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Journal : ACTA VETERINARIA INDONESIANA

Phenotypic and Genotypic Study of Antibiotics Resistance Profile in Escherichia coli Isolated from Broilers in Cianjur, Indonesia Aprilia Hardiati; Safika; I Wayan Teguh Wibawan; Fachriyan Hasmi Pasaribu
Acta VETERINARIA Indonesiana Vol. 9 No. 2 (2021): Juli 2021
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.9.2.97-104

Abstract

This study aimed to investigate the phenotypic and genotypic of antibiotics resistance profile in Escherichia coli. The 30 samples come from cloacal swab of broilers in Cianjur, Indonesia. Isolation and identification of E. coli was performed by culture in McConkey agar, eosin methylene blue agar, Gram staining and five essential biochemical tests (IMViC). In this study, 10 isolates (33.3%) were confirmed E. coli positive. Phenotypic profile was performed by screening all isolates with 8 antibiotics of 6 antibiotic groups. The screening was carried by Kirby-Bauer disk diffusion method based on the standard of CLSI. For genotypic profile, each resistant isolate was detected antibiotic resistance-encoding gene. The result showed all isolates (100%) resistant against tetracyclin, oxytetracycline and erythromycin. Nine isolates (90%) detected nalidixic acid and enrofloxacin-resistant. The ciprofloxacin and gentamicin-resistant isolates were 70% and 40%, respectively. There was no resistant isolate for chloramphenicol. Multi drug-resistant was detected on 90% isolates. Only gyrA (100%) and tetA (80%) genes were detected. This study showed high rate of occurrence of antibiotic resistance in E. coli. Not all resistant isolates were detected in the antibiotic resistance-encoding gene in this study. Future research to detect resistance genes should use more varied target genes.
Kelimpahan Fungi Kelas Dothideomycetes Pada Lumba-lumba Hidung Botol (Tursiops aduncus) Kurnia Tiara Aulia; Agustin Indrawati; Safika
Acta VETERINARIA Indonesiana Vol. 10 No. 2 (2022): Juli 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.2.148-156

Abstract

Mamalia laut memainkan peran ekologis penting di lautan, dan menjadi prioritas global karena kepekaan terhadap perubahan lingkungan. Lumba-lumba hidung botol (Tursiops aduncus) merupakan salah satu mamalia laut yang tersebar luas di Indonesia. Informasi mengenai mikrobioma masih sangat sedikit diketahui, terutama informasi mengenai mikrobioma fungi. Penelitian ini dilakukan untuk mengidentifikasi kelimpahan fungi kelas Dothideomycetes pada saluran pencernaan khususnya usus lumba-lumba hidung botol (Tursiops aduncus) dengan menggunakan Platform Next Generation Sequencing (NGS). Penelitian ini berhasil mengidentifikasi 6 genera dari 4 ordo yang yang meliputi Neodevriesia, Altenaria, Stemphylium, Phaeophleospora, Diplodia dan Venturia. Kelompok Neodevriesia adalah yang paling mendominasi sebesar 62%, diikuti Alternaria 16% dan Phaeophleospora 14%. Fungi yang tidak teridentifikasi mencapai 5%. Kelimpahan fungi pada usus lumba-lumba hidung botol (Tursiops aduncus) diasumsikan memiliki korelasi dengan lingkungan dan makanan lumba-lumba pada pusat konservasi.