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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Penelitian Saintek ILMU KELAUTAN: Indonesian Journal of Marine Sciences Bioma : Berkala Ilmiah Biologi BERKALA FISIKA JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA Jurnal Natur Indonesia Jurnal Akademika Biologi Prosiding KPSDA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Indonesian Food and Nutrition Progress Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Media Farmasi Indonesia Jurnal Energi Baru dan Terbarukan AGRIC Open Access DRIVERset
Endang Kusdiyantini
Departemen Biologi Fakultas Sains Dan Matematika, Universitas Diponegoro, Semarang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences

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Journal : Biosaintifika: Journal of Biology

 1 
Screening Cellulolytic Bacteria from the Digestive Tract Snail (Achatina fulica) and Test the Ability of Cellulase Activity Wijanarka, Wijanarka; Kusdiyantini, Endang; Parman, Sarjana
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.7263

Abstract

On the research of enzyme production levels observed cellulase produced by bacteria in the digestive tract of the isolation of the Snail (Achatina fulica). Isolation of bacteria based on the ability of bacteria to grow on CMC media. The purpose of this study was to determine cellulase activity by cellulolytic bacteria. Some bacterial isolates were identified as cellulolytic bacteria, they were KE-B1, KE-B2, KE-B3, KE-B4, KE-B5, and KE-B6. Isolates KE-B6 was the best isolates. Furthermore KE-B6 isolates were grown on media production to determine the pattern of growth and enzyme activity. Measurement of cell growth was conducted by inoculating starter aged 22 hours at CMC production of liquid medium. Cellulase enzyme activity measurements was performed by the DNS method. The results showed that the highest activity by new isolate bacteria KE-B6 and its value of the activity of 0.4539 U/mL, growth rate () 0.377/hour and generation time (g) 1.84 hour. This research expected cellulase of producing bacteria were easy, inexpensive and efficient. This enzyme can be used as an enzyme biolytic once expected to replace expensive commercial enzyme. The biotylic enzyme can be applied to strains improvement (protoplast fusion).How to CiteWijanarka, W., Kusdiyantini, E. & Parman, S. (2016). Screening Cellulolytic Bacteria from the Digestive Tract Snail (Achatina fulica) and Test the Ability of Cellulase Activity. Biosaintifika: Journal of Biology & Biology Education, 8(3), 386-392.
Endophytic Bacteria from Faloak Plant Seed (Sterculia comosa) as Antibacterial Agent Moi, Maria Yasinta; Kusdiyantini, Endang; Pujiyanto, Sri
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.864 KB) | DOI: 10.15294/biosaintifika.v10i3.15361

Abstract

Endophytic bacteria isolated from some various kind of plants are able to yield some active compounds which have a role as an antibacterial compound. This work aimed to isolate and to screen the Endophytic bacteria from Faloak seed in its charge in inhibiting two kinds of pathogenic bacteria, Staphylococcus aureus and Escherichia coli. There were six isolates of Endophytic bacteria isolated in this work. According to the screening result, one isolate which had the most potential antibacterial activity (marked by the formation of inhibition zone) against S. aureus and E. coli. That most potential isolate was then tested and identified for both biochemical properties and molecular 16S rRNA gene. The result of this study showed that the endophytic bacteria isolate of Faloak seed with the code of S1 had the similarity with Enterobacter xiangfangensis strain 10-17 by 93 %. The research about endophytic bacteria of Faloak plants was never conducted before. Thus this research was expected to give information about the potential of antimicrobial ability Faloak plants which can be utilized in the discovery of new antibiotic compounds which in the future are expected to overcome the problem of microorganism resistance to antibiotics. The use of endophytic bacteria is expected to prevents the extinction of Faloak plants due to excessive use.
Identification of Gamma-Amino Butyric Acid Isolates Lactic Acid Bacteria Results from The Isolation of Rusip Prayitno, S Feta Avila; Suprihadi, Agung; Kusdiyantini, Endang
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.24995

Abstract

Gamma-aminobutyric acid (GABA) is a non-protein amino acid produced by glutamate decarboxylation by the enzyme glutamate decarboxylase and it is widely distributed in plants, animals and microorganisms. GABA-producing microorganisms including Lactic Acid Bacteria (LAB) which play a role in the fermentation process of food such as rusip. Rusip is a preserved food of marine fish made from raw anchovy of Bangka-Belitung. The purpose of this study was to obtain LAB isolates through the process of isolation from rusip and identify the presence of GABA. Isolation of LAB was carried out by the pour method on MRS agar and NA medium. The growing colonies were then characterized based on observations of colony morphology, Gram staining, catalase test, motility test, and fermentation type test. LAB isolates obtained were selected to obtain isolates which are capable to produce GABA using the Thin Layer Chromatography (TLC) method. The results of isolation from rusip and inoculation on MRS agar medium obtained, three LAB isolates namely RSP-A1, RSP-A2, and RSP-A3. Their characteristics are Gram positive bacteria, negative catalase test, negative motility test, and belong to homofermentative and heterofermentative bacteria groups. They were also able to produce GABA. Research on GABA from isolates isolated from rusip is a new thing in the world of GABA research. This research provides information that is beneficial to produce GABA easily, cheaply, and efficiently.
Co-Authors Adde Lolita Putri Adhitya Naufal Pribadhi Afrazak Johansyah Agni Rizqy Berliyanti Agung Suprihadi Ahmad Thontowi Anggraini, Ika Anggraini, Ika Anggrayeni, Yesti Tri Anto Budiharjo Arina Lunggani Arina Tri Lunggani Arina Tri Lunggani Arina Tri Lunggani Atit Kanti Basundari, Sinta Anas Bintoro Rudi Saputro, Bintoro Rudi Budi Raharjo Daniel Pasaribu Devia Kusmawati Arfina Dian Arif Rachman E Hugeng Wandono Elawati, Nunung Eni Elawati, Nunung Eni Enny Yusuf Wachidah Yuniwarti Erma Prihastanti Euis HERMIATI Evi F Simanjuntak Faradila Ayu, Near Putri Galih Pertiwi Akbar Ginting, R Cinta Badia H Hadiyanto Hadi Endrawati Hermin P Kusumaningrum Hermin P. Kusumaningrum Hermin Pancasakti Kusumaningrum Hilmi Fadhli Ihdina Isfara Suteja Ika Anggraini Ika Anggraini Anggraini Indah Sulistyarini Joedoro Sudarsono Khabib Khasan Alfaridhi, Khabib Khasan Kurniawati, Laily Laily Kurniawati Larasati, Ella Dewani Ledy Ginting Maria Sarah Fadillah MG Isworo Rukmi Moi, Maria Yasinta Muhammad Amal Nurhakim, Muhammad Amal Muhammad Nur Muhammad Nur Muhammad Z ainuri Muhammad Zainuri Munifatul Izzati N Nurhayati Naufal, Adhitya Nia Fadlilatul Laily Novi Alvita Pratama Nugrahaini, Dian Laila Nunung Eni Elawati Nurhayati Nurhayati Nurhayati Nurhayati Nurhayati Nurhayati Prayitno, S Feta Avila Prianto, A. Heru Puteri Aryani Rejeki Siti Ferniah Rida Yuliana, Rida Saniha Adini, Saniha Sarjana Parman Soni Nugraha Sri Pujianto, Sri Sri Pujiyanto Suprihadi Suprihadi TATI NURHAYATI Tri Winarni Agustini Triwibowo Yuwono Tyas Rini Saraswati Udi Tarwotjo, Udi Wijanarka Wijanarka Wijanarka, W