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Efek Antifungi Seduhan Teh Hijau (Camellia sinensis L.) terhadap Pertumbuhan Candida albicans In Vitro Wardhani, Irene Ardiani Pramudya; Haryati, Sri; Setyawan, Novan Adi
Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika
Publisher : Nexus Biomedika

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Background : Brewed green tea contains antifungal substance such as polyphenol which is considered can inhibit fungal growth.Â The aim of this research is to determine antifungal effect of brewed green tea (Camellia sinensis L.) against Candida albicans growth In Vitro. Methods : The research was performed as experimental laboratory. The subject of this research were clinical sample of Candida albicans and taken randomly. This research used 7 treatment groups, they were sterilized aquadest as negative control, brewed green tea with concentration of 60%, 70%, 80%, 90% and 100%, also fluconazole 25 Âµg as positive control. This research was repeated four times. The plate was incubated at the temperature of 37ÂºC for 48 hours then inhibition diameter zone formed was measured. The data was analyzed by Linier Regression test then continued by Correlation test on IBM Statistics for Windows version 20. Results : The diameter of inhibition zone increased along with increasing concentrations, because higher concentration of brewed green tea contained more substances, and the diameter of concentration of 100% group is almost the same with diameter of positive control group. The results of statistical analysis using Linier Regression test showed that there are significant differences on seven of treatment groups (p < 0,05)Â and the Correlation test showed that there are close relation between concentration of brewed green tea and the diameter of inhibition zone. Conclusion : Brewed green tea (Camellia sinensis L.) has antifungal effect against Candida albicans growth In Vitro. Start from concentration of 60% up to 100%, there were antifungal effect, and the antifungal effect of concentration of 100% is close to Â fluconazoleÂ 25 Âµg/ml. Keywords : Antifungal Effect, Brewed Green Tea, Candida albicansÂ

Hubungan Diabetes mellitus dengan Hasil Uji Konversi BTA Sputum setelah Pemberian Terapi Tuberkulosis Tahap Inisiasi Pranoto, Avamira Rosita; Sutanto, Yusup Subagio; Haryati, Sri
Nexus Kedokteran Klinik Vol 3, No 1 (2014): Nexus Kedokteran Klinik
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Pulmonary tuberculosis (TB) is a chronic infection of lung caused by Mycobacterium tuberculosis. Tuberculosis treatment often gets failure in result because of lack of care in co-morbid disease such as Diabetes mellitus (DM), which can disturb TB patient immunity. The objective of this study was to determine the relation of Diabetes mellitus and result of conversion test of sputum with alcohol-acid resistant bacillus after initiation phase of tuberculosis medication. Methods: This was an observational analytic survey with retrospective cohort study. Using consecutive sampling method, this study took 124 patients from RSUD Dr. Moewardi and BBKPM (Balai Besar Kesehatan Paru Masyarakat) Surakarta, who divided into 2 groups based on the DM status of newly diagnosed pulmonary TB patients. Control group consisted of 86 newly diagnosed pulmonary TB patients without DM; exposure group consisted of 38 newly diagnosed pulmonary TB patients with DM. Patients from both groups were given category-1 tuberculosis treatment during 2 months of initiation phase therapy, then the BTA conversion status of patients was observed. Data collected through the medical record and concurrent data of pulmonary TB patients. Data was analyzed by SPSS using Chi Square test. Probability (p) is < 0.05 with 95% confident interval. Results: There were 14 patients from the control group who failed to undergo conversion after 2 months of initiation phase therapy, whereas from the exposure group, there were 17 patients who got failure in result. Data analysis using Chi Square with significance level p < 0.05 was obtained p = 0.001 and Relative Risk (RR) = 0.66. Conclusions: DM seems to have a negative impact on the outcome of tuberculosis treatment. Pulmonary tuberculosis patients with DM as comorbid disease has probability 0.66 times lower than those without DM to get positive result from BTA sputum conversion test. Screening for DM and subsequent blood glucose control may improve the outcome of tuberculosis treatment. Keywords: Diabetes mellitus, tuberculosis medication, result of conversion test of sputumÂ

Pengaruh Ekstrak Daun Salam (Syzygium polyanthum Wight) Terhadap Waktu Kematian Ascaris suum,Goeze In Vitro Andaru P, Gagat Ragil; Sakiman, Bambang Sukilarso; Haryati, Sri
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Syzygium polyanthum Wight leaves contain tannin that has been known to have anthelmintic effect. This study aimed to determine the effect of Syzygium polyanthum Wight leaves extract toward death time of Ascaris suum, Goeze In Vitro Methods: The study was a laboratory experimental research using the post-test only controlled group design. Subjects were adult Ascaris suum, Goeze. The sampling technique used was purposive sampling. Subjects were divided into 7 groups, each group consisted of 4 worms, replication was performed 4 times. Saline solution was used in negative control group, pirantel pamoate 5 mg/ml was used in positive control group, while the treatment group used Syzygium polyanthum Wight leaves extract concentration of 70%, 75%, 80%, 85%, and 90%. Worms were immersed in 25 mL of the test solution and incubated at 37Â°C. Observations were done every 1 hour and the number of dead worms was counted. Data were analyzed with regression linier and probit analysis. Results: Observations of total deaths mean time Ascaris suum, Goeze sp on negative control was for 96 hours, on the positive control was for 2 hours 30 minutes, and on the concentration of 70%, 75%, 80%, 85%, and 90% of Syzygium polyanthum Wight leaves extract, each was for 10 hours 15 minutes, 8 hours, 6 hours, 4 hours 45 minutes and 3 hours, consecutively. Linear regression test results showed that the variation of the concentration affected long worm death. Probit analysis results showed that LC50 and LT50 of Syzygium polyanthum Wight leaves extract, each was 76.228% and 3 hours 36 minutes. Conclusions:Based on this study, it could be concluded that of Syzygium polyanthum Wight leaves extract affected mortality of Ascaris suum, Goeze sp in vitro. The increasing of extract concentration was inversely proportional with the death time of worms Keywords: Syzygium polyanthum Wight leaves extract, Ascaris suum,Goeze.

Efek Antihelmintik Ekstrak Akar Ceguk (Quisqualis indica, L.) pada Ascaris suum, Goeze in Vitro Kurnia, Antonius Bagus Budi; Haryati, Sri; Sakiman, Bambang Sukilarso
Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Radix-Quisqualis indica L. contains saponins and potassium quisqualata that are known having anthelmintic effect. This study aimedÂ to determine the effect of extract of radix-Quisqualis indica L. toward mortality of Ascaris suum, GoezeÂ in vitro Methods: This study was a laboratory experimental research using the post-test only controlled group design. Subjects were adult Ascaris suum, Goeze. The sampling technique used was purposive sampling. Subjects were arranged into 7 groups, each group consisted of 4 worms, replications were performed 4 times. Saline solution was used in negative control group and pyrantel pamoate 5 mg/ml was used in positive control group, while the treatment group was treated with extract of radix-Quisqualis indica, L. at concentration of 50%, 60%, 70%, 80%, and 90%. Subjects Â were immersed in 25 ml of Â test solution and incubated at 37Â°C. Observations were done every 1 hour and the numbers of dead worms were counted. Data were analyzed with linier regression and probit analysis. Results: The result of observation showed that mean time of total death of Ascaris suum, GoezeÂ in the negative control and in the positive control were 96.00 hours and 1.25 hours, each. While mean time of total death of Ascaris suum, Goeze in the extract of radix-Quisqualis indica, L. at concentration of 50%, 60%, 70%, 80%, 90% were 11.50 hours, 9.50 hours, 8.00 hours, 5.75 hours and 3.50 hours, consecutively. Shapiro Wilk test showed that the distribution of the data was normal (p > 0.05). Linear regression test showed that the variation of the extract concentration affected time of worm death. Probit analysis showed that LC50 and LT50 of the extract of radix-Quisqualis indica L. were 69.80% and 3.90 hours. Conclusions: The extract of radix-Quisqualis indica, L. affected mortality of Ascaris suum, GoezeÂ in vitro. Keywords: extract of radix Quisqualis indica, L., Ascaris suum, Goeze,

Pengaruh Ekstrak Etanol Daun Alpukat (Persea americana Mill.) terhadap Mortalitas Cacing Ascaris suum, Goeze in Vitro Astarani, Monica Citraningtyas; Sukilarso, Bambang; Haryati, Sri
Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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BackgroundÂ : Avocado leaves which have been known from phytochemical contain flavonoid, tannin katekat, quinone, saponin, and steroid/triterpenoid. Saponin and tannin are active substances which have anthelmintic effect. This research was to know the effect of avocado (Persea americana, Mill.) leaves ethanol extract toward the mortality of Ascaris suum, Goeze in vitro. Methods : This study was experimental laboratory research with the post test only controlled group design. The subjects were 150 adult Ascaris suum, Goeze, arranged into 6 groups. NaCl 0.9 % solution was treated to negative control group, while 5 mg/ml pirantel pamoat solution was treated to positive control. The others group were treated with 60%, 70%, 80%, 90% w/v of Avocado (Persea Americana, Mill.) leaves ethanol extract. Observation was done in every hour until all worm died. The data were analyzed by linear regression and probit analysis. ResultsÂ Â : Probit analysis showed that LC50 and LT50 of Avocado leaves extract were 56.42% dan 665.15 minutes. Linear regression analysis showed there was Â significant (p < 0.05) effect between the time of death and variation of extract concentration. Conclusion : Avocado (Persea americana Mill.) leaves ethanol extract had significant effect toward the mortality of Ascaris suum, Goeze in vitro. Keywords : avocado leaves ethanol extract, Ascaris suum, LC50

Perbedaan Daya Anthelmintik antara Ekstrak Daun Pepaya (Carica papaya, Linn.), Daun Pare (Momordica charantia, Linn.), dan Kombinasi Keduanya terhadap Cacing Ascaris suum, Goeze in Vitro Swadini, Nurul Rahmawati; Haryati, Sri; Sakiman, Bambang Sukilarso
Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Backgrounds: Ascariasis is an infection disease caused by Ascaris lumbricoides, Linn. Drugs therapy for ascariasis has various side effects causing unpleasant sensation to the patient such as nausea, vomiting, diarrhea, and constipation. Infusion of papaya leaves and bitter gourd leaves contains saponin and tannin known having anthelmintic activity. This experiment aimed to find out the difference of anthelmintic capacity among papaya leaves extract, bitter gourd leaves extract and their combination to Ascaris suum, Goeze, in vitro. Methods: This experiment was quasi experiment with post test only controlled group design. Subject for this experiment was Ascaris suum, Goeze. The subject was arranged into five groups, those were negative control group (NaCl 0.9%), positive control group (Pirantel pamoat 5 mg/ml), papaya leaves extract group, bitter leaves extract group and the combination of both papaya and bitter gourd leaves extract group. The extract concentrations for the treatment were 40%, 50%, 60%, 70%, and 80% w/v. Subjects were dipped into extract for maximum monitoring time. Death time of all worms in each group was examined. Data were analyzed with linear regression and probit analysis (Î± = 0.05). Results: Regression analysis showed that there was a significant relation between death time and concentration of papaya leaves extract, bitter gourd leaves extract, and combination of both with significance value p = 0,000 (p < 0.05). Probit analysis showed that combination of papaya leaves and bitter gourd leaves extract was more effective than Pyrantel pamoate. Conclusions: There were differences of anthelmintic capacity among papaya leaves extract, bitter gourd leaves extract and their combination. The combination of both papaya leaves extract and bitter gourd leaves extract as anthelmintic was more effective than they were alone and also more effective than Pyrantel pamoate. Keywords: Anthelmintic capacity, papaya leaves extract, bitter gourd leaves extract, combination

Pengaruh Ekstrak Biji Kedelai Putih (Glycine max) terhadap Waktu Kematian Cacing Gelang Babi (Ascaris suum) in Vitro ., Pritami; Haryati, Sri; Sakiman, Bambang Sukilarso
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background : White Soy beans (Glycine max) contain saponin and tripsin inhibitor that have been known having anthelmintic effect. This study aimed to determine the effect of White Soy beans extract toward death time of Ascaris suum, in vitro. Methods : The study was a laboratory experimental research using post â test only controlled group design. The subjects were an adult male and female worms (Ascaris suum) that their body length were around 15 cm â 35 cm. The sampling technique used was convenience sampling. The subject was arranged into 6 groups, each group consisted of 4 worms, replication was performed 4 times. NaCl 0.9 % and pirantel pamoat 5 mg/ml was added in the negative control group and in the positive control group, in a row.While white soy beans extract of concentration of 60%, 70%, 80%, and 90% w/v were added to the treatment group. Worms were immersed in 25 ml test solution and incubated at 37Â°C. The subjects were observed every 10 minutes and the number of dead worms was counted. Data were analyzed with linear regression and Pearson correlation. Results : The data of observation showed that total death mean time of Ascaris suum in the negative control and positive control were 2817.5 minutes and 42.5 minutes, while in the treatment group of concentration of 60% , 70% , 80% , and 90%Â w/v of white soy beans extract were 422.5 minutes, 370 minutes, 300 minutes, and 255 minutes, successively. The Pearson correlation test showed very close relationship between variation of the concentration and time of death worms (r = - 0.962). The linear regession test showed significantly (p<0.001) and the regression equation was Y = 2653.400 â 30.340 X and R square was 0.926. Conclusion : White soy beans (Glycine max) extract had effects to the mortality of Ascaris suum in vitro. The most effective white soy beans extract was 90% (w/v) although it was less effective than pirantel pamoat. Keywords : White Soy beans (Glycine max) Extract, Ascaris suum, death time.Â

Pengaruh Ekstrak Wortel (Daucus carota L.) terhadap Waktu Kematian Cacing Ascaris suum, Gooze in Vitro Seno, Aryo; Haryati, Sri; Sakiman, Bambang Sukilarso
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background : Carrots contain quercetin that can be used as antihelmintik. Quercetin is a class of flavonoid glycosides. This study aimed to determine the effect of carrotâs extract (Daucus carota, L.) against the death time of Ascaris suum, Goeze in vitro. Methods :This study was a laboratory experimental research using post-test only controlled group design. Subjects were Ascaris suum, Goeze which were still in active movement. Sampling technique used in this study was convenience sampling. Subjects were arranged into 7 groups, each group consisted of 4 worms, replication was done 4 times. Pyrantel pamoate 5mg/ml and physiological saline were successively added to the test solution of positive control and negative control group, while carrotâs Extract (Daucus carota, Linn) of 70%, 75%, 80%, 85% and 90% were added to the test solution of treatment groups. Worms were soaked in 25 ml of test solution and incubated at 37ÂºC. Observations were done every hour until all the worms died. Data were analyzed using Pearson correlation and linear regression. Results : There was a significant correlation between the concentration variables and time of death of the worms variable (p value of Pearson correlation was 0.000 (<0.050). The higher concentration of the carrot extract was the shorter time of death of worms (Correlation coefficient of concentration variable and time of death of the worms variable was - 0.960). The linear regression equation was Y = 53.775 - 0.609X. Conclusion : The Carrotâs extract (Daucus carota, Linn) had effects on acceleratingÂ the time of death of Ascaris suum, Gooze in vitro. Keywords : Daucus carota, Linn extract, Ascaris suum, GoezeÂ

Perancangan Sistem Informasi Registrasi Dan Pelayanan Akta Kelahiran Pada Dinas Kependudukan Dan Catatan Sipil Kabupaten Kudus Haryati, Sri; Purnama, Bambang Eka
Seruni - Seminar Riset Unggulan Nasional Inoformatika dan Komputer Seruni 2012 - Seminar Riset Unggulan Nasional Informatika dan Komputer
Publisher : Seruni - Seminar Riset Unggulan Nasional Inoformatika dan Komputer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.0809/seruni.v1i1.777

ABSTRAK: Setiap masyarakat pasti ingin mendapatkan pelayanan yang terbaik yang diberikan dari instansi-instansi pelayanan. Dengan melakukan pelayanan yang baik kepada masyarakat maka semakin besar kepercayaan yang diberikan kepada instansi tersebut. Inilah yang menjadi salah satu tugas dari Disdukcapil misalnya di bidang pelayanan akta. Usaha-usahapun telah dilakukan mulai penataan ruang yang nyaman dan fasilitas yang menunjang untuk mempermudahkan petugas melakukan pekerjaannya dengan menerapkan sistem yang telah terkomputerisasi. Sistem komputerisasi yang ada di pelayanan akta lebih terfokus pada proses pembuatan akta kelahiran. Sedangkan untuk pelayanan misalnya pendaftaran akta kelahiran, administrasi pembayaran dan pengambilan akta kelahiran masih dilakukan secara manual. Sehingga bisa menyebabkan antara pendaftaran akta kelahiran dengan administrasi pembayaran tidak sinkron. Kesalahan dalam pengambilan akta kelahiran terkadang juga pernah terjadi.Dari kejadian-kejadian tersebut, peneliti berusaha untuk membuat sistem informasi yang dapat meminimalisir kesalahan yang pernah terjadi. Dengan adanya sistem informasi registrasi dan pelayanan akta kelahiran di disdukcapil Kab. Kudus diharapkan akan membantu dan memudahkan dalam pengolahan data dan pembuatan laporan secara akurat, efektif dan efisien. Kata Kunci : Sistem Informasi, pelayanan ABSTRACT: Each community would like to get the best service provided from service agencies. By doing good service to the community, the greater the credence given to the institution. This has been one of the tasks in areas like service Disdukcapil deed. Business start-usahapun has made a comfortable living arrangements and support facilities to facilitate the officer doing his job by implementing a system that has been computerized. Computerized system that is in deed more services focused on the birth certificate. As for the service such as a birth certificate registration, administration and payment of a birth certificate retrieval is still done manually. So that could lead to the birth certificate of registration with payment is not sikron administration. Birth certificate errors in decision sometimes also terjadi.Dari these events, researchers are trying to create information systems that can minimize the errors that have occurred. With a system of registration information and services on the birth certificate disdukcapil District. Ghost is expected to assist and facilitate in data processing and report accurately, effectively and efficiently. Keywords : Information Systems, service

Perancangan Sistem Informasi Registrasi Dan Pelayanan Akta Kelahiran Pada Dinas Kependudukan Dan Catatan Sipil Kabupaten Kudus Haryati, Sri; Purnama, Bambang Eka
Seruni - Seminar Riset Unggulan Nasional Inoformatika dan Komputer Seruni 2012 - Seminar Riset Unggulan Nasional Informatika dan Komputer
Publisher : Seruni - Seminar Riset Unggulan Nasional Inoformatika dan Komputer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.0809/seruni.v1i1.505

ABSTRAK: Setiap masyarakat pasti ingin mendapatkan pelayanan yang terbaik yang diberikan dari instansi-instansi pelayanan. Dengan melakukan pelayanan yang baik kepada masyarakat maka semakin besar kepercayaan yang diberikan kepada instansi tersebut. Inilah yang menjadi salah satu tugas dari Disdukcapil misalnya di bidang pelayanan akta. Usaha-usahapun telah dilakukan mulai penataan ruang yang nyaman dan fasilitas yang menunjang untuk mempermudahkan petugas melakukan pekerjaannya dengan menerapkan sistem yang telah terkomputerisasi. Sistem komputerisasi yang ada di pelayanan akta lebih terfokus pada proses pembuatan akta kelahiran. Sedangkan untuk pelayanan misalnya pendaftaran akta kelahiran, administrasi pembayaran dan pengambilan akta kelahiran masih dilakukan secara manual. Sehingga bisa menyebabkan antara pendaftaran akta kelahiran dengan administrasi pembayaran tidak sinkron. Kesalahan dalam pengambilan akta kelahiran terkadang juga pernah terjadi.Dari kejadian-kejadian tersebut, peneliti berusaha untuk membuat sistem informasi yang dapat meminimalisir kesalahan yang pernah terjadi. Dengan adanya sistem informasi registrasi dan pelayanan akta kelahiran di disdukcapil Kab. Kudus diharapkan akan membantu dan memudahkan dalam pengolahan data dan pembuatan laporan secara akurat, efektif dan efisien. Kata Kunci : Sistem Informasi, pelayanan ABSTRACT: Each community would like to get the best service provided from service agencies. By doing good service to the community, the greater the credence given to the institution. This has been one of the tasks in areas like service Disdukcapil deed. Business start-usahapun has made a comfortable living arrangements and support facilities to facilitate the officer doing his job by implementing a system that has been computerized. Computerized system that is in deed more services focused on the birth certificate. As for the service such as a birth certificate registration, administration and payment of a birth certificate retrieval is still done manually. So that could lead to the birth certificate of registration with payment is not sikron administration. Birth certificate errors in decision sometimes also terjadi.Dari these events, researchers are trying to create information systems that can minimize the errors that have occurred. With a system of registration information and services on the birth certificate disdukcapil District. Ghost is expected to assist and facilitate in data processing and report accurately, effectively and efficiently. Keywords : Information Systems, service

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