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All Journal Hemera Zoa Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga. Farmasains : Jurnal Farmasi dan Ilmu Kesehatan COPING (Community of Publishing in Nursing) Jurnal Akademika Biologi Jurnal Ilmu Komputer dan Agri-Informatika Jurnal Online Mahasiswa (JOM) Bidang Ilmu Sosial dan Ilmu Politik Jurnal Online Mahasiswa (JOM) Bidang Ilmu Hukum Indonesian Journal of Pharmaceutical Science and Technology JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Journal of Agromedicine and Medical Sciences I-ECONOMICS: A Research Journal on Islamic Economics AlphaMath: Journal of Mathematics Education JURNAL PENDIDIKAN TEMATIK DIKDAS Al-Mustashfa JNKI (Jurnal Ners dan Kebidanan Indonesia) (Indonesian Journal of Nursing and Midwifery) Argipa (Arsip gizi dan Pangan) BASIS (BAHASA DAN SASTRA INGGRIS) INSPIRAMATIKA Jurnal Penelitian Sosial dan Ekonomi Kehutanan Al-Madrasah: Jurnal Ilmiah Pendidikan Madrasah Ibtidaiyah Jurnal Ilmiah Keperawatan (Scientific Journal of Nursing) PROSIDING SEMINAR NASIONAL PROGRAM PASCASARJANA PARAMUROBI Faletehan Health Journal Bioscientia Medicina : Journal of Biomedicine and Translational Research de Fermat : Jurnal Pendidikan Matematika Abdimas Universal Keberlanjutan : Jurnal Manajemen dan Jurnal Akuntansi Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal Jurnal Metaedukasi : Jurnal Ilmiah Pendidikan Journal of Classroom Action Research Tahdzib Al-Akhlaq: Jurnal Pendidikan Islam Indonesian Journal of Sociology, Education and Development (IJSED) Indian Journal of Forensic Medicine & Toxicology Edulead : Journal of Education Management Bioscientia Medicina : Journal of Biomedicine and Translational Research JCA (Jurnal Cendekia Akuntansi) Proceeding of Mulawarman Pharmaceuticals Conferences Jurnal Penyuluhan Agama Obstretika Scientia Pontianak Nutrition Journal (PNJ) Journal of Educational Engineering and Environment Jurnal Penelitian Sains dan Kesehatan Avicenna Jurnal Ilmiah Kesehatan Keperawatan
HUSNUL KHOTIMAH
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Journal : Farmasains : Jurnal Farmasi dan Ilmu Kesehatan

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PENGARUH POLIFENOL TEH HIJAU TERHADAP PRODUKSI TNF-A (TUMOUR NECROSIS FACTOR-A) PADA KULTUR SEL TROFOBLAS MANUSIA YANG DIPAPAR GLUKOSA TINGGI 33 Mm Siti Aisah; Sasmito Djati; Husnul Khotimah
Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Vol. 1 No. 1 (2010): April-September 2010
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/far.v1i1.425

Abstract

The purpose of this research was to study the effect of green tea polyphenol to TNF-á production on human trophoblast cell culture exposed by 33 mM glucose. Trophoblast culture isolated from human fetal placental tissue by sectio caessaria. Monolayer trophoblast cells that had been incubated for 3 days at 5% CO2; 37°C were divided into 2 groups: (1) normal glucose (5 mM) and (2) glucose 33 mM exposure, both divided into 2 sub groups: (a) without green tea polyphenol treatment, and (b) green tea polyphenol treatment 0,1; 0,2; and 0;4 mg/ml. Cells incubated for 3 days at 5% CO2; 37°C then analyzed cytotrophoblast cells characteristic. TNF-á level was measured by ELISA and analyzed with oneway ANOVA. Immunocytochemistry showed the number of cells that expressed TNF-á then analyzed descriptively. The results of this study showed that TNF-á level at 0,1; 0,2; and 0,4 mg/ml polyphenol were 2804,333 ñg/mL; 2513,222 ñg/ml; and 2739,889 ñg/ml respectively compared with 2739,889 ñg/mL normal glucose without polyphenol, and 2721,000 ñg/mL; 2612,111 ñg/mL; and 2566,555 ñg/mL compared with 2621,000 ñg/mL glucose 33 mM exposure without polyphenol. Number of cells that expressed TNF-á with 0,1; 0,2; and 0,4 mg/ml polyphenol treatment were 0%; 2%; and 5,5% compared with 0% normal glucose without polyphenol, and 82%; 0%; and 0% compared with 3% glucose 33 mM exposure without polyphenol. Green tea polyphenol exposure for 3 days at 0,1; 0,2; and 0,4 mg/ml didn’t significantly affect the decreasing of TNF-á production. Keywords: GDM, green tea, polyphenol, TNF-á, trophoblast
Co-Authors A. Otong Busthomi Abdul Hamid Wahid Ahmad Kholid Fauzi Amrullah Anak Agung Gede Sugianthara Anas Muhktar Andita Wahyuningtyas Anggaunita Kiranantika Anna Muawanah Aswati Aswati Ayuni Arfina Bagus Supriyadi Bakti Tamba Bambang Firmansyah Bambang Yulianto Dedi Ansori Desi Apriani Dhani Yuniati Duwi Lismawati Dwi Adi Nugroho Eka Yuli Astuti Elisa Nurul Laili Erizka Rivani Ester G Pansserga Evin Andriani Fatmawati Ferawati Ferawati Fitnaningsih Endang Cahyawati Fitriani Fitriani Gatut Rubiono Habibur Rahman Hadiyanto Harianto Budi Rahardjo Hary Soedarto Harjono Hasanuddin " HESTI ATASASIH I Ketut Karya Wisana Iin Sumarto Iis Iis Iis Trisnawati Ima Mulyawati Imas Sukaesih Sitanggang Indah Nur Chomsy Indhra Musthofa Irma Yeny Irman Hermadi Juju Jumena Kadar Kuswandi Khoirus Sachiyah Kholisotin, Kholisotin Kirnantoro Kirnantoro Lubna Maimunah M. Saifur Rohman Mifetika Lukitasari Mirhansyah Ardana Moch Sasmito Djati Mohammad Irwansyah MUHAMMAD AGIL Mukhlis R Nadiyah Khairiyah Aziz Nanda Wahyu Anandita Novida Ariani Nur Amin Nur Samsu Nurul Badriyah Haqiqiyah Nyoman Sridana RACHMAT HIDAYAT Rahayu Sri Waskitoningtyas Rahman, Handono Fatkhur Ratna Yulis Tyaningsih Refi Lindawati Rejeki Siti Ferniah Ririn Maghfirah Risna Agustina Rizky Hafifatul Umam Roziana Roziana Santi Sundary Lintang Shanti Meris Dwi Astuti SITI AISAH Siti Durrotul Mukarromah Siti Nur Jannah Sri Astutik Andayani SRI UTAMI SUBAGIO Suci Yuniarti Sulfiati Ira Wijayanti Susy Katarina Sianturi Sutrisnak Sutrisnak Teguh Wahyono Tingga Sari Novi Prakaswati Tri Hariyati Nur Indah Sari Tuty L Yusuf Widhi Kurniawan Yani Nurhadryani YESSI ALZA Yuliono Trika Nur Hasan Zahrotul Wathoniyah,