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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Perikanan dan Kelautan Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Indonesian Journal of Geography Maspari Journal Biospecies Bumi Lestari Jurnal Ilmu Lingkungan JURNAL TEKNOLOGI LINGKUNGAN Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Maspari Journal Journal of Degraded and Mining Lands Management Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Jurnal Kimia dan Kemasan Jurnal Pariwisata INTENSIF: Jurnal Ilmiah Penelitian dan Penerapan Teknologi Sistem Informasi Jurnal Biologi Tropis Indonesian Journal of Chemistry Biosaintifika: Journal of Biology & Biology Education Jurnal Ecolab Jurnal Segara Jurnal Pengelolaan Lingkungan Berkelanjutan (Journal of Environmental Sustainability Management) Jurnal Kebijakan Sosial Ekonomi Kelautan dan Perikanan Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Advances in Tropical Biodiversity and Environmental Sciences Grouper: Jurnal Ilmiah Perikanan Jurnal Kimia dan Kemasan Makara Journal of Science Jurnal Ilmu Kehutanan
Hefni Effendi
1) Departemen Manajemen Sumberdaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan, IPB University, Bogor, Indonesia; 2) Pusat Penelitian Lingkungan Hidup, IPB University, Bogor, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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Journal : Makara Journal of Science

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Highly Sensitive Phenol Biosensor Utilizing Selected Bacillus Biofilm Through an Electrochemical Method Ariyanti, Dita; Iswantini, Dyah; Sugita, Purwantiningsih; Nurhidayat, Novik; Effendi, Hefni; Ghozali, Ali Aulia; Kurniawan, Yehezkiel Steven
Makara Journal of Science Vol. 24, No. 1
Publisher : UI Scholars Hub

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Abstract

An eco-friendly phenol biosensor from Bacillus biofilm was prepared and investigated. The biofilm, which produced tyrosinase enzyme, was successfully immobilized on a screen-printed carbon electrode surface. A total of 72 Bacillus isolates were utilized because of their capability to produce tyrosinase enzyme in tyrosine media. Among them, Bacillus isolate code 100 was selected because it produced an adequate amount of tyrosinase enzyme and a high potentiostat current. The response surface methodology was also used to optimize the phenol sensing condition through an electrochemical method. Results showed that the optimum condition was achieved after 6 days on a phosphate buffer solution (pH of 8), with an optical density of 0.33. Furthermore, the limits of detection and quantification were 3.0 and 13 ng/L, respectively. The measurements of precision yielded a relative standard deviation of < 5%, which is remarkable. Although the biosensor material was used for 35 days, the current throughout was still maintained at 90%, indicating that the evaluated biosensor material has the potential to be used for phenol monitoring on environmental samples in the near future.
Co-Authors - - mardiyana - - nurjanah . Chaidir A. Zaenal Mustopa A. Zaenal Mustopa Adi Sulaksono Aditya Herry Emawan Agustina Sinuhaji Ahmad Jamhari Rahmawan Ali Mashar Aloysius Adimas Kristianiarso Andrian Rizaldy Azhar Audra Ligafinza Audra Nur Ayub Sugara B Sartono Bagus Amalrullah Utomo Bagus Amalrullah Utomo Bq Tri Khairani Ilhami Bq Tri Khairina Ilhami Budi Kurniawan Budi Nurtama Budi Nurtama Chaidir Chaidir Charles Parningotan Haratua Simanjuntak Cut Meurah Nurul 'Akla Damar, Ario Dea Fauzia Lestari Dedi Soedarma Dedi Soedarma Dedi Soedharma Dedi Soedharma DEDI SOEDHARMA Dewanti Pratiwi Dietriech G. Bengen Dietriech Geoffrey Bengen Dino Rimantho Dita Ariyanti Ditta Ayu Anggraini Dwi Suci Indah Permata Sari Dyah Iswantini Dyah Muji Rahayu Enan M Adiwilaga Eriyatno . Erlangga Erliza Noor Erniati Etty Riani Eva Cristine Ronauli F Farlina Fitri Ariani Flandrianto Sih Palimirmo Fredinan Yulianda Gatot Prayoga Gatot Prayoga Gatot Yulianto Gede Iwan Setiabudi Ghozali, Ali Aulia Giri Maruto Darmawangsa Gunawan Gunawan Gunawan Pratama Yoga Hadi Supardi Hari Wijayanto Harum Farahisah Harum Farahisah Herman Yulianto I Wayan Nurjaya Imamshadiqin Iman Rusmana Imanullah Irza Arnita nur Kurniawan, Yehezkiel Steven Luisa Febrina Amalo Luluk Dwi Wulan Handayani Majariana Krisanti Marfian Dwidima Putra Maryono Maryono Maulid Wahid Yusuf Melanie Cornelia, Melanie Melki . Melki Melki Meutia Samira Ismet Muhammad Mukhlis Kamal MUJIZAT KAWAROE MUJIZAT KAWAROE Mujizat Kawaroe Mujizat Kawaroe Mujizat Kawaroe Mujizat Kawaroe Mukti Ali Mursalin Idris Mursalin Mursalin Mursalin Mursalin Nabil Zurba Neneng Marlian Neneng Sri Hendra Neviaty P Zamani Neviaty P. Zamani Neviaty P. Zamani NEVIATY PUTRI ZAMANI Niken TM Pratiwi Niken Tunjung Murti Pertiwi Niken Tunjung Murti Pratiwi Noveldesra Suhery Novera Nirmalasanti Novik Nurhidayat Ocky Karna Radjasa Ocky Karna Radjasa Prasetyo, Kunandar Purwantiningsih Sugita Purwanto, Yanuar Jarwadi Qadar Hasani Rachma Venita Rachmad Caesario Rahman Rahman Rahman Rahman Rahmat Pangestu Rais Sonaji Rebo Elfida Karo-Karo Refa Riskiana Rezi Apri Ririn Setyowati Rizal Syarief Rizal Syarief Rizki Novia Rahmi Romi Seroja Rozirwan . safrina dyah hardiningtyas Saiful Adhar Sigid Hariyadi Sigit Winarno Siti Rosa Oktavia Sulistiono Suprihatin Suprihatin Suprihatin Suprihatin Suprihatin Suprihatin Suprihatin Suprihatin Suria Darma Tarigan Surya Cipta Ramadhan Kete Taryono Taryono TATI NURHAYATI Tri Permadi Warsiki, Endang Widiatmaka . Widyatmoko Widyatmoko Wiwin Ambarwulan Wiwin Ambarwulan Yonvitner - Yudhi Amrial Yudho Andika Yudho Andika Yudi Setiawan Yunandar Yunandar Yuni Yolanda Yusli Wardiatno Zidni Ilma Palupi