Article Per Year (5 Year)
p-Index From 2019 - 2024
1.463
P-Index
This Author published in this journals
All Journal BALI MEDICAL JOURNAL (BMJ) journal of internal medicine International Journal of Biosciences and Biotechnology Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana Buletin Udayana Mengabdi INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Jurnal Veteriner Jurnal Biologi Udayana Microbiology Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Kesmas: Jurnal Kesehatan Masyarakat Nasional ISM (Intisari Sains Medis) : Jurnal Kedokteran Journal of Global Pharma Technology
I NYOMAN MANTIK ASTAWA
Laboratorium Virologi, Departemen Penyakit Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali-Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

Published : 63 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 5 Documents
Search
Journal : BALI MEDICAL JOURNAL (BMJ)

 1 
EPIDEMIOLOGICAL AND MOLECULAR ANALYSIS OF TOXOPLASMA GONDII IN FAECAL SAMPLES OF CATS OBTAINED FROM HOUSE OF MATERNAL IN BALI Subrata, I M.; Suryadhi, N. T.; Astawa, N. Mantik; Damriyasa, I M.
BALI MEDICAL JOURNAL Vol 4 No 2 (2015)
Publisher : BALI MEDICAL JOURNAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (617.401 KB)

Abstract

Background: toxoplasmosis is a zoonotic-parasite disease caused by T.gondii that spread in many countries. Recently, this disease is one of the major public health problems associated with severe enocomical and social impacts such as miscarriages, hydrocephalus, blindness and mental retardations. The human parastic infection are closely related with the presence of infected cats around their houses, in which the animals are the definitive host releasing the parasites into the environment via their feces. The aim of this research was to determine the correlation between morphological prevalence and molecular detection of T.gondii in cats feces. Methods: detection of molecular was carried out by applying microscopic and a specific polymerase chain reaction (PCR). A case-control study using 40 maternal with positive serology of T. gondii and 40 maternal with negative serology were selected by purposive sampling method. Similarly, 80 faecal sample of cats were also obtained from each house of the women. The major risk factors considered as the important rule for human toxoplasmosis such as age, education, occupation, contact with soil, cat’s age, un-owned cats and oocyst-positive feces were determined by questionnaire. Results: The epidemiological analysis showed that the highest risk factor was oocyst-positive feces (OR= 8.143; p= 0.003), followed by education (OR= 3.414; p=0.045), contact with soil (OR= 2.255; p= 0.073), un -owned cats (OR= 2.25; p= 0.210), age (OR=2.23; p=0.074), occupation (OR= 1.556; p= 0.348) and cat’s age (OR= 1.138; p= 0.799). However, the pregnant women with serologically positive to T. gondii had a very high probability of miscarriages (OR= 18.857; p= 0.0001) compared to whom with serologically negative. Moreover, logistic regression model analysis revealed that only oocyst-positive feces and education contributed a significant factor in causing toxoplasmosis among pregnant women. Microscopic observation of 80 faecal samples indicated that only 14 (17.5%) contained typical oocyst of T. gondii. All of the 8 oocyst of T. gondii positive-faecal samples were further confirmed with PCR, and we found that 4 (50%) were positive. Conclusion: This study concluded that the presence of oocyst-positive feces in the environment in conjuction with education level were considered to play significant contribution to the accident of toxoplamosis in pregnant women.
Lactose-Astaxanthin Increases Green Jungle Fowl’s Sperm Motility and Reduces Sperm DNA Fragmentation During 5o Celsius Storage Bebas, Wayan; Pemayun, Tjok Gede Oka; Damriyasa, I Made; Astawa, I Nyoman Mantik
BALI MEDICAL JOURNAL Vol 4 No 3 (2015)
Publisher : BALI MEDICAL JOURNAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (253.155 KB)

Abstract

Background: Good quality of semen is required for artificial insemination technology in ex-situ conservation efforts of green jungle fowl. This study was aimed to investigate semen quality of green jungle fowl during storage at 5oC for 48 hours with the addition of combination lactose astaxanthin in egg yolk phosphate dilution. Method: The semen used in the study was collected from eight healthy male green jungle fowls by using massage techniques. The semen quality was analyzed with macroscopic and microscopic examinations. The semen was diluted with egg yolk phosphate with the addition of 0.6% lactose, 0,004% astaxanthin and combination 0.6% Laktose-0,004% astaxanthin, and was stored at 5oC for 48 hours. Following 48-hour treatment, the semen quality was evaluated based on its progressive motility, and DNA fragmentation. Data were firstly analyzed by using analysis of variance (ANOVA), and were then proceeded by using Duncan Multiple Range test. Results: The results showed that the progressive motilities of semen diluted in 0.6% lactose combined with astaxanthin 0.004% %, (79,66 + 1.50%) was significantly higher than those diluted in 0.6% lactose (66,77 + 2.16%,) and in astaxanthin 0.004% (68,11 + 3.01 %). The DNA fragmentation of semen diluted inn 0.6% lactose combined with astaxanthin 0.004% %, (7,55 + 1,66%) was significantly lower than those diluted in 0.6% lactose (12,33 + 1,93%) and in astaxanthin 0.004% (13,55 + 1,81%). Conclusions: In conclusion, the combination of l 0.6% lactose -astaxanthin 0.004% showed the best results for progressive motility, and DNA fragmentation.
BINGE ALCOHOL ADMINISTRATION ON PREGNANT RATS RESULTS IN DECREASING OF INSULIN LIKE GROWTH FACTOR-1 AND ALDEHYDE DEHYDROGENASE, INCREASING APOPTOSIS INDEX, AND FETAL ALCOHOL SYNDROME IN OFFSPRINGS. Suherman, Sutjahjo; Soetjiningsih, S.; Suastika, Ketut; Astawa, I Nyoman Mantik
BALI MEDICAL JOURNAL Vol 5 No 1 (2016)
Publisher : BALI MEDICAL JOURNAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.586 KB)

Abstract

Background: Addiction of alcoholic beverage by early pregnancy women results in fetal alcohol syndrome of her baby. This study aims to investigate fetal alcoholic syndrome due to binge alcoholic drinking by the early pregnant of wistar rat. Methods: This is an experimental study applying posttest only control group design. Wistar Rats were in preconditioning for pregnancy and divided into two groups, i.e. one group was fed with normal feeding and the other group was fed with normal feeding and 40% of ethanol. The off spring then were observed and divided into three groups, i.e. normal fetal, normal fetal from the mother fed with ethanol, and fetal alcoholic syndrome. Insulin like growth factor (IGF-1), aldehyde dehydrogenase (ALDH), apoptosis index, pathology of their brain and heart were observed. The different of all these parameters were then compared by applying one way anova, and considered significant at p < 0.05. Results: In this study we found that there were fetals alcoholic syndrome (FAS) due to the mother of the Wistar Rat fed with ethanol during their pregnancy. There were also a significant different of IGF-1, ALDH, apoptosis index between these three groups (p < 0.05), i.e. normal baby, normal fed with ethanol, and FAS. IGF-1 for these three groups were 56.59±0.52 ng/ml, 55.17±2.41 ng/ml, and 36.64±4.86 ng/ml, respectively. ALDH for the groups were 21.41±2.38 ng/ml, 21.16±4.77 ng/ml, and 17.05±2.68 ng/ml, respectively. Their brain apoptosis indexes were 4.56±0.78, 4.58±1.17, and 7.86±1.31, respectively. Heart apoptosis indexes were found 2.81±1.18, 5.36±1.37, and 7.50±1.43, respectively. Conclusion: Binge alcohol drinking during pregnancy of Wistar Rat results in FAS and identified by decrease of IGF-1, ALDH and increase of brain apoptosis index and heart apoptosis index of the off spring.
Anti-Inflammatory Effect of Red Piper Crocatum Leaves Extract Decrease TNF-? and IL-6 Levels in Wistar Rat with Atherosclerosis Wahjuni, Sri; Wita, I W.; Astawa, I N. Mantik
BALI MEDICAL JOURNAL Vol 5 No 2 (2016)
Publisher : BALI MEDICAL JOURNAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (507.783 KB)

Abstract

Background: This research aims to find a cure for anti-inflammation, based on the utilization of red piper crocatum. The research was started with descriptive study to explore active components of red piper crocatum leaf and followed by experimental study to investigate red piper crocatum activity of the leaf extract in anti-inflammation induced Wistar rat. In this research observed three dominant components: caryophyllene bicyclo [5.2.0] none, 2 methylene-4,8,8-trimethyl-4-vinyl; phytol; 5,9-propano-5H- benzocycloheptene, 6,7,8,9-tetrahydro-7,11-bis(methylene); 4,4-ethynedioxy-2-hexadecen-15-15 olide 1,4,9-trioxaspiro [4,15] eic os-6-en-8-one, 10 methyl; 1H-1,2,4-triazole-5(H)-thione,4-allyl-3-(3-furyl); Benzofuran,2,3-dihydro-2-methyl-7-phenyl which are possibly active to inhibit anti-inflammation to atherosclerosis. Bad eating habits also can cause various health problems, such as obesity, dyslipidemia, inflammation to atherosclerosis. This study was conducted to investigate of red piper crocatum leaves extract as an anti-inflammation through decrease of biochemistry markers TNF-? and IL-6 levels. Method: This is a true experimental with randomized pre-test and post-test control group design, using 50 Wistar rats that are divided into 5 groups: control group using 0 mg/kg B red piper crocatum leaves extract, treatment group 1 using 50 mg/kg BW red piper crocatum leaves extract, treatment group 2 using 100 mg/kg BW red piper crocatum leaves extract, treatment group 3 using 150 mg/kg BW red piper crocatum leaves extract, and treatment group 4 200mg/kg BW red piper crocatum leaves extract. Results: It was observed that intake of 150 mg/B red piper crocatum leaves extract results in the highest significance decrease of 45.63% of TNF-? levels from (28.62 ± 1.25 to 15.56 ± 7.20 ?g/mL) and a significance decrease of 15.42% of IL-6 level from (134.64 ± 1.98 to 113.87 ± 4.30 ?g/mL). Conclusion: It can be concluded that intake of red piper crocatum leaves extract acts as antiinflammation for Wistar rats with atherosclerosis through decrease of TNF-? and IL-6 levels. Further research is required to determine whether the application of red piper crocatum leaves extract on human will result in similar effects of anti-inflammation.
COMPARATIVE IMMUNE RESPONSES OF CHILDREN AFTER INTRADERMAL AND INTRAMUSCULAR RABIES VACCINATION Subawa, A. A. Ngurah; Sutirta Yasa, I Wayan Putu; Astawa, Nyoman Mantik
BALI MEDICAL JOURNAL Vol 3 No 3 (2014)
Publisher : BALI MEDICAL JOURNAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.676 KB)

Abstract

Background: Rabies is a cause of death to people within 100% of Case Fatality Rate. Approximately 55.000 people died because of rabies each year, the vast majority of these deaths happen in Asia and Africa. This study aims to find out comparative immune responses of intradermal (ID) and intramuscular (IM) vaccination in children. Method: This was an experimental study to determine antibody response of ID and IM rabies vaccines with randomized pre and posttest control group design. ID and IM vaccination were carried out in 4 replication for each group. A number of 16 children were recruited for each group. Enzyme Linked Immunosorbent Assay (ELISA) was applied to determine titers antibody on day 0, 7, 21, and 28 after vaccination. Results: This study found that titer antibody induced by ID vaccination was lower than IM vaccination. However, the different is not statistically significant in both groups  (p > 0.05). Titers antibody on day 7 after vaccination were 3.08 ± 2.09 IU/ml intradermally and 4.22 ± 3.02 IU/ml intramuscularly. On day 21 and 28 after intradermal vaccination, titers antibody were 6.78 ± 3.52 IU/ml and 12.53 ± 5.92 IU/ml, respectively. Intramuscularly, antibody titers were 9.76 ± 4.86 IU/ml on day 21 and 14.98 ± 7.76 IU/ml on day 28. Conclusion: ID vaccination is safe and can be used as an alternative vaccination for rabies in human. In addition, 0, 7, 21 ID vaccination methods can be recommended for use to control rabies cases in Indonesia because that methods induce protective immune response.
Co-Authors A. A. G. P. Wiraguna A.A.G. Sudewa AAG Budhitresna AAG Putra Ahmad Hamim Sadewa Aida Lousie Tenden Rompis Alberto Agustinho Pereira Da Costa Joao Anak Agung Ayu Mirah Adi Anak Agung Bagus Bramardipa Anak Agung Gede Sudewa Djelantik Anak Agung Keswari Krisnandika Anak Agung Ngurah Subawa Anak Agung Oka Wijaya Anak Agung Sagung Kendran and R. Kusnandi Andika Budi Kurnianto Anwar Santoso Arthawan Arthawan Bayu Setiabudi Berata , I Ketut Chandra Yowani Dewa Ayu Agus Sri Laksmi DWI SURYANTO Faiziah - G.A.M.K. Dewi Gusti Ayu Mayani Kristina Dewi Gusti Ayu Yuniati Kencana Gusti Ngurah Narendra Putra HARTANINGSIH - Hartaningsih . I D. N. Wibawa I Dewa Made Sukrama I Gede Ngurah Harry Wijaya Surya I GEDE PUTU WIRAWAN I Gusti Agung Arta Putra I Gusti Agung Ayu Suartini I Gusti Agung Dewi Sarihati I Gusti Agung Trisna Windiani I Gusti Ayu Putu Eka Pratiwi I Gusti Made Krisna Erawan I Gusti Ngurah Kade Mahardika I Gusti Ngurah Sudisma I K. Sukardika I Kadek Swastika I Ketut Berata I Ketut Eli Supartika I Ketut Junitha I Ketut Suada I Ketut Suada I Ketut Suastika I Ketut Suastika I KETUT SUATA I Ketut Suatha I Ketut Suwiyoga I Made Damriyasa I Made Dwinata I Made Galih Diparayoga I Made Kardena I Made Subrata I Made Sudarmaja I Made Sukada i Nengah Wandia I Nyoman Agus Bagiada I Nyoman Polos I Nyoman Suarsana I Nyoman Suartha I W. Wita, I W. I Wayan Bebas I Wayan Gorda I Wayan Masa Tenaya I Wayan Megadhana I Wayan Putu Sutirta Yasa I Wayan Suardana I Wayan Wita I.A.P. Apsari I.B.K. Suardana I.H. Utama I.W. Batan Ida Ayu Pasti Apsari Ida Bagus Kade Suardana Ida Bagus Made Oka Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya Ida Bagus Suardana Ignatius Ferdi Yuatmadja Inna Narayani K. Sri Marhaeni Julyasih K. Suata K. Sukardika Kadek Karang Agustina Ketut Budiasa Ketut Santhia Adhy Putra Ketut Suata Luh Dewi Anggreni LUH PUTU AGUSTINI Luh Putu Agustini Luh Putu Wrasiati M.D. Rudyanto M.Pd S.T. S.Pd. I Gde Wawan Sudatha . Marissa Divia Dayanti Mudinillah, Adam N. T. Suryadhi Ngakan Putu Anom Harjana Ni Luh Putu Eka Diarthini NI LUH PUTU MANIK WIDIYANTI Ni Made Krisna Dewi Ni Made Suaniti NINING HARTANINGSIH Nyoman Agus Bagiada Nyoman Tigeh Suryadi Oka Lely Palagan Senopati Sewoyo Putu Ayu Asri Damayanti Putu Ayu Sisyawati Putriningsih Putu Sudiarta Rasmaya Niruri Rasmaya Niruri S. Soetjiningsih, S. S. Sotjiningsih S.K. Widyastuti sang gede purnama Siti Maryam Soetjiningsih - Soetjiningsih . Sri Kayati Widyastuti Sri Wahjuni SUMARNO Sutjahjo Suherman, Sutjahjo Suwarno - T. Sari Nindia Tjok Gede Oka Pemayun, Tjok Gede Oka Tjokorda Gde Agung Suwardewa Wayan Tunas Artama Wimpie I Pangkahila Yasunobu Matsumoto Yosevangelika Hutabarat Yoshihiro Hayashi