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Journal : Jurnal Fitopatologi Indonesia

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Identifikasi Rizobakteria Asal Tanaman Cabai Berdasarkan Sekuen Gen 16S rRNA Jumsu Trisno; Yusniwati .
Jurnal Fitopatologi Indonesia Vol. 8 No. 3 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (381.476 KB) | DOI: 10.14692/jfi.8.3.79

Abstract

Rhizobacteria was reported to have a potential role as inducer of systemic resistance in several host plants. Five isolates of rhizobacteria (RbTD1-3, RbTD1-8, RbPdGN-3, RbAg1-5, RbLPK1-9) were isolated from rhizosphere of chillipepper plants in West Sumatera. Identification of rhizobacteria was conducted by polymerase chain reaction using primers 27F and 1525R which amplified 16S rRNA region. DNA fragment of 1.5 kb was succesfully amplified from 4 isolates (RbLPK1-9, RbPdGN-3, RbTD1-3 and RbTD1-8) and subjected to direct sequencing. Phylogenetic analysis based-on 16S rRNA sequences showed that isolate RbLPK1-9 has a close relationship with Pseudomonas fluorescen Biotipe G (92%), whereas isolates RbTD1-3, RbTD1-8 and RbPdGN-3 have a close relationship with Bacillus subtilis 10407 (57%).Key words: polymerase chain reaction, rhizobacteria, 16S rRNA
Co-Authors Afrizal Nofri Agus Sutanto Alfi Asben Anwar Kasim Anwar, Aswaldi Aprissilia Taifani Galuh Utomo Aprizal Zainal Auzar Syarif Ayu Kurnia Illahi Budi, Rahmad Setia Damayanti, Elok Deivy Andhika Permata Dendy Tri Dharma Putra Denny Yulfa Dewi Rezki Etti Swasti Fadilla Arishadea Febriani Febriani Gusti Marlina Gustian Gustian Gustian Haliatur Rahma, Haliatur Indri Yanil Vajri Irfan Suliansyah Jumsalia Jumsu Trisno KRISTINA, NILLA Lily Syukriani Magdalena Saragih Magdalena Saragih Saragih Mellyyana Handayani Mismawarni Srima Ningsih Nadia Khairunnisa Nalwida Rozen Neswati Neswati Netti Herawati Noflindawati Noflindawati Nova Reskhi Firdaus Novizar Nazir Nurbailis Obel, Obel P.K. Dewi Hayati Prana Dipa Tiarani Reni Mayerni Ryan Budi Setiawan Sahroni Ridoi Saragih Magdalena Siska Efendi Sobrizal Sobrizal Syahbanuari Sitompul Syukri Arif Trizelia . Yaherwandi