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Detection of Antibodies Brucella abortus in dairy cattle in Puspo District, Pasuruan using Rose Bengal Test and Complement Fixation Test Agung Jati Kusuma; Erma Safitri; Ratih Novita Praja; Wiwiek Tyasningsih; Maya Nurwartanti Yunita; Prima Ayu Wibawati
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.199-206

Abstract

Brucellosis was an infectious disease caused by the genus brucella. Brucellosis in dairy cattle was caused by Brucella abortus that impaction an abortion. The aim of this study was to determine the presence of antibodies of the Brucella abortus in adult female dairy cattle in the Puspo district Pasuruan using Rose Bengal Test (RBT) and Complement Fixation Test (CFT). A descriptive analysis method with quantitative approach was used in this study. Meanwhile, interviews were conducted to get supporting information from the farmer. Used 100 samples. The results of the study showed 2% samples considered positive brucellosis tested by RBT. To confidence false-positive result, RBT test was followed by CFT. After tested by CFT, in this study there were no brucella antibodies in adult female dairy cattle in Puspo district, Pasuruan.
Most Probable Number of Escherichia Coli in Fresh Milk at KPSP Ijen Makmur, Licin Sub-District, Banyuwangi Achmad Arby Wijaya; Iwan Sahrial Hamid; Maya Nurwartanti Yunita; Wiwiek Tyasningsih; Ratih Novita Praja
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.207-212

Abstract

The study aimed to identify the total number of E. coli used in fresh cow milk in KPSP Ijen Makmur. The sample was used by as many as 16 samples from a group of cattle farmers. E. coli analyzed using Most Probable Number (MPN), 3 series of tubes. Before continuing the MPN test the milk must be diluted, 25 ml of milk was poured into the dilution of the 225ml peptone water buffered then homogenized for 2 minutes. MPN consist of presumptive coliform test if positive was found of gas and was cloudy, presumptive faecal coliform test positive was found in the gas and was cloudy, confirmed E.coli test if there was a black colony with or metallic green, continued by a biochemical test with red ring-positive Indole, Methyl Red positive the color is red, negative Voges Paskauer if there is no change in color, and negative citrate will turn green. Results showed that from the 16 samples of fresh milk used there were 7 samples of fresh milk that exceeded the contamination limit E. coli or < 3 apm /ml. Conclusion, number of E. coli in fresh milk at KPSP Ijen Makmur 43.75% of the total sample exceeded maximum contamination.
Necrosis Description of Mice Liver Induced with Monosodium Glutamate and Methanol Robusta Coffee Bean Extract (Coffea canephora) Tisya Yumn Al-Zuhroh; Kuncoro Puguh Santoso; Maya Nurwartanti Yunita; Nove Hidajati; Ratih Novita Praja
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.213-220

Abstract

This study aimed to analyze the effect of methanol robusta coffee bean extract (Coffea canephora) on liver histopathological description in mice induced with monosodium glutamate (MSG). This study was used 20 male mice for 5 treatment groups: K + (MSG 0.12 mg and Vitamin C 6 mg), K- (MSG 0.12 mg and CMC Na 0.1 ml), P1 (MSG 0.12 mg and RCE 0.1 mg), P2 (MSG 0.12 mg and RCE 0.2 mg), and P3 (MSG 0.12 mg and RCE 0.4 mg) per orally for 42 days. Liver histological scores were analyzed using Kruskal Wallis test. The results of Kruskal Wallis test scored p=0,391 which indicates that the data is not significant (p>0.05). In the control and treatment groups there were no significant. In conclusion, the giving methanol robusta coffee bean extract did not show a decrease in hepatosite damage that was different from the negative control treatment group.
The Risk Factor of Subclinical Mastitis Incident in Dairy Cattle in KPSP Ijen Makmur, Banyuwangi Indah Puspita Ningrum; Soeharsono Soeharsono; Prima Ayu Wibawati; Yeni Dhamayanti; Maya Nurwartanti Yunita
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.48-53

Abstract

Mastitis is an inflammatory disease in the internal tissue of the mammary gland. Subclinical mastitis does not show any changes in the shape of the mammary gland and to detect it can be examined the number of somatic cells in milk. Subclinical mastitis uses the California Mastitis Test (CMT) method as preliminary research to obtain preliminary data on the incidence of subclinical mastitis in KPSP Ijen Makmur, Licin District, Banyuwangi Regency. This research is descriptive research. The detection for this research was carried out by taking milk samples in cows per purposive and reacted CMT reagents issued circularly for 10-15 seconds. Observation of results is determined based on changes inconsistency with the existence of gel and the results of interview farmers as supporting data. The results showed that the influencing factors incidence of subclinical mastitis in KPSP Ijen Makmur, Licin District, Banyuwangi Regency were milk production, age and amount of lactation. In contrast, the biggest factor for reducing the risk of subclinical mastitis is udder health followed by body conditions measured by Body Condition Score.
Risk Factors and Scabious Prevalence in Cats During 2020 in Griya Satwa Clinic, Magetan Nedriana Cahya; Hardany Primarizky; Maya Nurwartanti Yunita
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.81-86

Abstract

Scabious is a disease that often infests cats, originating from the family Sarcoptidae. This study aimed to determine the prevalence and factors that can affect the risk of scabious in cats. The study was evaluated at the Griya Satwa Clinic, Magetan in January-February 2021. The design of this study was a retrospective cohort study by taking secondary data during the 2020 period as study material. The data obtained were then entered into the Statistical Package for the Social Sciences (SPSS) version 20 for windows to analyze the association's relationship and increased risk by calculating the Chi-Square and Odds Ratio. The samples obtained were 76 cats infested with scabious. The analysis of the association of risk factors for scabious was breed (P = 0.428), age (P = 0.793), sex (P = 0.426). The results of the prevalence of scabious in cats from the study during January-February 2021 in the 2020 period were 9.5%, and the analysis of the association (Chi-Square) and the increased (Odds Ratio) of factors including breed, age, sex on the risk of scabious in cats were not significance / absent influence, The probability of error obtained is outside the tolerance set by the researcher, so the results are not significant (p>0,05).
Molecular Identification of Pathogenic Leptospirosis with Zoonotic Potential in Urban Rats (Rattus Rattus) in Banyuwangi District Maya Nurwartanti Yunita; Bodhi Agustono; Muhammad Thohawi Elziyad Purnama
Bantara Journal of Animal Science Vol 2, No 1 (2020)
Publisher : Program Studi Peternakan, Fakultas Pertanian, Universitas Veteran Bangun Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32585/bjas.v2i1.639

Abstract

Leptospirosis is a zoonotic disease that occurs globally. Leptospires are transmitted through the urine of infected mice, through injured skin or mucosal invasion. Infection can occur through direct contact or through contact with contaminated water or soil. This qualitative research uses a combination of epidemiological studies with a case study approach. Rat urine metabolism samples will be tested by testing the base pair chain through the Polymerase Chain Reaction method for leptospirosis. Electrophoresis results showed 5% percent of positive samples with 330 bp. The conclusion of this study, the molecular identification of leptospirosis from rat urine using the Polymerase Chain Reaction technique are found positive results so prevention is needed to control the distribution of pathogenic leptospirosis bacteria. Urban rats (Rattus rattus) in leptospirosis transmission are considered as the most important leptospira reservoir, because high population densities of this species can cause an increase in the spread of leptospirosis. Keywords: Banyuwangi, Leptospirosis, Urban rats, Zoonosis
Gambaran Histologi Lebar Vili Jejunum Kelinci Jenis Rex dengan Subtitusi Bahan Penyusun Tepung Ikan dengan Tepung Teritip (Cirripedia sp.) Maya Nurwartanti Yunita; Bodhi Agustono; Amung Logam Saputro
Jurnal Nutrisi Ternak Tropis Vol 3, No 1 (2020): JNT | Jurnal Nutrisi Ternak Tropis Maret 2020
Publisher : Jurnal Nutrisi Ternak Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jnt.2020.003.01.6

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh tepung teritip (Cirripedia sp.) Sebagai pakan pengganti dari tepung ikan pada pakan kelinci, seperti yang diamati dari lebar jejunum villi. Kelinci sebanyak 100 ekor ditempatkan pada kandang individu dengan pakan standart yang sesuai kebutuhan mereka secara ad libitum. Persentase feed substitusi dengan tepung teritip dengan empat perlakuan dan lima replikasi dalam pakan sebesar 0%, 2,5%, 5%, 7,5% pada masing-masing kelompok. Perlakuan dilakukan selama 4 minggu, data dikumpulkan setelah pengorbanan akhir perlakuan. Data akhir penelitian ini dianalisis menggunakan analisis variance dengan angka tingkat signifikansi 0,05. Hasil analisis data menunjukkan bahwa tidak ada signifikansi (p> 0,05) pada lebar vili jejunum. Penggunaan tepung teritip sebagai pengganti pakan untuk tepung ikan pada kelinci. Pakan lengkap berpengaruh signifikan terhadap lebar vili jejunum.
Pengaruh Subtitusi Tepung Ikan Dengan Tepung Bernacle Terhadap Berat Organ Pencernaan dan Lemak Abdominal Kelinci Pedaging Jantan (Orytolagus cuniculus) Bodhi Agustono; Maya Nurwartanti Yunita; Sunaryo Hadi Warsito; Widya Paramita Lokapirnasari; Mohammad Anam Al Arif; Ragil Angga Prastiya; Amung Logam Saputro
Jurnal Nutrisi Ternak Tropis Vol 3, No 1 (2020): JNT | Jurnal Nutrisi Ternak Tropis Maret 2020
Publisher : Jurnal Nutrisi Ternak Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jnt.2020.003.01.8

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh tepung teritip (Cirripedia sp.) Sebagai pengganti pakan dari tepung ikan pada pakan kelinci, seperti yang diamati dari lemak abdominal dan berat organ pencernaan kelinci jantan rex. Kelinci sebanyak 100 ekor ditempatkan pada kandang individu dengan pakan standart yang sesuai kebutuhan mereka secara ad libitum. Persentase feed substitusi dengan tepung teritip dengan empat perlakuan dan lima replikasi dalam pakan P0 (TI 15% dan TT 0%), P1 (TI 12,5% dan TT 2,5%), P2 (TI 10% dan TT 5%), P3 (TI 7,5% dan TT 7,5%). Perlakuan dilakukan selama 4 minggu, data dikumpulkan setelah pengorbanan akhir perlakuan. Usus kecil kelinci dikumpulkan dan menimbang organ pencernaan termasuk lambung, usus kecil dan usus besar. Hasil penelitian dianalisis menggunakan analisis varians dengan tingkat signifikansi 0,05. Berdasarkan hasil analisis menunjukkan bahwa tidak ada perbedaan yang signifikan (p> 0,05) pada lemak perut. Hasil penelitian menunjukkan tidak ada perbedaan yang signifikan (p> 0,05) dalam berat organ pencernaan (lambung, usus kecil, dan usus besar). Tepung teritip dapat digunakan sebagai pakan pengganti tepung ikan dalam pakan lengkap untuk kelinci jenis rex hingga 7,5%.
MOLECULAR DETECTION OF GENE RESISTANT TO VARIOUS INSECTICIDES IN Aedes aegypti AT BANYUWANGI EAST JAVA USING POLYMERASE CHAIN REACTION Aditya Yudhana; Ratih Novita Praja; Maya Nurwartanti Yunita
Jurnal Kedokteran Hewan Vol 12, No 2 (2018): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (175.022 KB) | DOI: 10.21157/j.ked.hewan.v12i1.7625

Abstract

The purpose of this study was to determine the type of insecticides which Aedes aegypti mosquitoes are resistant to, so that other susceptibleinsecticides still can be used. The study was a cross sectional epidemiological study with cluster sampling in Sub-district Wongsorejo,Banyuwangi, Muncar, Tegaldlimo, Kalibaru which is considered sufficient to represent Banyuwangi Regency. Mosquito samples appropriate tothe characteristics were isolated and tested for insecticide-resistance primer namely voltage gated sodium channel (VGSC) to determine thespecific resistance expressed in mosquitoes. The result of resistance test using WHO standard method showed that Aedes aegypti mosquito fromBanyuwangi Regency were resistant to cypermethrin 0.25% and malathion 0.8%. There was a VGSC coding gene with 250 bp band detectedusing polymerase chain reaction (PCR) technique which was associated with the resistance of Aedes aegypti mosquitoes in Banyuwangi Regencyto organophosphate insecticides (malathione) and pyrethroid insecticides (cypermethrin).
MOLECULAR DETECTION OF GENE RESISTANT TO VARIOUS INSECTICIDES IN Aedes aegypti AT BANYUWANGI EAST JAVA USING POLYMERASE CHAIN REACTION Aditya Yudhana; Ratih Novita Praja; Maya Nurwartanti Yunita
Jurnal Kedokteran Hewan Vol 12, No 2 (2018): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i2.7625

Abstract

The purpose of this study was to determine the type of insecticides which Aedes aegypti mosquitoes are resistant to, so that other susceptibleinsecticides still can be used. The study was a cross sectional epidemiological study with cluster sampling in Sub-district Wongsorejo,Banyuwangi, Muncar, Tegaldlimo, Kalibaru which is considered sufficient to represent Banyuwangi Regency. Mosquito samples appropriate tothe characteristics were isolated and tested for insecticide-resistance primer namely voltage gated sodium channel (VGSC) to determine thespecific resistance expressed in mosquitoes. The result of resistance test using WHO standard method showed that Aedes aegypti mosquito fromBanyuwangi Regency were resistant to cypermethrin 0.25% and malathion 0.8%. There was a VGSC coding gene with 250 bp band detectedusing polymerase chain reaction (PCR) technique which was associated with the resistance of Aedes aegypti mosquitoes in Banyuwangi Regencyto organophosphate insecticides (malathione) and pyrethroid insecticides (cypermethrin).