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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences METAMORFOSA Journal of Biological Sciences Bioscience Jurnal Biologi Universitas Andalas Jurnal Riset Kimia Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Hilirisasi IPTEKS (JHI) Eksakta : Berkala Ilmiah Bidang MIPA Majalah Kedokteran Andalas Bioscientist : Jurnal Ilmiah Biologi
Anthoni Agustien
Jurusan Biologi, FMIPA Universitas Andalas Padang
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Pengaruh Faktor Abiotik terhadap Produksi Protease dari Isolat Bakteri M1-23 Mira Rosnawita; Anthoni Agustien; Nasril Nasir
Jurnal Biologi Universitas Andalas Vol 4, No 1 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.1.%p.2015

Abstract

A study on the influence of abiotic factors in producing protease by bacterial isolates M1-23 was conducted from May 2013 to December 2013 in the  Laboratory of Microbiology, Department of  Biology, Andalas University, Padang. This study aimed to find optimal conditions by isolate M1-23 to produce protease. This study consists of two stages. First stage used to produce the best protease and the second used to increase enzim homogenity. The result showed that the highest enzyme activity was  at medium temperature 550 C and pH 7.5. The best homogenity was produced at 125 rpm of agitation process.
Penapisan dan Karakterisasi Bakteri Amilo-Termofilik dari Sumber Air Panas Semurup, Kerinci, Jambi Wira Hastuti; Anthoni Agustien; - Nurmiati
Jurnal Biologi Universitas Andalas Vol 1, No 2 (2012)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.1.2.%p.2012

Abstract

The screening and characterization of amylo-thermophylic bacteria that produced amylase from Semurup hot spring in Kerinci, Jambi was conducted from May to July 2012. The aim of this study was to find out the isolate of amylo-thermophylic bacteria and to analyze amylase activities. This study used experimental methods. This study found 31 isolates of amylo-thermophylic bacteria with the amylolytic index more than 2. The isolate SII- 1.7 has the highest enzyme activity. The form of colony was circular margin, smooth shiny surface and pigmentation cream. The bacterium was gram negative, bacilli cell, positive catalase, non-motile, and has proteolytic and amylolytic activities.Keywords: amylolytic, bacterium, characterization, hot spring, thermophylic
Isolasi dan Karakterisasi Bakteri Alkali-Proteolitik Sumber Air Panas Semurup Kerinci Jambi Yurike Nova Edlin; Anthoni Agustien; Djong Hon Tjong
Jurnal Biologi Universitas Andalas Vol 3, No 4 (2014)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.3.4.%p.2014

Abstract

Thermophile protease has economic value because this enzyme is useful on all kind of industry that used high temperature on it production process. In the effort of obtaining indigenous alkaline thermostable a protease bacterial that produce we explore potential hot spring at Semurup, Kerinci Jambi. This study was conducted from September 2013 to February 2014, Water samples were collected by purposive sampling, the character of thermophilic bacteria were described for each in laboratory of microbiology and biomolecular, development of biology, faculty of mathematic and natural science Andalas university, Padang. There were 40 thermophile colonies, 7 isolates showed alkali proteolytic  index range from 0.3 to 2.00. SIII-10 isolate showed the highest in alkali proteolitic index. The characteristic of SIII-10 are: whute and bolt colony, gram negative, motil, positive sitrat agar and positive gelatin.Key words, Isolation, characterization, alkaline protease, thermostable, Hot spring.
Isolasi Dan Karakterisasi Bakteri Termo-Proteolitik Sumber Air Panas Sungai Medang, Sungai Penuh, Jambi Dina Wahyuna; Anthoni Agustien; - Periadnadi
Jurnal Biologi Universitas Andalas Vol 1, No 2 (2012)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.1.2.%p.2012

Abstract

A study on isolation and characterization of thermoproteolytic bacteria from hot springs at Sungai Medang, Sungai Penuh, Jambi was conducted from March to June 2012 using a descriptive method. Seventy thermophylic bacteria were isolated from the hot springs. A Total of 39 thermoproteolytic bacteria 39 were identified. The highest enzymatic activity (13.59 U/ml) was shown by isolate MI2.3 from a location at which the temperature was 600C. The isolate MI2.3 was characterized as an aerobic thermoalkaliphylic (optimum temperature of 500C, optimum pH of 9.0) bacterium with the ability to produce amylase but neither lipase nor cellulase.Keywords : alkaliphylic, bacterium, enzymatic activity, proteolytic, thermophylic.
Isolasi dan Karakterisasi Bakteri Amilotermofilik Dari Sumber Air Panas Sungai Medang Rahmadani Eka Pitri; Anthoni Agustien; Fuji Astuti Febria
Jurnal Biologi Universitas Andalas Vol 4, No 2 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.2.%p.2015

Abstract

The research on isolation and characterization of amylothermophilic bacteria has been done in Research Laboratory of Microbiology, Biology Department, Mathematics and Natural Sciences Faculty, Andalas University, Padang. The aimed to obtain amylothermophilic isolate and describe their characteristic. The study used purposive sampling method and descriptive analysis. The study results obtained four amylothermophilic isolates with amylolitic index ranged from 2.23 until 3.51 were different macroscopic characters, rod shape, motile and produce catalase enzyme, three isolates  were  Gram positive and spore forming, one isolate was  Gram negative.
Antibacterial potency of fresh extract leaves of jamaican cherry (Muntingia calabura L.) in Inhibiting the growth of Shigella dysenteriae. Ikel Fitri Yanis; Feskaharny Alamsjah; Anthoni Agustien; Tesri Maideliza
Jurnal Biologi Universitas Andalas Vol 8, No 1 (2020)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.8.1.14-19.2020

Abstract

Research on the antibacterial potency of fresh extract from leaves of Jamaican cherry (Muntingia calabura L.) in inhibiting the growth of Shigella dysenteriae had been conducted in the Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University. The study aimed to determine the potential of fresh extract from  Jamaican cherry leaves in inhibiting the growth of S. dysenteriae and to determine its Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) against S. dysenteriae. The results showed that the fresh extract of Jamaican cherry leaves was potent as an antimicrobial agent against pathogenic bacteria S. dysenteriae. It was shown through 12 - 14 mm diameter of inhibition zone which was classified as strong inhibition. The MIC was measured at 3.125% while MBC was undetermined. According to these findings, it can be concluded that the fresh extract from Jamaican cherry leaves was potent to inhibit the growth of S.dysentriae at 3.125% concentration, yet unable to kill it.
Penapisan dan Karakterisasi Bakteri Selulolitik Termofilik Sumber Air Panas Sungai Medang, Kerinci, Jambi Uci Mela Sari; Anthoni Agustien; - Nurmiati
Jurnal Biologi Universitas Andalas Vol 1, No 2 (2012)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.1.2.%p.2012

Abstract

A study on screening and characterization odf cellulolytic thermophylic bacteria from Sungai Medang hot spring was conducted on May to June 2012 using purposive sampling methods. This study aimed to find a cellulolytic thermophylic bacterium and to analyze its cellulolyties. Twenty eight cellulolytic thermophylic bacteria were isolated from Sungai Medang hot springs. The highest enzymatic activity (amylolytic and proteolytic activity) was shown by isolate MII2.1 from a location at which the temperature was 78oC. This isolate was characterized as an aerobic bacterium, gram negative and non motile. The form of colony was a circular margin, smooth shiny surface and elevation flat.Keywords: amylolytic, bacterium, cellulolytic, proteolytic, thermophylic
Penapisan Bakteri Termo-Amilolitik Dari Sumber Air Panas Sungai Medang, Kerinci, Jambi Devi Syafriyani; Anthoni Agustien; - Periadnadi
Jurnal Biologi Universitas Andalas Vol 2, No 2 (2013)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.2.2.%p.2013

Abstract

This study aimed to observe the ability of isolated bacteria from Sungai Medang hot spring in producing amylase and to determine the activity and characteristic of isolated amylolytic bacteria which gave the highest amylolytic index. The water samples were collectied by using purposive sampling based on different level of temperatures. This study has successfully collected 48 bacterial isolated from the hot spring. To find the highest amylolytic activity, we tested ten isolated bacteria which have high amylolytic indices. We found that MV2.7 isolated from 740C of hot spring gave the highest amylolytic activity. The isolated bacteria was positive proteolytic, cellulolytic and negative lipolytic. The isolated bacteria showed higher and more spesific activity on rice starch rather than corn and sago starchKeywords: Screening, thermophilic, amylolytic, activity
PRODUKSI PROTEASE ALKALI DAN KERATINASE DARI Brevibacillus agri A-03 TERMOFILIK Anthoni Agustien; Jetty Nurhajati; Linar Z. Udin; Pingkan Aditiawati
Jurnal Riset Kimia Vol. 4 No. 1 (2010): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v4i1.44

Abstract

 ABSTRAK Protease alkaline and keratinase are a group of protease enzym which have important value in detergen industry and skin tannery. Brevibacillus agri A-03 is thermophilic bacteria isolate that comes from Ambayan Sumatera Barat hot spring and has the ability to produce protease and keratinase. The purpose of this research is to get protease alkaline and thermostable keratinase from Brevibacilus agri-A03. thermostable enzym is produced from enzym production enzym that contains kasein and keratin at various medium pH, inoculum incubation temperature and medium type. Enzym activity is measured by modified Walker methode, protein content is measured by Lowry methode. Protease alkaline is produced at exponential phase, maximum at 18th hours of incubation and keratinase is produced at stationer phase, maximum at 22nd hours. Both enzym is produced optimically at medium pH condition 9.0; incubation temperature 55°C, inoculum 5% by using modified Johnvesly and Naik medium with each protease and keratinase specific activity 1.927 and 1.047 U/mg  Keywords: Protease alkaline, Keratinase, Thermofilic, Brevibacillus agri A-03   
Potensi Jamur Endofit pada Tumbuhan Mangrove Avicennia marina di Kuala Enok Indragiri Hilir sebagai Penghasil Antibiotika Kustiasih Lestari; Anthoni Agustien; Akmal Djamaan
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p13

Abstract

This study provides an overview of the potential of endophytic fungi isolated from leaves, stems, mangrove roots A. marina as a producer of antibiotics tested for Staphylococcus aureus, Escherichia coli, Candida albicans. This research was carried out in the microbiology laboratory of the Health Polytechnic of the Ministry of Health of Riau from March to June 2017. This study used a laboratory experimental method, the data presented in descriptive form. The stages of the study were: identification of A. marina plants, fungal isolation, qualitative tests of flavonoids isolates from endophytic fungal, and antibacterial activity test. The results showed that from 15 isolates of endophytic fungi, 5 isolates producing antibiotics. FAA 3 isolate showed the highest antibiotic activity with 24 mm the diameter of the inhibitory zone, while the lowest inhibition zone was FAA 4 isolate by forming a diameter of inhibitory zone of 7 mm and the results of qualitative tests of metabolites showed as secondary flvonoid metabolites. This research can be used as a source of information for the community in utilizing mangrove plants as an alternative in improving health.
Co-Authors - Nurmiati - Nurmiati - Nurmiati Aghni Aznia Akmal Djamaan Armaleni Armaleni Ayu Putri Ningsih Chairul Chairul Cici Wulandari Desma Yuni Deswati Deswati Devi Syafriyani Dianty Putri Dina Wahyuna Djong Hon Tjong Efrizal Efrizal Efrizal Efrizal Fadhila Cantika Ferry Lismanto Syaiful Feskaharny Alamsjah Fuji Astuti Febria Ikel Fitri Yanis Ilsa Septia Putri Inayati Rahmi Irdawati Irdawati Irfan Mustafa irfan mustafa Jetty Nurhajati Kustiasih Lestari Linar Z. Udin Lola Alviche Melisa Ainil Fajri Mira Rosnawita Nasril Nasir Nasril Nasir Nasril Nasir Nasril Nasir Nasril Nasir Nurhajati, Jetty Nurmiati Nurmiati Nurmiati Nurmiati Periadnadi Periadnadi Pingkan Aditiawati PINGKAN ADITIAWATI Rahmadani Eka Pitri Rahmi Adila Riski Budi Yani Rusnam Rusnam Suwirmen, Suwirmen Syamsuardi Syamsuardi Syukria Ikhsan Zam Syukria Ikhsan Zam Tesri Maideliza Tri Widia Ningsih Alam Uci Mela Sari Udin, Linar Z. Widia Firliani Wira Hastuti Yetria Rilda Yurike Nova Edlin Zakaria, Indra Junadi Zuhri Syam Zuhri Syam