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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Journal of Marine Research Jurnal Kelautan Tropis JURNAL ENGGANO Best Journal (Biology Education, Sains and Technology) Journal of Marine Research

Subagiyo Subagiyo

Department Of Marine Science, Faculty Of Fisheries And Marine Science, Universitas Diponegoro

Author-ID : 2958025

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Public Health Other

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Kontaminasi Arsen, Merkuri dan Magnesium pada Air Laut Sedimen dan Anadara inaequivalvis (Mollusca: Bivalvia, Bruguiera, 1792) di Perairan Brebes, Indonesia Chrisna Adhi Suryono; Sugeng Widada; Baskoro Rochaddi; Subagiyo Subagiyo; Wilis Ari Setyati; Endang Sri Susilo
Jurnal Kelautan Tropis Vol 21, No 2 (2018): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Contamination of Arsen, Mercury and Magnesium in the Seawater, Sedimen and Anadara inaequivalvis (Mollusca: Bivalvia, Bruguiera, 1792) in Brebes, Indonesia Brebes coastal areas have been developed into industrial areas and human settlement. It has been causing effect on coastal environmental. Heavy metal is one of many source of pollution in coastal environmental. In order to assess As, Hg and Mg contaminant on the Brebes coast, Central Java, samples of marine water, sediment and Anadara inaequivalvis specimens were collected for analyzed by Inductively Coupled Plasma-Mass Spectrometer (ICPMS). The result showed that the heavy metal As, Hg and Mg were found in the sea water, sediment, and in the tissue of A. inaequivalvis. The concentration of Mg was the highest compared to As and Hg in all samples. Meanwhile As and Hg had the lowest concentration. We concluded that Brebes coastal areas has been contaminated by heavy metals. The bio-ability of bivalves to accumulate heavy metals depends on many factors such as geochemical and biological factors. Pesisir Brebes telah berkembang menjadi daerah industri dan tempat tinggal. Hal tersebut tentunya akan memberi dampak pada lingkungan pesisir. Logam berat perupakan salah satu penyebap pencemaran di lingkungan pesisir. Untuk mengetahui kontaminasi As, Hg dan Mg di pesisir Brebes, maka beberapa sampel air laut, sedimen dan kerang A. inaequivalvis dianalisa menggunakan ICPMS. Hasil penelitian menunjukan bahwa logam berat As, Hg dan Mg ditemukan dalam air laut, sedimen dan kerang A. inaequivalvis. Konsentrasi Mg menunjukan nilai yang paling tinggi di semua sampel yang dianalisa. Sedangkan konsentrasi As dan Hg menunjukan nilai yang rendah secara berurutan. Hal ini dapat disimpulkan bahwa pesisir Brebes talah terkontaminasi oleh logam berat. Kemampuan bivalvia unuk mengakumulasi logam tergantung banyak faktor seperti kondisi geokimiawi dan biologis.

Fluktuasi Fitoplankton pada Kawasan Konservasi Rajungan Perairan Betahwalang Demak Elsa Lusia Agus; Rudhi Pribadi; Subagiyo Subagiyo
Jurnal Kelautan Tropis Vol 22, No 2 (2019): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Phytoplankton is an organism that had an effect on life waters and can be used as the parameters in monitoring water quality. Distribution of phytoplankton in the waters can be for biomonitoring which can be used to make protection zone. This study will examine the distribution and composition of phytoplankton in The crab conservation area in Betahwalang Waters, Demak. This research divided into three stations in estuaries and the sea s. Each sampling was done once a month. Samples were taken using a plankton net. The results found three classes of phytoplankton, namely Bacillariophyceae class, Cyanophyceae class and Dinophyceae class. The number of genera found in the river area (37 genera), at the estuary station as much (38 genera) and the sea station as much (31 genera). Fluctuation in phytoplankton abundance at each station is quite varied. The abundance of phytoplankton in January was 1.030.400 cells/L, in February it was 936.800 cells/L, and in March it was 643.600 cells/L. The highest abundance occurs in the estuary area, then the river and the lowest in the sea. The value of species diversity index (H ') is in the range of 2.57-3.03. Uniformity index values range from 0.86 to 0.94. The dominance index from 0.06 to 0.14. Factors affecting the abundance and dominance of phytoplankton are water conditions and the rainy season. Fitoplankton merupakan organisme yang mempunyai pengaruh besar terhadap kehidupan di suatu perairan baik langsung maupun tidak langsung dan dijadikan sebagai salah satu parameter dalam pemantauan kualitas perairan.Fitoplankton di perairan dapat dijadikan suatu ukuran bahwa Kawasan tersebut dapat dijadikan sebagai zona perlindungan biota.Penelitian ini mengkaji fluktuasi fitolankton pada Kawasan konservasi rajungan di Perairan Betahwalang, Demak. Penelitian ini dilakukan pada 3 stasiun yang terletak disungai, muara sungai, dan laut. Pengambilan sampel dilakukan setiap bulan selama tiga bulan menggunakan planktonnet. Hasil penelitian ditemukan 3 kelas yaitu kelas Bacillariophyceae, Cyanophyceae dan Dinophyceae.Jumlah genus yang ditemukan pada area sungai, muara sungai dan laut berturut-turut 37 genus, 38 genus dan 31 genus. Fluktuasi kelimpahan fitoplankton di setiap stasiun cukup bervariasi.Kelimpahan fitoplankton pada bulan Januari sebesar 1.030.400 sel/L, pada bulan Februari sebesar 936.800 sel/L, dan pada bulan Maret sebesar 643.600 sel/L. Kelimpahan tertinggi terjadi pada daerah muara, kemudian sungai dan terendah di laut. Sedangkan nilai indeks keanekaragaman jenis (H’) berada pada kisaran 2,57-3,03. Nilai indeks keseragaman berkisar 0,86-0,94. Sedangkan indeks dominasi berkisar 0,06-0,14. Faktor yang mempengaruhi kelimpahan dan dominasi fitoplankton adalah kondisi perairan dan musim hujan.

Skrining Dan Seleksi Bakteri Simbion Spons Penghasil Enzim Ekstraseluler Sebagai Agen Bioremediasi Bahan Organik Dan Biokontrol Vibriosis Pada Budidaya Udang Wilis Arii Setyati; Ahmad Saddam Habibi; Subagiyo Subagiyo; Ali Ridlo; Nirwani Soenardjo; Rini Pramesti
Jurnal Kelautan Tropis Vol 19, No 1 (2016): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

The rapid cultivation of aquaculture with the application of intensive systems in recent years, has raised problems in the form of a decrease in the carrying capacity of ponds for the life of aquaculture organisms. The further impact caused is the occurrence of a series of diseases and damage to environmental conditions. Anticipatory measures through the application of bioremediation are solutions to prevent more serious damage. In the bioremediation process, enzymes play the role of catalysts that accelerate biochemical reactions in ponds of soil and water. Bacteria associated with sponges have various bioactive compounds that can inhibit the growth of pathogenic bacteria. The purpose of this study is isolation and screening of bacteria associated with sponges that have extracellular enzyme activity (proteolytic, amylolytic, cellulolytic, and lipolytic) and antibacterial activity, as well as knowing the interaction of antagonism properties among the best isolates, and phenotypic identification of bacterial species. This research was conducted in 4 stages: sample collection, isolation, selection, and identification. The results showed that the total sponge symbiotic bacteria obtained were 15 isolates with the potential of 15 proteolytic isolates, 12 amylolytic isolates, 12 lipolytic isolates, and 4 cellulolytic isolates. There were 10 isolates having antibacterial activity against Vibrio harveyi and 2 isolates had antibacterial activity against Vibrio alginolyticus. The best bacterial isolates SP.1.3, SP.5.1, and SP.5.3 have no antagonistic activity between the three. Phenotypic identification of 3 isolates alleged that isolates SP.1.3, SP.5.1, and SP.5.2 were identified as Bacillus sp., Acinetobacter sp., And Pseudomonas sp.Pesatnya kegiatan budidaya perikanan dengan penerapan sistem intensif dalam beberapa tahun terakhir, telah memunculkan permasalahan berupa penurunan daya dukung tambak bagi kehidupan organisme budidaya. Dampak lanjut yang ditimbulkan adalah terjadinya serangkaian penyakit dan kerusakan kondisi lingkungan. Langkah antisipatif melalui penerapan bioremediasi merupakan solusi untuk mencegah kerusakan yang lebih serius. Dalam proses bioremediasi, enzim memainkan peran katalis yang mempercepat reaksi biokimia di kolam tanah dan air. Bakteri yang berasosiasi dengan spons memiliki beragam senyawa bioaktif yang dapat menghambat pertumbuhan bakteri patogen. Tujuan dari penelitian ini adalah isolasi dan skrining bakteri yang berasosiasi dengan spons yang memiliki aktivitas enzim ekstraseluler (proteolitik, amilolitik, selulolitik, dan lipolitik) dan aktivitas antibakteri, serta mengetahui interaksi sifat antagonisme antar isolat terbaik, dan identifikasi spesies bakteri secara fenotipik. Penelitian ini dilakukan dalam 4 tahap: koleksi sampel, isolasi, seleksi, dan identifikasi. Hasil penelitian menunjukkan bahwa total bakteri simbion spons yang diperoleh 15 isolat dengan potensi 15 isolat proteolitik, 12 isolat amilolitik, 12 isolat lipolitik, dan 4 isolat selulolitik. Terdapat 10 isolat memiliki aktivitas antibakteri terhadap Vibrio harveyi dan 2 isolat memiliki aktivitas antibakteri terhadap Vibrio alginolyticus. Isolat bakteri terbaik SP.1.3, SP.5.1, dan SP.5.3 tidak memiliki aktivitas saling antagonis antar ketiganya. Identifikasi fenotipik dari 3 isolat diduga bahwa isolat SP.1.3, SP.5.1, dan SP.5.2 diidentifikasi sebagai Bacillus sp., Acinetobacter sp., dan Pseudomonas sp.

Meta-analysis of Indonesian Octopus laqueus Kaneko & Kubodera 2005 (Cephalopoda: Octopodidae) using Mt-DNA COI as Genetic Marker Nenik Kholilah; Norma Afiati; Subagiyo Subagiyo; Retno Hartati
Jurnal Kelautan Tropis Vol 24, No 1 (2021): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v24i1.10190

O. laqueus was first discovered not long ago in 2005 in the Ryuku Islands, Japan. Its geographical distribution and molecular identification are therefore still rarely. Nucleotide sequences based on mt-DNA COI for O. laqueus that have been uploaded in the GenBank until before this study was carried out were only six sequences. Since DNA barcoding of mt-DNA COI has some advantageous characteristics, this study aimed to analyse the genetic difference of Indonesian O. laqueus to the data available in the GenBank. Samples were collected in 2019 - 2020 from Karimunjawa (n=16) and Bangka-Belitung (n=2). The mt-DNA COI was extracted using 10% chelex methods, PCR amplified using Folmer’s primer and sequenced in Sanger methods. Pairwise alignment and genetic distance were carried out in MEGA-X, whereas the phylogenetic tree was reconstructed using Bayesian methods. BLAST identification resulted in 685 bp with a range of 92,07-99,24 percentages of identity. The genetic mean pair-wise distances within-clade were 0,002 and 0,006, whilst the distance between the clade was 0.0883. Combining the suggestion with the ITF current, it is concluded that O. laqueus taken from Karimunjawa raised from the same species as those in Malaysia (MN711655) and Japan (AB302176). Specimens from Bangka-Belitung were suggested came from different species, as they were separated into the second clade by 8.83%. One single sample from Japan (AB430543) which laid outside the two clades by 11.63%-11.38% was also suggested to represent a different species. Overall, this study opens to various further studies on O. laqueus using other loci of genetic markers.

Identifikasi dan Analisis Filogenetik Portunus trituberculatus Dari Perairan Cirebon Menggunakan Barkode Gen COI Mitokondrial Subagiyo Subagiyo; Retna Handayani; Rahayu Rahayu; Triandala Mada Sibero
Jurnal Kelautan Tropis Vol 21, No 2 (2018): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Phylogenetic Identification and Analysis Portunus trituberculatus from Cirebon Coast Using the COI Barcode Mitochondrial Portunus trituberculatus spesimen from Cirebon coast were successfully identified using mitochondrial DNA cytochrome c oxydase subunit I (COI) genes. Analysis of haplotype distribution of P. trituberculatus along with the same species from China, Korea, India and the Philippines obtained from NCBI gene banks resulted 17 haplotypes from 25 specimens. Haploid diversity was 0.943 + 0.031 and nucleotide diversity was 0.04821 + 0.0139. The Cirebon specimen is in separated haplotipe from the others. The results of phylogenetic analysis showed that the 25 specimens were clustered into 3 clusters in 2 different lineages with percentages genetic distance were 12.76%, 14.24% and 14.33% respectively. The genetic distance within each cluster ranges from 0 - 2.92%. The Cirebon crab specimen is in the same cluster as the Philippine specimen with 1% genetic distance. Spesimen rajungan Portunus trituberculatus dari perairan Cirebon berhasil diidentifikasi menggunakan gen mitochondrial DNA cytochrome c oxidase subunit I (COI). Analisis distribusi haplotipe dengan data P trituberculatus yang berasal dari China, Korea, India dan Filipina yang diperoleh dari data genebank NCBI didapatkan 17 haplotipe dari 25 spesimen, dengan keragaman haploid 0,943 +0.031 dan keragaman nukleotida 0,04821+0.0139. Spesimen Cirebon merupakan haplotipe yang terpisah dari yang lainnya. Hasil kajian filogenetik menunjukkan 25 spesimen mengelompok ke dalam 3 kluster dari 2 garis keturunan yang berbeda dengan jarak genetik berturut turut 12,76 %, 14,24 % dan 14,33 %. Jarak genetik di dalam masing-masing kluster berkisar antara 0 – 2,92 %. Spesimen rajungan Cirebon berada pada garis keturunan dan kluster yang sama dengan spesimen Filipina dengan jarak genetik 1%.

Preliminary Study of Polysaccharide and Oligosaccharide Alginate (AOS) as Prebiotic of Probiotic Bacteria Ervia Yudiati; Subagiyo Subagiyo; Muhammad Salauddin Ramadhan Djarod
Jurnal Kelautan Tropis Vol 23, No 2 (2020): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v23i2.7674

Synbiotics are related to pre and probiotic mixture which work synergically and provide the intestine microbial balance in organisms. This study aims to evaluate the potency of alginate polysaccharide and oligosaccharide (AOS) as prebiotic to probiotic bacteria originally from a commercial yogurt. Probiotic bacteria were plated to MRSA and incubated in 24 hrs. Over five isolated bacteria, three probiotic isolate bacteria were defined positively(C11; C14 and C15). The prebiotic test was done by culturing the isolate C14 to MRSB and incubated 24 hrs. After 24 hrs, all the MRSB liquid culture became turbid, showed a positive effect, except the MRSB culture without any alginate enrichment. Probiotic bacteria from MRSB were then plated into MRSA for Total Plate Count (TPC) test and incubated. TPS revealed that 0.1 mg/mL enriched AOS media resulted the highest number of probiotic bacteria (1.193 x 105 CFU/mL) compared to enriched AOS and 0.05 mg AOS/mL media (6 x 103 CFU/mL). These results have proven a high ability to develop AOS as prebiotic.

Potensi Ekosistem Mangrove Sebagai Sumber Bakteri Untuk Produksi Protease, Amilase Dan Selulase Subagiyo Subagiyo; Muhammad Salauddin Ramadhan Djarod; Wilis Ari Setyati
Jurnal Kelautan Tropis Vol 20, No 2 (2017): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

The mangrove ecosystem is a productive ecosystem. The production and accumulation of organic matter on the floor of the mangrove ecosystem allows this region to be rich in microbials that are potential to be developed as a source of a wide range of extra cellular enzymes. In this research, bacteria were isolated and used as the source of enzyme. The bacteria were isolated from the existing mangrove sediments on the north coast of Semarang, Central Java. The bacterial potential as a source of protease, amylase and cellulase is done semiquantitatively using well diffusion agar method. The results obtained 18 bacteria isolates which were all capable to produce amylase and cellulase, but only 7 isolates were able to produce proteases. Five isolates had amylase activity above 300 mm2 / ml, three isolates with protease activity above 300 mm2 / ml and 2 isolates with cellulase activity of 225.40 and 204.50 mm2 / ml. Seven isolates are capable of producing amylase, protease and cellulase. The results showed that mangrove ecosystem is a potential source of microbes to produce enzymes protaese, amylase and cellulase and also possible for various other extracellular enzymes. Ekosistem mangrove merupakan ekosistem produktif. Produksi dan akumulasi bahan organik di lantai ekosistem mangrove memungkinkan daerah ini kaya akan mikrobia yang potensial untuk dikembangkan sebagai sumber berbagai macam enzim ekstra seluler. Pada penelitian ini dilakukan isolasi bakteri sebagai sumber enzim. Bakteri diisolasi dari sedimen mangrove yang ada di pantai Utara Semarang, Jawa Tengah. Pengujian potensi bakteri ini sebagai sumber protease, amilase dan selulase dilakukan secara semikuantitatif menggunakan metode well diffusion agar. Hasil penelitian mendapatkan 18 isolat bakteri yang seluruhnya mampu memproduksi amylase dan selulase, tetapi hanya 7 isolat yang mampu memproduksi protease. Lima isolat memiliki aktivitas amylase diatas 300 mm2/ml, tiga isolat dengan aktivitas protease diatas 300 mm2/ml dan 2 isolat dengan aktivitas selulase yaitu sebesar 225,40 dan 204,50 mm2/ml. Tujuh isolat yang mampu menghasilkan amylase, protease dan selulase. Hasil penelitian menunjukkan bahwa ekosistem mangrove merupakan sumber potensial mikrobia untuk menghasilkan enzim protaese, amylase dan selulase serta juga dimungkinkan untuk berbagai macam ensim ekstraseluler lainnya.

Morfometri, Pemijahan, dan Indeks Kematangan Gonad Teripang Komersial di Perairan Pulau Nyamuk, Karimunjawa Mustagfirin Mustagfirin; Diah Permata Wijayanti; Subagiyo Subagiyo
Jurnal Kelautan Tropis Vol 24, No 3 (2021): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v24i3.11696

Sea cucumbers are marine organisms that live in coral reef ecosystem habitats and their associations. Sea cucumbers have been used by communities as food, cosmetics and traditional medicine in various countries. The benefits and high economic value of sea cucumbers make fisheries activities occured massively and continuously. Sea cucumbers were large commodity from Indonesian waters. However, until now it has not received proportional attention. The lack of information on morphometry, spawning in various regions has made the commodity of sea cucumbers decreased. This research was conducted to determine the character of commercial sea cucumber commodities in Pulau Nyamuk, to the occurrence of spawning based on the moon phase. This research was conducted in September - November 2020. This research method uses an analysis approach of gonadal maturity index in determining the spawning of sea cucumbers. The results showed that commercial sea cucumbers in Pulau Nyamuk were Pisang (Holothuria sp.) and Gamat (Stichopus sp.) Sea cucumber. This sea cucumber weighs 300-690 grams wet. Sea Cucumbers, Pisang and Gamat tend to have high IKG values in the new moon phase with IKG index values >10%. Teripang merupakan organisme laut yang hidup pada habitat ekosistem terumbu karang dan asosiasinya. Teripang telah dimanfaatkan oleh masyarakat di sekitar pantai sebagai bahan makanan, kosmetik serta obat tradisional di berbagai negara. Manfaat serta nilai ekonomi teripang yang tinggi membuat aktivitas pemburuan terjadi secara masif dan terus menerus. Teripang juga merupakan komoditas besar yang berasal dari Perairan Indonesia. Namun hingga saat ini belum memperoleh perhatian yang proporsional. Minimnya informasi mengenai morfometri, pemijahan di berbagai daerah membuat komoditas teripang turun secara drastis. Penelitian ini dilakukan untuk mengetahui karakter komoditas teripang komersial di Pulau Nyamuk, hingga terjadinya pemijahan berdasarkan fase bulan. Penelitian ini bulan September - November 2020 di Perairan Pulau Nyamuk Kecamatan Karimunjawa, Kabupaten Jepara. Metode penelitian ini menggunakan pendekatan analisis indeks kematangan gonad dalam menentukan pemijahan teripang. Hasil penelitian menunjukkan bahwa teripang komersial di Pulau Nyamuk adalah jenis teripang Pisang (Holothuria sp.) dan Gamat (Stichopus sp.). Teripang ini mempunyai berat 300-690 gram basah. Teripang Pisang dan Gamat cenderung mempunyai nilai IKG yang tinggi pada bulan baru dengan nilai indeks IKG >10%.

AKTIVITAS ANTIOKSIDAN EKSTRAK RUMPUT LAUT Halimeda macroloba DARI PANTAI TELUK AWUR, JEPARA, JAWA TENGAH Ahmad Fadhil Muzaki; Wilis Ari Setyati; Subagiyo Subagiyo; Rini Pramesti
JURNAL ENGGANO Vol 3, No 2
Publisher : Universitas Bengkulu

Antioksidan yang dihasilkan oleh tubuh berfungsi sebagai mekanisme perlindungan terhadap serangan radikal bebas memiliki jumlah yang terbatas. Antioksidan eksogen yang aman dan mudah diperoleh adalah antioksidan dari bahan alam seperti Halimeda macroloba yang mengandung senyawa bioaktif meliputi fenol, klorofil a dan b, serta karotenoid. Metode penelitian yang digunakan adalah deskriptif eksploratif. H. macroloba diperoleh dari Pantai Teluk Awur - Jepara dicuci bersih dengan air tawar dan diblender. Sampel dimaserasi pada pelarut metanol, etil asetat, dan n-heksan, serta diuapkan dengan rotary evaporator. Aktivitas antioksidan ditentukan dari nilai IC50. Nilai ini diperoleh dari persamaan kurva regresi linier pada nilai inhibisi yang terdeteksi dari nilai absorbansi DPPH murni terhadap pengaruh ekstrak H. macroloba yang diukur pada panjang gelombang 517 nm. Kadar total fenol diuji menggunakan larutan Folin-Ciocalteu dengan asam galat sebagai larutan standar yang diukur pada panjang gelombang 725 nm. Kadar klorofil a, b, dan karotenoid diukur pada panjang gelombang 663 nm, 646 nm, dan 470 nm. Hasil penelitian menunjukkan nilai IC50ekstrak metanol sebesar 38,57 ppm, etil asetat 1567,27 ppm dan n-heksan 259,34 ppm. Kadar total fenol pada masing-masing ekstrak adalah 87,10; 140,78 dan 102,24 (mg GAE/g berat basah sampel), kadar klorofil a sebesar 25,56; 28,44 dan 0,41 (mg/g berat basah sampel), kadar klorofil b sebesar 42,10; 41,94 dan 0,61 (mg/g berat basah sampel), dan kadar karotenoid sebesar 97,92; 85,68 dan 2,34 (µ mol/g berat basah sampel). Nilai IC50 dari ekstrak metanol termasuk kategori aktivitas antioksidan sangat kuat sedangkan ekstrak etil asetat dan n-heksan termasuk kategori aktivitas antioksidan sangat lemah.

Aktifitas Antibakteri Isolat Bakteri Asam Laktat Saluran Pencernaan Kuda Laut (Hippocampus kuda Bleeker, 1852) Terhadap Vibrio harveyi Fionica May Sandi; Subagiyo Subagiyo
Jurnal Kelautan Tropis Vol 25, No 2 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i2.12368

Seahorses (Hippocampus kuda) have high economic value in global trade. Therefore, to meet market demand, cultivation efforts have been carried out. Vibriosis caused by Vibrio harveyi is a disease that is often reported to affect seahorses. In this study, lactic acid bacteria were isolated from the digestive tract of seahorses, and were selected based on their antibacterial activity against V, harveyi. Antibacterial activity test was carried out by agar diffusion method. Isolates with antibacterial activity were identified based on their morphological and biochemical characteristics. The results of this study obtained 3 (three) LAB isolates that were active against V. harveyi from a total of 45 isolates, namely SPKL 34, SPKL 08 and SPKL 58. The SPKL 34 isolate had the highest antibacterial activity. The three isolates were identified as lactobacillus sp Kuda laut (Hippocampus kuda) memiliki nilai ekonomi tinggi sebagai salah satu jenis komoditas perdagangan global. Oleh karena itu untuk memenuhi permintaan pasar diantaranya telah dilakukan upaya budidaya. Vibriosis yang disebabkan oleh Vibrio harveyi merupakan penyakit yang sering dilaporkan menyerang Kuda Laut. Pada penelitian telah dilakukan pencarian jenis-jenis bakteri asam laktat (BAL) yang berasal dari saluran pencernaan Kuda Laut yang memiliki aktivitas antibakteri terhadap V. harveyi. Uji aktivitas antibakteri dilakukan dengan metode difusi agar. Isolat yang memiliki aktivitas antibakteri diidentifikasi berdasarkan karakteristik morfologi dan biokimia. Hasil penelitian ini memperoleh 3 (tiga) isolat BAL yang aktif terhadap V. harveyi dari total 45 isolat yaitu SPKL 34, SPKL 08 dan SPKL 58. Isolat SPKL 34 memiliki aktivitas yang paling tinggi yaitu dengan rerata luas zona hambat sebesar 4,14±0,60 mm. Hasil identifikasi menunjukkan ketiga isolat merupakan Lactobacillus sp. dengan tingkat kemiripan >80%.

Co-Authors Adi Santoso Agus Sabdono Agus Trianto Agus Trianto Ahmad Fadhil Muzaki Ahmad Saddam Habibi Aiyen Tjoa Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Ridlo Ambariyanto Ambariyanto Aninditia Sabdaningsih Antonius Budi Susanto Bambang Yulianto Baskoro Rochaddi Chrisna Adhi Suryono Chrisna Adi Suryono Delianis Pringgenies Diah Permata W Diah Permata Wijayanti Dwi Haryanti Dwi Haryo Ismunarti Elis Indrayanti Elsa Lusia Agus Endang Sri Susilo Erni - Martani Ervia Yudiati Feri Setiawan Fifi Nur Hidayah Fionica May Sandi Gagas Tri Pamungkas Haniâ€™atun Nurfajriyah Henna Rya Abdurachim Husna El Iksiroh Husna Hanifah Irwani Irwani Isai Yusidarta Ita Riniatsih Jusup Suprijanto Kharisma Firdaus Linggarjati Luh Putu Puspita Dewanti Mada Triandala Sibero Marthin Ricky Sipayung Mirsa Septiana Mutik Mostafa Imhmed Ighwerb Muhammad - Zainuddin Muhammad Aviv Arsya Irnantyanto Muhammad Juli Hendra Putra Muhammad Salauddin Ramadhan Djarod Mustagfirin Mustagfirin Mustalafin Mustalafin Nenik Kholilah Nenik Kholilah Nirwani Soenardjo Norma Afiati Novita Thea Puspita Langit Nur Taufiq Syamsudin Putra Jaya Taufiq Syamsudin Putra Jaya Nuril Azhar Nuril Azhar Ocky Karna Radjasa Rabia Alghazeer Radhian Wikanarto Widodo Rahayu Rahayu Refi Sekarwardhani Retna Handayani Retno Hartati Retno Murwani Ria Azizah Ria Azizah Ria Azizah TN Ria Azizah Tri Nuraini Ria Azizah Tri Nuraini Ria Azizazh Tri Nuraeni Rignolda Djamaludin Rini Pramesti RINI PRAMESTI Rudhi Pribadi Sebastian Margino Sebastian Margino Sebastian Margino Sonny Rieldo Damanik Sri Devi Simanjuntak Sri Lintang Artono Sugeng Widada Suryono Suryono Sutrisno Anggoro Sylvia Sari Indah Dongoran Titis Buana Triyanto - - Triyanto Triyanto Triyanto Triyanto Triyanto Triyanto Widianingsih Widianingsih Wilis A Setyati Wilis A Setyati Wilis A Setyati Wilis Ari Setyati Wilis Ari Setyati Yohanes Oktaviaris Yolanda Christia Simanjuntak

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