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Journal : Jurnal Ilmu dan Teknologi Peternakan Tropis

Karakteristik Dendeng Lambok Khas Sumatera Barat dengan Metode Pengolahan dan Lama Penyimpanan yang Berbeda Bunga Putri Febrina; Tuti Suryati; Irma Isnafia Arief
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 6, No 1 (2019): JITRO, Januari
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (185.853 KB) | DOI: 10.33772/jitro.v6i1.5451

Abstract

ABSTRAKDendeng merupakan salah satu hasil pengolahan daging yang digemari masyarakat Indonesia. Salah satunya di Sumatera Barat terdapat dendeng yang dimodifikasi yakni dendeng lambok batokok. Tujuan penelitian ini untuk menganalis metode pengolahan yang tepat untuk menghambat proses oksidasi lemak dan kerusakan mikrobiologi pada suhu ruang, sehingga menghasilkan dendeng lambok yang berkualitas dan aman dikonsumsi. Variabel yang diamati meliputi sifat fisik (Kadar air, aktivitas air dan pH), sifat kimia (kandungan malonaldehida, antioksidan) dan analisis pertumbuhan mikroba (total plate count, S.aureus, E.coli, dan Salmonella). Rancangan penelitian yang digunakan adalah rancangan acak kelompok pola faktorial, dengan faktor pertama adalah metode pengolahan meliputi penggorengan dan pemanggangan, dan faktor kedua adalah waktu penyimpanan meliputi 0,2,4,dan 6 hari. Hasil penelitian menunjukkan bahwa metode pengolahan memberikan pengaruh yang nyata (P<0,05) kecuali pH. Lama penyimpanan berpengaruh nyata (P>0,05) kecuali pH dan kadar air. Hasil analisis mikrobiologi menunjukkan interaksi antara metode pengolahan dan lama penyimpanan. Berdasarkan penelitian ini, dendeng lambok batokok dengan metode pengolahan digoreng memiliki kadar air, aktivitas air dan pH yang lebih rendah, serta MDA dan aktioksidan yang lebih tinggi dibandingkan yang dipanggang. Dilihat dari segi mikrobiologi dendeng lambok masih aman dikonsumsi sampai hari kedua penyimpanan suhu ruang.Kata kunci: dendeng lambok, pengolahan, penyimpanan, fisikokimia, mikrobiologiABSTRACTDendeng is one of meat processing product preferred by Indonesian. One of them in West Sumatra, there is a modified dendeng namely dendeng lambok batokok. The purpose of this study was to analyze the appropriate processing methods to inhibit the process of fat oxidation and microbiological damage at room temperature, so as to produce high-quality dendeng lambok and safe for consumption. The variables observed included physical properties (moisture content, water activity, and pH), chemical properties (malonaldehyde content, antioxidants) and microbial growth analysis (total plate count, S. aureus, E. coli, and Salmonella). The study design used was a factorial randomized block design, with the first factor was the processing method including frying and roasting, and the second factor was the storage time including 0,2,4 and 6 days. The results showed that the processing method had a significant effect (P<0,05) except on pH. Storage duration had a significant effect (P>0,05) except on pH and water content. The results of the microbiological analysis showed that there was an interaction between processing methods and storage time. Based on this study, dendeng lambok batokok with frying process has lower water content, water activity and pH, and higher MDA and antioxidants than the roasting process. In terms of microbiology, dendeng lambok is still safe to consume until the second day of storage at room temperature.Keywords: dendeng lambok, processing, storage, physicochemical, microbiology
Antimicrobial activity of plantaricin IIA-1A5 on whey growth medium againts Pseudomonas aeroginosa: a scanning electron microscopy (SEM) study Andi Mutmainna; Irma Isnafia Arief; Ayu Lestari; Muhammad Nur Hidayat
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 2 (2022): JITRO, May 2022
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.24 KB) | DOI: 10.33772/jitro.v9i2.22444

Abstract

The study aimed to understand antimicrobial properties of plantaricin derived from L. plantarum IIA-1A5 and its inhibitory mechanism against Pseudomonas aeruginosa ATCC 27853 under SEM (scanning electron microscopy) observation. The inhibition of microbial growth was evaluated according to diameter of inhibitory zone. Scanning electron microscopy was then applied to observe the microstructure. The diameter of antimicrobial activity against Pseudomonas aeruginosa ATCC 27853 reached 6.52 mm in whey medium and 8.45 mm in whey+ medium (added with sucrose, tryptone and yeast extract). The experimental result clearly demonstrated the inhibition Pseudomonas aeruginosa ATCC 27853. Observation by SEM suggested that the antimicrobial activity of Pseudomonas aeruginosa ATCC 27853 related destruction of cellular morphology. The cell membrane was destroyed as indicated by the release of nucleic acid and reduction of membrane potential. In short, our experiment provided meaningful evidence on the future use of plantaricin IIA-1A5 as food preservative through disruption of cellular membrane causing leading to cell dying.Keywords:  Plantaricin, Lactobacillus plantarun, Pseudomonas aeruginosa, antimicrobial activity