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., Yusniwati
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Journal : Journal of Applied Agricultural Science and Technology

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Effect of the Combination of the Growth Regulators and Putrescine on the Somatic Embryogenesis of Wheat (Triticum Aestivum L.) on Some Types of Explants Yusniwati Yusniwati; Ryan Budi Setiawan
Journal of Applied Agricultural Science and Technology Vol. 7 No. 4 (2023): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v7i4.158

Abstract

Plant breeding programs need to be carried out in order to improve the genetics of wheat that is able to adapt to tropical environments through hybridization, mutation induction, tissue culture, and genetic transformation.  In vitro culture through somatic embryogenesis pathways plays an important role in genetic improvement and its integration with other breeding programs can positively affect the improvement of wheat quality, quantity, and development in Indonesia. The purpose of this study was to obtain an embryogenic callus induction method from the Dewata variety using five different types of explants, namely mature Seeds, immature embryos, immature seeds, leaf,  stem, and to obtain combination of plant growth regulators and putrescine on somatic embryogenesis of wheat.   The experimental design was prepared based on a complete randomized design with a combination treatment of embryogenic callus induction media consisting of 9 levels, namely: 1 ppm 2.4- D, 1 ppm 2.4-D + 1 ppm Picloram, ppm 2.4-D + 1 mg / L Picloram, 1 ppm 2.4-D + 10-4  M Putrescine, 1 ppm 2.4-D + 1 ppm Picloram +10-4  M Putrescine, 2 ppm 2.4-D + 1 ppm Picloram + 10-4  M Putrescine, 1 ppm 2.4 D + 10-3 M Putrescine, 1 ppm 2.4 D + 1 ppm Picloram + 10-3 M Putrescine, 2 ppm 2.4 D + 1 ppm Picloram + 10-3 M Putrescine. The results showed that the media used was able to induce embryogenic callus using mature seed and immature embryo, but  immature seed and leaf were not able to produce embryogenic callus. The best media  that produced the highest percentage of embryogenic callus was 2 ppm 2.4-D + 1 ppm Picloram + 10-4  M Putrescine with as much as 85.9% in young embryo explants.
Co-Authors Afrizal Nofri Agus Sutanto Alfi Asben Anwar Kasim Anwar, Aswaldi Aprissilia Taifani Galuh Utomo Aprizal Zainal Auzar Syarif Ayu Kurnia Illahi Budi, Rahmad Setia Damayanti, Elok Deivy Andhika Permata Dendy Tri Dharma Putra Denny Yulfa Dewi Rezki Etti Swasti Fadilla Arishadea Febriani Febriani Gusti Marlina Gustian Gustian Gustian Haliatur Rahma, Haliatur Indri Yanil Vajri Irfan Suliansyah Jumsalia Jumsu Trisno KRISTINA, NILLA Lily Syukriani Magdalena Saragih Magdalena Saragih Saragih Mellyyana Handayani Mismawarni Srima Ningsih Nadia Khairunnisa Nalwida Rozen Neswati Neswati Netti Herawati Noflindawati Noflindawati Nova Reskhi Firdaus Novizar Nazir Nurbailis Obel, Obel P.K. Dewi Hayati Prana Dipa Tiarani Reni Mayerni Ryan Budi Setiawan Sahroni Ridoi Saragih Magdalena Siska Efendi Sobrizal Sobrizal Syahbanuari Sitompul Syukri Arif Trizelia . Yaherwandi