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All Journal Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Kedokteran Hewan

i Nengah Wandia

Laboratorium Anatomi Bagian Anatomi Fakultas Kedokteran Hewan Universitas Udayana, Denpasar

Author-ID : 284335

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Identifikasi Spesies Ikan Kerapu di Pasar Ikan Karangasem dan Kedonganan Bali Menggunakan DNA Mitokondria Gen 16s rRNA IDENTIFICATION OF GROUPER SPECIES USING 16S rRNA MITOCHONDRIAL DNA IN KARANGASEM AND KEDONGANAN FISH MARKET, BALI Ni Luh Made Ika Yulita Sari Hadiprata; I Made Bagus Arya Permana Ardiana Putra; I Gusti Ngurah Kade Mahardika; I Nengah Wandia; Tjokorda Sari Nindhia
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Groupers are bio-indicators of the health of coral ecosystems and high economic value. Various speciesof grouper fish sold in markets in Indonesia is not known. The purpose of this study was to identify thespecies of grouper fish market located in Karangasem and Kedonganan. The method used in this studywas polymerase chain reaction(PCR) techniqueand mitochondrial DNA 16S rRNA gene as a molecularmarker. Grouper samples were collected from the fish market Karangasem total of 11 samples and 42samples collected from Kedonganan. Sequences from each sample was obtained from the analysis inBerkeley Sequencing Facility, California. Sequencing samples were then analyzed by BLAST and matchedwith the sequences found in GenBank. The analysis showed that six species can be identified from the fishmarket Karangasem namely Cephalopholis leopardus, C.cyanostigma, C. miniata, C. sonnerati, Variolaalbimarginata, and Epinephelus fasciatus. Seven species can be identified in the fish market Kedonganannamely E. fuscoguttatus, E. coeruleopunctatus, E. merra, E. polyphekadion, C. cyanostigma, C. miniata andV.albimarginata.

Karakteristik Molekuler Virus Avian Orthoavulavirus 1 Genotipe VII yang Diisolasi dari Tabanan Bali (MOLECULAR CHARACTERISTIC OF AVIAN ORTHOAVULAVIRUS 1 GENOTYPE VII ISOLATED FROM TABANAN BALI) Anak Agung Ayu Mirah Adi; I Nyoman Mantik Astawa; I Nengah Wandia; I Gusti Agung Arta Putra; Ida Bagus Oka Winaya; Anak Agung Keswari Krisnandika; Anak Agung Oka Wijaya
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Newcastle disease (ND) is a very harmful avian disease, endemic in Indonesia and various parts of the world. The causative agent is ND virus or Avian orthoavulavirus 1 (AOAV-1). This virus is an RNA virus with wide genetic variation. Based on the genome length, it can be classified into AOAV-1 Class I and II. Class I are generally avirulent whereas Class II are consist of both virulent and avirulent viruses, currently there are 18 genotypes of the class II. To find out the molecular characteristics of AOAV-1 currently circulating in the field, isolation and identification of viruses from laying hens that was suspected ND from Tabanan Bali in 2017, was performed. The isolated viruses hereafter named as Tabanan1/ARP / 2017. A one-step RT-PCR reaction was carried out to amplify NP, F and HN gene fragments from the virus using three specific pairs of AOAV-1 primers. The obtained nucleotide sequences are then used in phylogenetic analysis. For phylogenetic analysis several strains of AOAV-1 from class II representing genotype I-VII as well as one strain from Class I were accessed from GenBank. From the analysis of the F gene nucleotide sequences, it was found that Tabanan 1 / ARP / 2017 is a genotype VII virus with an amino acid sequence at the F protein cleavage site is 112 R-R-Q-K-R-F117, a typical virulent strain. Phylogenetic analysis using nucleotide sequences NP and HN genes also positioned this isolate in genotype VII. At the nucleotide level, genetic distance with virulent isolates that was isolated in 2007 and 2010 were 8.26% and 1.08% while at the amino acid level were 5.26% and 0.64%. There were found mutations in amino acids at positions 107 and 108 of F protein.

Variasi Genetik Populasi Monyet Ekor Panjang (Macaca fascicularis) di Pulau Nusa Penida, Klungkung, Bali (GENETIC VARIATION OF LONG TAIL MACAQUE (MACACA FASCICULARIS) POPULATION IN NUSA PENIDA ISLAND, KLUNGKUNG, BALI) Elisabeth Yulia Nugraha; I Nengah Wandia; I Gede Soma
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The purpose of this study is to discover about heterozygosity, migration between populations, and mating habits of Macaca fascicularis among the population in Nusa Penida Island, Klungkung, Bali. Fourteen blood samples from Macaca fascicularis were taken from Nusa Penida Island that consist of eleven samples from Puncak Mundi Temple and three samples from Paluang Temple were used in the study. Samples were extracted using QIAamp DNA Blood Mini Kits produced by Qiagen. Five loci microsatellite (D10S611, D11S1366, D13S765, D17S1290, and D18S536) were amplified using the Polymerase Chain Reaction (PCR) technique. Thirty cycles of PCR were carried out with an annealing temperature of 500C. Alleles were separated by electrophoresis on eight percent polyacrylamide gel and presented with silver staining. The results showed high heterozygosity is 0.762 of Macaca fascicularis populations on Nusa Penida Island with random mating habits. Low genetic differentiation is 0.05. High the number of migrations between populations (genetic flow) of Macaca fascicularis populations on Nusa Penida Island is 4.75 with migration between populations of five individual/generation. Based on the results of the study it was concluded that the genetics of the Macaca fascicularis population on Nusa Penida Island varies.

Urine Chemistry Profile of Captive Sumatran Elephant (Elephas maximus sumatranus) in Bali Elephant Camp, Carangsari, Petang, Badung, Bali Theresa Utami; I Nengah Wandia; Nyoman Sadra Dharmawan
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Sumatran elephant (Elephas maximus sumatranus) is the biggest land mammals in Indonesia in criteria A2c category Critically Endangered (CR) dan Appendix I in Convention on International Trade in Endangered Species of Wild Flora and Fauna (CITES). Considering the importance of conservation and health maintenance of Sumatran elephants, various diagnostic tests must be carried out to determine the health status of elephants ranging from physical examination to blood chemistry test and urinalysis.The aim of this study was to obtain the chemical profile of the Sumatran elephant (Elephas maximus sumatranus) which is captive in the Bali Elephant Camp, Carangsari, Petang, Badung Regency, Bali. The urine sample taken is mid stream (middle emission) urine. Sampling was carried out 5 times for 2 weeks with 2-3 days distance between samples taken and the dipstick test was carried out twice in one sample. Based on the results of this study, it can be concluded that of the 8 Sumatran elephants maintained at the Bali Elephant Camp all are 100% negative for glucose. Meanwhile, protein levels were found in 5 individuals (62.5%) in the range 0-2000. Positive bilirubin and hematuria were found in one individual (12.5%). Ketone levels are found in the range 0-160 in 7 individuals. A total of 84.7% of urobilinogen data (mg / dL) is 0.2. The pH value is in the range of 6-9, while the value of density is in the range 1-1,020. Leukocyte level (Leu / µL) is worth 70 (91.3%).

Pembiusan Monyet Ekor Panjang (Macaca fascicularis) dengan Campuran Ketamine dan Xylazine (THE ANAESTHETIZATION OF LONG TAILED MACAQUE (MACACA FASCICULARIS) BY INJECTING THE COMBINATION OF KETAMINE AND XYLAZINE) I Nyoman Suartha; I Nengah Wandia; I Gusti Agung Arta Putra; I Gede Soma; I Gusti Ngurah Sudisma
Jurnal Veteriner Vol 2 No 1 (2001)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Keragaman Spesies Ikan Tuna di Pasar Ikan Kedonganan Bali dengan Analisis Sekuen Kontrol Daerah Mitokondria DNA (SPECIES DIVERSITY OF TUNA FISH USING MITOCHONDRIAL DNA CONTROL REGION SEQUENCE ANALYSIS AT KEDONGANAN FISH MARKET) Daud Steven Triyomi Hariyanto; I Gusti Ngurah Kade Mahardika; I Nengah Wandia
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Tuna is an export commodity which has very high economic value. However, some tuna speciesare threatened with extinction. The purpose of this study was to identify the tuna species that aresold in Kedonganan Fish Market. The research method was polymerase chain reaction technique(PCR) using the marker sequence mitochondrial DNA control region. Samples were obtained fromthe Fish Market tuna Kedonganan, Kuta, Badung, Bali. The total number of samples are 28specimens. Sequence from each sample was obtained through sequencing techniques. Sequencesobtained were run in BLAST (Basic Local Alignment Search Tool) and subsequently analyzed withMEGA 5 for species confirmation. Three species of tuna that are identified in the Kedonganan FishMarket is: Thunnus albacares, T. obesus, and Katsuwonus pelamis. All three species have highgenetic variation HD = 1. This study needed to be continued with more number of samples todetermine the species of tuna sold in Kedonganan Fish Market.

Pembiusan Monyet Ekor Panjang (Macaca fascicularis) Jantan dengan Campuran Ketamin dan Xilazin pada Topografi Daerah Berbeda (THE ANAESTHETIZATION OF LONG TAILED MACAQUE (MACACA FASCICULARIS) BY KETAMINE-XYLAZINE ON DIFFERENT TOPOGRAPHIC AREA) I Nyoman Suartha; I Gusti Agung Arta Putra; I Nengah Wandia
Jurnal Veteriner Vol 4 No 1 (2003)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Co-Authors Abd. Rasyid Syamsuri Agus Wawan Darmawan Aida Lousie Tenden Rompis Alda dasril lumban gaol An'nisafitri Lutviana Anak Agung Ayu Mirah Adi Anak Agung Gde Jaya Wardhita, Anak Agung Gde Jaya Anak Agung Keswari Krisnandika Anak Agung Oka Wijaya Anak Agung Wisnu Kusuma Putra Andini, Ni Made Ria Ayu Ratnasari BASYOFI DWIWANDANA Bellantari, Melinda Darmawan, I Putu Gede Buda Daud Steven Triyomi Hariyanto Debora Selfia Br Manurung DEWA AYU PUTU ARIE SERATHAN SUPARTHA Dini Maharani Evi kumala dewi Febio Tomasini Marciano Meus Fedri Rell Gde Angga Caka Primanditha, Gde Angga Caka Gurning, Santri Devita Sari Hasanah, Putri Nur Hidayah, Dinda Nur I GA Artha Putra I Gede Soma I Gusti Agung Arta Putra I Gusti Agung Ayu Suartini I Gusti Agung Gede Putra Pemayun I Gusti Ngurah Kade Mahardika I Gusti Ngurah Sudisma I K. SUATHA I Ketut Anom Dada I Ketut Puja I Ketut Suatha I Made Bagus Arya Permana Ardiana Putra I Made Dwinata I NYOMAN ADI SURATMA I NYOMAN MANTIK ASTAWA I Nyoman Suartha I Nyoman Sulabda I Putu Gede Yudhi Arjentinia I Wayan Batan I Wayan Wirata I. B. WINDIA ADYANA Ida Bagus Oka Winaya Ida Bagus Windia Adnyana Jefri Ndawa Rumba Made Rahayu Kusumadewi Made Raysa Merliana Maharani, Nisa Mahmud Siswanto Mahmud Siswanto MIKEU PAUJIAH MUR MUAZIN MUAZIN Muhadjir, Iin Mutmainnah Murdayasa, I Wayan Gde Ni Luh Made Ika Yulita Sari Hadiprata Ni Nyoman Trinayani Ni Nyoman Trinayani Ni Nyoman Trinayani Ni Wayan Patmawati Ni Wayan Patmawati Ni Wayan Patmawati Nugraha, Elisabeth Yulia Putu Suastika Rahmiati, Nur Ilmi Risha Catra Pradhany Rostiani Silta RUSMI AKIRA S. K. WIDYASTUT Saputra, Muhammad Rama Imam Saulina, Renata Sendana, Lois Sihombing, Tri Indra Erikson Silaban, Root Elisa Sri Kayati Widyastuti Suastika , Putu Theresa Utami Tjokorda Sari Nindhia WAODE SANTA MONICA Wibisono, Hanif Wahyu Wulandari Wulandari Yesy Febnica Dewi Yulianty, Syifaurrachmah ZAKIATUN MUHAMMAD

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