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. Dachriyanus
Fakultas Farmasi, Universitas Andalas
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Journal : HAYATI Journal of Biosciences

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Anti-Inflammatory Effect of Isolate Compounds from Ethyl Acetate Fraction of Piper crocatum Ruiz and Pav Leaves on Lipopolysaccharide-induced RAW 264.7 Cells Nilda Lely; . Dachriyanus; Yufi Aldi; . Almahdy; Fatma Sri Wahyuni
HAYATI Journal of Biosciences Vol. 30 No. 1 (2023): January 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.1.28-34

Abstract

Red betel (Piper crocatum Ruiz and Pav) is a traditional Indonesian plant. The ethyl acetate fraction of red betel leaf has the potential as an anti-inflammatory. This research aimed to determine the anti-inflammatory effect of isolate compound from ethyl acetate fraction of Piper crocatum leaves on the production of cytokine pro-inflammatory TNF-α and ICAM-1 in lipopolysaccharide-induced RAW 264.7 cells. Cell viability was determined by the MTT method in the concentration range of 1.25; 2.5; 5; 10; 20, and 40 µg/ml. The TNF-α and ICAM-1 level was determined by the TNF-α and ICAM-1 assay kit as measured by an ELISA plate reader. Cell viability test of isolate compound from ethyl acetate fraction, the concentrations that gave viability percentages above 80% were 10; 5; 2.5 and 1.25 ug/ml. Isolate compound at concentrations of 10, 5, 2.5 and 1.25 ug/ml significantly inhibited TNF-α and ICAM-1 production with p<0.05 (p = 0.000).
Co-Authors ', Darwati ', Supriyatna . Almahdy . Ellyza . Yanwirasti A. Manaf Ali Abd. Rasyid Syamsuri Adriani Susanty Adriani susanty Adriani Susanty Agung Tri Sasongko Alimin, Nur Almahdy A Almahdy, . Amella Gusty Amri Bachtiar Darwati - Dewi, Citra Kartika Dian Dwiana Maydinar Dian Handayani Dian Sanita Putri Dira Hefni Ditya Kesumaningrum Dwisesaria Irma Lena Eka Roza Wijaya Ekaprasada, M. Taufik Elfita Elfita Elfita Elfita Elfita Elfita Elfita Elidahanum Husni Ellis Mawarni Elyunaida, Elyunaida Erizal Erizal Fatma Sri Wahyuni Fitra Yeni Gusty, Amella Hadi Ihsan Hadi Nasbey Haiyul Fadhli Harmawati Harmawati Hazli Nurdin Hazli Nurdin Hazli Nurdin Hazli Nurdin Henny Lucida Hj. Lajisc, Nordin Hj. Lajisc, Nordin Husein Hernadi Bahti Husen H Bahti Husen H. Bahti Husen H. Bahti - I Gusti Bagus Wiksuana Ihsan Ikhtiarudin Jufri Al Fajri Kamal Rullah Krisyanella Krisyanella Leni Merdawati Linda Eka Putri Lusianti, Mittha Lusianti, Mittha M. Taufik Ekaprasada M. Taufik Ekaprasada M. V. Sargent Madyawati Latief Magdazaleni, Magdazaleni Maisa, Esthika Ariany Masrul Md Nordin Hj Lajis Md Nordin Hj Lajis Merdawati, Leni Meri Ardianti Meri Susanti Meri Susanti Miftahul Husna Mira Febrina Muharni Muharni Muharni Muharni Muharni Muharni N. H. Ladjis Netty Suhatri Nilda Lely Nilda Tri Putri Nordin Lajis Novi Safitri Noviyandi Sayuti Nurdin, Hazli Nurdin, Hazli Pedro Anugrah Aswan Purnawan Pontana Putra Putra, Deddy Prima Putra, Deddy Prima Rahma Yulia Rahmat, Rosita Dewi Rahmat, Rosita Dewi Regina Handayani Handayani, Regina Handayani Rizal Fahmi Rob W.M van Soest Rosfita Rasyid Rustini Rustini Salman - Sanusi Ibrahim Sartika, Zikra Sartika, Zikra Saufitri, Dian Saufitri, Dian Sekar, Andisyah Putri Sofia, Sri Esky Suhatri Suhatri Supriyatna - Supriyatna Soetardjo Supriyatna Supriyatna Supriyatna Supriyatna Supriyatna Supriyatna Supriyatna Supriyatna Supriyatna Supriyatna Surya Dharma Suryati . Suryati Suryati Suryati Suryati Suryati Suryati Suryatir - Syofyan Syofyan Tadzkia Nisa Titen Yasril Veny Wahyu Saputri Vetty Priscilla Wijaya, Eka Roza Yanwirasti Yanwirasti Yanwirasti Yanwirasti Yasman, Yulia Yelly Oktavia Sari Yohannes Alen Yufi Aldi Yufri Aldi Yulia Yasman Yunazar Manjang Yunazar Manjang