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Journal : Biosaintifika: Journal of Biology

Raw Secondary Metabolites of Trichoderma harzianum T10 in Tapioca Flour Towards Cucumber Damping-off Soesanto, Loekas; Ilahiyyah, Hidayatul; Mugiastuti, Endang; Manan, Abdul; Rostaman, Rostaman
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.24567

Abstract

Trichoderma harzianum is effective for controlling soil-borne pathogenic fungi and producing secondary metabolites. When applied in the field, the raw secondary metabolites are quickly decreased directly by sunlight. One strategy to avoid degradation is the use of tapioca fluor liquid formula for biological control agents. This research aimed to obtain the most effective concentration of tapioca flour in development of raw secondary metabolites of Trichoderma harzianum T10, its effect on damping-off and growth of cucumber. This research was carried out at the screen house and the Plant Protection Laboratory, Faculty of Agriculture, Jenderal Soedirman University from September 2017 up to January 2018. The study was conducted in two stages, i.e., in vitro and in planta. The in vitro stage used completely randomized design with five repetitions and five treatments consisted of T. harzianum T10 in Potato Dextrose Broth, and in 0.5; 1; 1.5; and 2% of tapioca flour media. In in planta, randomized block design was used with five repetitions and six treatments consisted of control, T. harzianum T10 in PDB, and in 0.5; 1; 1.5, and 2% of tapioca flour media. Variables observed were density of conidia, disease incubation period, disease incidence, AUDPC, maximum growth potential, germination ability, plant height, canopy fresh weight, root length, and fresh root weight. Result of the research showed that the highest conidial density (1.23 x 107 conidia mL-1) of T. harzianum T10 was found in 2% tapioca flour with an increase of 63.28% compared to the PDB. The tapioca flour of 1 and 2%, and PDB could suppress the disease incidence by 81.82%. The lowest AUDPC was at 2% tapioca flour. The raw secondary metabolites could not delay the incubation period significantly and increase cucumber plant growth. The novelty is the use of antagonistic fungi in terms of raw secondary metabolites and the discovery of tapioca flour with the right concentration to produce high conidia density and high raw secondary metabolites. The benefits are to find other cheaper ingredients in promoting antagonistic fungal growth and the use of antagonistic fungal bioactive compounds to control plant pathogen
Co-Authors A.A. Ketut Agung Cahyawan W Abd. Razak Abdul Hadi Abdul Hadi Abdul Hadi Abdul Karim Agus Dana Permana dan Rostaman ., Agus Dana Permana dan Rostaman Ahmad Nalhadi, Ahmad Ahmad Zaki Husaini Al Yasa Abubakar Amiruddin Amiruddin Amiruddin Amiruddin Andi Safrizal Andik Kurniawan Anwar Daud Asnawi Muslem Asyura Bayu Kurniawan Cecep Kusmana Cut Intan Salasiyah Cut Putroe Yuliana Dara Yusnida DARINI SRI UTAMI Dei, Abdul Rajab Dicky Wirianto Dwi Budi Santoso Endang Mugiastuti Fauzi Ismail Firdanis Setyaning Handika Fyka, Samsul Alam Habiburrahim Habiburrahim Hakim, Nasruan Harianto Respati Harviyaddin, Harviyaddin Hidrawati, Hidrawati Husaini, Ahmad Zaki Ikhwan Ikhwan Ilahiyyah, Hidayatul Iping Rahmat Saputra Iwan Marwan, Iwan Jovial Pally Taran Kamarullah Kamarullah Khairi Parwita Khairisman Khairisman Khairisman, Khairisman Lailiyah, Hidayatul Laode Sabaruddin Loekas Soesanto Luthfi Auni Maftukhin Maftukhin Mahmud Hamundu, Mahmud Mahmudi, Hadi Misri A. Muchsin Mohammad Efendi, Mohammad Muchsin, Misri M. Muhammad Arif Fadhilah Muhammad Arifin Muhammad Arifin Muhammad Imron, Muhammad Muhazar Muhazar Muhazar, Muhazar Mukhsin Nyak Umar Mulia Mulia Mulia Mulia Musadar Mappasomba Mustain Mustain Muzakki Muzakki, Muzakki Nanda Ria Nasruan Hakim Nur Arafah Nuraini Mannan Prastyani, Noviana Pratama, Willy Dhika Rachman, Nurul Ristanti Rachmawati, Windasari Rahmad Syah Putra Rahmad Syah Putra Rahmad Syah Putra, Rahmad Syah Rahmad Syahputra Rahmat Syahputra Rasyad Rasyad Rita Safari Rizki Maulana Ruhamah Ruhamah Safuan, Laode Sahari, Sariamin Saifuddin A. Rasyid Sakhidin Sanusi Ismail Saprijal Saprijal Saprijal Saprijal Sari, Lintang Yunita Siti Habsari Pratiwi, Siti Sumiman Udu Syahputra, Rahmad T. Lembong Misbah Tathahira Tathahira