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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vegetalika Jurnal Ilmu Pertanian Agroland : Jurnal Ilmu-Ilmu Pertanian Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology International Journal of Renewable Energy Development Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Journal of Tropical Soils Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Berkala Ilmiah Pertanian Jurnal Agro Teknologi Tropika Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Sains Tanah AGRIVITA, Journal of Agricultural Science agriTECH Indonesian Journal of Biotechnology Jurnal Tanah dan Air Jurnal Planta Tropika Pharmacon Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Jurnal Perlindungan Tanaman Indonesia INDONESIAN JOURNAL OF PHARMACY JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Caraka Tani: Journal of Sustainable Agriculture Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Agrin : Jurnal Penelitian Pertanian OLDI (Oseanologi dan Limnologi di Indonesia) jurnal Ilmu Pertanian (Agricultural Science) Jurnal Tanah dan Air (Soil and Water Journal) Kaunia: Integration and Interconnection of Islam and Science Journal Engagement: Jurnal Pengabdian Kepada Masyarakat
JAKA WIDADA
Departemen Mikrobiologi Pertanian, Fakultas Pertanian, Universitas Gadjah Mada
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Social Sciences Veterinary

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Journal : Agrin : Jurnal Penelitian Pertanian

 1 
SELEKSI MUTAN ANTIBIOSIS Bacillus subtilis B315 UNTUK PENGENDALIAN Ralstonia solanacearum Pr7 Prihatiningsih, Nur; Arwiyanto, Triwidodo; Hadisutrisno, Bambang; Widada, Jaka
Agrin : Jurnal Penelitian Pertanian Vol 18, No 1 (2014): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2014.18.1.213

Abstract

Bacillus subtilis B315 adalah bakteri antagonis terhadap patogen tanaman seperti Ralstonia solanacearum penyebab penyakit layu bakteri.  Salah satu mekanisme antagonisme adalah antibiosis.  Mutan antibiosis dibuat untuk membuktikan bahwa B. subtilis B315 mempunyai mekanisme antibiosis dalam mengendalikan R. solanacearum.  Tujuan penelitian adalah untuk menyeleksi mutan antibiosis B. subtilis B315 dibandingkan dengan B. subtilis B315 tipe alaminya, 2) mendeteksi sifat antibiosis dari  B. subtilis B315.  Metode yang digunakan adalah eksperimen melalui mutagenesis dengan EMS, seleksi mutan berdasarkan pengujian antibiosis, waktu generasi, pola nutrisi dan konsistensi koloni.  Sifat antibiosis dideteksi dengan ekstraksi metabolit sekunder.  Hasil penelitian menunjukkan bahwa mutagenesis dengan EMS yang optimum adalah pada waktu 274,7 menit dengan kematian maksimum 81,7%, Terdapat tiga kelompok mutan antibiosis yaitu yang tidak menghambat R. solanacearum, menghambat dengan zona hambatan 1-3 mm dan menghambat  dengan zona hambatan >3 mm. Mutan antibiosis terpilih yang kehilangan sifat  menghambat, namun waktu generasi dan pola nutrisi serta konsistensi koloni sama dengan B. subtilis B315 tipe alami adalah mutan M16.  Sifat antibiosis B. subtilis B315 ditunjukkan dengan metabolit sekunder yang diekstrak dengan metanol, menghasilkan puncak spot yang berbeda dengan mutan antibiosis M16. Kata kunci: Bacillus subtilis B315, mutan antibiosis, pengendalian, Ralstonia solanacearum  Pr7 ABSTRACT            Bacillus subtilis B315 is an antagonistic bacterium against plant pathogens such as Ralstonia solanacearum that causes bacterial wilt disease.  One of the antagonistic mechanisms is antibiosis. Antibiosis mutant is made to prove that B. subtilis B315 has an antibiosis mechanism in controlling R. solanacearum.  Aims of the research were 1) to select the B. subtilis B315 antibiosis mutant compared with B. subtilis B315 wild type, and  2) to detect antibiosis characters owned by B. subtilis B315.  The method used was an experiment through mutagenesis with EMS, mutant selection based on antibiosis test, generation time, nutrition pattern and colony consistency.  Antibiosis characters were detected by extraction of secondary metabolites.  Results of the research performed that optimal mutagenesis with EMS was at 274.7 minutes by maximum lethality of 81,7%.  There were 3 groups of antibiosis mutants i.e. not inhibiting R. solanacearum, inhibition with 1-3 mm of inhibiting zone, and inhibition with >3 mm of inhibiting zone.  The selected antibiosis mutant lost its inhibiting character, but the generation time and the nutrition pattern  and the colony consistency similar to B. subtilis B315 wild type was the M16 mutant.  Antibiosis characters of B. subtilis B315 were shown by secondary metabolites extracted with methanol to produce the peak spot that was different from the M16 antibiosis mutant. Key words: Bacillus subtilis B315, antibiosis mutant, control, Ralstonia solanacearum  Pr7 
Co-Authors , Tamad . Mustofa . Tohari A. Alimuddin Adi Laksono Ahmad Romdhon Akira Hosoyama Akira Hosoyama Ali Ikhwan Alim Isnansetyo Amekan, Yumechris Angga Prasetya Anna Safarrida Anna Safarrida, Anna Ariani Hatmanti Arif Muliawan Arifah Khusnuryani Arifah Khusnuryani Asrul Asrul Asrul Asrul Atsushi Yamazoe Atsushi Yamazoe Aziz Purwantoro Azwar Maas Azwar Ma’as Bambang Hadisutrisno Bambang Hadisutrisno Bambang Hadisutrisno Bambang HADISUTRISNO Bambang Hariwiyanto Bambang Hariwiyanto Bambang Hendro Sunarminto Bambang Hendro sunarminto Bostang Radjagukguk Camelia Herdini Christanti Sumardiyono Denny Irawati DEWI SESWITA ZILDA Dewi Seswita Zilda Dhani Suryawan Dhani Suryawan, Dhani Diani Mentari Diannastiti, Fani Aulia Didik Indradewa Didik Indradewa Didik Indradewa Dinar Mindrati Fardhani Dionysius Andang Arif Wibawa Dionysius Andang Arif Wibawa, Dionysius Andang Dody Kastono Dolly Iriani Damarjaya Dolly Iriani Damarjaya, Dolly Iriani Donny Widianto Donny Widianto Donny Widianto Donny Widianto Dyah Weny Respatie Edy Meiyanto Eka Tarwaca Susila Putra Eko Hanudin Eko Hanudin EKO IRIANTO Ema Damayanti Endang Semiarti Endang Sutriswati Rahayu Eni Harmayani ENI HARMAYANI Erni Martani Erni Martani Eti Nurwening Sholikhah Ganis Lukmandaru Gintung Patantis GINTUNG PATANTIS Gintung Patantis Hari Eko Irianto Hartono Hartono H, Hartono Hera Nirwati Hideaki Nojiri Hideaki Nojiri Indun Dewi Puspita IRFAN D. PRIJAMBADA Irfan Dwidya Prijambada Irwan Suluk Padang Jauhari Syamsiyah Joko Sulistyo Kana Ninomiya Keishi Senoo Keishi Senoo, Keishi Lucia Dhiantika Witasari Lucia Dhiantika Witasari, Lucia Dhiantika M. Mustofa Maria Gratias Masagus Muhammad Prima Putra Masaya Nishiyama Masaya Nishiyama, Masaya Melki Melki Mirtani Naima Mohamad Aji Ikhrami Muhammad Dylan Lawrie Muhammad Nur Cahyanto Muhammad Saifur Rohman Mujiyo Mujiyo Mukhlissul Faatih Mukhlissul Faatih1, Mukhlissul Mulyadi Mulyadi Mulyadi Mulyadi Mulyono Mulyono Murwantoko . Mustofa M, Mustofa Mustofa Mustofa N. Ngadiman Naima, Mirtani Nastiti Wijayanti Nastiti Wijayanti Nastiti Wijayanti Ngadiman . Ngadiman ., Ngadiman Ngadiman N, Ngadiman Nunuk Supriyatno Nur Edy Nur Prihatiningsih Ocky Karna Radjasa Oedjijono Oedjijono Pintaka Kusumaningtyas Prijambada, Irfan Dwidja PUSPITA LISDIYANTI Putra, Sukmana Siswandana Putu Sudira R. Riyanti Reni Nurjasmi Reni Nurjasmi, Reni Riska Wulansari Ristiarini, Susana Riyanti . Rusdi Evizal Sarto SATRIYAS ILYAS Shigeto Otsuka Shigeto Otsuka, Shigeto Shinta Hartanto Shogo Matsumoto Sigit Sunarta, Sigit SITI KABIRUN Siti Kabirun Siti Kabirun Siti Subandiyah Sofia Mubarika Sofia Mubarika H Sri Nopitasari Sri Nuryani Hidayah Utami Sri Nuryani Hidayah Utami Sri Nuryani Hidayah Utami, Sri Nuryani Hidayah Sri Suryanti Sri Suryanti Sri Wedhastri Stalis Norma Ethica Subagus Wahyuono Sudadi Sudadi Suhartiningsih Dwi Nurcahyanti Sumarno Sumarno Sumarno Sumarno Suryanti Suryanti Suryanti Suryanti Susila Herlambang TOHARI TOHARI Tohari Tohari Tomy Listyanto Tri Harjaka Tri Joko Raharjo Tri Rini Nuringtyas Tri Wibawa Tri Wibawa Triwibowo Yuwono Triwidodo Arwiyanto Wangi, Dyah Sekar A P WIDYA ASMARA Widya Asmara Wulansari, Riska Yani Lestari Nuraini Yani Lestari Nuraini, Yani Lestari Yasushi Yoshioka Yenny Sariasih Yose Rizal Yuli Setiawati Yuliana Prahastiwi Yuliana Yuliana Prahastiwi, Yuliana YUSRO NURI FAWZYA Yusro Nuri Fawzya Yusro Nuri Fawzya Yuuki Asano Yuyun Farida Yuyun Farida, Yuyun Zilda, Dewi Zeswita