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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Jurnal Primatologi Indonesia Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Hemera Zoa Media Veteriner Forum Pasca Sarjana Jurnal Sain Veteriner Buletin Veteriner Udayana Jurnal Veteriner Biologi Edukasi : Jurnal Ilmiah Pendidikan Biologi Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Ilmu Ternak dan Veteriner Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) ARSHI Veterinary Letters Jurnal Kedokteran Hewan
Srihadi Agungpriyono
Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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Journal : HAYATI Journal of Biosciences

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Sperm Preservation using Freeze-Drying Method TAKDIR SAILI; MULYOTO PANGESTU; MOHAMAD AGUS SETIADI; SRIHADI AGUNGPRIYONO; MOZES R. TOELIHERE; ARIEF BOEDIONO
HAYATI Journal of Biosciences Vol. 12 No. 1 (2005): March 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (32.025 KB) | DOI: 10.4308/hjb.12.1.41

Abstract

Since the discovery of cryopreservation method for bull semen, cryopreservation become an alternative method for maintaining gamet resources of certain animal which is threatened or near extinction. This technology was then applied to the preservation of embryo, oocyte, ovary and testis. The application of intracytoplasmic sperm injection (ICSI) for which sperm motility is unnecessary had supported the effort to create simplified method such as freeze-drying for sperm preservation. Due to the benefit of ICSI over the conventional in vitro fertilization (IVF) the spermatozoon could be mechanically driven to pass through the zona pellucida and entering the cytoplasm of oocytes prior to fertilization. The freeze-drying method is an alternative method in sperm preservation which ignored the motility of sperm. The sperm resulted from this technique is in drying state, therefore, it might be stored in room temperature or in refrigerator. Many reports have claimed that freeze-dried sperm which is not motile but has an intact DNA was able to fertilize oocytes, even produced offspring in mouse.
In Vitro Fertilization and Embryo Development ITA DJUWITA; ARIEF BOEDIONO; SRIHADI AGUNGPRIYONO; IMAN SUPRIATNA
HAYATI Journal of Biosciences Vol. 12 No. 2 (2005): June 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (86.772 KB) | DOI: 10.4308/hjb.12.2.73

Abstract

Experiments were conducted on the morphology, fertilization and embryo development rate of vitrified ovine oocytes matured in vitro. Three vitrification solutions were used for vitrification. PBS supplemented with 1% BSA, 30% ethylene glycol was added by one of three different sucrose concentrations, 1.00 M (VS1), 0.50 M (VS2), and 0.25 M (VS3). The results showed that the percentages of normal vitrified oocytes after warming were 78 and 63% in VS1 and VS2, respectively, which was significantly higher as compared for VS3. The fertilization rates were 59 and 66% in VS1 and VS2, respectively, which were also significantly higher as compared with VS3 (35%). Zygote viability after 18 h was 57; 43; and 40%, for VS1,VS2, and VS3, respectively, which was not significantly different. The incidence of polyspermic penetration increased with increasing sucrose concentration, i.e 23, 11, and 9% in VS1, VS2, and VS3, respectively, as compared with unvitrified oocytes (4%). The cleavage rate of vitrified oocytes in VS1 was 13.2% which was significantly lower (p
Co-Authors A.S. Satyaningtijas Adi Winarto Adrien Jems Akiles Unitly Alastair A Macdonald Amrozi - Anak Agung Istri Sri Wiadnyani Andhika Yudha Prawira Andhika Yudha Prawira Andhika Yudha Prawira Andhika Yudha Prawira Andhika Yudha Prawira Angelina N. Tethool Anisa Rahma Arief Boediono Aries Boediono Aryani Sismin Sastyaningtijas Asri Pudjirahaju Auzi Asfarian Chairun Nisa Chairun Nisa Chairun Nisa' Chairun Nisa’ Cutnya’ Shaliran Nazlie (Alm) Damiana Rita Ekastuti Danang Dwi Cahyadi Danang Dwi Cahyadi Danang Dwi Cahyadi DENI NOVIANA Denny W. Lukman Desrayni Hanadhita Desrayni Hanadhita Desrayni Hanadhita Desrayni Hanadhita Dondin Sajuthi Dwi Endrawati Dwi Kesuma Sari Dwinna Aliza Erdiansyah Rahmi Erni Sulistiawati Etty Riani Evalina E Fadli A. Gani Gholib Gholib Gunanti . Hafizuddin Hafizuddin Halim Bakti Harjo Hamny Sofyan Helny Rosita Supriadi Helny Rosita Supriadi HERA MAHESHWARI Heru Setijanto Heru Setijanto Huda Salahudin Darusman Huda Salahudin Darusman I Ketut Mudite Adnyana ICHSAN EFFENDIE Idawati Nasution Idawati Nasution Iman Supriatna Ita Djuwita Ita Djuwita Junzo Yamada Ketut Adnyane Mudite Kusdiantoro Mohamad Lidya Elizabeth M. Manik M Agus Setiadi M. Haviz M. Zairin Junior Masaji Washio Md Zuki Abu Bakar Mirnawati Sudarwanto Mokhamad Fahrudin Mokhamad Fakhrul Ulum MOZES R. TOELIHERE MOZES TOELIHERE MUHAMMAD AGIL Muhammad Jalaluddin Muhammad Risman Wahid Mulyadi Adam MULYOTO PANGESTU Muslim Akmal Nabuo Kitamura Najamudin - Nastiti Kusumorini Nastiti Kusumorini Nazlie (Alm), Cutnya’ Shaliran Ni Wayan Kurniani Karja Noordin Mohamed Mustapha Nurhidayat Odilia Rovara R. Iis Arifiantini Razali . RIDWAN AFFANDI Riki Siswandi Ronny Rachman Noor Rusdin - Savitri Novelina Sri Wahyuni Sri Wahyuni Sriyanto - Supratikno Supratikno . Supratikno Supratikno Syahruddin Said TAKDIR SAILI Takdir Syahruddin Said Tongku Nizwan Siregar TUTI L YUSUF Tutik Wresdiyati - Tuty L. Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Ursula Paulawati Maker Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahyuni, Sri Wartika Rosa Farida Wartika Rosa Farida Yoshio Yamamoto Yuliani Suparmin