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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Penelitian Saintek ILMU KELAUTAN: Indonesian Journal of Marine Sciences Bioma : Berkala Ilmiah Biologi BERKALA FISIKA JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA Jurnal Natur Indonesia Jurnal Akademika Biologi Prosiding KPSDA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Indonesian Food and Nutrition Progress Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Media Farmasi Indonesia Jurnal Energi Baru dan Terbarukan AGRIC Open Access DRIVERset
Endang Kusdiyantini
Departemen Biologi Fakultas Sains Dan Matematika, Universitas Diponegoro, Semarang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences

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Journal : Jurnal Biologi Tropika

 1 
Isolasi dan identifikasi bakteri asam laktat dari pangan fermentasi berbasis ikan (Inasua) yang diperjualbelikan di Maluku-Indonesia Adde Lolita Putri; Endang Kusdiyantini
Jurnal Biologi Tropika Vol.1, No. 2, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (669.692 KB) | DOI: 10.14710/jbt.1.2.6-12

Abstract

Lactic acid bacteria are beneficial bacteria for the health of the body by improving the balance of the intestinal microflora. Lactic acid bacteria can generally be isolated from fermented foods, fruits, vegetables and meat. Inasua is a traditional fermented fish product originating from Central Maluku (Teon, Nila and Serua islands) wherein the fermentation process is carried out at room temperature for a certain time. The purpose of this study was isolation and identification based on the morphological characteristics of lactic acid bacteria in inasua. Isolation of Lactic Acid Bacteria was carried out using the pour plate method and the streak method on the media de Man, Rogosa and Sharpe Agar (MRSA) and Nutrient Agar media. The culture was incubated at 370C for 48 hours. The growing colonies were observed for morphological characteristics and GRAM staining of bacteria was carried out. The results of isolation of BAL from inasua in the media de Man, Ragosa and Sharpe Agar (MRSA) + CaCO3 obtained 4 bacterial isolates, namely INS-A1, INS-A2, INS-A3 and INS-A4 and only 2 isolates that have the characteristics of positive GRAM with Bacillus forms, namely INS-A2 and INS-A4. It is suspected that the 2 isolates were isolates of Lactic Acid Bacteria. The results of isolation on Nutrient Agar (NA) media contained 5 isolates namely INS-B1, INS-B2, INS B3, INS-B4, INS-B5 which have characteristics as negative GRAM shaped Bacillus
Karakterisasi morfologi, biokimia, dan uji enzimatis isolat khamir buah apel (Malus domestica Borkh.) yang berpotensi menghasilkan bioetanol Hilmi Fadhli; Endang Kusdiyantini; Nurhayati Nurhayati
Jurnal Biologi Tropika Vol. 2, No. 2, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1267.955 KB) | DOI: 10.14710/jbt.2.2.62-73

Abstract

Bioethanol can be produced by the fermentation using microorganisms such as yeast. The type of yeast that can produce bioethanol is still limited to certain yeast types such as Saccharomyces cerevisiae. Isolation and characterization of yeast need to be done to determine yeast isolates capable of producing bioethanol. Yeast can grow on a substrate that is rich in sugar as in the apple fruit. Enzymatic tests can support the ability to use on various substrates. This study aims to isolation, characterization, and enzymatic tests of yeast from apple that potential to produce bioethanol based on morphological and biochemical tests. The isolation method is done by using spread plates on PDA media. Morphological tests are carried out by making macroscopic (form, color, elevation, margin, the texture of colonies) and microscopic (form, budding, size of cells)observations of colonies and yeast cells. The growth was carried out on PDA at 6, 28, 37ᵒC, and also 50% glucose observed, Carbohydrate fermentation test (glucose, maltose, sucrose, lactose), and urease were used to the biochemicals characterization. Enzymatic tests were using amylase test, protease test, lipase test, and cellulase test. The bioethanol was measured bypycnometer method. The results of yeast isolation from apples were obtained 3 isolates, namely K1, K2, K3 with cell size 3,74 µm, 3,79 µm, and 3,41 µm respectively. Enzymatic test results showed that the presence of amylase enzyme activity in yeast. Based on morphological and biochemical characterization, K2 isolate was selected as genus Pichia sp. and able to producebioethanol by 5.6% (v / v).
Co-Authors Adde Lolita Putri Adhitya Naufal Pribadhi Afrazak Johansyah Agni Rizqy Berliyanti Agung Suprihadi Ahmad Thontowi Anggraini, Ika Anggraini, Ika Anggrayeni, Yesti Tri Anto Budiharjo Arina Lunggani Arina Tri Lunggani Arina Tri Lunggani Arina Tri Lunggani Atit Kanti Basundari, Sinta Anas Bintoro Rudi Saputro, Bintoro Rudi Budi Raharjo Daniel Pasaribu Devia Kusmawati Arfina Dian Arif Rachman E Hugeng Wandono Elawati, Nunung Eni Elawati, Nunung Eni Enny Yusuf Wachidah Yuniwarti Erma Prihastanti Euis HERMIATI Evi F Simanjuntak Faradila Ayu, Near Putri Galih Pertiwi Akbar Ginting, R Cinta Badia H Hadiyanto Hadi Endrawati Hermin P Kusumaningrum Hermin P. Kusumaningrum Hermin Pancasakti Kusumaningrum Hilmi Fadhli Ihdina Isfara Suteja Ika Anggraini Ika Anggraini Anggraini Indah Sulistyarini Joedoro Sudarsono Khabib Khasan Alfaridhi, Khabib Khasan Kurniawati, Laily Laily Kurniawati Larasati, Ella Dewani Ledy Ginting Maria Sarah Fadillah MG Isworo Rukmi Moi, Maria Yasinta Muhammad Amal Nurhakim, Muhammad Amal Muhammad Nur Muhammad Nur Muhammad Z ainuri Muhammad Zainuri Munifatul Izzati N Nurhayati Naufal, Adhitya Nia Fadlilatul Laily Novi Alvita Pratama Nugrahaini, Dian Laila Nunung Eni Elawati Nurhayati Nurhayati Nurhayati Nurhayati Nurhayati Nurhayati Prayitno, S Feta Avila Prianto, A. Heru Puteri Aryani Rejeki Siti Ferniah Rida Yuliana, Rida Saniha Adini, Saniha Sarjana Parman Soni Nugraha Sri Pujianto, Sri Sri Pujiyanto Suprihadi Suprihadi TATI NURHAYATI Tri Winarni Agustini Triwibowo Yuwono Tyas Rini Saraswati Udi Tarwotjo, Udi Wijanarka Wijanarka Wijanarka, W