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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Penelitian Saintek ILMU KELAUTAN: Indonesian Journal of Marine Sciences Bioma : Berkala Ilmiah Biologi BERKALA FISIKA JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA Jurnal Natur Indonesia Jurnal Akademika Biologi Prosiding KPSDA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Indonesian Food and Nutrition Progress Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Media Farmasi Indonesia Jurnal Energi Baru dan Terbarukan AGRIC Open Access DRIVERset
Endang Kusdiyantini
Departemen Biologi Fakultas Sains Dan Matematika, Universitas Diponegoro, Semarang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences

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Aktivitas Antioksidan Berbagai Minyak Edible Menggunakan Metode DPPH Enny Yusuf Wachidah Yuniwarti; Tyas Rini Saraswati; Endang Kusdiyantini
Buletin Anatomi dan Fisiologi Volume 3, Nomor 1, Tahun 2018
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.3.1.2018.85-88

Abstract

Natural antioksidan secara tradisonal sudah digunakan untuk mencegah terjadinya penyakit dan juga digunakan untuk mengobati berbagai penyakit, terutama penyakit degeneratif. Indonesia mempunyai bahan alami yaitu berbagai minyak edible yang digunakan sebagai nutraceutical atau natural antioksidan. Penelitian ini bertujuan untuk mengkaji aktivitas antioksidan dari minyak buah merah, olive oil, vco dan minyak wijen, menggunakan metode 1,1-diphenyl-2-picrylhydrazyl (DPPH), sehingga didapatkan jenis minyak edibel yang dapat digunakan sebagai natural antioksidan. Radikal bebas  1,1-diphenyl-2-picrylhydrazyl (DPPH) digunakan sebagai salah satu metode dalam pengukuran aktivitas antioksidan. Aktivitas scavenging DPPH melalui delokalisasi elektron yang akan mengubah larutan dari kuning menjadi ungu. Metode DPPH ini merupakan metode yang sensitif untuk menguji aktivitas antioksidan Penelitian ini menggunakan empat jenis minyak edible dengan lima kali ulangan pengukuruan. Berdasarkan hasil ANOVA dan uji lanjut BNT pada taraf kepercayaan 95% maka didapatkan bahwa olive oil merupakan antioksidan yang paling kuat. Kata kunci : olive oil; vco; red fruit oil; natural antioksidan
Pigment Production of Monascus sp. Isolated from Angkak in Semarang Region, Central Java, Indonesia Endang Kusdiyantini; Soni Nugraha; Arina Lunggani
Indonesian Food and Nutrition Progress Vol 14, No 1 (2017)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ifnp.24256

Abstract

Angkak (red fungi rice, red yeast rice) is a traditional fermentation by fungi Monascus in a lot of Asia Countries, which is useful as a natural food colorant.  The aim of this research was to obtain pure isolate of red fungus from angkak in Semarang. The growth and the red pigment production of the selected isolate in the different initial medium pH and nitrogen sources were observed. The treatment was done by growing the isolate in PDB (Potato Dextrose Broth) medium at initial pH 3, 5, 7 and 9 and as well as optimization of nitrogen sources with 1% ammonium chloride, 1% ammonium nitrate, and 1% peptone. Analysis of the fungi growth was performed by weighing mycelia biomass using dry weight method and the red pigments were analysis using spectrophotometer at a wavelength (λmax) of 500 nm. The results showed that the highest absorbance value of pigment (0.81) was obtained at pH 7, and the highest value of the cell dry weight was also obtained at pH 7 (1.23 g/L). The results showed that 1% ammonium chloride was the best nitrogen source for absorbance of red pigment production (0.82), but the highest of dry weight (2.56 g/L) was obtained by using medium with 1% ammonium nitrate as a nitrogen source.
Kempong, A Traditional Fermented Food in Karangpucung Kidul village, Linggapura Bumiayu, Central Java: Fermentation Agent and Their Roles Isworo Rukmi; Devia Kusmawati Arfina; Endang Kusdiyantini
Indonesian Food and Nutrition Progress Vol 14, No 1 (2017)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ifnp.24257

Abstract

Kempong is a traditional fermented food that is found only in South Karangpucung    Linggapura Bumiayu village, Central Java. It is prepared from palm kernel cake (PKC).  This fermented food is consumed mostly everyday by the people in the village as a side dish or snack. Study on the mold important in the kempong fermentation was done by isolating molds from kempong, PKC, laru and the air of preparation room. Eleven species of molds were successfully isolated from different samples. Among the mold isolates three species came out from kempong product, i.e. R. oryzae, E. chevaliery and A. oryzae. The isolates showed the capability in producing amylase, protease, lipase, and cellulase. Both R. oryzae and A. oryzae indicated as the main fermentation mold in kempong production, because the inoculum laru only contained these two species.
Peningkatan kualitas air Waduk Diponegoro Semarang dengan filter arang aktif Nia Fadlilatul Laily; Nurhayati Nurhayati; Endang Kusdiyantini; Arina Tri Lunggani
Jurnal Penelitian Saintek Vol 27, No 1 (2022)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v1i1.48195

Abstract

Air bersih harus memiliki kualitas yang baik dari segi fisika, kimia, maupun biologisnya. Waduk Diponegoro mempunyai peran strategis dalam pengelolaan sumber daya air Kota Semarang. Berdasarkan hasil survei pada Waduk Diponegoro. Tujuan penelitian untuk meningkatkan kualitas air Waduk Diponegoro berdasarkan kategori kelas indeks kualitas air parameter biologi, fisika, dan kimia dengan perlakuan filter arang aktif. Arang aktif banyak digunakan untuk menghilangkan kontaminan dalam air karena kapasitasnya yang tinggi untuk adsorpsi senyawa tersebut, yang timbul dari luas permukaan dan porositasnya yang besar serta dapat digunakan untuk mengurangi keberadaan E. coli pada air. Proses uji bakteriologis air dilakukan dengan menggunakan metode deteksi bakteri dengan 3M Petrifilm E.coli/Coliform. Metode yang digunakan pada penelitian meliputi sterilisasi alat, pengambilan sampel air pada titik inlet dan outlet, filtrasi air dengan ketebalan arang aktif 10, 15, dan 20 cm, inokulasi sampel air, perhitungan jumlah koloni, pengukuran dan penentuan kualitas air. Hasil penelitian menunjukkan bahwa semakin tebal media arang aktif yang digunakan semakin baik kualitas air yang dihasilkan. Perlakuan filtrasi arang aktif dapat meningkatkan kualitas air Waduk Diponegoro Semarang dari kelas D kategori tercemar berat menjadi kelas C kategori tercemar sedang berdasarkan parameter biologi, fisika, dan kimia.Improve the water quality of Diponegoro Reservoir with active charcoal filterClean water must have good quality in terms of physics, chemistry, and biology. Diponegoro Reservoir has a strategic role in the management of Semarang City’s water resources. Based on the results of the survey on Diponegoro Reservoir. The purpose of the study is to improve the water quality of Diponegoro Reservoir based on the class category of water quality index of biological, physical, and chemical parameters with active charcoal filter treatment. Activated charcoal is widely used to remove contaminants in water due to its high capacity for adsorption of the compound, which arises from its large surface area and porosity and can be used to reduce the presence of E. coli in water. The water bacteriological test process is carried out using a method of detection of bacteria with 3M Petrifilm E.coli/Coliform. Methods used in the study include sterilization of tools, sampling of water at inlet and outlet points, filtration of water with the active charcoal thickness of 10, 15, and 20 cm, inoculation of water samples, calculation of the number of colonies, measurement and determination of water quality. The results showed that the thicker the active charcoal media used the better the quality of the water produced. Active charcoal filtration treatment can improve the water quality of Diponegoro Reservoir Semarang from class D category heavily polluted to class C moderately polluted category based on biological, physical, and chemical parameters.
Uji dan identifikasi aktivitas antioksidan isolat BAL CIN-2 hasil isolasi cincalok Ihdina Isfara Suteja; Wijanarka Wijanarka; Endang Kusdiyantini
Jurnal Penelitian Saintek Vol 27, No 1 (2022)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v1i1.44187

Abstract

Penelitian ini bertujuan untuk menguji antioksidan yang dihasilkan oleh isolat BAL CIN-2 dengan metode (α,α-diphenyl-β-picrylhydrazyl) (DPPH) dan fosfomolibdenum, melakukan identifikasi senyawa antioksidan yang dihasilkan isolat BAL CIN-2 menggunakan metode TLC (Thin Layer Chromatography) dan spektrofotometer UV-Vis, serta menganalisis gugus fungsional antioksidan isolat BAL CIN-2 menggunakan metode Fourier Transform Infrared Spectroscopy (FTIR). Kultur ditumbuhkan pada media MRSA dan diamati pertumbuhannya pada media MRSB selama 72 jam, selanjutnya dianalisis dengan metode yang telah ditentukan. Hasil uji TLC untuk isolat BAL CIN-2 menghasilkan empat noda dengan nilai Rf 0,98; 0,91; 0,73; dan 0,6. Uji DPPH menunjukkan sifat antioksidan supernatan isolat BAL CIN-2 sangat lemah. Metode fosfomolibdenum menunjukkan kadar antioksidan dari supernatan isolat BAL CIN-2 sebesar 1,906 mgAAE/ml. Pengujian menggunakan spektrofotometer UV-Vis menghasilkan 4 serapan gelombang dengan absorbansi maksimum 3,943 pada panjang gelombang 290 nm. Diduga senyawa yang terkandung di dalamnya adalah senyawa flavonoid golongan flavanon atau dihidroflavonol. Supernatan isolat BAL CIN-2 diduga mengandung senyawa flavonoid dengan gugus fungsi antioksidan O-H dan C=C aromatik dari analisis menggunakan FTIR. Berdasarkan hasil tersebut dapat disimpulkan bahwa di dalam supernatan isolat BAL CIN-2 mengandung senyawa antioksidan golongan flavonoid.Test and identification of antioxidant activity of isolates LAB CIN-2 isolated from cincalokThis study aimed to test the antioxidants produced by LAB CIN-2 isolates using α,α-diphenyl-β-picrylhydrazyl (DPPH) and phosphomolybdenum methods, to identify antioxidant compounds produced by LAB CIN-2 isolates using the Thin Layer Chromatography (TLC) and UV-Vis spectrophotometer, as well as to analyse the antioxidant functional groups of LAB CIN-2 isolate using the Fourier Transform Infrared Spectroscopy (FTIR) method. The culture was grown on MRSA media and then the growth was observed on MRSB media for 72 hours, then analyzed using the specified method. TLC test results showed that LAB isolate CIN-2 produced four spots with an Rf value of 0.98; 0.91; 0.73; and 0.6. The DPPH test showed that the antioxidant properties of the supernatant isolates of LAB CIN-2 were very weak. The phosphomolybdenum method showed that the antioxidant content of the supernatant of LAB CIN-2 isolate was 1.906 mgAAE/ml. Analysis using a UV-Vis spectrophotometer produces 4 absorption waves with a maximum absorbance 3,943 at a wavelength of 290 nm. The test is suspected that the compounds contained in it are flavonoid compounds of the flavanone or dihydroflavonol group. Analysis using FTIR showed that antioxidant from supernatant of LAB isolate CIN-2 was suspected to contain flavonoid compounds with aromatic O-H and C=C functional groups. It can be concluded that supernatant from LAB CIN-2 isolates contains weak antioxidants in the form of compounds from the flavonoids group.
Phylogenetic of Ornamented Chilli Based on the Fruit Morphology and Molecular Marker using OPA-12 Primer Rejeki Siti Ferniah; Endang Kusdiyantini; Nurhayati Nurhayati
Agric Vol. 30 No. 1 (2018)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2018.v30.i1.p57-62

Abstract

There are many varieties of ornamental chilli that may different from their ancestor and cause genetic variability. This research aims to determine the relationship of ornamental chillies in Indonesia based on their fruit morphology and RAPD-PCR. Morphological was analysed by description method, while molecular was analysed by RAPD-PCR using OPA 12 primer. Result showed that Katty, Naga Morich, Big Black Mama, and Yellow Primo were Capsicum chinense based on their morphology and RAPD-PCR using OPA-12 primer.
Phylogenetic of Ornamented Chilli Based on the Fruit Morphology and Molecular Marker using OPA-12 Primer Rejeki Siti Ferniah; Endang Kusdiyantini; Nurhayati Nurhayati
Agric Vol. 30 No. 1 (2018)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2018.v30.i1.p57-62

Abstract

There are many varieties of ornamental chilli that may different from their ancestor and cause genetic variability. This research aims to determine the relationship of ornamental chillies in Indonesia based on their fruit morphology and RAPD-PCR. Morphological was analysed by description method, while molecular was analysed by RAPD-PCR using OPA 12 primer. Result showed that Katty, Naga Morich, Big Black Mama, and Yellow Primo were Capsicum chinense based on their morphology and RAPD-PCR using OPA-12 primer.
Co-Authors Adde Lolita Putri Adhitya Naufal Pribadhi Afrazak Johansyah Agni Rizqy Berliyanti Agung Suprihadi Ahmad Thontowi Anggraini, Ika Anggraini, Ika Anggrayeni, Yesti Tri Anto Budiharjo Arina Lunggani Arina Tri Lunggani Arina Tri Lunggani Arina Tri Lunggani Atit Kanti Basundari, Sinta Anas Bintoro Rudi Saputro, Bintoro Rudi Budi Raharjo Daniel Pasaribu Devia Kusmawati Arfina Dian Arif Rachman E Hugeng Wandono Elawati, Nunung Eni Elawati, Nunung Eni Enny Yusuf Wachidah Yuniwarti Erma Prihastanti Euis HERMIATI Evi F Simanjuntak Faradila Ayu, Near Putri Galih Pertiwi Akbar Ginting, R Cinta Badia H Hadiyanto Hadi Endrawati Hermin P Kusumaningrum Hermin P. Kusumaningrum Hermin Pancasakti Kusumaningrum Hilmi Fadhli Ihdina Isfara Suteja Ika Anggraini Ika Anggraini Anggraini Indah Sulistyarini Joedoro Sudarsono Khabib Khasan Alfaridhi, Khabib Khasan Kurniawati, Laily Laily Kurniawati Larasati, Ella Dewani Ledy Ginting Maria Sarah Fadillah MG Isworo Rukmi Moi, Maria Yasinta Muhammad Amal Nurhakim, Muhammad Amal Muhammad Nur Muhammad Nur Muhammad Z ainuri Muhammad Zainuri Munifatul Izzati N Nurhayati Naufal, Adhitya Nia Fadlilatul Laily Novi Alvita Pratama Nugrahaini, Dian Laila Nunung Eni Elawati Nurhayati Nurhayati Nurhayati Nurhayati Nurhayati Nurhayati Prayitno, S Feta Avila Prianto, A. Heru Puteri Aryani Rejeki Siti Ferniah Rida Yuliana, Rida Saniha Adini, Saniha Sarjana Parman Soni Nugraha Sri Pujianto, Sri Sri Pujiyanto Suprihadi Suprihadi TATI NURHAYATI Tri Winarni Agustini Triwibowo Yuwono Tyas Rini Saraswati Udi Tarwotjo, Udi Wijanarka Wijanarka Wijanarka, W