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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Online Mahasiswa (JOM) Bidang Ilmu Sosial dan Ilmu Politik JURNAL ILMU BUDAYA Jurnal Kimia Terapan Indonesia Edumaspul: Jurnal Pendidikan Jurnal Ilmu dan Teknologi Kayu Tropis JURNAL ILMU KEFARMASIAN INDONESIA Pharma Xplore : Jurnal Sains dan Ilmu Farmasi EduPsyCouns: Journal of Education, Psychology and Counseling Jurnal Media Akuntansi (MEDIASI) Makara Journal of Science

Muhammad Hanafi

Unknown Affiliation

Author-ID : 273841

Agriculture, Biological Sciences & Forestry Arts Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Environmental Science Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

Published : 28 Documents Claim Missing Document

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Antifungal Properties of Palepek Baringin Wood (Shorea leavis Ridl) Renhart Jemi; Wasrin Syafii; Fauzi Febrianto; Muhammad Hanafi
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 10, No 1 (2012): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

The objectives of this research were to evaluate the extractive substances of heartwood of palepek baringin wood (Shorea leavis Ridl) as a bio-active substance to wood destroying fungi i.e. Schizophyllum commune Fr and Pleurotus ostreatus. The 40 mesh of heartwood meals was extracted by methanol, chloroform, ethyl acetate, n-hexane and buthanol. The extractives obtained were subjected to wood destroying fungi S. commune and P. ostreatus, and preservative containing copper, chrome and boron (CCB) was used for comparison. The extractive of palepek baringin wood was dominated by ethylacetate fraction (3.54%), followed by chloroform and butanol fraction 1.73% and n-hexane (0.09%). All fractions have an antifungal activity to inhibit the growth of S. commune and P. ostreatus. Concentration of 50 ppm butanol fraction inhibited the growth of S. commune, and concentration of 250 ppm chloroform fraction inhited the growth of Pleurotus sp. Preservative of CCB 100 ppm inhibited the growth of S. commune and P. ostreatus by 43%. Butanol fraction contained 16 active compounds (PB.1-PB.16) that could inhibit the growth both of S. commune and P. ostreatus with IC50 54.29-64.32 and 48.40-65.86 ppm, respectively. Based on mass spectrometry (LC-MS) and 1HNMR analysis, the compound of PB.1.1 which has anti-fungal activity was 2,3-dihydroxyoctadecanoic acid.Key words: 2,3-dihydroxyoctadecanoic acid, antifungal, Pleurotus ostreatus, Schyzophlum commune, Shorea leavis

Anticancer Activity and Chemical Compounds of Suren Heartwood Extract (Toona sureni) Rita K Sari; Wasrin Syafii; Suminar A Achmadi; Muhammad Hanafi; Yanotama T Laksana
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 10, No 1 (2012): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

The aims of this research were to determine the yield extracts from continuous extraction of suren heatwood (Toona sureni) in n-hexane, ethyl acetate and methanol solvents, to evaluate this extracts using in vitro anticancer tests (antioxidant, brine shrimp lethality test /BSLT, antiproliferative effects to HeLa servical cancer cell lines, Raji limphoma cancer cell lines, and Vero normal cell lines), and to analyze the best extract based on the anticancer activities. This experiment showed that the yield of methanolic, ethyl acetate, and n-hexane extracts were 0.43%, 0.25%, and 0.18% respectively. The methanolic and ethyl acetate extracts had the high antioxidant activities with EC50 51 and 68 μg ml-1 respectively. Based on BSLT, the ethyl acetate extract was the most active extract (LC50 40 μg ml-1), it followed by the methanolic extract (LC50 116 68 μg ml-1 ), and the n-hexane extract (LC50 161 68 μg ml-1). Further testing showed that the ethyl acetate extract had high antiproliferative effects to Raji (IC50 31 μg ml-1) and HeLa (IC50 65 μg ml-1), but it was more secure against Vero cell lines (IC50 105 μg ml-1). Whereas compounds such as catechol, linalool, and sitosterol contributed to the high anticancer activities of this ethyl acetate extract.Key words: anticancer activity, HeLa cell lines, Raji cell lines, Toona sureni, Vero cell lines

Pengaruh Metode Ekstraksi Terhadap Kandungan Senyawa Polifenol dan Aktivitas Antioksidan pada Rubus fraxinifolius Yesi Desmiaty; Berna Elya; Fadlina Chany Saputri; Iis Irawatty Dewi; Muhammad Hanafi
JURNAL ILMU KEFARMASIAN INDONESIA Vol 17 No 2 (2019): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Rubus fraxinifolius can be founded in the mountainous regions of West Java. Previous studies have shown that this plant has potent antioxidant activity. The content of polyphenol compounds in this species contributes substantially to antioxidant activity, and the extraction method will affect the content of the compound. In this study an evaluation of the extraction method effect on leaves, young fruits and stems of R. fraxinifolius on the content of polyphenols and their antioxidant activity, have been done. Method: The leaves, fruits, and stems of R. fraxinifolius were extracted by maceration, reflux, and soxhlet using methanol as a solvent. Each extract was determined the total polyphenol content using Folin-Ciocalteu reagent and antioxidant activity test using DPPH reduction method. Result: The highest extract yield was R. fraxinifolius leaf reflux extract (30.20%). The highest total polyphenol content was given by the Soxhlet extract of leaf R. fraxinifolius (48.79 mg GAE / g extract). The highest antioxidant activity was leaf Soxhlet extract of R. fraxinifolius (98.29% at 100 ppm). Conclusion: From this study it was shown that the methanol extract of R. fraxinifolius leaves extracted by Soxhlet gave the highest polyphenol content and antioxidant activity.

ELUSIDASI STRUKTUR MOLEKUL UK-3, UK-4 DAN UK-5, SENYAWA BIOAKTIF DARI Streptomyces sp. 517-02. Muhammad Hanafi
Jurnal Kimia Terapan Indonesia Vol 8, No 1-2 (1998)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jkti.v8i1-2.204

New potential antifungal antibiotics UK-2A, B, C and D were elucidated as nine membered dilactone derivatives, isolated from mycelial cake of an actinomycete strain 517-02. In the continuation of a screening program in order to discover other useful bioactive metabolites from the same microbial sources, a novel UK-3 and two known compounds of UK-4 and UK-5 have been isolated.The molecular structure of UK-3 was very similar to UK-2A, except thai UK-3 did not have any methoxy group (-OCH3) on pyridine at ('-4'. UK-4 and UK-5 were identified as kown compounds of isocoumarine. These structures were elucidated based on their spectral and chemical evidence.Biological activity assay, demonstrated that UK-3 was active as against to Saccharomyces cerevisiae, Aspergillus niger dan Neurosporo sitophila and inhibited growth of cancer cell, P-388, B-16, KB and COLO-201.

The Synthesis of Quinidine Salicylate Ester Compound Intan Nurjaya; Muhammad Hanafi; Puspa D.N Lotulung; Teni Ernawati; Sri Mursiti
Jurnal Kimia Terapan Indonesia Vol 20, No 2 (2018)
Publisher : Research Center for Chemistry - LIPI

Quinidine a compound isolated from quinine plants, one of the species of quinine plants is (Chincona ledgereriana) From PT SIL Lembang. The purpose of this study was obtain quinidine salicylate ester through esterification reaction. In this study, the synthesis of quinidine ester compound by esterification reaction was conducted. Esterification reaction was conducted by using DCC activator and DMAP catalyst with one carboxylic acid namely salicylate acid producing new compound namely quinidine salicylate, Subsequent Quinidine salicylate was obtained in the form of oil with 97% yield. The compound obtained from the synthesis was then identified using Thin Layer Chromatography continue analyzed using with Spectrophotometer, LC-ESI-MS spectroscopy. Results show that the target compound has been successfully synthesized.

ISOLASI DAN PENENTUAN STRUKTUR MOLEKUL DARI TANA MAN OBAT TRADISIONAL SMILAX CORDIFORIA Muhammad Hanafi
Jurnal Kimia Terapan Indonesia Vol 6, No 1-2 (1996)
Publisher : Research Center for Chemistry - LIPI

A research was carried out to isolate the constituents of Smilax cordiforia trunk bark. It is a famous medicinal plant in Mexico, used as weight control. The isolation methods were carried out by maceration in ether, and remaceration in methanol, and then both of crude extracts were purified by using combined chromatography technique. In the purification and identitification of the ether extract two compounds as steroid saponins, 3-B-O -B-D-glucopyranosyl sitosterol (1) and a mixture of 6-O-palmitoyl-glucopiranosil sitosterot, 6-0stearylglucopyranosyl sitosterol and 6-0arachidoyl-glucopyranosyl sitosterol (2) were obtained, which also have been isolated from other medicinal plants. Isolation from methanol extract resulted in four known substance of phenolic derivatives, identified as afilbin (3) isoastilbin (4), engelitin (5). and chlorogenic acid (C). engelitin demonstrated antifungal activity, astilbin and chlorogenic acid showed antifungal, antibacterial, and antiviral activities. Chlorogenic acid also exhibited antimutagenic, antitumour and antioxcidant activities. Their molecular structures were detemined on the basis of combied spectroscopic methods including two dimensional NMR (2D NMR).

ISOLATION AND STRUCTURE ELUCIDATION OF A NEW OLEANOLIC ACID AND ITS SAPONINS FROM Thylacospermum caespitosum Muhammad Hanafi
Jurnal Kimia Terapan Indonesia Vol 7, No 1-2 (1997)
Publisher : Research Center for Chemistry - LIPI

A new oleanollc acid and its saponins have been isolated from methanol and ether extract of the whole plant of cushion plant T. caespltosum. The compounds were established as olean-12-en-3b-16a-diol-23,28-dioic acid (1), 3jJ-O-jJ-D-glucopyranosylolean12-en-16a-diol-23-doic acid (2), and 28-O-B-D-glucopyranosylolean--12-en3b, 16a-diol-23-dioc acid (3), which are trivial named as thylacospermic acid (1), thylacospermicoside A (2) and thylacospermicoside B (3), respectively. Three known steroids namely 22,23-dihydrospinasterol (4), a-spinasterol (5), and 3jJ-OjJ-D-glucopyranosylspinasterol(6) [lJ and the mixture of phytoceramides (7) [2, 8] were also isolated from the ether extract. The compounds were identified based on chemical and spectroscopic methods. This is the first report on the isolation of the compounds from genus Thylacospermum.

SKRINING IN SILICO POTENSI SENYAWA ALLICIN DARI ALLIUM SATIVUM SEBAGAI ANTIPLASMODIUM Fatmawaty Fatmawaty; Muhammad Hanafi; Rosmalena Rosmalena; Vivitri Dewi Prasasty
Jurnal Kimia Terapan Indonesia Vol 17, No 2 (2015)
Publisher : Research Center for Chemistry - LIPI

Allicin compound (2-propene-1- sulphinothioat acid S-2-propenyl ester) is known to have potential as antiplasmodium in vitro. However, the inhibitory activity mechanism of Allicin to plasmodium is still unknown. In this research, we determined the inhibitory activity of Allicin in silico. Identification of physicochemical properties of Allicin compound and two Allicin derivatives, Alc1, Alc2 and Ac2Alc3 were also conducted.. Furthermore, analysis of drug-likeness and adsorption-distribution-metabolism-excretion (ADME) were carried out on the Allicin compound and its derivatives to find the potential of these compounds as drug candidates. In determining the specific interaction, we utilized molecular docking analysis between Allicin and its derivatives against a protein target Cysteine Protease (SP). MolecularÂ docking results showed that Allicin derivative, Alc2 (S-prop-2-en-1-yl 3-methylbut-2-ene-1-sulfinothioate, C10H18OS2) has better potential as inhibitors than Allicin, based on the lower bond energies and the inhibition constants, thus Alc2 can be used as an antiplasmodium agent candidate.Keywords: Allicin, Allicin derivatives, drug likeness, ADME, molecular docking

Identification and Characterization of Bacterial Lipase from Plateu Soil in West Java Vivitri Dewi Prasasty; Vinella Winata; Muhammad Hanafi
Jurnal Kimia Terapan Indonesia Vol 18, No 02 (2016)
Publisher : Research Center for Chemistry - LIPI

Lipases are known as glycerol ester hydrolases that catalyze the hydrolysis of triglycerides into free fatty acids and glycerol. Lipases are found in human, animal, plant, and microorganisms. The aim of this research is to identify lipase producers and characterize bacterial lipase from West Java plateau soil. Plateau soil bacteria samples were isolated on lipase screening medium containing Rhodamine B. Olive oil was used as a substrate in screening and production medium bacterial lipases. From 16 bacterial isolate of lipase producers, 14 were identified as Bacillus sp. and the others were identified as Pseudomonas alcaligenes. All isolates were taken into production step to determine their lipase activities. Moreover, top 3 lipase activities out of 16 lipase activities were chosen to find the optimum pH and temperature. Both characterizations showed pH optimum and temperature optimum from each lipase. These optimum condition were used in heat stability characterization for each lipase samples. The result showed that lipase from isolate COK 2 in optimum pH 4 and temperature 50oC was the most stable lipase due to this sample has good and stable activity for 1 to 5 hours incubation time. Lipase sample from isolate COK 2 has good efficiency for lipase productivity in acid condition and high temperature. Results of this investigation could encourage utilization of these activity enhancers for various industrial applications.

ISOLATION AND STRUCTURE ELUCIDATION OF THE CUSHION PLANT POTENTILLA ARTICULATA Muhammad Hanafi; Shibata Kozo; Mamoru Kanzaki
Jurnal Kimia Terapan Indonesia Vol 6, No 1-2 (1996)
Publisher : Research Center for Chemistry - LIPI

Methanol extract of the whole plant of P. articulata Franch was fractionated by using open column silica gel chromatography following identification, resulted in the isolation of known phytoceramides, N-(2'-hydroxy-acyl)-2-amino1,3,4trihydroxy-8-octadecene) (1), which also isolated from Urtioca dioca and Thylacospermum caespitosum, and two known steroids, identified as ~-sitosterol (2), 3B-O-B-D-glucopyranosylsitosterol (3), which have also been isolated from Prunella vulgaris L. var. Iilacina (Labiatae). Triterpenoic acids were elucidated as 2B,19a-dihydroxyursolic acid (4), a mixture of Zcc-monohydroxyursclic acid (5) and 2a-monohydroxyoleanolic acid (6), also found in Geurn japonicum Thunberg, 19a-monohydro-xyursolic acid (7), a mixture ursolic acid (8) and oleanolic acid (9), which were also obtained in Isodon japonicus Hara. Their structure identifi-cations were based on chemical and spectroscopic methods.

Co-Authors . Hariyanti Akhmad Darmawan Andi Sadapotto Andi Sadapotto Andri Eko Putra Atiek Soemiati Atiek Sumiati B. Suwandl Berna Elya D Efrina Dadan Ridwanuloh Endang Saepudin, Endang Evawani Elysa Lubis Fadlina Chany Saputri Fatmawaty Fatmawaty Fauzi Febrianto Hasan Hasan Herry Cahyana Ibrahim manda Iis Irawatty Dewi Ika Mustika Ilhamuddin Ilhamuddin Intan Nurjaya Kurnia Sofyan Lababa Lababa Lotulung, Puspa Dewi N. Mamoru Kanzaki Megawati, Ngadiman Ngadiman Nina Artanti Nova Yohana Nurmala Nurmala Nurmala Nurmala Nurul Fadhilah Suardi Puspa D.N Lotulung Renhart Jemi Rina Hidayati Pratiwi Rita K Sari Rita Kartika Sari Rosa Dewi Pratiwi Rosmalena Rosmalena Sam Hermansyah Santy Rahmawati Shibata Kozo Sofa Fajriah Soleh Kosela Sri Mursiti Suminar A Achmadi Suminar S Achmadi Suminar S Achmadi Syahrul Aiman Syamsu T Teni Ernawati Usman M Vinella Winata Vivitri Dewi Prasasty Wasrin Syafii dan Kurnia Sofyan Gustan Pari Buchari Yanotama T Laksana Yasir ", Yasir Yesi Desmiaty, Yesi

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