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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vegetalika Jurnal Ilmu Pertanian Agroland : Jurnal Ilmu-Ilmu Pertanian Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology International Journal of Renewable Energy Development Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Journal of Tropical Soils Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Berkala Ilmiah Pertanian Jurnal Agro Teknologi Tropika Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Sains Tanah AGRIVITA, Journal of Agricultural Science agriTECH Indonesian Journal of Biotechnology Jurnal Tanah dan Air Jurnal Planta Tropika Pharmacon Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Jurnal Perlindungan Tanaman Indonesia INDONESIAN JOURNAL OF PHARMACY JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Caraka Tani: Journal of Sustainable Agriculture Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Agrin : Jurnal Penelitian Pertanian OLDI (Oseanologi dan Limnologi di Indonesia) jurnal Ilmu Pertanian (Agricultural Science) Jurnal Tanah dan Air (Soil and Water Journal) Kaunia: Integration and Interconnection of Islam and Science Journal Engagement: Jurnal Pengabdian Kepada Masyarakat
JAKA WIDADA
Departemen Mikrobiologi Pertanian, Fakultas Pertanian, Universitas Gadjah Mada
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Social Sciences Veterinary

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Journal : BIOTROPIA - The Southeast Asian Journal of Tropical Biology

 1 
GENOTYPIC AND PHENOTYPIC CHARACTERIZATION OF Alcaligenes javaensis JG3 POTENTIAL AS AN EFFECTIVE BIODEGRADER Stalis Norma Ethica; Oedjijono Oedjijono; Endang Semiarti; Jaka Widada; Tri Joko Raharjo
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 25 No. 1 (2018)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.448 KB) | DOI: 10.11598/btb.2018.25.1.583

Abstract

Utilization of glycerol by lipase producing bacteria offers great benefits for fat and oil waste degradation and waterwaste treatment. Nevertheless, there have been lack of reports about the availability of non-pathogenic, lipase producing bacteria, which could naturally degrade glycerol produced from the lipolysis process by lipase. This study reported a newly identified species of rhizobacteria, Alcaligenes javaensis JG3, which is not only able to produce high level of lipase, but also able to degrade glycerol molecules. Identification of strain JG3 was carried out using SEM (Scanning Electron Microscope), BD Phoenix 100 Automated Microbiology System and 16S rRNA gene analysis to determine its taxonomy status. The ability of the strain to metabolize glycerol was investigated both genotypically and phenotypically using degenerate PCR and a glycerol minimal medium. Identification test results showed that strain JG3 belongs to genus Alcaligenes, with the closest relationship with A. faecalis and A. aquatilis (96% nucleotide similarity maximum). Degenerate PCR resulted in a 248-bp sequence showing 93% similarity with glpK of Candidatus Sodalis pierantonius SOPE, a key gene involved in glycerol metabolism. In vitro glycerol utilization test result showed that Alcaligenes sp. JG3 was able to grow on glycerol aerobically, but not anaerobically. It is concluded that Alcaligenes sp. JG3 possesses genes coding for glycerol metabolism and this trait is phenotypically expressed, thus making the strain potential to be used as an effective fat and oil biodegrader.
Co-Authors , Tamad . Mustofa . Tohari A. Alimuddin Adi Laksono Ahmad Romdhon Akira Hosoyama Akira Hosoyama Ali Ikhwan Alim Isnansetyo Amekan, Yumechris Angga Prasetya Anna Safarrida Anna Safarrida, Anna Ariani Hatmanti Arif Muliawan Arifah Khusnuryani Arifah Khusnuryani Asrul Asrul Asrul Asrul Atsushi Yamazoe Atsushi Yamazoe Aziz Purwantoro Azwar Maas Azwar Ma’as Bambang Hadisutrisno Bambang Hadisutrisno Bambang Hadisutrisno Bambang HADISUTRISNO Bambang Hariwiyanto Bambang Hariwiyanto Bambang Hendro Sunarminto Bambang Hendro sunarminto Bostang Radjagukguk Camelia Herdini Christanti Sumardiyono Denny Irawati DEWI SESWITA ZILDA Dewi Seswita Zilda Dhani Suryawan Dhani Suryawan, Dhani Diani Mentari Diannastiti, Fani Aulia Didik Indradewa Didik Indradewa Didik Indradewa Dinar Mindrati Fardhani Dionysius Andang Arif Wibawa Dionysius Andang Arif Wibawa, Dionysius Andang Dody Kastono Dolly Iriani Damarjaya Dolly Iriani Damarjaya, Dolly Iriani Donny Widianto Donny Widianto Donny Widianto Donny Widianto Dyah Weny Respatie Edy Meiyanto Eka Tarwaca Susila Putra Eko Hanudin Eko Hanudin EKO IRIANTO Ema Damayanti Endang Semiarti Endang Sutriswati Rahayu Eni Harmayani ENI HARMAYANI Erni Martani Erni Martani Eti Nurwening Sholikhah Ganis Lukmandaru Gintung Patantis GINTUNG PATANTIS Gintung Patantis Hari Eko Irianto Hartono Hartono H, Hartono Hera Nirwati Hideaki Nojiri Hideaki Nojiri Indun Dewi Puspita IRFAN D. PRIJAMBADA Irfan Dwidya Prijambada Irwan Suluk Padang Jauhari Syamsiyah Joko Sulistyo Kana Ninomiya Keishi Senoo Keishi Senoo, Keishi Lucia Dhiantika Witasari Lucia Dhiantika Witasari, Lucia Dhiantika M. Mustofa Maria Gratias Masagus Muhammad Prima Putra Masaya Nishiyama Masaya Nishiyama, Masaya Melki Melki Mirtani Naima Mohamad Aji Ikhrami Muhammad Dylan Lawrie Muhammad Nur Cahyanto Muhammad Saifur Rohman Mujiyo Mujiyo Mukhlissul Faatih Mukhlissul Faatih1, Mukhlissul Mulyadi Mulyadi Mulyadi Mulyadi Mulyono Mulyono Murwantoko . Mustofa M, Mustofa Mustofa Mustofa N. Ngadiman Naima, Mirtani Nastiti Wijayanti Nastiti Wijayanti Nastiti Wijayanti Ngadiman . Ngadiman ., Ngadiman Ngadiman N, Ngadiman Nunuk Supriyatno Nur Edy Nur Prihatiningsih Ocky Karna Radjasa Oedjijono Oedjijono Pintaka Kusumaningtyas Prijambada, Irfan Dwidja PUSPITA LISDIYANTI Putra, Sukmana Siswandana Putu Sudira R. Riyanti Reni Nurjasmi Reni Nurjasmi, Reni Riska Wulansari Ristiarini, Susana Riyanti . Rusdi Evizal Sarto SATRIYAS ILYAS Shigeto Otsuka Shigeto Otsuka, Shigeto Shinta Hartanto Shogo Matsumoto Sigit Sunarta, Sigit SITI KABIRUN Siti Kabirun Siti Kabirun Siti Subandiyah Sofia Mubarika Sofia Mubarika H Sri Nopitasari Sri Nuryani Hidayah Utami Sri Nuryani Hidayah Utami Sri Nuryani Hidayah Utami, Sri Nuryani Hidayah Sri Suryanti Sri Suryanti Sri Wedhastri Stalis Norma Ethica Subagus Wahyuono Sudadi Sudadi Suhartiningsih Dwi Nurcahyanti Sumarno Sumarno Sumarno Sumarno Suryanti Suryanti Suryanti Suryanti Susila Herlambang TOHARI TOHARI Tohari Tohari Tomy Listyanto Tri Harjaka Tri Joko Raharjo Tri Rini Nuringtyas Tri Wibawa Tri Wibawa Triwibowo Yuwono Triwidodo Arwiyanto Wangi, Dyah Sekar A P WIDYA ASMARA Widya Asmara Wulansari, Riska Yani Lestari Nuraini Yani Lestari Nuraini, Yani Lestari Yasushi Yoshioka Yenny Sariasih Yose Rizal Yuli Setiawati Yuliana Prahastiwi Yuliana Yuliana Prahastiwi, Yuliana YUSRO NURI FAWZYA Yusro Nuri Fawzya Yusro Nuri Fawzya Yuuki Asano Yuyun Farida Yuyun Farida, Yuyun Zilda, Dewi Zeswita