activity Test of Secondary Metabolites Aspergillus flavus “In-Habiting” Queen Termite’s Nest Macrotermes gilvus on Enriched Media Yohannes Alen; Rezki Amelia; Akmal Djamaan
Indonesian Journal of Pharmaceutical Science and Technology Vol 5, No 1 (2018)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (589.946 KB) | DOI: 10.24198/ijpst.v5i1.14644

Abstract

Antibiotic are secondary metabolites yielded by microbe especially fungus. Previous research succesfull screened four kinds of fungus that live in termites queen’s nest, one of them was Aspergillus flavus. Furthermore, Alen et al (2016g) states that this fungus last to produce metabolite compounds on SDA media which only exist in first and second  subculture extract, omit gradually for the next culture. It was presumed happen caused a different habitat to grow. So it is necessary to enrich the media with queen termite nest to get back the initial metabolites. The enrichment was done using four different media concentrations (0.25; 0,50; 0.75 and 1 grams of nest/mL media). The results show that enrichment of 1 gram of nest/mL media provides the most optimum fungus growht. The third subculture of Aspergillus flavus is cultured on enriched media which will become the fourth subculture, this fungus cultured until tenth subculture, do extraction and   fracination to each culture. Based on TLC profile analysis, the initial metabolite not yet formed until tenth subculture, but forms six new stain terpenoid compounds.  The result of  columns chromatography obtained 10 sub-faction. Activity test was done by diffusion method to 12  test bacteria and 3 test fungus. Spot 2,5,6 (Rf 0.84; 0,36; 0,26) inhibit the growth of Pseudomonas aeruginosa ATCC 27853, spot 3,4 (Rf 0.74; 0,52) inhibit the growth of Micrococcus luteus ATCC 10240. The use of enriched media affect formation of  secondary metabolites Aspergillus flavus.  Keyword : Secondary metabolites, Aspergillus flavus, Macrotermes gilvus Hagen., Enriched media, TLC Profiles, Activity Assay
Isolation of Secondary Metabolite A. niger “In-Habiting” Queen M. gilvus Hagen.’s Nest Yohannes Alen; Atika Melati; Gemmy Sarina; Akmal Djamaan
Indonesian Journal of Pharmaceutical Science and Technology Vol 5, No 2 (2018)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (524.816 KB) | DOI: 10.24198/ijpst.v5i2.15364

Abstract

Aspergillus niger is pathogen fungi that can live in various locations and can live contiguous with many hosts, one of them is queen termite’s nest. The aims of the study were to isolated the secondary metabilite of A.niger. Extraction proccess of secondary metabolite compounds was carried out by maceration method using methanol solvent. Based on that proccess, methanol extract was be yield 4,32% sample weight. Fractination proccess was carried out in the separating funnel using ethyl acetate solvent, which ethyl acetate fraction was be yield 14.39% methanol extract. Separation of the compounds was carried out by column chromatography method using n-hexane and ethyl asetate eluents. Purification of the compounds were done by recrystallization method using n-hexane and ethyl asetate. Two secondary metabolite compounds were successfully isolated from ethyl acetate fraction of the methanolic extract of fungus A. niger “In-Habiting” queen termite’s nest M. gilvus Hagen. Based on organoleptic examination, the compound signed AM-12-22-01 is 35 mg, white needle crystals, melting point 151-153 oC. While, the AM-12-60-01* is 15 mg, white needle crystals, melting point 91-93 oC. Based on the chemical analysis, thin layer chromatography, ultraviolet and infrared spectra data it was identified that AM-12-22-01 and AM-12-60-01 were a phenolic compounds.Key words: isolation, A. niger, In-Habiting, M. gilvus Hagen.
Co-Authors Agusri Boestari Andani Eka Putra Anthoni Agustien Arfiandi Arfiandi Asia Asia Asiska Permata Dewi Asiska Permata Dewi Asiska Permatadewi Atika Melati Bakhtra, Dwi Dinni Aulia Bilqis Nurganiyu Densi Selpia Sopianti Densi Selpia Sopianti Desma Yuni Dewi Gulyla Hari Dian Handayani Dini Hanifa Edtyva Monicha Elmi Sariani Hasibuan Erizal Zaini Fatimah Saidah Gustina ' Inayati Rahmi irfan mustafa Irfan Mustafa Krisyanella Krisyanella Krisyanella Krisyanella Kustiasih Lestari Laura Syahrul Lola Alviche Mahrani Napitupulu Majid, Mohammed Isa Abdul Maria Ulfa Marlina . Melati, Atika Melzi Octaviani Mimi Susanti Mohamed Isa Abd. Majid Mohd. Azizan Mohd. Noor Muslim Suardi Musyirna Rahmah Nst Netty Suharti Netty Suharty Noor, Mohd. Azizan Mohd. Novesar Jamarun Refilda Refilda Rendowaty, Agnes Rezki Amelia Rezki Amelia Rina Wahyuni Rini Agustin Rusmana Wijaya Setia Ningrat Rustini Rustini Rustini Rustini Salman - Salman - Sarina, Gemmy Sri Elfina Syamsuardi Syamsuardi Syukri Arief Syukria Ikhsan Zam Syukria Ikhsan Zam Witra Aulia Witra Aulia Yohannes Alen Yufri Aldi Yutra Noka